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DiATOME® Diamond Knives Overview
Cryo-EM Supplies
Cleaning Tools & Procedures for DiATOME® Diamond Knives

Now available from Ted Pella, Inc.

DiATOME® Diamond Knives for CEMOVIS

(Cryo-Electron Microscopy Of Vitreous Sections)

Available for sale in U.S.A. only



cemovis diamond knives by diatome

The CEMOVIS 35° knife and the CEMOVIS 25° knife are designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.

Please note: best results are achieved at low humidity, when the cryo-ultramicrotome is placed in a glovebox and the sections attached by electrostatic force.

Knife Specifications CEMOVIS
Knife Angle 25, 35
Holder Type Triangular
Section Thickness Range (mm) 30–150
Blade Width (mm) 3

Handling & Use of DiATOME® Diamond Knives (470KB PDF)


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DiATOME® Ordering Information

Prod # Description Unit Price Order / Quote
For Sale in U.S.A. Only
R = Resharpen
123-DK2-CEM30 DiATOME® CEMOVIS Diamond Knife, Angle 25°, Knife 3.0mm each $4200.00
Qty:
123-DK2-CEM30R DiATOME® CEMOVIS Diamond Knife, Angle 25°, Knife 3.0mm, Resharpen each 2995.00
Qty:
123-DK3-CEM30 DiATOME® CEMOVIS Diamond Knife, Angle 35°, Knife 3.0mm each 4200.00
Qty:
123-DK3-CEM30R DiATOME® CEMOVIS Diamond Knife, Angle 35°, Knife 3.0mm, Resharpen each 2995.00
Qty:


*References:

Pierson, Jason, et al. "Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box." Journal of structural biology 169.2 (2010): 219-225.

Han, H‐M., Benoît Zuber, and J. Dubochet. "Compression and crevasses in vitreous sections under different cutting conditions." Journal of microscopy 230.2 (2008): 167-171.

Al-Amoudi, Ashraf, Daniel Studer, and Jacques Dubochet. "Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy." Journal of structural biology 150.1 (2005): 109-121.

Michel, M., H. Gnägi, and M. Müller. "Diamonds are a cryosectioner's best friend." Journal of Microscopy 166.1 (1992): 43-56.
Richter, Karsten. "Cutting artefacts on ultrathin cryosections of biological bulk specimens." Micron 25.4 (1994): 297-308.

Zhang, P., et al. "Direct visualization of receptor arrays in frozen‐hydrated sections and plunge‐frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr." Journal of microscopy 216.1 (2004): 76-83.