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Histology Overview
Biological Microscopes
Calibration Overview
Light Microscopy Calibration Standards
Standard Series Stage Micrometers

Pro Series Stage Micrometers
for Transmitted and Reflected Light


Pro Stage Micrometer for Transmitted Light

The Pro Series accurate stage micrometers are intended for routine calibration of light microscopes. Manufactured with a vacuum evaporated chromium images on a glass disc. The scale is centered on the glass disc and mounted in a stainless steel slide, 75 x 24 x 2mm thick. Each slide has a unique serial number. The Pro series stage micrometers are supplied in a polished wooden box to distinguish as a traceable standard. If you don't need certification, you can also check out our Standard Series Stage Micrometers.

The Pro Series Stage Micrometers can be supplied with a choice of three certificates:

  • GRATS Traceability, Certificate of Comparison (Graticules measurement)
    This is a manufacturer calibration to provide a traceable standard. Intended for applications which do not require the accuracy provided by calibrations carried out by NPL or UKAS. Graticules provides a Certificate of Comparison for the scale which is compared with an NPL calibrated in house standard. A statement of accuracy is included. Example of Certificate of Comparison.

  • UKAS Calibration Certificate
    A UKAS accredited calibration laboratory carries out measurements at selected points on the scales and issues a calibration certificate. The UKAS certificates are internationally recognized. Example of UKAS Certificate.

  • NPL Calibration Certificate
    This is a calibration carried out by NPL (National Physics Laboratory, based in the UK). NPL carries out measurements at selected points on the scales and issues a certificate of calibration which is internationally recognize. NPL, NIST, and PTB work together on an international level and recognized each other's calibrations.
    Example of NPL Certificate.
    For the NPL certified Pro series stage micrometers we have a re-certification program available. The stage micrometers have to be in good condition to allow for re-certification. The turn-around time is 6-10 weeks.

Pro Series Stage Micrometers for Transmitted light

Pro Stage Micrometer, Transmitted
Horizontal Scale

 Stage Micrometer for transmitted light
Crossed Scale
 Pro Stage Micrometer for Transmitted Light
Mounted in a stainless steel slide

• Horizontal and crossed stage micrometers with metric or inch scales
• Horizontal scales in 10, 20, 1 or 0.1mm and 0.1 inch
• Crossed scales in 1x1mm
• Mounted in Stainless Steel, individually numbered slide
• Scales are protected with a 0.13mm thick cover glass

