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Calibration Overview
AFM Calibration Overview Page
SEM Calibration Overview Page

2D Holographic Array Standards

Very High Resolution 144nm 2D Calibration Standard Very High Resolution 300nm` 2D Calibration Standard
Model 2D/144nm Pitch Model 2D/300nm Pitch

Precision, holographic pattern providing accurate calibration in the horizontal plane for very high resolution, nanometer-scale measurements with 144nm and 300nm pitch.

144nm Very High Resolution 2D Calibration Standard
for AFM, STM, Auger, FIB, and SEM

Period: 144nm pitch, two-dimensional array. Accurate to ±1nm. Refer to calibration certificate for actual pitch.
Surface: Aluminum bumps on Silicon, 4x3mm die. Bump height (about 90nm) and width (about 75nm) are not calibrated.
Usability: the calibrated pattern covers the entire chip. There is sufficient usable area to make tens of thousands of measurements without reusing any areas altered or contaminated by previous scans.
AFM: use in contact, intermittent contact (TappingMode™ ) and other modes with image sizes from 250nm to 10µm. Available unmounted or mounted on 12mm steel disks.
SEM: this specimen works well at all accelerating voltages. Normally supplied unmounted. Can be mounted on a stub of your choice of SEM Mount Selection A-R.
Model 2D: This Calibration Reference specimen comes with a non-traceable, manufacturer’s certificate. This states the average period, based on batch measurements. See Sample Certificate
Model 2DUTC: This Traceable, Certified Standard is a select grade. Each standard is individually measured in comparison with a similar specimen calibrated at PTB. (PTB, Physikalisch-Technischen Bundesanstalt, is the German counterpart of NIST.) The uncertainty of single pitch values is typically ±1.4nm (95% confidence interval). Multi-pitch measurements provide the usual square-root of N improvement in precision.
Model 2DUTC Sample Certificate (PDF 111KB)

Easy to use

The 2D holographic Array with 144nm is recommended because of the unique characteristics that make it especially easy to use. The pattern is durable and allows for scanning in contact mode, which means that calibration and measurements are faster. This is the only high resolution 2D calibration standard we know of that has all of the following characteristics that are needed for ease of use:

  • 2-dimensional array for simultaneous calibration of X and Y axes
  • pitch << 500nm
  • array of bumps means the image contrast is high even when the probe tip is slightly dull
  • high contrast in contact mode scans
  • pattern covers the entire die, no need to hunt for the scan area.

AFM images:

Tapping Mode 3µm AFM scan Contact Mode 5µm AFM scan

During scanning in contact mode using a 0.5 N/m SiN cantilever, we did not notice any surface or tip wear affecting the image.

Further information

SEM Images

High Magnification

The following image (inset) was captured with a magnification setting of 100 kX and accelerating voltage 10 kV. Outside Image 20 kV.

Medium Magnification

At 5 kX, the individual bumps were still well-resolved. Large fields of view show how few defects are present. The most common defects are single missing bumps or a single extra bump inserted between lattice positions. Two vacancies are present in the image shown here.

Available unmounted, on a 12mm AFM disc or on SEM stubs: SEM Mount Selection A-R.

