Cryo Embedding & Supplies

PolarStat Plus™

Medium viscosity formulation.

PolarStat Plus™ is an exceptional cryo embedding media that works well with an anti-roll plate or may be led off the blade with a brush. It is especially well-suited for Mohs cryostat sectioning.

• Now available in 4 optimal colors to accompany the traditional white formulation.
• This formulation freezes at -10°C.
• Optimal cutting temperature ranges from below-10°C to -40°C.
• Does not produce a powder residue while sectioning at -25°C or colder.
• Works with Auto Cryo Approach in Microm cryostats.
• No residue is left on slides during the staining procedure, thereby eliminating undesirable background staining.

White: SDS (406KB PDF)
Blue: SDS (407KB PDF)
Yellow: SDS (407KB PDF)
Green: SDS (407KB PDF)
Red: SDS (407KB PDF)

Tissue Freezing Medium

OCT Compound for Cryostat Sectioning, Tissue-Tek^®

Golden Nylon Brushes for Cryo Microtomy

Camel Hair Brush

Tissue Marking Dye

Our new tissue marking dyes are formulated to provide very bright and clear identification of microscopic areas of interest and to easily identify the orientation of excised tissue samples. These dyes are readily visible on cut frozen tissue sections. These dyes will not run, bleed, fade or lose intensity from tissue processing and/or during staining procedures in the histology laboratory.

• Permanent tissue marking dyes.
• Colors formulated to define margins without bleeding or fading.
• Durable, bright dyes hold permanently through processing and staining procedures.
• Unique lid design allows for complete removal or they may be conveniently flipped open with little risk of drying.
• Available in 2oz. flip-top bottles in the following colors:
  
  Yellow: SDS (522KB PDF)
  Red: SDS (521KB PDF)
  Green: SDS (514KB PDF)
  Black: SDS (510KB PDF)
  Blue: SDS (522KB PDF)

Applications/intended use:

• Mohs surgery procedures to mark tissue specimens to map and identify the orientation and borders of tissue specimens.
• Pathology grossing of tissue to identify areas of interest and orientation of surgically excised tissue specimens.
• Marking surgical margins.
• Identifying serial segments of large tissues to ensure proper orientation and embedding.
• Identification of multiple pieces of tissues processed in one cassette
• May be used on fresh or fixed tissue.

Note: Pretreatment of tissue to be dyed with #27208, ColorBond™ Mordant may greatly enhance the performance of tissue marking dyes, especially with fatty tissues.

PELCO® Technical Notes, Tissue Marking Dyes (267KB PDF)

Tissue Marking Dye Starter Kit

• Tissue Marking Dye Holding Rack #27213-R
• Five colors of Tissue Marking Dye #27213
• Dye Mordant #27208
• Four variations of dye applicators: 1,000 Cotton Tip #80907; 50 Polypropylene Spade Tip #81536; 50 of each Wide Foam Tip #81442 (1/2" tip), #81444 13/16" round tip)

Tissue Marking Dye Holding Rack

ColorBond™ Tissue Marking Dye Mordant

PELCO Histo/Cyto-Freeze™ Cryo Spray

MSDS (98KB PDF) - -

Hazard Shipping Fees

Cryogen Spray

MSDS (213KB PDF) - -

Hazard Shipping Fees

Cryo Microtome Lubrication Kit

Pen for Frozen Sections: Fro-Tissuer

General Use: Cryo Sectioning
Surfaces: Glass

PELCO® Technical Notes, Fro-Tissuer, Adhesive Pen for Frozen Sections (PDF 479KB)

MSDS (PDF 68KB)

Tissue-Tek^® Cryomolds^®

For use with Tissue-Tek^® II M/C

Tissue-Tek Cryomold^® creates a uniformly shaped, flat-surface specimen blocks with O.C.T. Compound for frozen sectioning. Disposable PVC plastic molds fit Cryobar^® freezing wells. Available in 3 sizes: biopsy (10 x 10 x 5mm), intermediate (15 x 15 x 5mm) and standard (25 x 20 x 5mm) are provided 100 per package.

NEW CryoSette™ Frozen Tissue Storage Container

The CryoSette™ is designed for frozen tissue collection, transport and storage. It can also be used for storing a variety of other specimens in all laboratories. The robust design ensures that tissue morphology will be well preserved.

• Base made of acetyl resin polymer
• Screw cap is made of high density polyethylene
• Offers sample specimen storage in upright/chest freezer and LN₂ vapor phase dewar down to -196°C
• Color code inserts available
• Cardboard label for CryoSette™
• Transparent plastic label cover
• Top writing or cryo label space for labeling
• Maintains the integrity of tissue samples
• Choice of two storage boxes

The 2.5ml container features a wide-mouth opening and a high integrity screw closure. It is designed for a flat bottom and straight sides for easy tissue removal. The specific shape of the CryoSette™ allows its manipulation to be optimized, whether you are using gloves or not. The CryoSette™ efficiently stores tissue samples in -86°C ultra low freezers or in the gas phase of LN₂ in Dewar flasks at -196°C, but not in LN₂ liquid phase.

The CryoSette™ screw closure and base are designed so they will not seize at low temperatures. Rapid visual 1-1/4 turn on/off. Color-coded inserts available in 5 colors.

The CryoSette™ offers two surfaces for handwriting or applying identification labels: an interior surface and a larger space underneath the base. A thin Cardboard I.D. Label (product number 20955-10) and Plastic Transparent Cover (product number 20955-11) are available for the latter. The CryoSette™ is compatible with common tracking methods. The anterior writing surface is improved for ink adherence. Color Coded Identification Inserts (product numbers 20955-5 through 20955-9) are available in 5 colors: blue, red, green, yellow and lilac.

Slanted writing surface with Color Code Insert™	Cardboard I.D. Label and Plastic Transparent Cover

NEW CryoSette™ Storage Rack

• Made of polypropylene
• Compatible with leading frozen sample storage systems
• Thick walls minimizing deformation due to temperature changes
• Can be handled by robotic equipment
• Rack orientation marks on sides
• Latches for locking and unlocking rack lid
• Autoclavable

The Storage Rack is designed to contain 21 frozen tissue CryoSette™ Containers. The box is compact and has an SBS standard footprint to make suitable to several robotic storage systems. These innovative boxes can be stored in low-temperature freezers.

Temperature range: -196°C to 110°C. Suitable for cryogenic storage, but only in LN₂ vapor phase.
Dimensions: 127.76mm x 85.48mm x 41.6mmH

NEW CryoSette™ CryoStore Container

The CryoSette™ CryoStore box can accommodate up to 40 CryoSette™ tissue containers in a space as small as 133 x 133 x 52mm H.  Made of extra strong polycarbonate, the durable cryogenic storage box is designed to be used at temperatures between -196°C and +121°C and is autoclavable at 120°C, 15 psig (1 bar) for 20 minutes. Only use in LN₂ vapor phase.

The frosted cover allows the user to write directly on top of the box, and is keyed to the base to prevent misalignment.

Cryovial^® Tubes

• Specially formulated polypropylene
• Storage temperature as low as -196°C (gas phase)
• Only 1-1/4 turn required for cap seal Sterilized by gamma irradiation/packaged in tamper-proof resealable bags
• Certified RNase, DNase, Pyrogen and DNA-free
• Autoclavable
• White labeling area on each tube
• Certain sizes in round bottom (rb) and/or free-standing (fs)
• Only round bottom tubes can be centrifuged (17,000g)
• IVD approved
• Capinsert™ color coded inserts and Cryovial® Cane available

Cryovial^® Tubes

• Specially formulated polypropylene
• Storage temperature as low as -196°C (gas phase)
• Only 1-1/4 turn required for cap seal Sterilized by gamma irradiation/packaged in tamper-proof resealable bags
• Certified RNase, DNase, Pyrogen and DNA-free
• Autoclavable
• White labeling area on each tube
• Certain sizes in round bottom (rb) and/or free-standing (fs)
• Only round bottom tubes can be centrifuged (17,000g)
• IVD approved
• Capinsert™ color coded inserts and Cryovial® Cane available

Cryovial^® Tubes

• Specially formulated polypropylene
• Storage temperature as low as -196°C (gas phase)
• Only 1-1/4 turn required for cap seal Sterilized by gamma irradiation/packaged in tamper-proof resealable bags
• Certified RNase, DNase, Pyrogen and DNA-free
• Autoclavable
• White labeling area on each tube
• Certain sizes in round bottom (rb) and/or free-standing (fs)
• Only round bottom tubes can be centrifuged (17,000g)
• IVD approved
• Cap contains long skirt for one-handed sterile technique
• Capinsert™ color coded inserts and Cryovial® Cane available

Cryovial^® Tubes

• Specially formulated polypropylene
• Storage temperature as low as -196°C (gas phase)
• Only 1-1/4 turn required for cap seal Sterilized by gamma irradiation/packaged in tamper-proof resealable bags
• Certified RNase, DNase, Pyrogen and DNA-free
• Autoclavable
• White labeling area on each tube
• Certain sizes in round bottom (rb) and/or free-standing (fs)
• Only round bottom tubes can be centrifuged (17,000g)
• IVD approved
• Cap contains long skirt for one-handed sterile technique
• Capinsert™ color coded inserts and Cryovial® Cane available

Cryovial^® Tubes

• Specially formulated polypropylene
• Storage temperature as low as -196°C (gas phase)
• Only 1-1/4 turn required for cap seal Sterilized by gamma irradiation/packaged in tamper-proof resealable bags
• Certified RNase, DNase, Pyrogen and DNA-free
• Autoclavable
• White labeling area on each tube
• Certain sizes in round bottom (rb) and/or free-standing (fs)
• Only round bottom tubes can be centrifuged (17,000g)
• IVD approved
• Cap features long skirt for one-handed sterile technique
• Capinsert™ color coded inserts and Cryovial® Cane available

Capinsert^™ for Cryovial^® Tubes

Polypropylene inserts which fit directly into the caps of the Cryovial^® Tubes for unique color identification.

Available in 5 individual colors or a case of assorted colors.

Cane for Cryovial^® Tubes

Aluminum cane specifically designed for storage of Cryovial^® Tubes. Each Cane is engineered to hold up to 5 of the 1.2 or 2 ml Cryovial^® Tubes.

Canes are for storage placement in liquid nitrogen dewars.

Cryostore^™ Containers

Cryo Compatible Slide Boxes

The unique and durable polycarbonate construction of the Cryo Compatible Slide Box, protects your 3 x 1" (75 x 25mm) slides in ultra-low temperature ranges to -80°C (-112°F). Available in a 100-slide and a 25-slide capacity box with grooved slots to prevent slide to slide contact. The Cryo Compatible Slide Boxes offer a permanent numbered index sheet in the lid that corresponds to the numbering on the temperature-resistant foam lining in the base. These features provide ideal inventory maintenance ability. The boxes are stackable and can be autoclaved.

• Durable polycarbonate construction for ultra-low temperatures
• Temperature resistant foam liner and grooved slots
• 100 slide capacity 3 x 1" (75 x 25mm)
• Dimensions: 208 x 175 x 34mm (8.19 x 6.89 x 1.34")
• 25 slide capacity 3 x 1" (75 x 25mm)
• Dimensions: 141 x 88 x 35mm (5.5 x 3.46 x 1.38")
• Autoclavable and stackable