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Calibration Overview
SEM Calibration Overview

SEM High Resolution, Gold on Carbon
Test Specimens

gold on carbon test specimen
Resolution Test Specimen,
Gold on Carbon 1 		scanning electron microscopy test specimen
High Resolution Test Specimen,
Gold on Carbon 2 		sem ultrahigh gold on carbon
Ultra High Resolution Test Specimen,
Gold on Carbon 3 		low voltage sem resolution specimen
Low Voltage Resolution Test Specimen,
Gold on Carbon 4 		gold on carbon standard
Medium Resolution Test Specimen,
Gold on Carbon 5

Scanning electron microscope (SEM and Focused Ion Beam (FIB) system resolution is defined in terms of a combination of criteria: resolution and number of gray levels in the image. This is to ensure that the resolution has not been distorted by using too much contrast to maximize visibility of the edges. High resolution images ideally show fine details together with a low noise and a good range of contrast levels. The widely used gold on carbon test specimens are excellent for SEM, FESEM and FIB resolution testing. The gold particles, with superior feature details, generate high secondary and/or backscatter electron signals with excellent contrast against the carbon background. Five different special resolution testing specimens are available with different gold particle sizes for different resolution levels. Available unmounted or conveniently mounted on most common SEMs stubs.

Store your calibration and test specimens in a vacuum desiccator such as the #16179 PELCO® SEM Sample Stub Vacuum Desiccator.

PELCO® Technical Notes for Gold on Carbon SEM Resolution Test Specimens (PDF)




gold on carbon test specimen
see mount selections, types A-R

Resolution Test Specimen, Gold on Carbon 1

particle size range from approximately 5nm to 150nm

Each specimen has a square grid pattern with large crystals in the center of each square and very fine crystals at the edges of each grid (as illustrated). Thus, medium and high resolution gap tests are performed on the same specimen. The larger crystals show facets which allow assessment of the gray level reproduction available at high resolution. 6mm dia. x 2mm.

Prod # Description Unit Price Order / Quote
617 Resolution Gold on Carbon, Specimen 1, Unmounted each $162.50
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617-A Resolution Gold on Carbon, Specimen 1, Mount A each 172.50
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617-B Resolution Gold on Carbon, Specimen 1, Mount B each 172.50
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617-C Resolution Gold on Carbon, Specimen 1, Mount C each 172.50
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617-D Resolution Gold on Carbon, Specimen 1, Mount D each 172.50
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617-E Resolution Gold on Carbon, Specimen 1, Mount E each 172.50
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617-F Resolution Gold on Carbon, Specimen 1, Mount F each 172.50
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617-G Resolution Gold on Carbon, Specimen 1, You Supply Mount each 262.50
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617-K Resolution Gold on Carbon, Specimen 1, Mount K each 172.50
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617-L Resolution Gold on Carbon, Specimen 1, Mount L each 172.50
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617-M Resolution Gold on Carbon, Specimen 1, Mount M each 172.50
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617-O Resolution Gold on Carbon, Specimen 1, Mount O each 172.50
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617-P Resolution Gold on Carbon, Specimen 1, Mount P each 172.50
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617-Q Resolution Gold on Carbon, Specimen 1, Mount Q each 172.50
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617-R Resolution Gold on Carbon, Specimen 1, Mount R each 172.50
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scanning electron microscopy test specimen
see mount selections, types A-R

High Resolution Test Specimen, Gold on Carbon 2

particle size range from <3nm to 50nm

Particularly suited for assessing the image quality of high resolution SEMs, such as those fitted with a field emission source. A magnification of at least x80,000 is required to clearly resolve the gold particles. 6mm dia. x 2mm.

Prod # Description Unit Price Order / Quote
617-2 High Resolution Gold on Carbon Spec. 2, Unmounted each $385.00
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617-2A High Resolution Gold on Carbon Spec. 2, Mount A each 395.00
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617-2B High Resolution Gold on Carbon Spec. 2, Mount B each 395.00
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617-2C High Resolution Gold on Carbon Spec. 2, Mount C each 395.00
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617-2D High Resolution Gold on Carbon Spec. 2, Mount D each 395.00
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617-2E High Resolution Gold on Carbon Spec. 2, Mount E each 395.00
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617-2F High Resolution Gold on Carbon Spec. 2, Mount F each 395.00
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617-2G High Resolution Gold on Carbon Spec. 2, You Supply Mount each 495.00
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617-2K High Resolution Gold on Carbon Spec. 2, Mount K each 395.00
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617-2L High Resolution Gold on Carbon Spec. 2, Mount L each 395.00
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617-2M High Resolution Gold on Carbon Spec. 2, Mount M each 395.00
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617-2O High Resolution Gold on Carbon Spec. 2, Mount O each 395.00
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617-2P High Resolution Gold on Carbon Spec. 2, Mount P each 395.00
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617-2Q High Resolution Gold on Carbon Spec. 2, Mount Q each 395.00
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617-2R High Resolution Gold on Carbon Spec. 2, Mount R each 395.00
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sem ultrahigh gold on carbon
see mount selections, types A-R

Ultra High Resolution Test Specimen, Gold on Carbon 3

particle size range from <2nm to 30nm

For ultra high resolution performance testing, this specimen has a smaller gold island particle size compared to the 617 specimen described previously. Suitable for testing field emission SEM's at instrument magnifications of x100,000 and above. 6mm dia. x 2mm.