Prod # Description Unit Price Order / Quote
SEM Reference Standards, Certified, Non-traceable, Unmounted or Mounted:
16465-2D 144nm 2D Pattern Calibration Standard, unmounted each $1442.00
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16465-2D-A 144nm 2D Pattern Calibration Standard on Mount A each 1593.00
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16465-2D-B 144nm 2D Pattern Calibration Standard on Mount B each P.O.R.
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16465-2D-C 144nm 2D Pattern Calibration Standard on Mount C each P.O.R.
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16465-2D-D 144nm 2D Pattern Calibration Standard on Mount D each 1593.00
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16465-2D-E 144nm 2D Pattern Calibration Standard on Mount E each 1593.00
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16465-2D-F 144nm 2D Pattern Calibration Standard on Mount F each 1431.00
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16465-2D-G 144nm 2D Pattern Calibration Standard on Mount G, you supply mount each 1593.00
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16465-2D-K 144nm 2D Pattern Calibration Standard on Mount K each 1593.00
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16465-2D-L 144nm 2D Pattern Calibration Standard on Mount L each 1593.00
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16465-2D-M 144nm 2D Pattern Calibration Standard on Mount M each 2189.00
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16465-2D-O 144nm 2D Pattern Calibration Standard on Mount O each 2189.00
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16465-2D-P 144nm 2D Pattern Calibration Standard on Mount P each 2189.00
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16465-2D-Q 144nm 2D Pattern Calibration Standard on Mount Q each 2189.00
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16465-2D-R 144nm 2D Pattern Calibration Standard on Mount R each 1431.00
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AFM Reference Standard, Certified, Non-traceable, Mounted on Disk:
16465-2D-AFM 144nm 2D Pattern Calibration Standard on 12mm steel disc each 1593.00
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SEM Reference Standards, Certified, Traceable, Calibration Certificate Provided, Unmounted or Mounted:
16465-2DUTC 144nm 2DUTC Pattern Calibration Standard, unmounted, with certificate each P.O.R.
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16465-2DUTC-A 144nm 2DUTC Pattern Calibration Standard, on Mount A, with certificate each P.O.R.
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16465-2DUTC-B 144nm 2DUTC Pattern Calibration Standard, on Mount B, with certificate each P.O.R.
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16465-2DUTC-C 144nm 2DUTC Pattern Calibration Standard, on Mount C, with certificate each P.O.R.
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16465-2DUTC-D 144nm 2DUTC Pattern Calibration Standard, on Mount D, with certificate each P.O.R.
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16465-2DUTC-E 144nm 2DUTC Pattern Calibration Standard, on Mount E, with certificate each P.O.R.
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16465-2DUTC-F 144nm 2DUTC Pattern Calibration Standard, on Mount F, with certificate each P.O.R.
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16465-2DUTC-G 144nm 2DUTC Pattern Calibration Standard, on Mount G, you supply mount, with certificate each P.O.R.
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16465-2DUTC-K 144nm 2DUTC Pattern Calibration Standard, on Mount K, with certificate each P.O.R.
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16465-2DUTC-L 144nm 2DUTC Pattern Calibration Standard, on Mount L, with certificate each P.O.R.
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16465-2DUTC-M 144nm 2DUTC Pattern Calibration Standard, on Mount M, with certificate each P.O.R.
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16465-2DUTC-O 144nm 2DUTC Pattern Calibration Standard, on Mount O, with certificate each P.O.R.
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16465-2DUTC-P 144nm 2DUTC Pattern Calibration Standard, on Mount P, with certificate each P.O.R.
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16465-2DUTC-Q 144nm 2DUTC Pattern Calibration Standard, on Mount Q, with certificate each P.O.R.
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16465-2DUTC-R 144nm 2DUTC Pattern Calibration Standard, on Mount R, with certificate each P.O.R.
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AFM Reference Standard, Certified, Traceable, Calibration Certificate Provided, Mounted on Disk:
16465-2DUTC-AFM 144nm 2DUTC Pattern Calibration Standard, on 12mm steel disk, with certificate each P.O.R.
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300nm Pitch High Resolution 2D Calibration Standard
for AFM, STEM, SEM, Auger and FIB.

Very High Resolution 2D Calibration Standard

Period: 300nm pitch nominal, two dimensional array accurate to ±1nm. Calibration certificate will give the actual pitch of the standard.
Surface Structure: Aluminum bumps on Silicon, 4x3mm die: Bump height (about 50nm) and width (about 150nm) not calibrated.
Usability: The calibrated pattern covers the entire chip. There is sufficient usable area to make thousands of measurements without reusing any areas contaminated or altered by previous scans.
AFM: Use in contact, tapping and other modes with image sizes from 500nm to 20nm. Mounted on a 12mm steel AFM disk.
SEM: Auger, FIB: Can be used for a wide range of accelerating voltage (1kV-20kV) and calibrates images from 5kX to 200kX. Can be supplied unmounted or mounted on an SEM stub of your choice, SEM Mount Selection A-R.
Certification: Supplied with a non-traceable manufacturer's certificate stating average pitch, based on batch measurements. See Sample Certificate

Prod # Description Unit Price Order / Quote
300nm 2D AFM Reference Standard, Certified, Non-traceable, Mounted on Disk:
16475-1AFM 300nm 2D Resolution AFM Reference Standard on 12mm steel disc each $1359.95
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300nm 2D SEM, Auger and FIB Reference Standard, Certified, Non-traceable, Unmounted or Mounted
16475-1 300nm 2D Resolution AFM Reference Standard, unmounted each 1349.50
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16475-1A 300nm 2D Resolution AFM Reference Standard on Mount A each 1514.00
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16475-1B 300nm 2D Resolution AFM Reference Standard on Mount B each 1492.00
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16475-1C 300nm 2D Resolution AFM Reference Standard on Mount C each 1492.00
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16475-1D 300nm 2D Resolution AFM Reference Standard on Mount D each 1492.00
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16475-1E 300nm 2D Resolution AFM Reference Standard on Mount E each P.O.R.
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16475-1F 300nm 2D Resolution AFM Reference Standard on Mount F each 1492.00
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16475-1G 300nm 2D Resolution AFM Reference Standard on Mount G, you supply mount each 1613.00
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16475-1K 300nm 2D Resolution AFM Reference Standard on Mount K each 1359.95
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16475-1L 300nm 2D Resolution AFM Reference Standard on Mount L each 1492.00
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16475-1M 300nm 2D Resolution AFM Reference Standard on Mount M each 1492.00
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16475-1O 300nm 2D Resolution AFM Reference Standard on Mount O each 1492.00
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16475-1P 300nm 2D Resolution AFM Reference Standard on Mount P each 1492.00
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16475-1Q 300nm 2D Resolution AFM Reference Standard on Mount Q each 1492.00
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16475-1R 300nm 2D Resolution AFM Reference Standard on Mount R each 1233.00
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