PELCO Glass Bottom Dishes by WillCo Wells

Glass Bottom Dish Assembly Kits
Build your own dishes
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Technical Notes
for PELCO® Glass Bottom Dishes
Positioning of Life Cells, including Ova, Sperm Cells & Embryos

Glass Bottom Dishes are used in the fields of Life Science and Environmental Research, Molecular and Cell Biology, Cell Culture and Cell Physiology, Biotechnology, Neurology, Pathology, Pharmacology and more.

These sterile tissue culture grade polystyrene glass bottom WillCo Wells Petri dishes with lid are available in two sizes, 35 x 10mm and 50 x 7mm, with clear or black plastic sidewalls. Through their advanced design features, they provide exceptional image quality needed for use on inverted microscopes and for applications such as high – and super resolution image analysis, microinjection and electro-physiology recording of fluorescent-tagged cells.

The WillCo Wells glass bottom Petri dishes use a special formulated adhesive with a low toxicity that is durable and optically clear. Glass thickness stays within 0.17mm and ra ±0.005mm and permits use of immersion objectives with water, glycerine or oil. The glass bottom is flush with the microscope stage or heating stage to ensure absolute flatness and optimum heat exchange. The optical quality of the glass bottom provides a higher effective numerical aperture (NA) producing brighter high resolution images in all types of microscopy applications.

The 50mm WillCo Wells glass bottom Petri dish features a low rim side wall. The low side wall allows easier access for micromanipulation/microinjection probes. The WillCo Wells Glass Bottom Petri dish is available in two sizes: 35mm diameter and 50mm diameter. Both sizes are available with clear or black side walls.

The 35mm dish will be compatible with most 35mm heaters and perfusion adapters.

The 50mm dishes, ‘Series 5030’ and ‘Series 5040’, have a well of respectively 30mm and 40mm, both providing a large growth area. They have a low access angle and an "easy grip" handling.

PELCO^® Clear Wall Glass Bottom Dishes

35 x 10mm Clear Wall Glass Bottom Dishes

Available in Blister Packs or Sleeves of 20

50 x 7mm Clear Wall Glass Bottom Dishes

Applications:

Live Cell Microscopy
Multi-Photon Laser Scanning Microscopy (MPLSM)
High Level Laser Fluorescence
Fluorescence Lifetime Imaging Microscopy (FLIM)
Fluorescence Resonance Energy Transfer Microscopy (FRETM)
Fluorescence Redistribution After Photobleaching Microscopy (FRAPM)
Epifluorescence Microscopy
Fluorescence Imaging
Immunofluorescence Microscopy
Confocal Microscopy
Confocal Laser Scanning Microscopy (CLSM)
Phase Contrast Microscopy
Polarized Light Microscopy
Differential Interference Contrast
High Resolution Image Analysis
Time Lapsed Photography
Video-Enhanced Microscopy
Infrared imaging
Calcium imaging
Colocalization studies
Green Fluorescent Protein (GFP)
Micromanipulation
Microinjection
Super resolution imaging
Nanoscopy imaging

Advantages:

Much larger effective range, using an objective lens with a short working distance
No birefringence effects because of thin (Class #1.5H / 0.17mm) glass bottom
Low rim/side wall of the dish and a larger effective working distance make micromanipulation and microinjection possible
Higher effective N.A. make brighter images (in e.g.: fluorescence)
Higher effective N.A. make higher resolutions in image analysis
Microscope light passes through the bottom glass in a perpendicular way, glass bottom rests directly on stage
Direct heating from the microscope-stage, because the glass is in direct contact (flush) with stage
In IVF, no more oocyte/embryo transfer is needed from one plastic dish to another, unless different media are being used, saving a lot of time (Do not put in incubator!)
Easy "perfusion" in the dish for continuous in and outflow of solutions, using "perfusion chamber insert", for life cells.
The German glass, borosilicate D263M, is used in microscopy for medical, biological and research work, e.g. in fluorescence microscopy

Fields of Application
Fertility & Infertility	ICSI, spermatid identification, assisted hatching, embryo biopsy, genetics, etc.
(Cell-) Physiology	Patch clamping, ion-imaging, etc.
Pathology	Studies e.g. on the morphology of particles and cells, etc.
Neurology	Axon growth, in and efflux studies of electrolytes, etc.
Cell Biology	Cell-cell communication studies, tissue culture, etc.
Molecular Biology	Studies on genetics (DNA), enzymatic activity, etc.
Pharmacology	Cell research, in and outside the cell on all levels.
Plant Physiology	Root hair growth, micro-parasites on roots, etc.

Cross section view of Black Wall
and Clear Wall Glass Bottom Dishes

Product Number	Dish OD	Aperture ID	Optical Glass OD	Dish ID	Outside Height	Access Angle
14020 14028	35mm	12mm	34mm	27mm	10mm	36°
14022 14030	35mm	22mm	34mm	27mm	10mm	36°
14024 14032	50mm	30mm	49mm	44mm	7mm	17°
14026 14035	50mm	40mm	49mm	44mm	7mm	17°