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Cryo-TEM Supplies

Supplies & Consumables for the
Thermo Fisher/FEI Vitrobot System

FEI Vitrobot System

Vitrobot - Cryo Grid Boxes
Cryo Grid Boxes

NEW Twist-Top
Cryo Grid Box

NEW Bulk Storage & Hybrid Cryo Grid Boxes
Vitrobot - Cryo Grid Boxes
NEW Metal Cryo Grid
Storage Box

NEW Quick Lid

Cryo Grid Box Handling Tools
autogrid tweezers
NEW AutoGrid Tweezers
Autogrid inspection tool
NEW AutoGrid
Inspection Tool
LEICA Filter paper
Blotting Paper

NEW Foam Vitrobot
Dewar & Metal Parts
ethane condenserNEW Ethane Condenser thermo fisher fei vitrobot supplies
NEW Igloo
Vitrobot - Replacement tweezers
Tweezers Assembly for Vitrobot
Mark IV, III, II & I


Replacement Vitrobot Touch Screen Stylus
Touch Screen Stylus

Cryo Foam Dewars




cryo grid boxes for gatan 626 and ct3500 cryo transfer systems

Cryo Grid Boxes for Cryo Systems & Cryo Applications

The Cryo Grid Boxes are used for storing and transferring vitrified Cryo-EM specimens prepared with Cryo-Devices like the FEI Vitrobot. Each box holds 4 grids and is available in two colors and three versions: with clear lid, pin type lid, and without lid. All boxes have a UNC 5/40 thread in the center. On versions with clear lids, the lid is held in place with a stainless steel screw.

go to Square Cryo Grid Boxes

Prod # Description Unit Qty Price Order / Quote
160-40W Cryo Grid Box, round, White, with lid each 1-99 $11.00
Qty:
100+ 9.90
160-41W Cryo Grid Box, round, White, base only each 1-99 8.20
Qty:
100+ 6.90
160-42W Cryo Grid Box, round, White, with pin type lid each 1-99 20.40
Qty:
100+ 19.00
160-40 Cryo Grid Box, round, Blue, with lid each 1-99 11.00
Qty:
100+ 9.90
160-41 Cryo Grid Box, round, Blue, base only each 1-99 8.20
Qty:
100+ 6.90
160-42 Cryo Grid Box, round, Blue, with pin type lid each 1-99 20.40
Qty:
100+ 19.00
160-43 Replacement Clear Lid each 1-99 3.40
Qty:
100+ 2.80
160-47 Pin Type Lid, round each 1-99 13.80
Qty:
100+ 12.70




cryo grid box handling rod

NEW Twist-Top Cryo Grid Box

Similar to other Cryo Grid boxes, this grid box is used in the transfer and storage of vitrified cryo-EM samples. No special tools are required since the twist-top lid is rotated with standard laboratory tweezers. The Twist-Top Cryo Grid Box is made from a special polymer formulation for zero static at cryogenic temperatures. The box has four diamond-shaped slot positions with identification numbers and is of standard size for all common Cryo-EM sample mounting and storage devices.

Prod # Description Unit Price Order / Quote
160-58 Cryo Grid Box with Twist-Top Lid each $11.00
Qty:




cryo grid box handling rod
Hybrid Grid Box
(#160-55)

cryo grid box handling rod
Bulk Storage Grid Box
(#160-56)



NEW Bulk Storage & Hybrid Cryo Grid Boxes

These innovative grid boxes from NanoSoft eliminate several drawbacks of conventional cryo grid boxes.

The Hybrid Grid Box design is the first Cryo-EM sample storage box that has dedicated pockets for both unclipped grids and AutoGrids. This optimized design securely cradles both 4 autogrids and 4 unclipped grids while also allowing for easy placement and removal from pockets. Upon clipping, the AutoGrid can be shifted to the autogrid pocket directly across from the unclipped grid pocket, allowing for simplified vitrification workflow.

