Calibration Overview
Standard Stage Micrometers
PRO Stage Micrometers

Light Microscopy Calibration Standards

LMS 20Magnification Calibration Standard
Pelcotec™ LMS-20G Magnification
Calibration Standard
Metric and Inch Scale Stage Micrometer for Transmitted Light
Dual, Graduated, Metric and Inch Scale Stage Micrometer for Transmitted Light
Micro-Ruler MR-1, Traceable
Micro-Ruler MR-1,

Linear Glass Scales
Sedgewick Rafter Counting Chamber, Glass
Sedgewick Rafter
Counting Chambers

Grid Counting Slide
Grid Counting Slides
England Finder
England Finder - S7 Slide
Florescence Reference Slides
Reference Slides
Standard Series Stage Micrometer
Standard Series Stage Micrometer
Pro Series Stage Micrometer
Pro Series Stage Micrometer

Pelcotec™ LMS-20G Magnification Calibration Standard

Large area, resolution 0.01mm

LMS-20G Magnification Standard
Details of the Pelcotec™ LMS-20G
The Pelcotec™ LMS-20G calibration standard for transmitted light microscopy has been designed for precise magnification calibration and microscope stage calibration. The 20 x10mm pattern is fabricated with Cr lines on clear soda lime glass. The glass is fitted in a black metal slide. Useful in the 5x to 1000x magnification range.

Specifications are:
  • Total calibration area is 20 x 10mm with crossed lines and 0.01mm divisions
  • 75nm Cr lines on clear soda lime g
  • Cross hairs showed on every 0.1mm with larger cross hairs at 0.5 and 1mm over the complete area
  • Glass thickness 550µm ± 50µm
  • Glass mounted in black metal slide, 3" x 1" x 1/16" (76.2x25.4x1.6mm)
  • Individual serial number on each Pelcotec™ LMS-20G calibration standard
  • NIST Traceable version is Pelcotec™ LMS-20GT (batch level certificate provided)
  • Individually certified version is Pelcotec™ LMS-20GC (individual certificate included)
Also available for SEM and reflected light microscopy as Pelcotec™ LMS-20 with Cr on silicon.

NIST Traceable Pelcotec™ LMS-20GT Calibration Standard
688-2 LMS-20GT Sample Certificate of Traceability (278KB PDF)

Prod # Description Unit Price Order / Quote
688-2 Pelcotec™ LMS-20GT, Magnification Calibration standard, Transmitted Light, Traceable, in 3"x 1" metal slide each $325.00
688-22 Pelcotec™ LMS-20GC, Magnification Calibration standard, Transmitted Light, Certified, in 3"x 1" metal slide each 848.50

Dual, Graduated, Metric and Inch Scale Stage Micrometer for Transmitted Light

Resolution 0.01mm/25mm length, resolution 0.001"/1" length

Diamond Ruled Stage

Bright field stage micrometer has 1" and 25mm scales, subdivided to provide calibration for various microscope magnifications.

  • Dual scale. Both metric and inch scale on same slide.
  • High definition chrome scales on soda lime glass slide (76 x 25 x 1.5mm)
  • Graduated divisions for different magnifications.
  • Metric: 25mm in 0.5mm divisions; 5mm in 0.1mm divisions; 1mm in 0.01mm divisions
  • Inch: 1" in 0.05" divisions; 0.20" in 0.01" divisions; 0.05" in 0.001" divisions
  • Foam-lined plastic case
  • Ideal for stereo microscopes, stage measurements and light microscope calibration
  • Available with UKAS (Internationally Traceable) Certificate of Calibration

Prod # Description Unit Price Order / Quote
2280-70 Dual, Graduated, Metric and English Scale Stage Micrometer each $298.00
2280-70U Dual, Graduated, Metric and English Scale Stage Micrometer, with UKAS Certificate of Calibration each 982.00

Micro-Ruler MR-1, Traceable

micro ruler MR-1

Micro-Ruler MR-1, traceable, for traceable measurements, resolution 0.01mm

The Micro-Ruler MR-1 is manufactured with anti-reflective chromium (30nm Cr2O3 over 70nm Cr) on soda-lime glass using highly accurate semiconductor manufacturing equipment. The overall scale extends over 150mm with 0.01mm increments with all labeling in mm. The ruler is designed to be viewed from either side as the markings are both right reading and mirror images.
The overall size is 25mm x 180mm x 3mm thick, the linear expansion coefficient is 9 x 10-6ppm/°C. Over its full 150mm length, the ruler will predictably change dimensions by 1.35µm/°C. The circles (diameter) and square boxes (side length) are 0.02, 0.05, 0.10, 0.50, 1.00, 2.00 and 5.00 mm. They are ideal to measure magnification simultaneously in X and Y directions or to determine image distortions such as skew, pincushion, barreling or other non-linearities.
The measurement uncertainty (accuracy) is ± 0.5µm over 0-10mm and ± 2.5µm over the entire 150mm length as measured by the National Physical Laboratory (NPL) in the UK. NPL is the NIST counterpart in the UK. The Geller MicroAnalytical Laboratory certification is made under ISO-17025 accreditation. The MR-1 is offered as a certified reference material (a traceable standard), recertification is recommended in 5 year intervals. See ordering information below for recertification service. Measurements should only by made by using pitch measurements, this is the distance between repeating parallel lines using either center-to-center or side-to-side spacing.

Prod # Description Unit Price Order / Quote
6085 Micro-ruler MR-1, NPL traceable each $1400.00
6085-70 Recertification and Clean, MR-1 each 690.00

Linear Glass Scales, resolution 0.1 or 1mm

for measurements and calibration of microscope stages and instruments

150mm Micrometer Scale linear glass scales scale magnified
#2280-35 50mm Micrometer Scale #2280-36 150mm Micrometer Scale
with storage box
#2280-36 magnified section of scale
0.1mm, 0.5mm, 1mm and 0.5cm
each cm is marked with a numeral

Hard wearing scales with vacuum deposited chrome on substantial glass substrates. Parallax free readings. Available in 50 and 150mm length. The 50mm scale is on a glass plate of 75x75x3mm and is supplied in a plastic box with a size of 165x165x25mm. Divisions are 0.1mm. The 150mm scales are available with either 0.1mm or 1mm divisions and have a glass substrate size of 180x30x6mm and are supplied in a wooden box with a size of 250x100x40mm. A UKAS certificate is optionally available for these glass scale at the time of purchase. Recertification or certification after purchase is not available.

Micrometer Scale Table of Accuracy
Prod. No. Length Divisions Line Width Accuracy
2280-35 50mm 0.1mm 0.03mm 0.002mm
2280-36 150mm 0.1mm 0.03mm 0.015mm
2280-37 150mm 1mm 0.07mm 0.01mm

Prod # Description Unit Price Order / Quote
2280-35 50mm Micrometer Scale, 0.1mm divisions each $516.00
2280-36 150mm Micrometer Scale, 0.1mm divisions each 680.00
2280-37 150mm Micrometer Scale, 1mm divisions each 660.00
For the above linear glass scales, a calibration certificate is available at the time of purchase:
2280-66 UKAS Certificate for Linear Glass Scales, Traceable to UKAS Standard each 638.00

England Finder - S7 Slide (Transmitted Light)

Locator and Calibration Slide

England Finder Slide
Progressively enlarged view of slide surface.

X (A through Z, excluding I) Y (1 through 75), note K34 to right.

England Finder Slide 2
The England Finder is a glass slide marked over the top surface in a way that a referenced position can be directly read relative to the locating edges.
All England Finders produced by Graticules for over 40 years are identical. The purpose of the finder is to give the microscopist an easy method of recording the position of a particular field of interest, so that the same position can be re-located at a later date, or by another person in another laboratory, or when using any other England Finder on any other microscope.
The location of the arrows is identical for all England Finder slides. Mark a label on the left hand side of the specimen slide, indicating the orientation to be repeated. By replacing the specimen slide with the England Finder, taking care not to disturb the position, the feature of interest can be noted. The feature an also be re-located at another place or time by reversing the procedure. Over 1,800 positions on a 76 x 26mm slide can be accurately located. Slide thickness is 1.35mm ±0.05mm. Nominal line width is 0.025mm.

