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DiATOME® Diamond Knives Overview
Cleaning Tools for DiATOME® Diamond Knives

Now available from Ted Pella, Inc.

DiATOME® Diamond Knives for CEMOVIS

(Cryo-Electron Microscopy Of Vitreous Sections)

Available for sale in U.S.A. only



cemovis diamond knives by diatome

The CEMOVIS 35° knife and the CEMOVIS 25° knife are designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.

Please note: best results are achieved at low humidity, when the cryo-ultramicrotome is placed in a glovebox and the sections attached by electrostatic force.

Knife Specifications

Knife angles: 25°, 35°
Thickness range: 30-150nm
Available size: 3mm

Cleaning Procedure for DiATOME® CEMOVIS Diamond Knives
Handling & Use of DiATOME® Diamond Knives (860KB PDF)


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Price: P.O.R Qty:


DiATOME® Ordering Information

Prod # Description Unit Price Order / Quote
For Sale in U.S.A. Only
R = Resharpen
123-DK2-CEM30 DiATOME® CEMOVIS Diamond Knife, Angle 25°, Knife 3.0mm each P.O.R.
Qty:
123-DK2-CEM30R DiATOME® CEMOVIS Diamond Knife, Angle 25°, Knife 3.0mm, Resharpen each 2995.00
Qty:
123-DK3-CEM30 DiATOME® CEMOVIS Diamond Knife, Angle 35°, Knife 3.0mm each P.O.R.
Qty:
123-DK3-CEM30R DiATOME® CEMOVIS Diamond Knife, Angle 35°, Knife 3.0mm, Resharpen each 2995.00
Qty:



DiATOME® Cleaning Procedure for CEMOVIS Diamond Knives

This procedure serves for the cleaning of our DiATOME® CEMOVIS knives. We are at your disposal for any further assistance you might require.

1. Remove the knife stage from the cryo
chamber (before heating the chamber up), rinse under tap water to warm it up.		 2. Bevel a polystyrene stick with a clean, degreased blade. Dip into 50% ethanol. 3. Pass the polystyrene rod over the cutting edge without applying pressure.
     
 
4. Rinse with 50-80% ethanol. 5. Dry the knife with a dust blower or canned air.  


References
* Pierson, Jason, et al. "Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box." Journal of structural biology 169.2 (2010): 219-225.
* Han, H‐M., Benoît Zuber, and J. Dubochet. "Compression and crevasses in vitreous sections under different cutting conditions." Journal of microscopy 230.2 (2008): 167-171.
* Al-Amoudi, Ashraf, Daniel Studer, and Jacques Dubochet. "Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy." Journal of structural biology 150.1 (2005): 109-121.
* Michel, M., H. Gnägi, and M. Müller. "Diamonds are a cryosectioner's best friend." Journal of Microscopy 166.1 (1992): 43-56.
* Richter, Karsten. "Cutting artefacts on ultrathin cryosections of biological bulk specimens." Micron 25.4 (1994): 297-308.
* Zhang, P., et al. "Direct visualization of receptor arrays in frozen‐hydrated sections and plunge‐frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr." Journal of microscopy 216.1 (2004): 76-83.