Table of Accuracy

Prod # Description Unit Price Order / Quote
Horizontal Scale, 10mm in 0.1mm divisions
2285-10 Micrometer Scale, 10mm in 0.1mm divisions each $321.00
Qty:
2285-10G Micrometer Scale, 10mm in 0.1mm divisions with GRATS Traceability each 704.00
Qty:
2285-10U Micrometer Scale, 10mm in 0.1mm divisions with UKAS Certificate each 733.00
Qty:
2285-10N Micrometer Scale, 10mm in 0.1mm divisions with NPL Certificate each 1456.00
Qty:
2285-10NR Recertification of NPL Certificate for Micrometer Scale, 10mm in 0.1mm divisions with NPL Certificate each 1220.00
Qty:
Horizontal Scale, 20mm in 0.01mm divisions
2285-44 Micrometer Scale, 20mm in 0.01mm divisions each 467.00
Qty:
2285-44U Micrometer Scale, 20mm in 0.01mm divisions with UKAS Certificate each 922.00
Qty:
2285-44N Micrometer Scale, 20mm in 0.01mm divisions with NPL Certificate each P.O.R.
Qty:
2285-44NR Recertification of NPL Certificate for Micrometer Scale, 20mm in 0.01mm divisions with NPL Certificate each 1315.00
Qty:
Horizontal Scale, 1mm in 0.01mm divisions
2285-13 Micrometer Scale, 1mm in 0.01mm divisions each 318.00
Qty:
2285-13G Micrometer Scale, 1mm in 0.01mm divisions with GRATS Traceability each 678.00
Qty:
2285-13U Micrometer Scale, 1mm in 0.01mm divisions with UKAS Certificate each 835.00
Qty:
2285-13N Micrometer Scale, 1mm in 0.01mm divisions with NPL Certificate each 1936.00
Qty:
2285-13NR Recertification of NPL Certificate for Micrometer Scale, 1mm in 0.01mm divisions with NPL Certificate each 1195.00
Qty:
Horizontal Scale, 0.1mm in 0.002mm divisions
2285-15 Micrometer Scale, 0.1mm in 0.002mm divisions each 463.00
Qty:
2285-15G Micrometer Scale, 0.1mm in 0.002mm divisions with GRATS Traceability each 825.00
Qty:
2285-15U Micrometer Scale, 0.1mm in 0.002mm divisions with UKAS Certificate each 1599.75
Qty:
2285-15N Micrometer Scale, 0.1mm in 0.002mm divisions with NPL Certificate each 2440.00
Qty:
2285-15NR Recertification of NPL Certificate for Micrometer Scale, 0.1mm in 0.002mm divisions with NPL Certificate each 1385.00
Qty:
Horizontal Scale, 0.1" in 0.001" divisions
2285-12 Micrometer Scale, 0.1" in 0.001" divisions each 318.00
Qty:
2285-12G Micrometer Scale, 0.1" in 0.001" divisions with GRATS Traceability each 817.00
Qty:
2285-12U Micrometer Scale, 0.1" in 0.001" divisions with UKAS Certificate each 734.00
Qty:
2285-12N Micrometer Scale, 0.1" in 0.001" divisions with NPL Certificate each 1620.00
Qty:
2285-12NR Recertification of NPL Certificate for Micrometer Scale, 0.1" in 0.001" divisions with NPL Certificate each 1229.00
Qty:
Crosssed Scales, 1mm in 0.01mm divisions
2285-16 Micrometer Crossed Scale, 1mm in 0.01mm divisions each 521.00
Qty:
2285-16G Micrometer Crossed Scale, 1mm in 0.01mm divisions with GRATS Traceability each 785.00
Qty:
2285-16U Micrometer Crossed Scale, 1mm in 0.01mm divisions with UKAS Certificate each 1225.00
Qty:
2285-16N Micrometer Crossed Scale, 1mm in 0.01mm divisions with NPL Certificate each 2459.00
Qty:
2285-16NR Recertification of NPL Certificate for Micrometer Crossed Scale, 1mm in 0.01mm divisions with NPL Certificate each 2190.00
Qty:

 

Pro Series Stage Micrometers for Reflected light

Micrometer Scale for Reflective Light
Horizontal Scale

Micrometer for Reflective Light
Mounted in a black, stainless steel slide		  

These accurate scales are etched through a highly reflective vacuum coated metal layer. When viewed with reflected (incident) light, as with a metallurgical microscope, the scales appear bright against a black background. Available with horizontal scales of 1, 10 or 20mm and 0.1 inch.

Table of accuracy

Prod # Description Unit Price Order / Quote
Horizontal Scale for Reflected Light, 10mm in 0.1mm divisions
2285-27 Micrometer Scale for Reflected Light, 10mm in 0.1mm divisions each $347.00
Qty:
2285-27G Micrometer Scale for Reflected Light, 10mm in 0.1mm divisions with GRATS Traceability each 693.00
Qty:
2285-27U Micrometer Scale for Reflected Light, 10mm in 0.1mm divisions with UKAS Certificate each 955.00
Qty:
2285-27N Micrometer Scale for Reflected Light, 10mm in 0.1mm divisions with NPL Certificate each 1663.00
Qty:
2285-27NR Recertification of NPL Certificate for Micrometer Scale for Reflected Light, 10mm in 0.1mm divisions with NPL Certificate each 1195.00
Qty:
Horizontal Scale for Reflected Light, 1mm in 0.01mm divisions
2285-101 Micrometer Scale for Reflected Light, 1mm in 0.01mm divisions each 340.00
Qty:
2285-101G Micrometer Scale for Reflected Light, 1mm in 0.01mm divisions with GRATS Traceability each 550.00
Qty:
2285-101U Micrometer Scale for Reflected Light, 1mm in 0.01mm divisions with UKAS Certificate each 836.00
Qty:
2285-101N Micrometer Scale for Reflected Light, 1mm in 0.01mm divisions with NPL Certificate each 1726.00
Qty:
2285-101NR Recertification of NPL Certificate for Micrometer Scale for Reflected Light, 1mm in 0.01mm divisions with NPL Certificate each 1175.00
Qty:
Horizontal Scale for Reflected Light, 20mm in 0.01mm divisions
2285-43 Micrometer Scale for Reflected Light, 20mm in 0.01mm divisions each 457.00
Qty:
2285-43U Micrometer Scale for Reflected Light, 20mm in 0.01mm divisions with UKAS Certificate each 986.00
Qty:
2285-43N Micrometer Scale for Reflected Light, 20mm in 0.01mm divisions with NPL Certificate each P.O.R.
Qty:
2285-43NR Recertification of NPL Certificate for Micrometer Scale for Reflected Light, 20mm in 0.01mm divisions with NPL Certificate each 1850.00
Qty:
Horizontal Scale for Reflected Light, 0.1 inch in 0.001 inch divisions
2285-28 Micrometer Scale for Reflected Light, 0.1 inch in 0.001 inch divisions each 332.00
Qty:
2285-28G Micrometer Scale for Reflected Light, 0.1 inch in 0.001 inch divisions with GRATS Traceability each 770.00
Qty:
2285-28U Micrometer Scale for Reflected Light, 0.1 inch in 0.001 inch divisions with UKAS Certificate each 799.00
Qty:
2285-28N Micrometer Scale for Reflected Light, 0.1 inch in 0.001 inch divisions with NPL Certificate each 1588.00
Qty:
2285-28NR Recertification of NPL Certificate for Micrometer Scale for Reflected Light, 0.1 inch in 0.001 inch divisions with NPL Certificate each 1195.00
Qty:

 
Line Width and Accuracy of Pro Series Stage Micrometers
Product # Line Width Accuracy (overall)
2285-10 0.005mm within 0.002 mm
2285-12 0.002mm within 0.0001 inch
2285-44 0.002mm within 0.001mm
2285-13 0.002mm within 0.001mm
2285-15 0.001 mm within 0.001mm
2285-16 0.0015mm within 0.001mm
2285-27 0.005mm within 0.002mm
2285-101 0.003mm within 0.001mm
2285-43 0.002mm within 0.001mm
2285-28 0.002mm within 0.0001 inch

 

Pro Series Dual Scale Calibration Slides for Transmitted and Reflected Light

Dual Scale Calibration Slide Dual Scale Calibration Slide - transmitted light
Dual Scale Calibration Slide for Transmitted Light 		close up Calibration Slide, Transmitted Light
enlargement 		 
Dual Scale Calibration Slide for Reflective Light Dual Scale Calibration Slide for Reflective Light
Dual Scale Calibration Slide for Reflected Light		 Dual Scale Calibration Slide for Reflective Light
enlargement		  

The dual scale calibration stage micrometers have the benefit of dual imperial/metric scales. The Pro series dual calibration scale is available for both transmitted light (black scale on glass) and reflected light (light scale on dark background). These scales are intended for calibrating optical products with a large field of view such as stereo microscopes, video microscopes and imaging systems. The scale is manufactured on a borosilicate (type B270) glass slide, 76 x 25 x1.5mm thickness with a unique serial number on each slide. Supplied in a polished wooden box.

• Horizontal Scales: 50mm in 0.1mm and 2 inch in 0.005 inch divisions
• Line thickness of 12µm
• Full glass slide; 76 x 25 x 1.5mm
• Transmitted and reflected light versions
• Individual Serial number for traceability
• Also available with UKAS Certificate

Prod # Description Unit Price Order / Quote
2285-41 Dual Scale Calibration Slide 50mm/2" for Transmitted Light each $567.00
Qty:
2285-41U Dual Scale Calibration Slide 50mm/2" for Transmitted Light with UKAS Certificate each 1799.00
Qty:
2285-45 Dual Scale Calibration Slide 50mm/2" for Reflected Light each 555.00
Qty:
2285-45U Dual Scale Calibration Slide 50mm/2" for Reflected Light with UKAS Certificate each 1151.00
Qty:

 


Pro Series of Image Analysis Standard (Reference Stage Graticule)

Reference Stage Graticule Image Analysis Standard, Reference Stage Graticule 2280-26 2285-26

  

Provides 4, high precision test areas designed for calibrating image analysis systems and identifying deviations and distortions in optical imaging systems.

The standard, which can also be used as a high precision stage micrometer, is supplied with recommendations for its use and an individual certificate of calibration.

