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TEM Grids Overview
PELCO® Silicon Nitride Support Films for TEM
Hydrophobic/Hydrophilic SiN Support Films
PELCO® Silicon Dioxide Support Films for TEM
Silicon Aperture Frames (without support films)
Silicon Nitride Coated 3mm Disks (blanks)

NEW PELCO® Silicon Nitride
Support Film for CLEM


Silicon Nitride Membrane for CLEM   Silicon Nitride Membrane for CLEM
3.0mm major diameter with two parallel
1mm flats for repeatable alignment
  FE-SEM image of flat


The silicon nitride support film on a silicon disc is specially truncated with two parallel flats, which act as a fiducial for Correlative Light & Electron Microscopy (CLEM) experiments. The discs have a wide range of apertures and can be purchased with or without an electron transparent silicon nitride support film. The grids are 200µm thick, and are 3mm in diameter, but have two 1mm long flat edges on both sides. The films come in 4 different thicknesses, 8nm, 15nm, 50nm, and 200nm, as well as no support film.

  • The durability of the silicon nitride support film allows cells to be cultured directly onto them.
  • Two grids can be glued together to construct a sealed, liquid-filled chamber allowing the samples to remain in fluid while imaging in the electron microscope. Sealing can be done with either a fast curing epoxy glue #14420 or M-Bond® 610 #16039
  • The discs can be also be imaged in the light microscope, and because of the parallel flats on both sides of the disc, the orientation of the sample can be maintained throughout the different imaging modes.

Silicon Nitride Membrane for CLEM
1 x 1mm 0.75 x 0.75mm 0.5 x 0.5mm 0.25 x 0.25mm 0.5 x 1.5mm 0.1 x 1.5mm 0.1 x 0.1mm


Prod # Description Unit Price Order / Quote
21518-10 PELCO® 50nm Silicon Nitride Support Film, with 1.0 x 1.0mm Window, for CLEM pkg/10 $168.00
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21501CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.75 x 0.75mm Window, for CLEM pkg/10 168.00
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21504CL-10 PELCO® 50nm Silicon Nitride Support Film, with 1.5 x 0.5mm Window, for CLEM pkg/10 168.00
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21505CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.25 x 0.25mm Window, for CLEM pkg/10 165.00
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21508CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.1 x 1.5mm (2) Windows, for CLEM pkg/10 168.00
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21509CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.1 x 0.1mm (9) Windows, for CLEM pkg/10 168.00
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21520CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.5 x 0.5mm Window, for CLEM pkg/10 125.00
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21521CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.75 x 0.75mm Window, for CLEM pkg/10 125.00
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21529CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.1 x 0.1mm (9) Windows, for CLEM pkg/10 125.00
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