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Cryo-TEM Supplies

Cryo Embedding



Embedding Media


Uranyl
Acetate
uv cryo chamber
UV Cryo Chamber
cryo freezer hats
PELCO® Freezer Hats
NEW styles available!
160-53
Metal Universal
HPF & Grid Boxes



LR Gold Resin

Prod # Description Unit Price Order / Quote
18183 LR Gold Resin, 500ml each $275.00
Qty:
I - SDS (113KB PDF)
Resin (acrylic) only, no catalyst included. Requires Catalyst Prod. No. 18186: UV, benzoin methyl ether SDS (99KB PDF) (~365nm) (0.5% w/v); "blue light", benzyl (~460nm) (0.1% w/v); benzoyl peroxide SDS (113KB PDF) (1% w/v), cold-cure. Accurate measurements of catalyst are very important. LR Gold can be used at low temperatures (-25°C) and has proven very useful for immunogold procedures. Very low viscosity: 8cP. Applied to both LM (enzyme histochemistry) and EM (immunochemistry) techniques.
Technical Notes (1,816KB PDF)




Lowicryl® HM-20 MonoStep® (non polar)

Prod # Description Unit Price Order / Quote
For Sale in U.S.A. Only
18174 Lowicryl® HM-20 MonoStep® (non polar), 225g Pre-mixed, ready to use embedding media each $471.75
Qty:
I, O, T - SDS (121KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees





Uranyl Acetate

Prod # Description Unit Price Order / Quote
19481 Uranyl Acetate, 25g each $224.00
Qty:
T, R (depleted) - SDS (162KB PDF)
PELCO® Technical Notes, Uranium Salts for Staining, Uranyl Acetate (pdf)
UO2(CH3COO)2 - 2H2O; [6159-44-0]; FW 424.14
Positive or negative stain, EM. May cause adverse mutagenic or teratogenic effects.




PELCO®UVC3 Cryo Chamber

PELCO UVC3 Cryo Chamber
PELCO® UVC3 Exterior/Control Panel
ultraviolet cryo chamber for low temperature curing / ultraviolet curing of polymers or resins
PELCO® UVC3 Interior


6202 UVC2 control panel
Control Panel


UVC3 UV Cryo Chamber Screen Chart for Intuitive Programming
The PELCO® UVC3 Cryo Chamber has been specifically designed to provide an economical yet precisely controlled low temperature environment for polymerization of polymers and resins for cryo specimen preparation. It can also be used for dehydration and infiltration of cryo specimens. This simple and functional unit has a large insulated chamber and comprises fully automatic temperature control for the polymerization process of embedding resins and polymers.

Features

  • Liquid crystal display and PLC control
  • Fan cooled electronic ballast for UV lamp tubes
  • Temperature range can be set from -10°C to -40°C (14°F to -40°F)
  • Includes memory function for Min/Max temperatures during any run
  • 365nm ultraviolet lamps thermally shielded by dual pane Plexiglas
  • Holds up to 66 samples with BEEM® Cryo Capsule Holders
  • Perfect for Unicryl, LR White, LR Gold, Lowicryls, JB-4, Quetols and all other resins cured by 365nm UV radiation
  • Universal voltage

Operation

  • Add dry ice to the chamber
  • Set temperature (-10°C to -40°C / 14°F to -40°F)
  • Turn on UV lamp and cure embedding resins

Specifications

UV Light Source Two 15-watt self-filtering long wavelength tubes
1600 µW/cm_ of 365nm UV radiation at 15cm (6")
Temperature Control -10°C to -40°C (14°F to -40°F) Typically +/- 1.5 °C
Control Type LCD Display with PLC with function buttons
Exterior Dimensions 64.l L x 36.8 W x 48.9cm H (25.25" L x 14.50" W x 19.25" H)
Interior Dimensions 56.5 L x 30.5 W x 33.7cm H (22.25" L x 12" W x 13.4" H)
Power Requirements 110 - 240VAC, 50/60Hz (240V-0.5A, 110V-1A)
Weight 11.4 kg (25 lbs.) / Shipping weight: 18.2kg (40 lbs.)