It is produced on a 75mm x 25mm slide and has a square grid accuracy of ±0.1µm and a dot accuracy of ±0.3µm (except for the smallest and largest two dots on the root-2 array where accuracy is ±0.5µm).

The four test areas are:

  1. A 400µm x 400µm square grid which is subdivided into 200, 100, 50 and 25µm squares provides a means so detecting gross image distortions and can be used as an accurate two dimensional stage micrometer.
  2. A 20 x 17 array of nominally 15µm diameter dots can be used to identify lens distortions. i.e. to set the field of view to eliminate edge distortion.
  3. A root-2 array of spots from 3µm to 48µm diameter is used for determining the threshold level of cameras and microscope systems.
  4. A log-nominal distribution array of 100 spots ranging from 4.5µm to 27µm diameter enables the mean and standard deviation to be determined and compared with the certified values. This is an idealized distribution of maximum dynamic range for a full screen.

Supplied with a Certificate of Calibration from NPL (National Physics Laboratory, counterpart of NIST in the UK.).

Note: NPL is part of the International Metrology Group so that all of its measurements are directly traceable to the International Metre Standard held in Paris, France. This means that the certificate is internationally traceable.

Prod # Description Unit Price Order / Quote
2285-26N Image Analysis Standard Slide each $3737.00
Qty:




Pro Series Universal Calibration Slide

Dual Scale Calibration Slide Universal Calibration Slide
Universal Calibration Slide for Transmitted Light		  


The Pro Series Universal Calibration Slide combines a large number of precise images/patterns on one slide; see table for patterns. This is ideal for calibrating image analysis systems and sophisticated microscopes. Multiple images/patterns on a single slide offer quick and cost effective solution to calibrate and check the resolution of microscopes and image analysis systems. Supplied in a polished wooden box. General tolerances in microns for the universal calibration slide are:

Feature size in µm Tolerance in µm
=<10 0.5
10-50 1.0
50-127 1.3
127-250 1.9
=>250 2.54

• 14 images/patterns on one slide
• Chrome coating on glass slide
• Soda lime glass slide, 76 x 25 x 1.5mm thickness
• Individual Serial number for traceability

Prod # Description Unit Price Order / Quote
2285-42 Universal Calibration Slide with 14 images/patterns each $588.00
Qty:

ID Pattern Name Location Specification/Size
A Concentric Circles X2 / Y10 1,2,3,4,5mm circles with cross line and identifier. Line width 20µm
B Concentric Squares X10 / Y10 1,2,3,4,5 mm squares with cross line and identifier. Line width20µm
C Line Grating 25 lines/mm X18 / Y10 12.5 line pairs per mm; 40µm line 40µm space
D Line Grating 100lines/mm X26 / Y10 50 line pairs per mm; 10µm line 10µm space
E Half Protractor X34 / Y10 15 spacing. Line width 20µm
F Grid Array Coarse X40 / Y10 5mm square array with 0.5mm divisions. Central 2mm square with 0.25mm divisions. Line width 20µm
G Grid Array Fine X48 / Y10 5mm square array with 0.1mm divisions. Central 2mm square with 0.05mm divisions. Line width 8µm
H Dot Array X56 / Y10 Dot diameter 0.25mm; dot centre to centre spacing 0.50mm – 11x11grid=121 dots
I Progression of Opaque Dots X2 / Y4 Line array of dot or square shapes; clear or opaque. Reducing in size with Root 2 progression
J Progression of Opaque Squares X17 / Y4 Root 2 progression of 21 dots or squares from 3.58mm to 3.5µm
K Progression of Clear Dots X32 / Y4 Dot/Square size in mm: 3.5833; 2.5338; 1.7917; 1.2669, 0.8959; 0.6335; 0.4479; 0.3167; 0.2240
L Progression of Clear Squares X47 / Y4 0.1584;0.1120; 0.0792; 0.0560; 00396; 0.0280; 0.0198; 0.0140; 0.0099; 0.0070; 0.0049; 0.0035
M Vertical Scale Fine Variable X63 / Y2 Scale length 10mm.5mm in 0.5mm divisions; line width 20µm. 4mm in 0.1mm divisions; line width 10µm. 1mm in 0.01mm divisions; line width 3µm
N Horizontal Scale Coarse X0 / Y0 Scale length 62mm long in 2mm divisions, subdivided in 1mm. Line width 20µm

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