Cryo-EM Supplies | Cryo-EM Tweezers
Cryo Safety Supplies

Cryo Embedding

Embedding Media	Uranyl Acetate	UV Cryo Chamber
PELCO® Freezer Hats NEW styles available!	NEW 1-Hexadecene	NEW PELCO® Sapphire Discs	Metal Universal HPF & Grid Boxes

LR Gold Resin

18183

$275.00

I - SDS (113KB PDF)

Resin (acrylic) only, no catalyst included. Requires Catalyst Prod. No. 18186: UV, benzoin methyl ether SDS (99KB PDF) (~365nm) (0.5% w/v); "blue light", benzyl (~460nm) (0.1% w/v); benzoyl peroxide SDS (113KB PDF) (1% w/v), cold-cure. Accurate measurements of catalyst are very important. LR Gold can be used at low temperatures (-25°C) and has proven very useful for immunogold procedures. Very low viscosity: 8cP. Applied to both LM (enzyme histochemistry) and EM (immunochemistry) techniques.
Technical Notes (1,816KB PDF)

Lowicryl^® HM-20 MonoStep^® (non polar)

For Sale in U.S.A. Only

18174

Lowicryl^® HM-20 MonoStep^® (non polar), 225g Pre-mixed, ready to use embedding media

each

$471.75

Qty:

I, O, T - SDS (121KB PDF) -

- Hazard Shipping Fees

Uranyl Acetate

Prod #

Description

Unit

Price

Order / Quote

19481

Uranyl Acetate, 25g

each

$224.00

Qty:

T, R (depleted) - SDS (162KB PDF)
PELCO® Technical Notes, Uranium Salts for Staining, Uranyl Acetate (pdf)

UO2(CH3COO)2 - 2H2O; [6159-44-0]; FW 424.14
Positive or negative stain, EM. May cause adverse mutagenic or teratogenic effects.

PELCO^® UVC3 Cryo Chamber

PELCO^® UVC3 Exterior/Control Panel

ultraviolet cryo chamber for low temperature curing / ultraviolet curing of polymers or resins

PELCO^® UVC3 Interior

Control Panel

UVC3 UV Cryo Chamber Screen Chart for Intuitive Programming

The PELCO^® UVC3 Cryo Chamber has been specifically designed to provide an economical yet precisely controlled low temperature environment for polymerization of polymers and resins for cryo specimen preparation. It can also be used for dehydration and infiltration of cryo specimens. This simple and functional unit has a large insulated chamber and comprises fully automatic temperature control for the polymerization process of embedding resins and polymers.

Features

Liquid crystal display and PLC control
Fan cooled electronic ballast for UV lamp tubes
Temperature range can be set from -10°C to -40°C (14°F to -40°F)
Includes memory function for Min/Max temperatures during any run
365nm ultraviolet lamps thermally shielded by dual pane Plexiglas
Holds up to 66 samples with BEEM^® Cryo Capsule Holders
Perfect for Unicryl, LR White, LR Gold, Lowicryls, JB-4, Quetols and all other resins cured by 365nm UV radiation
Universal voltage

Operation

Add dry ice to the chamber
Set temperature (-10°C to -40°C / 14°F to -40°F)
Turn on UV lamp and cure embedding resins

Specifications

UV Light Source	Two 15-watt self-filtering long wavelength tubes 1600 µW/cm_ of 365nm UV radiation at 15cm (6")
Temperature Control	-10°C to -40°C (14°F to -40°F) Typically +/- 1.5 °C
Control Type	LCD Display with PLC with function buttons
Exterior Dimensions	64.l L x 36.8 W x 48.9cm H (25.25" L x 14.50" W x 19.25" H)
Interior Dimensions	56.5 L x 30.5 W x 33.7cm H (22.25" L x 12" W x 13.4" H)
Power Requirements	110 - 240VAC, 50/60Hz (240V-0.5A, 110V-1A)
Weight	11.4 kg (25 lbs.) / Shipping weight: 18.2kg (40 lbs.)

Manufactured in USA

6202

PELCO^® UVC3 Cryo Chamber, 110 - 240VAC, 50/60Hz, includes 3 BEEM^® Cryo Capsule Holders

each

$2950.00

Qty:

6207

BEEM^® Cryo Capsule Holder for UV Curing

each

16.25

Qty:

10506

Flat Embedding Mold, PTFE, with metal frame, 16 cavities

each

76.50

Qty:

10506-10

Flat Embedding Mold, PTFE, with metal frame, 11 size 00 cavities

each

75.00

Qty:

10508

Flat Embedding Mold, PTFE, with metal frame, 3 cavities

each

76.50

Qty:

23065

Ultraviolet Tube, 15 watts (2 required)

each

51.25

Qty:

PELCO^® Freezer Hats for High Pressure Freezing

NEW Styles Available!


