PELCO^® Silver Nanoparticles - Silver Colloids

Our new line of PELCO^® Silver Nanoparticles are precisely engineered to give monodispersed, uniform silver spheres with a narrow size distribution.

• Silver nanoparticles ranging in size from 10nm - 100nm (Coefficient of Variance < 15%)
• Available in 20mL and 100mL volumes at ~0.02 mg/mL
• Non-functionalized and citrate capped with exceptional protein binding characteristics
• Polydispersity Index (PDI) < 0.20 with an absorbance (λmax) of 390-490nm depending on particle size
• Supplied in 2mM sodium citrate
• Standard nanoparticles >95.0% reactant free while our high purity Endotoxin Free nanoparticles are >99.0% reactant and endotoxin free

Silver nanoparticles are a great alternative to gold nanoparticles if absorption maximum is desired in between 400-500nm. Absorption in this lower range also allows for an increased dynamic range when developing multiplex experiments. Silver nanoparticles retain the same protein and other ligand binding properties of gold nanoparticles. Suitable for use in applications such as photovoltaics, biological sensor development, peptide/protein silver conjugate development, immunoblotting, Surface Enhanced Raman Spectroscopy, light microscopy, and electron microscopy. Endotoxin free nanoparticles are optimized for sensitive applications such as cellular and toxicity studies, immunological studies, and aseptic assays.

Store product in the dark at 4°C. DO NOT FREEZE. Freezing will cause irreversible aggregation of the silver nanoparticles. Stable for at least 6 months if unopened and stored as specified.

10nm

30nm

60nm

100nm

UV-Vis spectrum of silver nanoparticles

Diameter (nm) Peak SPR Wavelength (nm) Particles/ml Wt. Conc. (mg/ml) Size Variation (+/-nm) Particle Volume (nm3) Surface Area (nm2) Surface/ Volume Ratio Particle Mass (g) Molar Mass (g/mole) Molar Conc. 10 390-405 3.6 x 1012 2.0 x 10-2 <18% 5.24 x 102 3.14 x 102 0.60 5.49 x 10-18 3.31 x 106 5.98 x 10-9 20 390-410 4.6 x 1011 2.0 x 10-2 <15% 4.19 x 103 1.26 x 103 0.30 4.39 x 10-17 2.65 x 107 7.64 x 10-10 30 400-410 1.4 x 1011 2.0 x 10-2 <15% 1.41 x 104 2.83 x 103 0.20 1.48 x 10-16 8.93 x 107 2.32 x 10-10 40 405-425 5.7 x 1010 2.0 x 10-2 <15% 3.35 x 104 5.03 x 103 0.15 3.52 x 10-16 2.12 x 108 9.47 x 10-11 50 410-430 2.9 x 1010 2.0 x 10-2 <12% 6.54 x 104 7.85 x 103 0.12 6.87 x 10-16 4.13 x 108 4.28 x 10-11 60 425-450 1.7 x 1010 2.0 x 10-2 <12% 1.13 x 105 1.13 x 104 0.10 1.19 x 10-15 7.14 x 108 2.82 x 10-11 80 440-480 7.1 x 109 2.0 x 10-2 <12% 2.68 x 105 2.01 x 104 0.075 2.81 x 10-15 1.69 x 109 1.18 x 10-11 100 480-520 3.6 x 109 2.0 x 10-2 <10% 5.24 x 105 3.14 x 104 0.06 5.49 x 10-15 3.31 x 109 5.98 x 10-12

NO FRIDAY/WEEKEND SHIPMENTS All shipments of Silver Nanoparticle products must be for next day delivery due to temperature requirements.

PELCO® Silver Nanoparticles

Standard Silver Nanoparticles

Endotoxin Free Silver Nanoparticles

Passive Protein Conjugation Kits

Silver Enhancement Kits

PELCO^® Silver Nanoparticle Conjugation Kits - Passive Adsorption

Stable silver nanoparticle conjugates can readily be prepared by passive adsorption of a protein to the silver nanoparticle surface, which is mediated by electrostatic and hydrophobic interactions between the protein and the surface layer of the colloidal silver.

This kit contains enough material to optimize 10 protein conjugates, or one optimization reaction and scale-up production of 20ml of conjugate at a final concentration of 0.06 mg/ml silver nanoparticles.

Each kit includes:

• Standard Silver Nanoparticles of selected size (100ml, 0.02 mg/ml)
• 12 Conjugation Optimization Buffers (1.5ml each)
• 10% NaCl (100ml)
• Protein Resuspension Buffer
• Detailed conjugation optimization protocol

Note: Only use with PELCO^® Standard Silver Nanoparticles

PELCO^® Silver Enhancement Kits

Kits for silver enhancement of gold and silver conjugate labeled samples in dot blot, Western Blots, and microscopy. Using these straightforward kits, sensitivity in the picogram range can be achieved in 5–8-minute staining time all while maintaining a low background.

Amplification of the gold and silver nanoparticle label is a result of the deposition of silver on the nanoparticle surface during the reaction and increases the sensitivity of the nanoparticle conjugate labeling up to 100-fold. This allows for detection of scarce targets.

Two silver enhancement kits are available, one for microscopy and one for membranes. The Microscopy Kit is ideal for use in light microscopy and electron microscopy applications, and includes 50ml of working solution (staining of 50-100 microscope slides). The Membrane Kit is ideal for applications such as Western Blots and dot blot assays, and includes 500ml of working solution (staining of ~100 membrane strips or 10-20 membranes).