The Bulk Storage Grid Box design has a pocket with 12 slots that are optimal for cradling 12 autogrids - an entire cassette of autogrids - in one grid box. Furthermore, the slots can also hold unclipped grids securely. The first of its kind grid box can be used to store an entire 12-grid cassette of samples, from vitrification through to cassette loading, drastically improving storage density and sample traceability, and reducing storage complexity and grid box inventory requirements.

Both styles are available in 6 different colors and permanently laser-marked with serial numbering to provide easier sample traceability. These grid boxes come supplied in packs of 12 with transparent pin-type lids for easy visibility of samples. Each are compatible with all of our grid box handling tools/grippers, such as products #160-46, #160-48, #160-50.

Prod # Description Unit Price Order / Quote
160-55 Hybrid Cryo Grid Boxes with Pin Type Lids pkg/12 $165.00
Qty:
160-56 Bulk Storage Cryo Grid Boxes with Pin Type Lids pkg/12 165.00
Qty:




metal - Cryo Grid Boxes

NEW Metal Cryo Grid Storage Box

These aluminum grid boxes can be used to transfer and store up to four TEM grids during cryo-EM preparation. Most importantly, these boxes can also accommodate the autogrids from FEI/Thermo Fisher. These boxes have a notch on the side so they can be used with various cryo workstations. These grid boxes work with all of our grid box handling tools/grippers such as products #160-46, #160-48, #160-50.

Metal - Cryo Grid Boxes

Other features include:

  • Numbered wells
  • Metal pin-type lid, similar to the plastic #160-47 lids
  • Solvent resistant
  • Autoclavable
  • Easy cleaning
  • Long life
Vitrobot - Cryo Grid Boxes
Metal Cryo Grid Box held using
Cryo Grid Metal Gripper Tool, #160-50
 

Prod # Description Unit Price Order / Quote
160-52 Metal Cryo Grid Storage Box each $62.10
Qty:




cryo grid box handling rod



NEW Quick Lid

NanoSoft's Quick Lid is a simple way to protect already vitrified samples from ice particulate and other contamination that builds up in the Vitrobot dewar during sample preparation. When placed on top of a grid box, the Quick Lid acts like a shield keeping contamination out of the pockets of the grid box.

Simply cool the Quick Lid in liquid nitrogen before placing on the grid box. When a sample is ready to be placed in the grid box, place the Quick Lid in one of the empty grid box positions in the aluminum ring (keeping it under liquid nitrogen) and replace it after the grid is positioned.

The weight of the stainless steel Quick Lid keeps it in place on the grid box, reliably protecting grids from contamination. The bottom side of the lid has a small nub that easily fits into the center hole of any cryo grid box, keeping it in place. The top of the Quick Lid has a shaped pin that allows for easy transfer of the lid with the Cryo Grid Metal Gripper Tool (#160-50) or most types of tweezers.

Prod # Description Unit Price Order / Quote
160-57 Quick Lid for Cryo Grid Box each $27.00
Qty:




cryo grid box handling rod

Multicolor Cryo Grid Box Handling Rod

Now available in four different colors, the Handling Rod screws into the threaded center of #160-41 round cryo grid box for pick-up and storage applications. Can be used to store the cryo grid box in liquid nitrogen. Available in white, yellow, blue, and black.

Prod # Description Unit Price Order / Quote
160-46 Cryo Grid Box Handling Rod, White each $22.00
Qty:
160-46-4 Cryo Grid Box Handling Rod, Yellow each 22.00
Qty:
160-46-6 Cryo Grid Box Handling Rod, Blue each 22.00
Qty:
160-46-0 Cryo Grid Box Handling Rod, Black each 22.00
Qty:




gripper tool for cryo grid box

Gripper Tool for Cryo Grid Box

An ergonomic and efficient device to open the pin type lid of a cryo grid box. Spring-loaded pin vise grips the pin type lid firmly and securely. High quality aluminum body construction is good for working in liquid nitrogen. Ribbed barrel design makes it easy to unscrew the lid. This handy tool can also be used to carry the covered cryo grid box.

