Embedding Kits

Araldite 502 Embedding Kits

The Araldite 502 Kit with BDMA accelerator is equally efficient as the same kit using DMP-30. BDMA has a lower viscosity and diffuses more rapidly into tissues, porous specimens and rough surfaces. The Araldite 502 resins have better beam stability than the Eponate resins. BDMA is used in 3% concentrations versus 1.5 to 2% for DMP-30. Araldite kit may be used alone or mixed with other epoxies. Curing times are 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave^® Pro+.

PELCO® Technical Notes, Araldite 502 Embedding Kits (263KB PDF)

1 Glauert AM, Glauert RH: Araldite as an embedding medium for electron microscopy. J Biophys Biochem Cytology, 4:191-194, 1958.
2 Glauert AM: Fixation, dehydration, and embedding of biological specimens. Practical Methods in Elec Micr, 3(1). Glauert AM, ed., North-Holland, Amsterdam, 5th reprint 1975.

Eponate 12™ Kit, with BDMA

Aliphatic epoxy resin based on di- and tri-glydicyl ethers of glycerol with a significantly lower viscosity compared to Araldite 502. BDMA (<3%, see notes preceding) is used as the accelerator since it has lower viscosity and diffuses into tissues more rapidly. (See references below).

The viscosity of Eponate™ 12 at 25ºC is 15-21cP, WPE is 140–145 and Specific Gravity is 1.15–1.22g/ml. Other kit viscosities at 25ºC include DDSA (290cP) and NMA (225-315cP). Eponate™ 12 is hygroscopic.

PELCO® Technical Notes, PELCO® Eponate 12™ Kit with DMP-30 and BDMA (276KB PDF)

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Cg, I	Eponate 12™ Resin, 450g (18005) SDS (92KB PDF)
I	DDSA, 450g (18022) SDS (134KB PDF)
C, I	NMA, 450g (18032) SDS (110KB PDF) - - Hazard Shipping Fees
C, T, I, F	BDMA, 25g (18241) SDS (137KB PDF)

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Cg, I	Eponate 12™ Resin, 450g (18005) SDS (92KB PDF)
I	DDSA, 450g (18022) SDS (134KB PDF)
C, I	NMA, 450g (18032) SDS (139KB PDF) - - Hazard Shipping Fees
T	DMP-30, 25g (18042) SDS (117KB PDF)

Poly/Bed® 812 Embedding Kit, with DMP-30 Poly/Bed® 812 for electron microscopy and histology for studies requiring beam stability, low shrinkage and good contrast. Epoxy formulations are lower in viscosity than Araldite formulations, and hence provide better penetration into tissue samples. Instructions included. - Hazard Shipping Fees Prod # Description Unit Price Order / Quote 18109 Poly/Bed® 812 Kit (w/DMP-30) each $522.75 Qty: Cg, I Poly/Bed® 812, 500g SDS (PDF 100KB) I DDSA, 450g (18022) SDS (134KB PDF) C, I NMA, 450g (18032) SDS (139KB PDF) - - Hazard Shipping Fees T DMP-30, 100g SDS (117KB PDF)

	Poly/Bed® 812 Resin Test Kit contains BDMA as accelerator Contains BDMA in place of DMP-30 for faster infiltration. Instructions, supplies, syringes provided for leftovers. Mixture bottle provided.

Prod #	Description	Unit	Price	Order / Quote
18107	Poly-Bed® Test Kit	each	$251.75	Qty:

I	Poly/Bed® 812, 50ml SDS (100KB PDF)
I	DDSA, 40ml SDS (134KB PDF)
T	NMA, 25ml SDS (139KB PDF)
C, T, I, F	BDMA, 3.5ml SDS (100KB PDF)

"Spurr" Low Viscosity Kits ERL 4221 has replaced ERL 4206. ERL 4221 has a higher viscosity than the previously used ERL 4206. Spurr AR, 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruc Res, 26, p. 31. Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, V 14, No 4, July 2006 The Spurr Kit gives excellent penetration for tough materials particularly in the studies of botanical, mineral and other hard biological specimens. When the mixture is kept at room temperature for 24 hours its viscosity will increase to 140cP, as is the case with other resins. When fully formulated, the Spurr’s resin will store in a deep-freeze for two months and at room temperature for two days. Curing is done at 70ºC for about 8 hours (see our Microwave system for significant time saving during epoxy processing). PELCO® Technical Notes for Low Viscosity Kit (1.54MB PDF) Prod # Description Unit Price Order / Quote 18300-4221 Low Viscosity "Spurr" Kit each $123.50 Qty: Cg ERL 4221 (replaces ERL 4206), 225ml (18306-4221) SDS (95KB PDF) I DER® 736, 250g (18310) SDS (104KB PDF) I NSA, 450g (18301) SDS (100KB PDF) C DMAE, 25g (18315) SDS (138KB PDF)

	LR White Resin
Prod # Description Unit Price Order / Quote 18180 LR White Resin, Soft, 500ml each $242.40 Qty:

I - (Resin) SDS (123KB PDF) - (Catalyst) SDS (162KB PDF) - - Hazard Shipping Fees

Resin with initiator (BPO) packaged separately. Accelerator #18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR® as cover (#10506 or #10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds). * LR White Soft is not recommended for electron microscopy.

