Embedding Kits

Eponate 12™ Embedding Kit, with BDMA

First use of Epon 812 in U.S.A.:
Finck H, 1960. Epoxy resins in electron microscopy. J Biophys Biochem Cytol, 7, p. 27.
Luft JH, 1961. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol, 9, p. 409.

18012

$270.35

Eponate 12™ Resin, 450g (18005) SDS (92KB PDF)

Cg, I

DDSA, 450g (18022) SDS (134KB PDF)

C, I

NMA, 450g (18032) SDS (110KB PDF) -

BDMA, 25g (18241) SDS (137KB PDF)

C, T, I, F

Eponate 12™ Embedding Kit, with DMP-30

PELCO® Technical Notes, PELCO® Eponate 12™ Kit with DMP-30 and BDMA (276KB PDF)

18010

Eponate 12 Kit (DMP-30 catalyst)

$257.60

Eponate 12™ Resin, 450g (18005) SDS (92KB PDF)

Cg, I

DDSA, 450g (18022) SDS (134KB PDF)

C, I

NMA, 450g (18032) SDS (139KB PDF) -

DMP-30, 25g (18042) SDS (117KB PDF)

*NMA Hardener in increased amounts, in the embedding mixture, make a harder block.

Eponate 12™ –Araldite 502 Kit with BDMA

PELCO® Technical Notes, PELCO® Eponate 12™ Kit with DMP-30 and BDMA (276KB PDF)

18028

Eponate 12™ –Araldite 502 Kit with BDMA

$280.20

PELCO® Technical Notes, PELCO® Araldite 502 Kit with DMP-30 and BDMA (PDF File)

Cg, I	Eponate 12™ Resin, 450g (18005) SDS (92KB PDF)
T, I	Araldite 502 Resin, 450g (18060) SDS (129KB PDF)
I	DDSA, 450g (18022) SDS (134KB PDF)
C, T, I, F	BDMA, 25g (18241) SDS (137KB PDF)

Eponate 12™ –Araldite 502 Kit with DMP-30

PELCO® Technical Notes, PELCO® Eponate 12™ Kit with DMP-30 and BDMA (276KB PDF)

18030

Eponate 12™ –Araldite 502 Kit with DMP-30

$264.85

Glycol methacrylate (GMA) Kit

Cg, I	Eponate 12™ Resin, 450g (18005) SDS (92KB PDF)
T, I	Araldite 502 Resin, 450g (18060) SDS (129KB PDF)
I	DDSA, 450g (18022) SDS (134KB PDF)
T	DMP-30, 25g (18042) SDS (117KB PDF)

Glycol Methacrylate (GMA) Kit

18350

$78.00

F, I, T	Glycol Methacrylate (GMA), 450ml (18091) SDS (102KB PDF)
F, I, T	n-Butyl Methacrylate, 225ml (18035) SDS (121KB PDF)
O	Benzoyl Peroxide Paste (50%), 15g (18097) SDS (159KB PDF)

PELCO® Technical Notes, PELCO® Glycol Metbhacrylate (GMA) Kit (166KB PDF)

JB-4^® Plus Embedding Kit

JB-4^® Plus Embedding Kit for light microscopy is a water soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. Its cooler acting accelerator system offers greater heat protection of tissue components. It yields semi-thin sections (0.5 - 2µm) with excellent morphological preservation. Crystal clear non-yellowing casts are obtained at room temperature in 90 minutes or less.

Prod #

Description

Unit

Price

Order / Quote

18040

JB4^® Plus Embedding Kit

each

$485.50

Qty:

F, I, T

Monomer Solution A, 500ml (18043) SDS (124KB PDF)

F, I, T

Accelerator Solution B, 15ml (18044) SDS (88KB PDF)

Catalyst, 8g (18045) SDS (153KB PDF) -

PELCO® Technical Notes for Low Viscosity Kit (1.54MB PDF)

"Spurr" Low Viscosity Kits

ERL 4221 has replaced ERL 4206. ERL 4221 has a higher viscosity than the previously used ERL 4206.

