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DiATOME® Diamond Knives Overview
Cryo-EM Supplies
Cleaning Tools & Procedures for DiATOME® Diamond Knives

Now available from Ted Pella, Inc.

DiATOME® cryo Diamond Knives

Diamond knives for low temperature EM and LM applications

Available for sale in U.S.A. only


cryo 35°

The cryo 35° knife has demonstrated its usefulness as a standard knife for the low temperature sectioning of polymers, rubber, paints, etc. The 35° angle represents a balanced compromise between section quality and durability. Two styles (wet and dry) are available.

cryo 45°

The cryo 45° knife is well suited for routine cryo sectioning. Like the cryo 35° it is available in two styles (wet and dry).
   
Knife Specifications cryo (dry) cryo (wet)
Knife Angle 35, 45 35, 45
Holder Type Triangular Boat
Section Thickness Range (mm) 30–300 30–300
Blade Width (mm) 1.5, 2, 2.5, 3, 3.5, 4 1.5, 2, 2.5, 3, 3.5, 4


Handling & Use of DiATOME® Diamond Knives (860KB PDF)

product selector icon DiATOME® Diamond Knife Selector
Knife Type & Angle Width Select
Price: $1800.00 Qty:


DiATOME® Ordering Information

Prod # Description Unit Price Order / Quote
Cryo Dry, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK3-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm each $2500.00
Qty:
123-DK3-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK3-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK3-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm each 2895.00
Qty:
123-DK3-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm, Exchange each 2200.00
Qty:
123-DK3-CD20R DiATOME® cryo Diamond Knife, Dry Angle 35°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK3-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm each 3500.00
Qty:
123-DK3-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK3-CD25R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK3-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm each 4200.00
Qty:
123-DK3-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK3-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK3-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm each 4650.00
Qty:
123-DK3-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Exchange each 3400.00
Qty:
123-DK3-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Resharpen each 3000.00
Qty:
123-DK3-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm each 4995.00
Qty:
123-DK3-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Exchange each 3750.00
Qty:
123-DK3-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Resharpen each 3300.00
Qty:
123-DK4-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm each 2500.00
Qty:
123-DK4-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK4-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK4-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm each 2700.00
Qty:
123-DK4-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK4-CD20R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK4-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm each 3500.00
Qty:
123-DK4-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK4-CD25R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK4-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm each 4200.00
Qty:
123-DK4-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK4-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK4-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm each 4650.00
Qty:
123-DK4-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Exchange each 3400.00
Qty:
123-DK4-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Resharpen each 3000.00
Qty:
123-DK4-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm each 4995.00
Qty:
123-DK4-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Exchange each 3750.00
Qty:
123-DK4-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Resharpen each 3300.00
Qty:

Prod # Description Unit Price Order / Quote
Cryo Wet, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK3-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm each $2500.00
Qty:
123-DK3-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK3-CW15R DiATOME® Cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK3-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm each 3100.00
Qty:
123-DK3-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Exchange each 2200.00
Qty:
123-DK3-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Resharpen each 1825.00
Qty:
123-DK3-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm each 3500.00
Qty:
123-DK3-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK3-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK3-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm each 4200.00
Qty:
123-DK3-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK3-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK3-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm each 4650.00
Qty:
123-DK3-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Exchange each 3400.00
Qty:
123-DK3-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Resharpen each 3000.00
Qty:
123-DK3-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm each 4995.00
Qty:
123-DK3-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Exchange each 3750.00
Qty:
123-DK3-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Resharpen each 3300.00
Qty:
123-DK4-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm each 2500.00
Qty:
123-DK4-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK4-CW15R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK4-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm each 3100.00
Qty:
123-DK4-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Exchange each 2200.00
Qty:
123-DK4-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Resharpen each 1825.00
Qty:
123-DK4-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm each 3500.00
Qty:
123-DK4-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK4-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK4-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm each 4200.00
Qty:
123-DK4-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK4-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK4-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm each 4650.00
Qty:
123-DK4-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Exchange each 3400.00
Qty:
123-DK4-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Resharpen each 3000.00
Qty:
123-DK4-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm each 4995.00
Qty:
123-DK4-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Exchange each 3700.00
Qty:
123-DK4-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Resharpen each 3300.00
Qty:

product selector icon Use the DiATOME® Diamond Knife Selector Tool


*References:

Al-Amoudi, Ashraf, Daniel Studer, and Jacques Dubochet. "Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy." Journal of structural biology 150.1 (2005): 109-121.

Michel, M., H. Gnägi, and M. Müller. "Diamonds are a cryosectioner's best friend." Journal of Microscopy 166.1 (1992): 43-56.

Richter, Karsten. "Cutting artefacts on ultrathin cryosections of biological bulk specimens." Micron 25.4 (1994): 297-308.

Zhang, P., et al. "Direct visualization of receptor arrays in frozen‐hydrated sections and plunge‐frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr." Journal of microscopy 216.1 (2004): 76-83.