Prod # Description Unit Price Order / Quote
2280-50 England Finder S7 Slide each $260.00

Sedgewick Rafter Counting Chamber

Sedgewick Rafter Counting Chamber, Plastic
Sedgewick Rafter Counting Chamber, plastic. Shown with box and coverglass.
  Sedgewick Rafter Counting Chamber, Glass
Sedgewick Rafter Counting Chamber, glass. Shown with box and coverglass.
  Sedgewick Rafter Counting Chamber, Glass
Sedgewick Rafter Counting Chamber, glass, plain without grid marking. Shown with box and coverglass

Sedgewick Rafter Counting Chambers are used in water analysis, culture inspection and applications where particles per unit volume must be ascertained. The Chamber consists of a 2mm thick clear base slide measuring 76 x 40mm. A rectangular chamber has been built onto this base. A coverglass is then placed atop the chamber.

The Chamber measures 50mm L x 20mm W x 1mm deep with a grid-marked base of 1000 1mm squares. When the chamber is filled with liquid and the coverglass in place, a 1 ml volume of liquid is trapped. Under low magnification with a light microscope, each grid square is equivalent to 1 microliter(ul) of liquid.

The S53 Sedgewick Rafter Counting Chamber is for many counting applications, APHA methods and where large particles and larger zooplankton in fluids are being analyzed. The S53 is plain glass without grid markings. The chamber holds 1 ml. of fluid. The S53 may be used with an eyepiece reticle where counting and sizing is required.

Sedgewick Rafter Chambers are available in an economical plastic version for education and training, a professional version with high quality optical glass with a chromium surface image with counting grid, and a high quality optical glass without grid markings for large particles. The coverglass is compatible with all chamber versions.

Note: Over the years the name of these slides has been incorrectly spelled as Sedgewick Rafter. The original methods were developed by William Thompson Sedgwick and George W. Rafter, so the true spelling should be Sedgwick Rafter.

Prod # Description Unit Price Order / Quote
2280-51 Sedgewick Rafter Counting Chamber, with Grid Marking, Plastic, with Coverglass each $56.00
2280-52 Coverglass for Sedgewick Rafter Counting Chamber Replacement each 18.50
2280-53 Sedgewick Rafter Counting Chamber, 1ml. volume, with Grid Marking, Glass, with Coverglass each 236.00
2280-54 Sedgewick Rafter Counting Chamber, 1ml. volume, without Grid Marking, Glass, with Coverglass each 111.10

Counting Slide

Grid Counting Slides (Transmitted Light)

Produced with precision vacuum evaporated chromium centered on a glass disc, mounted in a black anodized Al slide of 75 x 24 x 2mm. Pattern is covered with a 0.13mm cover glass for protection. Available in two sizes.

Prod # Description Unit Price Order / Quote
2280-30 Counting Slide, 0.1 x 0.1mm Grid / 1mm Square each $219.00
2280-31 Counting Slide, 0.05 x 0.05mm Grid / 0.5mm Square each 279.00

Fluorescence Reference Slide Set

Blue, Green, Yellow, and Red

Fluor-Ref Fluorescence Reference Slides Fluorescence Reference Slides enable a check of how evenly a fluorescence illuminator is filling the field of view and whether it is centered. Are the laser output and MT settings for your Confocal Microscope being monitored?

Fluorescence Reference Slides provide a quick, easy-to-use solution. Made of durable plastic, they provide a continuous fluorescent field. No hunting for microspheres. No cellular photo bleaching.

The Fluorescent Reference Set comes with four slides with spectra approximating the most commonly used fluorophores:

  • Blue excitation (DAPI / Indo / Fura)
  • Green excitation (FITC / GFP)
  • Yellow excitation (Acridine Orange)
  • Red excitation (Rhodamine / Texas Red)

To keep the slides clean, dust-free and easy to find, the set comes packaged in its own storage box.

Slide dimensions: 75 x 25 x 1.5mm., ±0.1mm for the thickness. *Please be aware there is not a specified standard variation requirement for this product.

Fluorescence Reference Slides are the innovation of Dr. P.C. Cheng of SUNY / Buffalo, NY.


Set of Four Slides

Prod # Description Unit Price Order / Quote
2273 Fluorescence Reference Slides Set of Four (Blue, Green, Yellow, and Red) each $35.80