We use cookies necessary for the functioning and optimization of our website. We do not retain any personal data once the session has ended except that which is necessary to process an order that you have placed through the website.

For more information, please read our Privacy Policy.
 in 

DiATOME® Diamond Knives Overview
Cleaning Tools for DiATOME® Diamond Knives

Now available from Ted Pella, Inc.

DiATOME® cryo Diamond Knives

Diamond knives for low temperature EM and LM applications

Available for sale in U.S.A. only


cryo 25°

The 25° angle of the cryo 25° diamond knife produces sections of frozen hydrated specimens with the least compression and the best structural preservation possible. It is recommended that sectioning be done in a low humidity glove box and the sections should be attached by electrostatic force. Section thicknesses range from 30 to 150nm. Triangular holder. Blade width, 3.0mm.

cryo 35°

The cryo 35° knife has demonstrated its usefulness as a standard knife for the low temperature sectioning of polymers, rubber, paints, etc. The 35° angle represents a balanced compromise between section quality and durability. Two styles (wet and dry) are available. Mounted in the triangular holder cryo 35° is suitable for dry cryo sectioning. The 35° knife mounted in the trough are used for sectioning with fluids such as a DMSO/water mixture. Section thicknesses range 30 – 300nm. Triangular holder (dry) or small boat (wet). Blade widths 1.5 to 4.0mm.

cryo 45°

The cryo 45° knife is well suited for routine cryo sectioning. Like the cryo 35° it is available in two styles (wet and dry). Mounted in the triangular holder cryo 45° is suitable for dry cryo sectioning. The 45° knife mounted in the trough are used for sectioning with fluids such as a DMSO/water mixture. Section thicknesses range 30 to 300nm. Triangular holder (dry) or small boat (wet). Blade widths 1.5 to 4.0mm.
Cleaning Procedure for DiATOME® cryo Diamond Knives
Handling & Use of DiATOME® Diamond Knives (500KB PDF)


product selector icon DiATOME® Diamond Knife Selector
Knife Type & Angle Width Select
Price: $1650.00 Qty:


DiATOME® Ordering Information

Prod # Description Unit Price Order / Quote
Cryo Dry, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK2-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 25°, Knife 3.0mm each P.O.R.
Qty:
123-DK2-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 25°, Knife 3.0mm, Resharpen each 2900.00
Qty:
123-DK3-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm each 2350.00
Qty:
123-DK3-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Exchange each 1650.00
Qty:
123-DK3-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.5mm, Resharpen each 1199.00
Qty:
123-DK3-CD18R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 1.8mm, Resharpen each 1650.00
Qty:
123-DK3-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm each 2895.00
Qty:
123-DK3-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.0mm, Exchange each 2000.00
Qty:
123-DK3-CD20R DiATOME® cryo Diamond Knife, Dry Angle 35°,, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK3-CD21R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.1mm, Resharpen each 1700.00
Qty:
123-DK3-CD24R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.4mm, Resharpen each 1950.00
Qty:
123-DK3-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm each P.O.R.
Qty:
123-DK3-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Exchange each 2175.00
Qty:
123-DK3-CD25R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK3-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Exchange each 2775.00
Qty:
123-DK3-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.0mm, Resharpen each 2395.00
Qty:
123-DK3-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm each P.O.R.
Qty:
123-DK3-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK3-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 3.5mm, Resharpen each 2775.00
Qty:
123-DK3-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm each P.O.R.
Qty:
123-DK3-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK3-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 35°, Knife 4.0mm, Resharpen each P.O.R.
Qty:
123-DK4-CD15 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm each 2350.00
Qty:
123-DK4-CD15E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Exchange each 1650.00
Qty:
123-DK4-CD15R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.5mm, Resharpen each 1199.00
Qty:
123-DK4-CD18R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 1.8mm, Resharpen each 1650.00
Qty:
123-DK4-CD20 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm each 2700.00
Qty:
123-DK4-CD20E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK4-CD20R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK4-CD21R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.1mm, Resharpen each 1700.00
Qty:
123-DK4-CD24R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.4mm, Resharpen each 1950.00
Qty:
123-DK4-CD25 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm each P.O.R.
Qty:
123-DK4-CD25E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 2.5mm, Exchange each 2175.00
Qty:
123-DK4-CD25R DiATOME® cryo Diamond Knife, Dry, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK4-CD30 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm each P.O.R.
Qty:
123-DK4-CD30E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Exchange each 2775.00
Qty:
123-DK4-CD30R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.0mm, Resharpen each 2395.00
Qty:
123-DK4-CD35 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm each P.O.R.
Qty:
123-DK4-CD35E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK4-CD35R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 3.5mm, Resharpen each 2775.00
Qty:
123-DK4-CD40 DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm each P.O.R.
Qty:
123-DK4-CD40E DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK4-CD40R DiATOME® cryo Diamond Knife, Dry, Angle 45°, Knife 4.0mm, Resharpen each P.O.R.
Qty:

