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DiATOME® Diamond Knives Overview
Cleaning Tools for DiATOME® Diamond Knives

Now available from Ted Pella, Inc.

DiATOME® cryo Diamond Knives

Diamond knives for low temperature EM and LM applications

Available for sale in U.S.A. only


cryo 35°

The cryo 35° knife has demonstrated its usefulness as a standard knife for the low temperature sectioning of polymers, rubber, paints, etc. The 35° angle represents a balanced compromise between section quality and durability. Two styles (wet and dry) are available. Mounted in the triangular holder cryo 35° is suitable for dry cryo sectioning. The 35° knife mounted in the trough are used for sectioning with fluids such as a DMSO/water mixture. Section thicknesses range 30 – 300nm. Triangular holder (dry) or small boat (wet). Blade widths 1.5 to 4.0mm.

cryo 45°

The cryo 45° knife is well suited for routine cryo sectioning. Like the cryo 35° it is available in two styles (wet and dry). Mounted in the triangular holder cryo 45° is suitable for dry cryo sectioning. The 45° knife mounted in the trough are used for sectioning with fluids such as a DMSO/water mixture. Section thicknesses range 30 to 300nm. Triangular holder (dry) or small boat (wet). Blade widths 1.5 to 4.0mm.
Handling & Use of DiATOME® Diamond Knives (860KB PDF)


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Knife Type & Angle Width Select
Price: $1800.00 Qty:


DiATOME® Ordering Information

Prod # Description Unit Price Order / Quote
Cryo Dry, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK3-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm each $2500.00
Qty:
123-DK3-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK3-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK3-CD18R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.8mm, Resharpen each 1750.00
Qty:
123-DK3-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm each 2895.00
Qty:
123-DK3-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm, Exchange each 2200.00
Qty:
123-DK3-CD20R DiATOME® cryo Diamond Knife, Dry Angle 35°,, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK3-CD21R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.1mm, Resharpen each 1825.00
Qty:
123-DK3-CD24R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.4mm, Resharpen each 2075.00
Qty:
123-DK3-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm each P.O.R.
Qty:
123-DK3-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK3-CD25R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK3-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK3-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK3-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm each P.O.R.
Qty:
123-DK3-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK3-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Resharpen each P.O.R.
Qty:
123-DK3-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm each P.O.R.
Qty:
123-DK3-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK3-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Resharpen each P.O.R.
Qty:
123-DK4-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm each 2500.00
Qty:
123-DK4-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK4-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK4-CD18R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.8mm, Resharpen each 1750.00
Qty:
123-DK4-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm each 2700.00
Qty:
123-DK4-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK4-CD20R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK4-CD21R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.1mm, Resharpen each 1825.00
Qty:
123-DK4-CD24R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.4mm, Resharpen each 2075.00
Qty:
123-DK4-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm each P.O.R.
Qty:
123-DK4-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK4-CD25R DiATOME® cryo Diamond Knife, Dry, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK4-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm each P.O.R.
Qty:
123-DK4-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK4-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK4-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm each P.O.R.
Qty:
123-DK4-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK4-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Resharpen each P.O.R.
Qty:
123-DK4-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm each P.O.R.
Qty:
123-DK4-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK4-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Resharpen each P.O.R.
Qty:

Prod # Description Unit Price Order / Quote
Cryo Wet, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK3-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm each $2500.00
Qty:
123-DK3-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK3-CW15R DiATOME® Cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK3-CW18R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.8mm, Resharpen each 1750.00
Qty:
123-DK3-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm each P.O.R.
Qty:
123-DK3-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Exchange each 2200.00
Qty:
123-DK3-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Resharpen each 1825.00
Qty:
123-DK3-CW21R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.1mm, Resharpen each 1825.00
Qty:
123-DK3-CW24R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.4mm, Resharpen each 2075.00
Qty:
123-DK3-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm each P.O.R.
Qty:
123-DK3-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK3-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK3-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK3-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK3-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm each P.O.R.
Qty:
123-DK3-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK3-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Resharpen each P.O.R.
Qty:
123-DK3-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm each P.O.R.
Qty:
123-DK3-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK3-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Resharpen each P.O.R.
Qty:
123-DK4-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm each 2500.00
Qty:
123-DK4-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Exchange each 1800.00
Qty:
123-DK4-CW15R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Resharpen each 1225.00
Qty:
123-DK4-CW18R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.8mm, Resharpen each 1750.00
Qty:
123-DK4-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm each P.O.R.
Qty:
123-DK4-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Exchange each 2200.00
Qty:
123-DK4-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Resharpen each 1825.00
Qty:
123-DK4-CW21R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.1mm, Resharpen each 1825.00
Qty:
123-DK4-CW24R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.4mm, Resharpen each 2075.00
Qty:
123-DK4-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm each P.O.R.
Qty:
123-DK4-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Exchange each 2500.00
Qty:
123-DK4-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Resharpen each 2075.00
Qty:
123-DK4-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm each P.O.R.
Qty:
123-DK4-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Exchange each 2995.00
Qty:
123-DK4-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Resharpen each 2595.00
Qty:
123-DK4-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm each P.O.R.
Qty:
123-DK4-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK4-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Resharpen each P.O.R.
Qty:
123-DK4-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm each P.O.R.
Qty:
123-DK4-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK4-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Resharpen each P.O.R.
Qty:

Use the DiATOME® Diamond Knife Selector Tool


References
* Al-Amoudi, Ashraf, Daniel Studer, and Jacques Dubochet. "Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy." Journal of structural biology 150.1 (2005): 109-121.
* Michel, M., H. Gnägi, and M. Müller. "Diamonds are a cryosectioner's best friend." Journal of Microscopy 166.1 (1992): 43-56.
* Richter, Karsten. "Cutting artefacts on ultrathin cryosections of biological bulk specimens." Micron 25.4 (1994): 297-308.
* Zhang, P., et al. "Direct visualization of receptor arrays in frozen‐hydrated sections and plunge‐frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr." Journal of microscopy 216.1 (2004): 76-83.