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DiATOME® Diamond Knives Overview

Now available from Ted Pella, Inc.

Cleaning Tools & Procedures
for DiATOME® Diamond Knives

Cleaning Procedures for DiATOME® Diamond Knives
Handling & Use of DiATOME® Diamond Knives (470KB PDF)



cleaning sticks

Diamond Knife Cleaning Rods

4 rods for cleaning the knife edge

The preferred and recommended cleaning rods for DiATOME® Diamond Knives. Shown with blade, not included.

Prod # Description Unit Price Order / Quote
For Sale in U.S.A. Only
123-2 Cleaning Rods, Box of 4 each each $14.00
Qty:




123-5

Hair Curl

Made of human hair; it is good for removing sections and cleaning of the DiATOME® Diamond Knives.

Prod # Description Unit Price Order / Quote
123-5 Hair Curl each $5.00
Qty:




123-C

Ultramicrotome Section Thickness Color Reference Chart

This reference chart allows the user to easily gauge section thickness from the interference color of cut sections. Useful for both new and experienced ultramicrotome users.

Made of durable, water resistant vinyl, this is an essential tool for any ultramicratome facility.

Prod # Description Unit Price Order / Quote
123-C Ultramicrotome Section Thickness Color Reference Chart each $11.50
Qty:




DiATOME® Cleaning Procedure for Diamond Knives

This procedure serves for the cleaning of DiATOME® Diamond Knives. We are at your disposal for any further assistance you might require.

   
1. For cryo knives only: Remove the knife stage from the cryo chamber (before heating the chamber up), rinse under tap water to warm it up.   2. Bevel a polystyrene rod with a clean, degreased blade. Dip into 50% ethanol for cryo knives or 70–80% ethanol for standard knives.   3. Pass the polystyrene rod over the cutting edge without applying pressure.
         
     
4. Rinse with 50% ethanol. (Non-cryo knives can be rinsed with up to 80% ethanol.)   5. Dry the knife with a dust blower or canned air.    


* References:

Liou, Willisa, Hans J. Geuze, and Jan W. Slot. "Improving structural integrity of cryosections for immunogold labeling." Histochemistry and cell biology 106 (1996): 41-58.

Peters, Peter J., Erik Bos, and Alexander Griekspoor. "Cryo‐immunogold electron microscopy." Current protocols in cell biology 30.1 (2006): 4-7.