Prod # Description Unit Price Order / Quote
Cryo Wet, For Sale in U.S.A. Only
R = Resharpen, E = Exchange
123-DK3-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm each $2350.00
Qty:
123-DK3-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Exchange each 1650.00
Qty:
123-DK3-CW15R DiATOME® Cryo Diamond Knife, Wet, Angle 35°, Knife 1.5mm, Resharpen each 1199.00
Qty:
123-DK3-CW18R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 1.8mm, Resharpen each 1650.00
Qty:
123-DK3-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm each 2418.00
Qty:
123-DK3-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK3-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK3-CW21R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.1mm, Resharpen each 1700.00
Qty:
123-DK3-CW24R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.4mm, Resharpen each 2895.00
Qty:
123-DK3-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm each P.O.R.
Qty:
123-DK3-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Exchange each 2300.00
Qty:
123-DK3-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK3-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Exchange each 2775.00
Qty:
123-DK3-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.0mm, Resharpen each 2395.00
Qty:
123-DK3-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm each P.O.R.
Qty:
123-DK3-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK3-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 3.5mm, Resharpen each 2775.00
Qty:
123-DK3-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm each P.O.R.
Qty:
123-DK3-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK3-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 35°, Knife 4.0mm, Resharpen each P.O.R.
Qty:
123-DK4-CW15 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm each 2350.00
Qty:
123-DK4-CW15E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Exchange each 1650.00
Qty:
123-DK4-CW15R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.5mm, Resharpen each 1199.00
Qty:
123-DK4-CW18R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 1.8mm, Resharpen each 1650.00
Qty:
123-DK4-CW20 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm each 2700.00
Qty:
123-DK4-CW20E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Exchange each 1695.00
Qty:
123-DK4-CW20R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.0mm, Resharpen each 1390.00
Qty:
123-DK4-CW21R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.1mm, Resharpen each 1700.00
Qty:
123-DK4-CW24R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.4mm, Resharpen each 1950.00
Qty:
123-DK4-CW25 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm each P.O.R.
Qty:
123-DK4-CW25E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Exchange each 2175.00
Qty:
123-DK4-CW25R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 2.5mm, Resharpen each 1900.00
Qty:
123-DK4-CW30 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm each P.O.R.
Qty:
123-DK4-CW30E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Exchange each 2775.00
Qty:
123-DK4-CW30R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.0mm, Resharpen each 2395.00
Qty:
123-DK4-CW35 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm each P.O.R.
Qty:
123-DK4-CW35E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Exchange each P.O.R.
Qty:
123-DK4-CW35R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 3.5mm, Resharpen each 2775.00
Qty:
123-DK4-CW40 DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm each P.O.R.
Qty:
123-DK4-CW40E DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Exchange each P.O.R.
Qty:
123-DK4-CW40R DiATOME® cryo Diamond Knife, Wet, Angle 45°, Knife 4.0mm, Resharpen each P.O.R.
Qty:

Use the DiATOME® Diamond Knife Selector Tool




DiATOME® Cleaning Procedure for Cryo Knives

This procedure serves for the cleaning of our cryo immuno and cryo 25° knives. We are at your disposal for any further assistance you might require.

1. Remove the knife stage from the cryo
chamber, leave the knives in the stage,
wash under tap water for warming up.		 2. Bevel a polystyrene stick with a
perfectly clean razor blade.		 3. Clean the knife with 50% ethanol,
keep wet.
     
 
4. Rinse with 100% ethanol. 5. Dry with a dustblower or canned air.  


References
*A. Al-Amoudi, D. Studer and J. Dubochet: Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. Journal of Structural Biology 150, pp. 109-121, 2005.
*W. Liu, H. J. Geuze, J. W. Slot: Improving structural integrity of cryosections for immunogold labeling Histochemistry and Cell Biohgy, Vol. 106, PP. 41-55, 1996.
*M. Michel, H. Gnagi and M. Muller: Diamonds are a cryosectioner's best friend. Journal of Microscopy, Vol 166, Pt 1, 43-56, 1992.
*P. J. Peters: Cryo-lmmunogold Electron Microscopy, In Current Protocols in Cell Biology {J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz and K.M. Yamada, eds) pp. 4.7.1-4.7.12, 1999. Jonn Wiley & Sons, New York.
*K. Richter: Cutting artifacts on ultrathin cryosections of biological bulk specimens. Micron, Vol. 25, No. 4, pp. 297-308. 1994.
*P. Zhang, E. Bos, J. Heymann, H. Gnaegi, M. Kessel, P.J. Peters, S. Subramaniam: Direct visualisation of receptor arrays in frozen-hydrated sections and plungefrozen specimens of E.coli engineered to overproduce the chemotaxis receptor Tsr. Journal of Microscopy, Vol. 216, Pt 1, pp. 76-83, 2004.