Prod # Description Unit Price Order / Quote
160-48 Gripper Tool for Cryo Grid Box Pin Type Lid each $39.60
Qty:




gripper tool for cryo grid box gripper tool for cryo grid box

NEW Cryo Grid Box Metal Gripper Tool

The Cryo Grid Box Metal Gripper Tool is used to handle all of our available Cryo Grid Boxes and the Quick Lid, by firmly grasping the head of the screw or the base of the pin. 

Prod # Description Unit Price Order / Quote
160-50 Stainless Steel Gripper for Cryo Grid Box each $39.60
Qty:




AutoGrid Tweezers

NEW AutoGrid Tweezers

The AutoGrid Tweezers are designed to handle the Thermo Fisher/FEI AutoGrids. The tweezer tip has a step cut out that enables the user to securely hold the AutoGrid while transferring it from the clipping station to the cassette. 
AutoGrid Tweezers

Prod # Description Unit Price Order / Quote
47000-600 AutoGrid Tweezers each $395.00
Qty:




cryo grid box handling rod


NEW AutoGrid Inspection Tool

NanoSoft's AutoGrid Inspection Tool is a low-cost way of performing a last autogrid inspection step prior to loading them into the cassette. It is placed into a grid box spot in the Thermo Fisher/FEI cassette loading station and will remain there securely due to the spring-loaded detent on the side of the tool. 

AutoGrids can be placed in the groove of the AutoGrid inspection tool and quickly flipped back and forth to check that the grid is securely clipped. It provides a final, low-cost check to help ensure only well-clipped grids are loaded into the autoloader, saving the potential disaster of a poorly clipped grid coming undone in the autoloader and weeks of downtime.

Prod # Description Unit Price Order / Quote
47000-610 AutoGrid Inspection Tool each $92.00
Qty:




LEICA Filter paper

Blotting Paper

Standard blotting filter paper for the Thermo Fisher/FEI Vitrobot and Leica Automatic Plunge Freezer.

Prod # Description Unit Price Order / Quote
47000-100 Standard Vitrobot Filter Paper, Ø55/20mm, Grade 595 pkg/100 $43.80
Qty:
47000-200 NEW Standard Leica Filter Paper, Ø55/15mm, Grade 595 pkg/100 43.80
Qty:




Vitrobot - Replacement tweezers

Tweezers Assembly for Vitrobot Mark IV, III, II & I

Complete replacement tweezers assembly for the FEI Vitrobot Mark IV-I.

Include ultra fine points DUMONT #5 tweezers with clamping block mounted on dovetail.

Prod # Description Unit Price Order / Quote
47000-500 Replacement Tweezers Assembly for FEI Vitrobot Mark IV-I each $758.50
Qty:




foam vitrobot dewar and metal parts set
metal parts set for foam vitrobot dewar

NEW Foam Vitrobot Dewar & Metal Parts Set

NanoSoft's Foam Vitrification Dewar and Metal Parts are compatible with the Vitrobot, and can also provide a robust and easy option for custom, home-made Cryo-EM sample preparation systems. Furthermore, these parts are interchangeable with FEI parts, i.e. the foam dewar works with FEI metal parts and these metal parts work with the FEI foam dewar. The foam dewar provides a well-priced, easy to acquire, robust dewar for Cryo-EM sample preparation. The design provides an improved interface, when used in conjunction with the Igloo (#47000-710) to create a better seal on the foam dewar. This combination facilitates boiled, cold nitrogen gas in the head space above the dewar, allowing for safer grid transfer to the grid box.

The Grid Box Ring (#47000-701) sits at the same height as the Vitrobot version (so grid boxes sit at same depth into dewar), but is slightly thicker and remains in contact with liquid nitrogen longer if the nitrogen level gets low. The ethane cup dimensions mimic those of the Vitrobot ethane cup, and the spider as well. These parts perform just as well or better with the Vitrobot system, but at a more affordable price than other options. 