Prod #	Description	Unit	Price	Order / Quote
18181	LR White Resin, Medium, 500ml	each	$249.75	Qty:

I - (Resin) SDS (123KB PDF) - (Catalyst) SDS (162KB PDF) - - Hazard Shipping Fees

Resin with initiator (BPO) packaged separately. Accelerator #18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR® as cover (#10506 or #10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds). Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions could be seen on the complimentary surface. Epon sections show relatively smooth surfaces.18 Micrographs appear similar to freeze-etched images but scaled to nm instead of µm. Viscosity ~= 8cP 18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470 Landmark Paper, Freeze-Fracture Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957 Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987. Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, V. 2, (Hayat MA, ed) PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod #	Description	Unit	Price	Order / Quote
18182	LR White Resin, Hard, 500ml	each	$236.50	Qty:

I - (Resin) SDS (123KB PDF) - (Catalyst) SDS (162KB PDF) - - Hazard Shipping Fees

Resin with catalyst packaged separately. Accelerator #18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly close BEEM® Capsules and PTFE Molds with ACLAR® as cover (#10506 or #10508) work well at 65°C. Widely used because of excellent immunolabeling results. PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod #	Description	Unit	Price	Order / Quote
18185	Accelerator for LR white, 10ml	each	$50.10	Qty:

T- SDS (133KB PDF)

PELCO® Technical Notes, PELCO® Glycol Metbhacrylate (GMA) Kit (166KB PDF)

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JB-4^® Plus Embedding Kit

Kulzer Technovit^® 7100 Acrylic System (GMA)

Kulzer Technovit^® 7100 is a hydrophilic acrylic resin system based on HEMA (2-hydroxyethyl methacrylate) GMA (glycol methacrylate) suitable for use with materials to be sectioned on a microtome – including biological samples as well as plastics, foils, wires, etc. The resin is cold-curing and is both stable and elastic enough to allow slicing of sections down to 1μm thickness. Applications for Kulzer Technovit^® 7100 include histological staining and enzyme detection for light microscopy.

#813-590 Complete System Inlcudes:

Hardener 1, (5) x 1g SDS (330KB PDF)
Hardener 2, (1) x 40ml SDS (150KB PDF)
Liquid, (1) x 500ml SDS (164KB PDF)

Kulzer Technovit^® 8100 Plastic Embedding System (GMA)

Plastic Sections for Light Microscopy and Immunohistochemistry

Kulzer Technovit^® 8100 is a HEMA-based plastic-embedding system (Glycol Methacrylate - GMA) for studies with light microscopy. It is suitable for embedding all tissues in medicine, zoology and botany. Thin resin sections provide for higher resolution microscopy that is not possible with paraffin sections. Sections of decalcified or briefly decalcified iliac crest biopsies and implanted biomaterials can be used for more than just histological staining; may also be used for enzyme chemistry and immunohistochemistry.

Benefits:

• Polymerization is oxygen-sensitive
• Specifically developed for cold polymerization (10°C to 0°C)
• Low shrinkage artefacts, excellent tissue morphology
• Routine staining, enzyme detection and immunohistochemistry possible
• Hematological iliac crest biopsies do not need to be decalcified

#813-800 Complete System Includes:

Hardener 1 SDS (304KB PDF)
Hardener 2 SDS (141KB PDF)
Liquid SDS (173KB PDF)

Kulzer Technovit^® 9100 Plastic Embedding System (MMA)

Kulzer Technovit^® 9100 is a plastic embedding system based on MMA (methyl methacrylate). It is used in medicine, botany and zoology.

Kulzer Technovit^® 9100 was specifically developed for the embedding of mineralized tissues (e.g,. bone) as well as soft tissue with an expanded study spectrum in light microscopy. The deplasticized sections are for histological overview staining, enzyme chemistry and immunohistological studies, including in-situ hybridization. Thin sections for immunohistochemistry can be stuck to glass object holders and deplasticized.

Fields of Application:

Hard-cutting technique for making thin layers
Examples: iliac crest biopsies; smaller, spongy and compact bone tissue specimens.

Division thin section technique (division procedure in point contact technology)
Examples: tooth/jaw areas with and without implants; non-cemented endoprostheses with shaft bones.

Combined division-thin section technique and hard-cutting technique (target preparation)
Examples: boundary layer and environment assessment for metal implants and non-cemented endoprostheses.

Tissues that cannot be cut are: teeth-bearing jaw sections with fillings, crowns and bridges, thick corticalis, implant-bearing (metal or ceramic) jaw or long bones, or brittle, hyper-mineralized bones.

Benefits:

• Polymerization below freezing
• Uniform block hardening
• The PMMA block remains transparent
• Better results with regard to cutting and staining due to 9100 containing a hydrophilizing agent
• Can be used for thin section and the sawing and cutting techniques
• Enzyme histology and immunohistochemistry as well as in-situ hybridization possible (sections)

#813-900 Complete System Includes:

Basic Solution, (1) x 120g SDS (224KB PDF)
PMMA Powder, (8) x 1g SDS (260KB PDF)
Hardener 1, (1) x 10ml SDS (304KB PDF)
Hardener 2, (1) x 5ml SDS (184KB PDF)
Regulator SDS (157KB PDF)