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Neuropil of a HP frozen/freeze
substituted
hippocampus
slice culture
Daniel Studer and
Werner
Graber,
Inst. of
Anatomy,
University of Bern.
Sectioned
with the ultra sonic knife. |
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Features:
- Thinner sections
- No compression
- Best structure preservation
ultra sonic
The patented piezo actuated ultra sonic oscillating diamond knife can produce compression free, ultra-thin sections with state of the art preservation of structure.
ultra sonic Maxi
The ultra sonic Maxi oscillating diamond knife is similar to the ultra sonic but with a wider boat. It is designed specifically for biological serial sectioning.
| Knife Specifications |
ultra sonic |
ultra sonic Maxi |
| Knife Angle |
35 |
35 |
| Holder Type |
Boat |
Maxi Boat |
| Section Thickness Range (mm) |
15–100 |
15–100 |
| Blade Width (mm) |
3 |
3, 4 |
Control Unit
Frequency: 25 to 45kHz, or automatic setting of the resonance
Amplitude: variable (voltage 0 to 30V)
Mains voltage: 110V
Dimensions: 16 W x 12 D x 6cm H (6.3 x 4.72 x 2.36")
Weight: 400g (0.88 lb)
Oscillation frequency can be set between 25 and 45 kHz and amplitude is adjustable by setting the voltage between 0 and 30 volts. Using these two adjustments, the oscillation produced parallel to the cutting edge can be varied to match the cutting characteristics of the material being sectioned.
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The theoretical considerations and first results of this invention were presented in the Journal of Microscopy. Results with polymers using the oscillating knife were shown at the M&M 2002 meeting in Quebec.
The following materials were tested using the oscillating knife:
- Biological samples in Epon, Araldite, etc.
- Biological samples in acrylic resins – Lowicryl®, LR White.
- Rigid polymers such as PS, PMMA, ABS, HIPS, modified PP, etc.
Handling & Use of DiATOME® Diamond Knives (470KB PDF) |
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DiATOME® Diamond Knife Selector |
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DiATOME® Ordering Information
| Prod #
| Description
| Unit
| Price
| Order / Quote
|
| For Sale in U.S.A. Only
|
| R = Resharpen
|
| 123-US3-C3
| DiATOME® ultra sonic Diamond Knife and Control Unit, Angle 35°, Knife 3.0mm
| each
| $9950.00
|
|
| 123-US3-K3
| DiATOME® ultra sonic Diamond Knife, Angle 35°, Knife 3.0mm
| each
| 6600.00
|
|
| 123-US3-K3R
| DiATOME® ultra sonic Diamond Knife, Angle 35°, Knife 3.0mm, Resharpen
| each
| 3400.00
|
|
| 123-USM3-C3
| DiATOME® ultra sonic Maxi Diamond Knife and Control Unit, Angle 35°, Knife 3.0mm
| each
| 9950.00
|
|
| 123-USM3-K3
| DiATOME® ultra sonic Maxi Diamond Knife, Angle 35°, Knife 3.0mm
| each
| 6600.00
|
|
| 123-USM3-K3R
| DiATOME® ultra sonic Maxi Diamond Knife, Angle 35°, Knife 3.0mm, Resharpen
| each
| 3400.00
|
|
| 123-USM3-C4
| DiATOME® ultra sonic Maxi Diamond Knife and Control Unit, Angle 35°, Knife 4.0mm
| each
| 9950.00
|
|
| 123-USM3-K4
| DiATOME® ultra sonic Maxi Diamond Knife, Angle 35°, Knife 4.0mm
| each
| 4995.00
|
|
| 123-USM3-K4R
| DiATOME® ultra sonic Maxi Diamond Knife, Angle 35°, Knife 4.0mm, Resharpen
| each
| 3400.00
|
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