PELCO^® Gold Nanoparticles - Gold Colloids

Our new line of PELCO^® Gold Nanoparticles are precisely engineered to give monodispersed, uniform gold spheres with a narrow size distribution.

• Gold nanoparticles ranging in size from 5nm - 250nm (Coefficient of Variance < 12%)
• Available in 20mL and 100mL volumes at ~0.05 mg/mL and an optical density (OD) = 1
• Non-functionalized and citrate capped with exceptional protein binding characteristics
• Polydispersity Index (PDI) < 0.20 with an absorbance (λmax) of 510-570nm depending on particle size
• Supplied in 0.1mM Phosphate-Buffered Saline (0.01X PBS)
• Standard nanoparticles >95.0% reactant free while our high purity Endotoxin Free nanoparticles are >99.0% reactant and endotoxin free

Suitable for use in applications such as peptide/protein gold conjugate development, immunoblotting, Surface Enhanced Raman Spectroscopy, light microscopy, and electron microscopy. Endotoxin free nanoparticles are optimized for sensitive applications such as cellular and toxicity studies, immunological studies, aseptic assays, and have high protein binding efficiency.

15nm	30nm	60nm	100nm

Transmission electron microscopy (TEM) images of gold nanoparticles


UV-Vis spectrum of gold nanoparticles

Diameter (nm) Peak SPR Wavelength (nm) Particles/ml Wt. Conc. (mg/ml) Molar Ext (M-1cm-1) Size Variation (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/ Volume Ratio Particle Mass (g) Molar Mass (g/mole) Molar Conc. 5 515-520 5.47 x 1013 6.94 x 10-2 1.10 x 107 <15% 6.54 x 101 7.85 x 101 1.2 1.27 x 10-18 7.64 x 105 9.08 x 10-8 10 515-520 5.98 x 1012 6.07 x 10-2 1.01 x 108 <15% 5.24 x 102 3.14 x 102 0.6 1.02 x 10-17 6.11 x 106 9.93 x 10-9 15 520 1.64 x 1012 5.61 x 10-2 3.67 x 108 <12% 1.77 x 103 7.07 x 102 0.4 3.43 x 10-17 2.06 x 107 2.72 x 10-9 20 524 6.54 x 1011 5.31 x 10-2 9.21 x 108 <12% 4.19 x 103 1.26 x 103 0.3 8.12 x 10-17 4.89 x 107 1.09 x 10-9 30 526 1.79 x 1011 4.91 x 10-2 3.36 x 109 <12% 1.41 x 104 2.83 x 103 0.2 2.74 x 10-16 1.65 x 108 2.98 x 10-10 40 530 7.15 x 1010 4.65 x 10-2 8.42 x 109 <12% 3.35 x 104 5.03 x 103 0.15 6.50 x 10-16 3.91 x 108 1.19 x 10-10 50 535 3.51 x 1010 4.45 x 10-2 1.72 x 1010 <10% 6.54 x 104 7.85 x 103 0.12 1.27 x 10-15 7.64 x 108 5.83 x 10-11 60 540 1.96 x 1010 4.30 x 10-2 3.07 x 1010 <10% 1.13 x 105 1.13 x 104 0.1 2.19 x 10-15 1.32 x 109 3.25 x 10-11 80 553 7.82 x 109 4.06 x 10-2 7.70 x 1010 <10% 2.68 x 105 2.01 x 104 0.075 5.20 x 10-15 3.13 x 109 1.30 x 10-11 100 572 3.84 x 109 3.89 x 10-2 1.57 x 1011 <8% 5.24 x 105 3.14 x 104 0.06 1.02 x 10-14 6.11 x 109 6.37 x 10-12 150   3.60 x 109 1.20 x 10-1   <8% 1.77 x 106 7.07 x 104 0.04 3.43 x 10-14 2.06 x 1010 5.98 x 10-12 200   1.91 x 109 1.55 x 10-1   <8% 4.19 x 106 1.26 x 105 0.03 8.12 x 10-14 4.89 x 1010 3.17 x 10-12 250   7.08 x 108 1.13 x 10-1   <8% 8.18 x 106 1.96 x 105 0.024 1.59 x 10-13 9.55 x 1010 1.18 x 10-12

NO FRIDAY/WEEKEND SHIPMENTS All shipments of Gold Conjugate products must be for next day delivery due to temperature requirements.

PELCO® Gold Nanoparticles

Standard Gold Nanoparticles

Endotoxin Free Gold Nanoparticles

Passive Protein Conjugation Kits

Gold Protein Detection Kit

Silver Enhancement Kits

PELCO^® Gold Nanoparticle Conjugation Kits - Passive Adsorption

Stable gold nanoparticle conjugates can readily be prepared by passive adsorption of a protein to the gold nanoparticle surface, which is mediated by electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal gold.

Gold nanoparticle conjugates have been used for a wide range of biological applications including the use as probes in light and electron microscopy and for immunoblotting.

This kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale-up production of 20ml of conjugate at a final optical density of OD3.

Each kit includes:

• Standard Gold Nanoparticles of selected size (100ml, OD1)
• 12 Conjugation Optimization Buffers (1.5ml each)
• 10% NaCl (100ml)
• Protein Resuspension Buffer
• Detailed conjugation optimization protocol

Note: Only use with PELCO^® Standard Gold Nanoparticles

PELCO^® Gold Protein Detection Kit

The PELCO^® Gold Protein Detection Kit is a stabilized gold solution specially developed to stain proteins blotted onto nitrocellulose or polyvinylidene difluoride (PVDF) membranes.

The negatively charged gold particles bind to protein(s) on the membrane through ionic and hydrophobic interaction with much higher affinity than to the membrane itself. Accumulation of gold particles onto the bound proteins produces dark permanent red bands, with better sensitivity (down to 1ng, protein dependent).

The kit is provided ready to use, no reconstitution or dilution required, with enough to stain ~25 blots. When used in conjunction with PELCO® Silver Enhancement Kit for Membranes (#17006) below, a further improvement in sensitivity (10 to 100 times) can be achieved.

Each kit includes:

• 500ml Ready-to-Use Gold Membrane Stain
• 100ml Wash Buffer (100X)

PELCO^® Silver Enhancement Kits

Kits for silver enhancement of gold and silver conjugate labeled samples in dot blot, Western Blots, and microscopy. Using these straightforward kits, sensitivity in the picogram range can be achieved in 5–8-minute staining time all while maintaining a low background.

Amplification of the gold and silver nanoparticle label is a result of the deposition of silver on the nanoparticle surface during the reaction and increases the sensitivity of the nanoparticle conjugate labeling up to 100-fold. This allows for detection of scarce targets.

Two silver enhancement kits are available, one for microscopy and one for membranes. The Microscopy Kit is ideal for use in light microscopy and electron microscopy applications, and includes 50ml of working solution (staining of 50-100 microscope slides). The Membrane Kit is ideal for applications such as Western Blots and dot blot assays, and includes 500ml of working solution (staining of ~100 membrane strips or 10-20 membranes).