	Freezer Hats are used in High Pressure Freezers such as:
	Leica Wohlwend CryoCapCell ABRA RMC Boeckeler Balzers Bal-Tec			Conveniently store 3mm freezer hats in the Universal Metal HPF & Grid Storage Box (#160-53) shown at left.
	Planchet designs are roughly shown below in cross section. They are available in brass only. Depths of cavity in the planchets are 0.05, 0.1, 0.15, 0.2, 0.25 and 0.3mm. Available in both 3mm and 6mm diameter. Stacking two plachets, in various combinations, creates a cavity ranging from 0.05mm to 0.6mm. Please refer to the following technical note: Technical Notes, PELCO® Freezer Hats (739KB PDF)

Brass Planchet 0.1/0.2mm	Brass Planchet 0.0/0.3mm
Brass Planchet 0.15/0.15mm	Brass Planchet 0.05/0.25mm

39200

PELCO^® Freezer Hats, Brass, Specimen Holders, 0.1/0.2mm, Ø3mm

pkg/50

$299.00

Qty:

39201

PELCO^® Freezer Hats, Brass, Specimen Holders, 0.0/0.3mm, Ø3mm

pkg/50

299.00

Qty:

39204

NEW PELCO^® Freezer Hats, Brass, Specimen Holders, 0.05/0.25mm, Ø3mm

pkg/50

299.00

Qty:

39206

NEW PELCO^® Freezer Hats, Brass, Specimen Holders, 0.15/0.15mm, Ø3mm

pkg/50

299.00

Qty:

6mm Specimen Holders

39202

PELCO^® Freezer Hats, Brass, Specimen Holders, 0.1/0.2mm, Ø6mm

pkg/50

299.00

Qty:

39203

PELCO^® Freezer Hats, Brass, Specimen Holders, 0.0/0.3mm, Ø6mm

pkg/50

299.00

Qty:

39205

NEW PELCO^® Freezer Hats, Brass, Specimen Holders, 0.05/0.25mm, Ø6mm

pkg/50

299.00

Qty:

39207

NEW PELCO^® Freezer Hats, Brass, Specimen Holders, 0.15/0.15mm, Ø6mm

pkg/50

299.00

Qty:

NEW 1-Hexadecene

18037

$23.07

CH3(CH2)13CH=CH2; [629-73-2]; MW 224.43
Lubricant for high pressure freezing hats. A light coat allows for easy hat separation and removal of sample.

NEW PELCO^® Sapphire Discs

The PELCO^® Sapphire Discs are made from high purity clear sapphire (Al2O3), ground and optically clear polished on both sides. Technical Data for Sapphire

Ideal substrate to grow cells for high pressure freezing
Suitable for CLEM – allows for fluorescent imaging of cells before high pressure freezing

Prod #

Description

Unit

Price

Order / Quote

39208

Sapphire Disc Substrate, Ø3mm x 0.05mm

pkg/25

$310.45

Qty:

39209

Sapphire Disc Substrate, Ø6mm x 0.05mm

pkg/25

517.95

Qty:

Universal HPF & Grid Box
shown open with grids
and with Low Profile Lid
(#160-45)
cryo grid box handling rod

Universal HPF & Grid Box
can be used with
Metal & Clear Pin Lids
(#160-47M & #160-47C)

Metal Universal HPF & Grid Boxes

The Metal Universal HPF and Grid Boxes from NanoSoft are the first storage box for High Pressure Freezing Hats (3mm sized), and can also store both bare Cryo-EM grids and AutoGrids. The four pockets have two cross-shaped slots – one slot can hold either unclipped grids or AutoGrids, while the wider perpendicular slot holds HPF hats – making this box designed for all workflows, whether doing plunge freezing or HPF.

Each grid box is:

Autoclave-compatible anodized aluminum
Available in six different colors
Permanently laser-marked with unique serial numbering to help in the tracking and organization of samples

These Metal Universal HPF and Grid Boxes come supplied in packs of six with either low profile lids or without lids. The base can be used with other types of cryo grid box lids, such as Clear Pin Lids (#160-47C) or Metal Pin Lids (#160-47M).

The Low Profile Lid (#160-45) is made of anodized aluminum and is screwed on and off using Hex Tool (#160-45T). The low profile of the lid allows two grid boxes to be stacked in one pocket of a Cryo-EM grid storage puck, doubling your storage capacity. The anodized aluminum is less susceptible to static electricity than plastic lids, causing less sticking of grids to the underside of the lid. The Low Profile Lid is also compatible with all of our High Capacity Cryo Grid Boxes (#160-55, #160-56, #160-59).