|
Processing Step
|
Routine Microwave Processing 1
|
Vacuum Microwave
Processing 2
|
Routine Processing
|
|
1. Primary Fixation (aldehyde)
|
.....10 minutes
|
.....6 minutes
|
.....1 hour
|
|
2. Buffer Rinse
|
.....6 minutes
|
.....4 minutes
|
.....0.5 hours
|
|
3. Secondary Fixation (osmium)
|
.....10 minutes
|
.....6 minutes
|
.....1 hour
|
|
4. Dehydration (acetone/ethanol)
|
.....7 minutes
|
.....7 minutes
|
.....2 hours
|
|
5. Resin Infiltration
|
.....50 minutes
|
.....8 minutes
|
.....18 hours
|
|
6. Tissue to Embedding Capsules
|
.....15 minutes
|
.....15 minutes
|
.....0.25 hours
|
|
7. Resin Polymerization
|
.....45-75 minutes
|
.....45-75 minutes
|
.....18 hours
|
|
TOTALS
|
.....~170 minutes
|
.....~120 minutes
|
.....~2,400 minutes
|
1 Giberson, et
al., 1997. J. Vet. Diagn. Invest. 9:61-67.
2 Unpublished work in progress.