Spurr AR, 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruc Res, 26, p. 31.

Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, V 14, No 4, July 2006

The Spurr Kit gives excellent penetration for tough materials particularly in the studies of botanical, mineral and other hard biological specimens. When the mixture is kept at room temperature for 24 hours its viscosity will increase to 140cP, as is the case with other resins. When fully formulated, the Spurr’s resin will store in a deep-freeze for two months and at room temperature for two days. Curing is done at 70ºC for about 8 hours (see our Microwave system for significant time saving during epoxy processing).

Prod #

Description

Unit

Price

Order / Quote

18300-4221

Low Viscosity "Spurr" Kit

each

$123.50

Qty:

Cg	ERL 4221 (replaces ERL 4206), 225ml (18306-4221) SDS (95KB PDF)
I	DER^® 736, 250g (18310) SDS (104KB PDF)
I	NSA, 450g (18301) SDS (100KB PDF)
C	DMAE, 25g (18315) SDS (138KB PDF)

Lowicryl^® HM-20 MonoStep^® (non polar)

For Sale in U.S.A. Only

18174

Lowicryl^® HM-20 MonoStep^® (non polar), 225g Pre-mixed, ready to use embedding media

$471.75

Poly/Bed^® 812 for electron microscopy and histology for studies requiring beam stability, low shrinkage and good contrast. Epoxy formulations are lower in viscosity than Araldite formulations, and hence provide better penetration into tissue samples.

I, O, T - SDS (121KB PDF) -

- Hazard Shipping Fees

Poly/Bed^® 812 Embedding Kit, with DMP-30

-	Hazard Shipping Fees

18109

Poly/Bed^® 812 Kit (w/DMP-30)

$522.75

Poly/Bed^® 812, 500g SDS (PDF 100KB)

Cg, I

DDSA, 450g (18022) SDS (134KB PDF)

C, I

NMA, 450g (18032) SDS (139KB PDF) -

DMP-30, 100g SDS (117KB PDF)

* Instructions included.

Test Kits

Poly/Bed^® 812 Resin Test Kit

contains BDMA as accelerator

Contains BDMA in place of DMP-30 for faster infiltration. Instructions, supplies, syringes provided for leftovers. Mixture bottle provided.

18107

$251.75

I	Poly/Bed^® 812, 50ml SDS (100KB PDF)
I	DDSA, 40ml SDS (134KB PDF)
T	NMA, 25ml SDS (139KB PDF)
C, T, I, F	BDMA, 3.5ml SDS (100KB PDF)

LR White Resin

18180

LR White Resin, Soft, 500ml

$242.40

I - (Resin) SDS (123KB PDF) - (Catalyst) SDS (162KB PDF) -

LR White Resin, Medium, 500ml

Resin with initiator (BPO) packaged separately. Accelerator #18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM^® Capsules and PTFE Molds with ACLAR^® as cover (#10506 or #10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds).

* LR White Soft is not recommended for electron microscopy.

18181

$249.75

I - (Resin) SDS (123KB PDF) - (Catalyst) SDS (162KB PDF) -

18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470

Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions could be seen on the complimentary surface. Epon sections show relatively smooth surfaces.18 Micrographs appear similar to freeze-etched images but scaled to nm instead of µm. Viscosity ~= 8cP

Landmark Paper, Freeze-Fracture
Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957

Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987.
Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, V. 2, (Hayat MA, ed)

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

18182

LR White Resin, Hard, 500ml

$236.50

I - (Resin) SDS (123KB PDF) - (Catalyst) SDS (162KB PDF) -

Resin with catalyst packaged separately. Accelerator #18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly close BEEM^® Capsules and PTFE Molds with ACLAR^® as cover (#10506 or #10508) work well at 65°C. Widely used because of excellent immunolabeling results.

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

18185

Accelerator for LR white, 10ml

$39.15