Prod # Description Unit Price Order / Quote
617-3 Ultra High Resolution Gold on Carbon Spec. 3, Unmounted each $380.00
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617-3A Ultra High Resolution Gold on Carbon Spec. 3, Mount A each 390.00
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617-3B Ultra High Resolution Gold on Carbon Spec. 3, Mount B each 390.00
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617-3C Ultra High Resolution Gold on Carbon Spec. 3, Mount C each 390.00
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617-3D Ultra High Resolution Gold on Carbon Spec. 3, Mount D each 390.00
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617-3E Ultra High Resolution Gold on Carbon Spec. 3, Mount E each 390.00
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617-3F Ultra High Resolution Gold on Carbon Spec. 3, Mount F each 390.00
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617-3G Ultra High Resolution Gold on Carbon Spec. 3, You Supply Mount each 483.00
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617-3K Ultra High Resolution Gold on Carbon Spec. 3, Mount K each 390.00
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617-3L Ultra High Resolution Gold on Carbon Spec. 3, Mount L each 390.00
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617-3M Ultra High Resolution Gold on Carbon Spec. 3, Mount M each 390.00
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617-3O Ultra High Resolution Gold on Carbon Spec. 3, Mount O each 390.00
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617-3P Ultra High Resolution Gold on Carbon Spec. 3, Mount P each 390.00
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617-3Q Ultra High Resolution Gold on Carbon Spec. 3, Mount Q each 390.00
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617-3R Ultra High Resolution Gold on Carbon Spec. 3, Mount R each 390.00
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low voltage sem resolution specimen
see mount selections, types A-R

Low Voltage Resolution Test Specimen, Gold on Carbon 4

particle sizes range from 30nm to 500nm

When operating at low accelerating voltages or using older instruments, difficulties may be experienced in imaging the standard gold on carbon resolution specimens. This may be due to inferior resolution at low voltage or poor signal to noise ratio when operating at high scanning rates with small spot sizes. The larger gold islands give high contrast while retaining small gaps for resolution measurement. This makes the specimen easier to use at non-optimal operating conditions. 6mm dia. x 2mm.

Prod # Description Unit Price Order / Quote
617-4 Low Voltage Resolution Gold on Carbon Spec. 4, Unmounted each $178.00
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617-4A Low Voltage Resolution Gold on Carbon Spec. 4, Mount A each 188.00
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617-4B Low Voltage Resolution Gold on Carbon Spec. 4, Mount B each 188.00
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617-4C Low Voltage Resolution Gold on Carbon Spec. 4, Mount C each 188.00
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617-4D Low Voltage Resolution Gold on Carbon Spec. 4, Mount D each 188.00
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617-4E Low Voltage Resolution Gold on Carbon Spec. 4, Mount E each 188.00
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617-4F Low Voltage Resolution Gold on Carbon Spec. 4, Mount F each 188.00
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617-4G Low Voltage Resolution Gold on Carbon Spec. 4, You Supply Mount each 278.00
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617-4K Low Voltage Resolution Gold on Carbon Spec. 4, Mount K each 188.00
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617-4L Low Voltage Resolution Gold on Carbon Spec. 4, Mount L each 188.00
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617-4M Low Voltage Resolution Gold on Carbon Spec. 4, Mount M each 188.00
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617-4O Low Voltage Resolution Gold on Carbon Spec. 4, Mount O each 188.00
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617-4P Low Voltage Resolution Gold on Carbon Spec. 4, Mount P each 188.00
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617-4Q Low Voltage Resolution Gold on Carbon Spec. 4, Mount Q each 188.00
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617-4R Low Voltage Resolution Gold on Carbon Spec. 4, Mount R each 188.00
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gold on carbon standard
see mount selections, types A-R

Medium Resolution Test Specimen, Gold on Carbon 5

The larger gold particles, with clear separation, provide an excellent structure to setup the microscope. Particle size range 0.1-1µm, for scanning electron microscopes and high magnification light microscopes. Magnification range 800-10,000x. 6.4mm dia. x 0.7mm.

Prod # Description Unit Price Order / Quote
623 Medium Resolution Gold on Carbon Specimen 5, Unmounted each $212.00
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623-A Medium Resolution Gold on Carbon Specimen 5, Mount A each 215.00
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623-B Medium Resolution Gold on Carbon Specimen 5, Mount B each 215.00
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623-C Medium Resolution Gold on Carbon Specimen 5, Mount C each 215.00
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623-D Medium Resolution Gold on Carbon Specimen 5, Mount D each 215.00
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623-E Medium Resolution Gold on Carbon Specimen 5, Mount E each 215.00
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623-F Medium Resolution Gold on Carbon Specimen 5, Mount F each 215.00
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623-G Medium Resolution Gold on Carbon Specimen 5, Mount G, You Supply Mount each 318.00
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623-K Medium Resolution Gold on Carbon Specimen 5, Mount K each 215.00
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623-L Medium Resolution Gold on Carbon Specimen 5, Mount L each 215.00
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623-M Medium Resolution Gold on Carbon Specimen 5, Mount M each 215.00
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623-O Medium Resolution Gold on Carbon Specimen 5, Mount O each 215.00
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623-P Medium Resolution Gold on Carbon Specimen 5, Mount P each 215.00
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623-Q Medium Resolution Gold on Carbon Specimen 5, Mount Q each 215.00
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623-R Medium Resolution Gold on Carbon Specimen 5, Mount R each 215.00
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