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The first cryo knife with a diamond platform, guarantees the best possible sectioning for sucrose infiltrated samples (Tokuyasu).
The diamond platform provides for easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet.
The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples.
Liou, Willisa, Hans J. Geuze, and Jan W. Slot. "Improving structural integrity of cryosections for immunogold labeling." Histochemistry and cell biology 106 (1996): 41-58. Peters, Peter J., Erik Bos, and Alexander Griekspoor. "Cryo‐immunogold electron microscopy." Current protocols in cell biology 30.1 (2006): 4-7.