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DiATOME® Diamond Knives Overview
Cleaning Tools for DiATOME® Diamond Knives

Now available from Ted Pella, Inc.

DiATOME® cryo immuno
Diamond Knives

Available for sale in U.S.A. only


The first cryo knife with a diamond platform, guarantees the best possible sectioning for sucrose infiltrated samples (Tokuyasu).

The diamond platform provides for easy and gentle section collection. Sections are collected directly from the diamond surface using a loop and a sucrose/methylcellulose droplet.

The 35° cutting angle leads to a considerable reduction in mechanical stresses and therefore to improved structure preservation in sucrose-protected samples. 

Knife Specifications

Knife angle: 35°
Thickness range: 30-300nm
Available sizes: 2mm, 3mm
Cleaning Procedure for DiATOME® cryo immuno Diamond Knives
Handling & Use of DiATOME® Diamond Knives (860KB PDF)


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Price: P.O.R Qty:


DiATOME® Ordering Information

Prod # Description Unit Price Order / Quote
For Sale in U.S.A. Only
R = Resharpen
123-DK3-CI20 DiATOME® cryo immuno Diamond Knife, Angle 35°, Knife 2.0mm each P.O.R.
Qty:
123-DK3-CI20R DiATOME® cryo immuno Diamond Knife, Angle 35°, Knife 2.0mm, Resharpen each 1800.00
Qty:
123-DK3-CI30 DiATOME® cryo immuno Diamond Knife, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CI30R DiATOME® cryo immuno Diamond Knife, Angle 35°, Knife 3.0mm, Resharpen each 2595.00
Qty:




DiATOME® Cleaning Procedure for cryo immuno Diamond Knives

This procedure serves for the cleaning of our cryo immuno knives. We are at your disposal for any further assistance you might require.

1. Remove the knife stage from the cryo
chamber (before heating the chamber up),
rinse under tap water to warm it up.		 2. Bevel a polystyrene stick with a clean,
degreased blade. Dip into 50% ethanol.		 3. Pass the polystyrene rod over the cutting
edge without applying pressure.
     
 
4. Rinse with 50-80% ethanol. 5. Dry the knife with a dust blower or canned air.  


References
* Liou, Willisa, Hans J. Geuze, and Jan W. Slot. "Improving structural integrity of cryosections for immunogold labeling." Histochemistry and cell biology 106 (1996): 41-58.
* Peters, Peter J., Erik Bos, and Alexander Griekspoor. "Cryo‐immunogold electron microscopy." Current protocols in cell biology 30.1 (2006): 4-7.