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Immersion Oils

for light microscopy, fluorescence microscopy, inclined instruments and projection equipment

Cargille Immersion Oils

Cargille Immersion Oils are in full accord with the FDA requirements.
To date, no other immersion oils meet the FDA criterias.
Cargille Immersion Oils are PCB (polychlorinated biphenyls) free.

Immersion Oil Selection Guide

Standard Light Microscopy
Type B, with a viscosity of 1250 cSt (centistokes), meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. Type B, with 1250 cSt, is thick enough for viewing multiple slides with one application, thus saving time during batch processing. Cargille Immersion Oil B, can be blended for intermediate viscosities.
Cargille Immersion Oil Specifications

Automated Hematology Systems
Type 300 has been specifically designed to meet results for optimum optical imaging and mechanical processing, medium viscosity of 300 cSt.
Cargille Immersion Oil Specifications

Fluorescence Microscopy
Fluorescence microscopy require special immersion oils with extremely low fluorescence. Type LDF exhibits extremely low fluorescence. Type FF is virtually fluorescence free, but not ISO compliant. Viscosities for type LDF and FF are 500 cSt and 150 cSt respectively.

For non-critical fluorescence application, one could also consider immersion oil type B which both has low fluorescence.
Cargille Immersion Oil Specifications

Why do Cargille Immersion Oils Meet the DIN 58 884?
(Deutsches Institut Für Normung e.V.)
Refractive index, dispersion and temperature standardizations are the key specifications for optical clarity and high resolution. (Viscosity and other physical properties are directed toward specific applications and convenience of the user.)
Immersion oil specifications must meet the design requirements of old instruments and become the design criteria for new instruments. Without these specifications, it would be much harder for the microscopist to find the proper oil for his or her particular instruments' requirements and optical imagery would suffer.
The specifications adopted by DIN resulted from their study of instrument requirements of many manufacturers. The proposed specifications were submitted for review, with Cargille Laboratories serving as liaison for the American manufacturers.
The present trend toward tighter specification for optical properties is under a program of the International Standards Organization (ISO).

Unique Applications - Special Properties
Because of widespread availability, consistent quality, and close specifications, Cargille Oils are used as:

  • A temporary mounting media
  • Fluid mounts (permitting specimen rotation)
  • Calibration liquids
  • Optical coupling fluids
  • Combination protective coating and examination fluid
  • Transparency medium for translucent materials
  • Filter clearing agent (as recommended by Millipore Corp.)

Important Comments
Cargille Immersion Oils are supplied as a microscopy accessory by American Optical and Bausch & Lomb, and accepted for use by all foreign microscope manufacturers. Cargille Immersion Oils are synthetic hydrocarbons, natural petroleum derivatives that contain no solvents and no PCB's. These oils are stable, non-drying, odorless, safe for lens and handling, and easily removable. MSDS sheets are available.

immersion oil

Made in USA
Prod # Description Unit Price Order / Quote
U MSDS Low viscosity (150cSt), low fluorescence.
19556 Cargille Immersion Oil, Type B, high visc, 120ml (4 oz.) each $23.00
U MSDS High viscosity (1250cSt), low fluorescence.
19563 Cargille Immersion Oil, Type 300, medium viscosity, 120ml (4 oz.) each 23.00
U MSDS Medium viscosity (300cSt).
19564-L Cargille Immersion Oil, Type LDF,fluorescence applications , 30ml (1 oz.) each 15.00
U MSDS Extremely low fluorescence, Medium viscosity (500cSt).

Citifluor Immersion Oil

Citifluor AF87

Citifluor AF87 is a medium viscosity immersion oil with a refractive index of ~1.52 at room temperature (20°C). This oil is ideal for confocal laser scanning microscopy and FISH label with DAPI and Cy dyes. Citifluour AF87 should be stored at room temperature. Low temperature may cause sediment to develop and reduce performance.

Prod # Description Unit Price Order / Quote
19476-A Citifluor Non-Flourescent Immersion Oil, AF87, 10 ml each $80.00

lens tissue

Box and Booklet shown

PELCO® Optical Lens Tissue

Fully comparable to Ross optical lens tissue

The PELCO® Optical Lens Tissue is a high quality optical lens tissue for lenses, optical instruments, electron microscopy and similar applications. PELCO® Optical Lens Tissue is fully lab tested and is comparable to the Ross lens tissue paper in regards to structure, softness, properties and cleaning capabilities. It is a direct replacement for Ross lens tissue and fully compatible for all applications. The tissue is made of 100% new stock, free of minerals and vegetable filler. This lens tissue does not lint or scratch and will not disintegrate under vigorous manipulation and is acid-free with excellent wicking properties.

High quality optical lens tissue for applications such as:

  • Cleaning microscopes, electron microscope parts, optical components, lenses and delicate instruments
  • Wet or dry cleaning
  • Displays
  • Wrapping and protecting optical components and delicate parts
  • Skimming liquid surfaces

The size of the PELCO® Optical Lens Tissue is 114 x 127mm (4-1/2" x 5") and is available in a box/1000 sheets or in a pack with 6 booklets of 50 sheets each.

Prod # Description Unit Qty Price Order / Quote
806 PELCO® Optical Lens Tissue, 114x127mm, box of 1000 sheets 1 box 1-9 $33.55
10+ 31.87
806-1 PELCO® Optical Lens Tissue, 114x127mm, booklets of 50 sheets pkg/6 booklets 1-9 13.35
10+ 12.68