Manufactured in USA

Prod # Description Unit Price Order / Quote
6202 PELCO® UVC3 Cryo Chamber, 110 - 240VAC, 50/60Hz, includes 3 BEEM® Cryo Capsule Holders each $2950.00
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6207 BEEM® Cryo Capsule Holder for UV Curing each 16.25
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10506 Flat Embedding Mold, PTFE, with metal frame, 16 cavities each 76.50
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10506-10 Flat Embedding Mold, PTFE, with metal frame, 11 size 00 cavities each 75.00
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10508 Flat Embedding Mold, PTFE, with metal frame, 3 cavities each 76.50
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23065 Ultraviolet Tube, 15 watts (2 required) each 51.25
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PELCO® Freezer Hats for High Pressure Freezing

NEW styles available!


cryo freezer hats

Freezer Hats are used in High Pressure Freezers such as:

  • Leica
  • Balzers
  • Cressington
  • RMC

Planchet designs are roughly shown below in cross section. They are available in brass only. Depths of cavity in the planchets are 0.1, 0.2 and 0.3mm.

Using the two planchets, one on the other, in various combinations, permit the cavity thicknesses as illustrated below. Available in both 3mm and 6mm diameter.


planchet a

Brass Planchet "A"
planchet b

Brass Planchet "B"

freezer hat 1 freezer hat 2 freezer hat 3 freezer hat 4 freezer hat 5 freezer hat 6
0.1mm 0.2mm 0.3mm 0.4mm 0.5mm 0.6mm

Prod # Description Unit Price Order / Quote
3mm Specimen Holders
39200 PELCO® Freezer Hats, Brass, Specimen Holders, Type A, 3mm pkg/50 $272.50
Qty:
39201 PELCO® Freezer Hats, Brass, Specimen Holders, Type B, 3mm pkg/50 5.25
Qty:
6mm Specimen Holders
39202 NEW PELCO® Freezer Hats, Brass, Specimen Holders, Type A, 6mm pkg/50 277.00
Qty:
39203 NEW PELCO® Freezer Hats, Brass, Specimen Holders, Type B, 6mm pkg/50 277.00
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cryo grid box handling rod
Universal HPF & Grid Box
shown open w/grids and with
Low Profile Lid
(#160-45)

cryo grid box handling rod
Universal HPF & Grid Box
can be used with
Metal & Clear Pin Lids
(#160-47M & #160-47C)


Metal Universal HPF & Grid Boxes

The Metal Universal HPF and Grid Boxes from NanoSoft are the first storage box for High Pressure Freezing Hats (3mm sized), and can also store both bare Cryo-EM grids and AutoGrids. The four pockets have two cross-shaped slots – one slot can hold either unclipped grids or AutoGrids, while the wider perpendicular slot holds HPF hats – making this box designed for all workflows, whether doing plunge freezing or HPF.

Each grid box is:
  • Autoclave-compatible anodized aluminum
  • Available in six different colors
  • Permanently laser-marked with unique serial numbering to help in the tracking and organization of samples

These Metal Universal HPF and Grid Boxes come supplied in packs of six with either Low Profile Lids or without lids. The base can be used with other types of cryo grid box lids, such as Clear Pin Lids (#160-47C) or Metal Pin Lids (#160-47M).

The Low Profile Lid (#160-45) is made of anodized aluminum and is screwed on and off using Hex Tool (#160-45T). The low profile of the lid allows two grid boxes to be stacked in one pocket of a Cryo-EM grid storage puck, doubling your storage capacity. The anodized aluminum is less susceptible to static electricity than plastic lids, causing less sticking of grids to the underside of the lid. The Low Profile Lid is also compatible with all of our High Capacity Cryo Grid Boxes (#160-55, #160-56, #160-59).


(#160-53)

(#160-53B)

Prod # Description Unit Price Order / Quote
160-53 Metal Universal HPF & Grid Boxes with Low Profile Lids pkg/6 $268.00
Qty:
160-53B Metal Universal HPF & Grid Boxes, Base Only pkg/6 209.00
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Prod # Description Unit Qty Price Order / Quote
160-45 Low Profile Lid each 1-99 $12.00
Qty:
100+ 11.00
160-45T Tool for Low Profile Lids each 1+ 15.00
Qty: