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Cryo-EM Supplies | Cryo-EM Tweezers
Cryo Safety Supplies | Electron Microscopy Embedding

High Pressure Freezing


cryo freezer hats
PELCO® Freezer Hats
NEW styles available!		 sapphire discs
NEW PELCO®
Sapphire Discs		 cryo freezer hats
NEW HPF Hat Storage Boxes		 18037
NEW 1-Hexadecene


PELCO® Freezer Hats for High Pressure Freezing

NEW Styles Available!

cryo freezer hats
Freezer Hats are used in High Pressure Freezers such as:
  • Leica
  • Wohlwend
  • CryoCapCell
  • ABRA
  • RMC Boeckeler
  • Balzers
  • Bal-Tec
 cryo freezer hats cryo freezer hats
Conveniently store freezer hats in the HPF Hat Storage Boxes.
Planchet designs are roughly shown below in cross section. They are available in brass only. Depths of cavity in the planchets are 0.05, 0.1, 0.15, 0.2, 0.25 and 0.3mm. Available in both 3mm and 6mm diameter.

Stacking two plachets, in various combinations, creates a cavity ranging from 0.05mm to 0.6mm. Please refer to the following technical note: Technical Notes, PELCO® Freezer Hats (739KB PDF)

planchet a

Brass Planchet 0.1/0.2mm		 planchet b

Brass Planchet 0.0/0.3mm


planchet a

Brass Planchet 0.15/0.15mm

planchet b

Brass Planchet 0.05/0.25mm

Prod # Description Unit Price Order / Quote
3mm Specimen Holders
39200 PELCO® Freezer Hats, Brass, Specimen Holders, 0.1/0.2mm, Ø3mm pkg/50 $299.00
Qty:
39201 PELCO® Freezer Hats, Brass, Specimen Holders, 0.0/0.3mm, Ø3mm pkg/50 299.00
Qty:
39204 NEW PELCO® Freezer Hats, Brass, Specimen Holders, 0.05/0.25mm, Ø3mm pkg/50 299.00
Qty:
39206 NEW PELCO® Freezer Hats, Brass, Specimen Holders, 0.15/0.15mm, Ø3mm pkg/50 299.00
Qty:
6mm Specimen Holders
39202 PELCO® Freezer Hats, Brass, Specimen Holders, 0.1/0.2mm, Ø6mm pkg/50 299.00
Qty:
39203 PELCO® Freezer Hats, Brass, Specimen Holders, 0.0/0.3mm, Ø6mm pkg/50 299.00
Qty:
39205 NEW PELCO® Freezer Hats, Brass, Specimen Holders, 0.05/0.25mm, Ø6mm pkg/50 299.00
Qty:
39207 NEW PELCO® Freezer Hats, Brass, Specimen Holders, 0.15/0.15mm, Ø6mm pkg/50 299.00
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sapphire discs

NEW PELCO® Sapphire Discs

The PELCO® Sapphire Discs are made from high purity clear sapphire (Al2O3), ground and optically clear polished on both sides. Technical Data for Sapphire

  • Ideal substrate to grow cells for high pressure freezing
  • Suitable for CLEM – allows for fluorescent imaging of cells before high pressure freezing

Prod # Description Unit Price Order / Quote
39208 Sapphire Disc Substrate, Ø3mm x 0.05mm pkg/25 $310.45
Qty:
39209 Sapphire Disc Substrate, Ø6mm x 0.05mm pkg/25 517.95
Qty:




cryo freezer hats
3mm HPF Hat Storage Box
(#160-53)

cryo freezer hats
6mm HPF Hat Storage Box
(#160-61)

NEW Storage Boxes for HPF Hats

These metal cryo grid boxes are made of autoclave-compatible anodized aluminum, allowing for a more robust alternative to conventional plastic cryo grid boxes.

These storage boxes are the first for both 3mm and 6mm High Pressure Freezing Hats/Planchets/Carriers. The 3mm HPF Hat Storage Box (#160-53) has four pockets that can each hold up to two 3mm HPF hats. The 6mm HPF Hat Storage Box (#160-61) has two pockets that can each hold up to two 6mm HPF hats.

These boxes allow you to store your HPF samples securely under liquid nitrogen in a container with the same footprint as traditional Cryo-EM grid boxes, making it easily compatible with Cryo-EM workstations and storage systems (pucks, or similar).
Each storage box is:

  • Autoclave-compatible anodized aluminum
  • Available in six different colors
  • Permanently laser-marked with unique serial numbering to help in the tracking and organization of samples
These storage boxes come supplied in packs of six with either Low Profile Lids or without lids. The base can be used with other types of cryo grid box lids, such as Clear Pin Lids (#160-47C) or Metal Pin Lids (#160-47M).
 
Colors included
 cryo grid box handling rod
Available lid types

Prod # Description Unit Price Order / Quote
160-53 3mm HPF Hat Storage Boxes with Low Profile Lids pkg/6 $268.00
Qty:
160-53B 3mm HPF Hat Storage Boxes, Base Only pkg/6 209.00
Qty:
160-61 NEW 6mm HPF Hat Storage Boxes with Low Profile Lids pkg/6 284.00
Qty:
160-61B NEW 6mm HPF Hat Storage Boxes, Base Only pkg/6 231.00
Qty:

Prod # Description Unit Qty Price Order / Quote
160-45 Low Profile Lid each 1-99 $12.00
Qty:
100+ 11.00
160-45T Tool for Low Profile Lids each 1+ 15.00
Qty:
160-47C Clear Pin Lid each 1-99 12.00
Qty:
100+ 11.00
160-47M Metal Pin Lid each 1-99 17.00
Qty:
100+ 16.00




18037

NEW 1-Hexadecene

Prod # Description Unit Price Order / Quote
18037 1-Hexadecene, 25ml each $23.07
Qty:
SDS (544KB PDF)

CH3(CH2)13CH=CH2; [629-73-2]; MW 224.43
Lubricant for high pressure freezing hats. A light coat allows for easy hat separation and removal of sample.