Prod # Description Unit Price Order / Quote
47000-705 Foam Vitrification Dewar with Metal Parts Set each $1029.00
Qty:
47000-706 Foam Vitrification Dewar each 486.00
Qty:
47000-700 Metal Parts Set for Vitrification Dewar, 3 pieces each 562.50
Qty:
47000-701 Grid Box Ring each 250.00
Qty:
47000-702 Metal Spider each 215.00
Qty:
47000-703 Ethane Cup each 125.00
Qty:




cryo grid box handling rod

NEW Ethane Condenser

NanoSoft's custom designed ethane condenser tightly interfaces with Metal Spider (#47000-702) as well as the spider from the FEI/Thermo Fisher styrofoam dewar set. The user places the spider normally and inserts the condenser. The user does not need to handle an ethane nozzle and can instead focus solely on the liquid ethane level, using just one hand to control ethane flow rate, making the process much easier and ergonomic. Furthermore, the condenser should reduce wasted ethane gas and the time required to fill the cup with liquid ethane.

Prod # Description Unit Price Order / Quote
47000-709 Ethane Condenser each $134.00
Qty:




Igloo

NEW Igloo

NanoSoft’s Igloo is a nearly enclosed cover that sits atop the Vitrobot dewar, with an opening large enough for comfortable grid button access and grid transfer.  The volume enclosed by the Igloo fills with cold gaseous nitrogen, creating a dry space with improved protection versus the ambient environment. The Igloo is designed to fit comfortably over the floating styrofoam ring, minimizing the time that the styrofoam ring is exposed to ambient humidity and significantly reducing the potential for ice particulate contaminating the liquid nitrogen. The Igloo material is compatible with liquid nitrogen temperatures, allowing for easy refill with the Igloo on the dewar. Furthermore, the top surface of the Igloo remains comfortable to the touch, providing a convenient surface upon which the user can rest their hands for steady transfer.

A tweezer holder is integrated into the top surface of the Igloo providing a slot into which the Vitrobot tweezers can be placed. When resting in the slot, the tips of the tweezers remain submerged in liquid ethane. This provides freedom to the user to not have to hold the tweezers submerged in liquid ethane during transfer of the dewar, focus both hands on holding the dewar when moving it from the Vitrobot, fill the dewar with liquid nitrogen, etc.

Prod # Description Unit Price Order / Quote
47000-710 Igloo for Vitrification Dewar each $1066.00
Qty:




Replacement Vitrobot Touch Screen Stylus

Touch Screen Stylus

Overall length 100mm x Ø4.5mm at widest point, with a Ø1mm tip.

Prod # Description Unit Price Order / Quote
91000-2 Replacement Touch Screen Stylus each $11.00
Qty:




Foam Cryo Dewars

Foam Cryo Dewars

Foam dewars provide a neat and handy alternative to heavier vessels or the commonly utilized styrofoam shipping containers. The special foam material is chemically inert and mechanically durable enough to be exposed directly to liquid nitrogen, without leaking or degrading. Patented design makes the foam vessel easier to handle and safer to use than a traditional low profile glass dewar. Because of its lower thermal mass, a foam vessel will cause less liquid nitrogen boiling when it is filled. The foam surface will also accumulate less frost. As a result, less liquid nitrogen is consumed.

Available as 500ml, 800ml and 1400ml Cryo Cups featuring convenient protruding handle, a Tall (1800ml) vessel, and a Mini (130ml) vessel.

Prod # Description Unit Price Order / Quote
81760-500 Foam Cryo Cup, Small (500ml), 114mm ID x 71mm D each $99.00
Qty:
81760-800 Foam Cryo Cup, Medium (800ml), 147mm ID x 71mm D each 121.00
Qty:
81760-1400 Foam Cryo Cup, Large (1400ml), 160mm ID x 97mm D each 187.00
Qty:
81760-1800 Foam Cryo Vessel, Tall (1800ml), 89mm ID x 312mm D each 203.50
Qty:
81760-130 Foam Cryo Vessel, Mini (130ml), 51mm ID x 89mm D each 60.50
Qty: