in 

SEM Supplies & Accessories Overview

Serial Block-Face Electron Microscopy
(SBEM) Supplies & Accesories


PELCO BioWave Pro
Sample Preparation
16145 16145
16145

Tools & Consumables
PELCO® Conductive Liquid Silver Paint
Conductive Adhesives
PELCO 18 SEM Pin Mount Storage Holder and Box
Cryo Pin Storage Boxes
PELCO® SEM Sample Stub Vacuum Desiccator 16179
SBEM Sample Storage
 

 

Sample Preparation for SBEM

For the most consistent and reliable SBEM preparation, the PELCO BioWave® Pro+ is the answer.

From tissue to block in less than 6 hours!

PELCO BioWave Pro   PELCO BioWave Pro

PELCO BioWave Pro
SBEM Images: Rick Webb, University of Queensland




Tools & Consumables for SBEM


Made in USA
dimensions 16145 16145 

Prod # Description Unit Price Order / Quote
Serial Block-Face Pin Mounts for Thermo Scientific VolumeScope™ Each pin stub is 8.0mm in diameter with a 2.0mm specimen flat and grooved area for improved handling.
16145 Thermo Scientific VolumeScope™ Pin Stub with Specimen Flat pkg/10 $20.50
Qty:
pkg/ 50 97.50
Qty:
pkg/ 100 185.00
Qty:



Made in USA
16950-1
16950-1
#16950-1

Prod # Description Unit Price Order / Quote
Gatan 3View SEM Pin Stubs These beveled pin stubs are used to mount the sample for the automated serial-sectioning of a block face inside the Gatan 3View system. The top beveled surface where the sample is mounted comes in two sizes 1.4mm (#16950-1) and 2.4mm (#16950-5). The stem on the pin is 2mm in diameter and the overall pin length is 12.5mm. The stubs are sold in packs of 10 and 50.
16950-1 Gatan 3View Pin Stubs, 1.4mm flat top surface, 2mm pin x 12.5mm H, aluminum pkg/10 $16.00
Qty:
pkg/ 50 76.00
Qty:
16950-3 Gatan 3View Pin Stubs, 2.4mm flat top surface, 2mm pin x 12.5mm H, aluminum pkg/10 16.00
Qty:
pkg/ 50 76.00
Qty:
16950-1 16950-3
#16950-3




PELCO 18 SEM Pin Mount Storage Holder and Box

Cryo Pin Storage Boxes

These clear, styrene storage boxes contain a dense, closed cell foam insert that have slots cut out. The slots can be used to hold pins such as the Cryo Specimen Pins (#16948, 16949) or Gatan 3View Pins (#16950-1, 16950-3)

Pins shown are sold separately.

Prod # Description Unit Price Order / Quote
16951 Styrene Storage Box, 2 x 2 x 3/4" for 10 pins each $16.50
Qty:
16952 Styrene Storage Box, 4-5/8 x 3-1/2 x 1" for 50 pins each 22.00
Qty:




PELCO® Pro General Purpose Tweezers

Angled serrated tips provide excellent grip in the grooved area of the Serial Block-Face Pin Stub (#16145) above.


PELCO Pro General Purpose Tweezers style 124

Prod # Description Style Length Metal Points
Width x Thickness
Qty Price Order / Quote
5817 PELCO® Pro General Purpose Tweezers, Strong Serrated Tips & Grips 124 150mm NM-SS 0.81 x 0.56mm 1+ $20.40
Qty:


PELCO Pro General Purpose Tweezers style 119B

Prod # Description Style Length Metal Points
Width x Thickness
Qty Price Order / Quote
5802 PELCO® Pro General Purpose Tweezers, Fine Serrated Tips & Grips, Bent 119B 150mm NM-SS 0.40 x 0.20mm 1+ $21.50
Qty:





specimen embedding mold pyramid embedding mold

PELCO® Pyramid-Tip Mold

Excellent for sample preparation of specimens which are difficult to orient. The tips easily and quickly attach to plastic cylinders. When tip is mounted it is identical in dimensions to a BEEM® 00 size capsule. Blue silicone rubber.
Maximum temperature: 218°C (425°F).
See our Cyanoacrylate Fast-Glue and Mounting Cylinders.

Number of Cavities: 30
Numbering: OUTSIDE of Cavities, 1-30
Mold Size: 61 x 72 x 6mm thick

Prod # Description Unit Price Order / Quote
10585 PELCO® Pyramid-Tip Mold each $14.95
Qty:


   
embedding mold
embedding mold

PELCO® 3mm Square Face Mold

for embedding larger specimens, uses less resin

With this mold a larger specimen may be placed in a wider tip area resulting in a larger initial section. Once solidified the block may be glued to the end of a plexiglas rod or other holding device. (See Mounting Cylinders.) Blue silicone rubber.
Maximum temperature: 218°C (425°F).

Number of Cavities: 30
Numbering: Outside of cavities, 1-30
Block Faces: 3 x 3mm
Mold Size: 61.5 x 72 x 6mm thick

Prod # Description Unit Price Order / Quote
10586 PELCO® 3mm Square Face Mold each $14.95
Qty:
mounting cylinders Pyramid Tip Embedment, sample preparation placement on Cylinder Mount

go to Mounting Cylinders




eponate 12 kit

Eponate 12™ Embedding Kit, with DMP-30

PELCO® Technical Notes, PELCO® Eponate 12™ Kit with DMP-30 and BDMA (276KB PDF)

There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees

Prod # Description Unit Price Order / Quote
18010 Eponate 12 Kit (DMP-30 catalyst) each $381.75
Qty:
Cg, I Eponate 12™ Resin, 450g (18005) SDS (92KB PDF)
I DDSA, 450g (18022) SDS (134KB PDF)
C, I
NMA, 450g (18032) SDS (139KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
T DMP-30, 25g (18042) SDS (117KB PDF)
*NMA Hardener in increased amounts, in the embedding mixture, make a harder block.


"Spurr" Low Viscosity Kits

ERL 4221 has replaced ERL 4206. ERL 4221 has a higher viscosity than the previously used ERL 4206.

Spurr AR, 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruc Res, 26, p. 31.

Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, V 14, No 4, July 2006

The Spurr Kit gives excellent penetration for tough materials particularly in the studies of botanical, mineral and other hard biological specimens. When the mixture is kept at room temperature for 24 hours its viscosity will increase to 140cP, as is the case with other resins. When fully formulated, the Spurr’s resin will store in a deep-freeze for two months and at room temperature for two days. Curing is done at 70ºC for about 8 hours (see our Microwave system for significant time saving during epoxy processing).

PELCO® Technical Notes for Low Viscosity Kit (1.54MB PDF)

Prod # Description Unit Price Order / Quote
18300-4221 Low Viscosity "Spurr" Kit each $123.50
Qty:
Cg ERL 4221 (replaces ERL 4206), 225ml (18306-4221) SDS (95KB PDF)
I DER® 736, 250g (18310) SDS (104KB PDF)
I NSA, 450g (18301) SDS (100KB PDF)
C DMAE, 25g (18315) SDS (138KB PDF)





Conductive Adhesives for SBEM



PELCO® Conductive Liquid Silver Paint

Composition:
1-Methoxy-2 Propanol base
60% Ag
Specific Gravity = 2.25 g/cc.
Viscosity = 455 poise

PELCO® Conductive Liquid Silver Paint

a reliable conductive ground path for SEM specimens
Air drying silver paint to make effective ground. Curable at room temperature and has high adhesion to any material. Surfaces do not have to be prepared prior to application and will adhere to such materials as polymer (phenolic) boards, ceramic, glass, metal, plastic and fiberglass. It should be mixed well with a spatula before using. RoHS compliant.

The container is offered in two sizes (15g and 30g) and is provided with an applicator brush. Silver flake grain size is an average of 80%, <1.0µm. Sheet resistance is 0.02 ohms per square @ 1 mil (0.001” = 0.025mm) thickness and is a function of the coating. Service temperature range is -40°C to 200°C.
Use #16021 gold/silver thinner/extender to dilute when needed.

Technical Notes, PELCO® Colloidal Silver, Product Numbers. 16031, 16034 (417KB PDF)
F, I - 16031, 16034 SDS (153KB PDF)
F, I - 16021 SDS (PDF 147KB)

Conductive Adhesives Comparison Table

Prod # Description Unit Price Order / Quote
16031 PELCO® Colloidal Silver, 30g each $95.00
Qty:
16034 PELCO® Colloidal Silver, 15g each 49.50
Qty:
16021 PELCO® SEM-Gold/Silver Thinner/Extender, 25ml each 9.50
Qty:




silver epoxy

Silver Conductive Epoxy

Strong, highly conductive epoxy for solderless connections and repairs of circuit boards, static discharge shielding, grounding and conductive paths.

Cure Schedule (Minimum bond temperature/time)
65 to 121°C (150 to 250°F) for 5 to 10 min.
25°C (75°F) for 4 hours

Properties:

  • Bonding surface mount components
  • High strength conductive bonds
  • Quick, low or no heat cure – air dries
  • Rework or remove with hot soldering iron
  • Excellent electrical conductivity
  • 2-part epoxy, reusable applicator with stir stick, 7g each

Service temperature range -91°C to 100°C (-132°F to 212°F) permanent bond. 0.4 ohms/sq/mil (25µm)

Technical Notes, Silver Conductive Epoxy(40KB PDF)
16043 SDS Part A (119KB PDF)
I - 16043 SDS Part B (122KB PDF)

Conductive Adhesives Comparison Table

Prod # Description Unit Price Order / Quote
16043 Silver Conductive Epoxy 14g $134.25
Qty:




Sample Storage for SBEM


PELCO 18 SEM Pin Mount Storage Holder and Box

PELCO® 18 SEM Pin Mount Storage Holder and Box

Versatile, cost effective SEM pin mount storage box and holder for pin mounts with a ø3.2mm (1/8") pin. Numbered 1-18 with the spacing designed to accommodate all types of pin mounts with a diameter from 6.4 to 50mm. Can hold 18 of 12.7mm pin mounts, 8 of 18mm pin mounts, 8 of 25mm pin mounts, 2 of 32 or 38 or 50mm pin mounts with space left for the smaller pin mounts. Maximum specimen height between mount surface and closed lid is 16mm (5/8"). Used for FEI/Philips, ZEISS/LEO/Leica, Cambridge CamScan, Tescan, Amray and ETEC SEMs. Box inside dimensions are: 4-5/8" x 2-2/4" x 1-5/16" (11.7 x 7.0 x 3.3cm).

Prod # Description Unit Qty Price Order / Quote
16709 PELCO® SEM Storage box and holder for up to 18 of 12.7mm pin mounts each 1-9 $9.50
Qty:
10+ 9.10




PELCO 18 SEM Pin Mount Storage Holder and Box

PELCO® X-TREME 18 SEM Pin Mount Storage Box with Holder

Very strong and rugged clear polycarbonate box to store standard pin mounts with 3.2mm (1/8"). This box is watertight, crushproof and dustproof. The strong hinges, locking clasps and soft silicone O-ring seal makes this an ideal transport, shipping and storage box for SEM samples on pin mounts. The holder insert is numbered 1-18 and accommodates all types of pin mounts from 6.4 to 50mm diameter. Same storage capacity as #16709.

Prod # Description Unit Qty Price Order / Quote
16799 PELCO® X-TREME SEM Storage Box and holder for up to 18 of the 12.7mm SEM pin mounts each 1-4 $26.50
Qty:
5+ 23.85




PELCO Storage Box for 135 Pin Mounts

PELCO® Plastic Storage Box for 135 pin mounts

Clear styrene hinged storage box with closed cell rubber foam which holds up to 135 of ø12.7mm (1/2") pin mounts. Foam is glued to the base of the box. Complete with latched and hinged lid. There is a storage map provided with the box. Internal dimensions are 267 x 157 mm ( 10-1/2" x 6-3/16") with a maximum sample height of 24mm (15/16") on the pin stub.

Prod # Description Unit Price Order / Quote
16770 PELCO® Plastic Storage Box for 135 of 12.5 dia. pin mounts each $45.50
Qty:



PELCO® SEM Sample Stub Vacuum Desiccator 16179

PELCO® SBEM Pin Stub Vacuum Desiccator

The durable and versatile PELCO® SBEM Pin Stub Vacuum Desiccator holds up to 12 standard ø12.7mm (1/2") pin stubs, or two ø25mm (1") or 1 up to ø50mm (2") SEM pin stubs. Storage under vacuum eliminates contamination or dust. Allows for a 10mm (3/8") specimen height on the pin stubs. The use of durable, non-outgassing, blue anodized aluminum and Silicon O-rings makes it suitable for pumping down to 10-3 mbar. Capable of holding vacuum for many weeks. Metal vacuum valve with PTFE shaft seal. Use a small vacuum pump to evacuate. Vacuum connection for pump-down is a 1/4" (6.35mm) barb fitting.

  • Keeps samples and calibration standards contamination free
  • Prevents oxidization of sensitive samples
  • Holds pin mounts secure with O-rings
  • Acrylic clear disc for easy locating of samples
  • Ideal for storing, transporting and shipping samples and calibration standards

Overall size w/o vacuum valve is ø76 x 35mm (3" x 1.4"). Cavity height is 13.7mm (0.54").
Also available as a TEM Grid Vacuum Desiccator #16178 or Vacuum Desiccator for Hitachi M4 Stubs #16179-4

PELCO® Technical Notes, PELCO® SEM Pin Stub Vacuum Desiccator (103KB PDF)

Prod # Description Unit Price Order / Quote
16179 PELCO® SEM Pin Stub Vacuum Desiccator each $267.50
Qty: