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TEM Grids Overview
PELCO® Silicon Nitride Support Films for TEM
Hydrophobic/Hydrophilic SiN Support Films
PELCO® Silicon Dioxide Support Films for TEM
Silicon Aperture Frames (without support films)
Silicon Nitride Coated 3mm Disks (blanks)

NEW PELCO® CLEM Silicon Support
Grids with Silicon Nitride Membranes


Silicon Nitride Membrane for CLEM   Silicon Nitride Membrane for CLEM
3.0mm major diameter with two parallel
1mm flats for repeatable alignment
  FE-SEM image of flat


These silicon support grids with silicon nitride membranes have frames that are specially truncated with two parallel flats, which act as an aid for specimen alignment in Correlative Light & Electron Microscopy (CLEM) experiments. The CLEM grids are 200µm thick and 3mm in diameter, with two 1mm-long parallel flat edges on both sides. The silicon nitride films are available in four different thicknesses: 15 nm 50 nm, 100 nm and 200 nm, with a variety of aperture sizes from 0.1 x 0.1mm to 1 x 1mm.

  • The durability of the silicon nitride support film allows cells to be cultured directly on them.
  • Two CLEM grids can be glued together to construct a sealed, liquid-filled chamber, allowing the samples to remain in fluid while imaging in an electron microscope. Sealing can be done with either a fast-curing epoxy glue #14420 or M-Bond® 610 #16039
  • The presence of the parallel flats on both sides of the CLEM grid allows the orientation and alignment of the sample to be maintained when using different imaging techniques.
Silicon Nitride Membrane for CLEM
1 x 1mm 0.75 x 0.75mm 0.5 x 0.5mm 0.25 x 0.25mm 0.5 x 1.5mm 0.1 x 1.5mm 0.1 x 0.1mm



Prod # Description Unit Price Order / Quote
21560CL-10 PELCO® 15nm Silicon Nitride Support Film, with 0.25 x 0.25mm Window, for CLEM pkg/10 $245.00
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21568CL-10 PELCO® 15nm Silicon Nitride Support Film, with 0.1 x 1.5mm (2) Windows, for CLEM pkg/10 245.00
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21569CL-10 PELCO® 15nm Silicon Nitride Support Film, with 0.1 x 0.1mm (9) Windows, for CLEM pkg/10 245.00
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21518-10 PELCO® 50nm Silicon Nitride Support Film, with 1.0 x 1.0mm Window, for CLEM pkg/10 168.00
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21500CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.5 x 0.5mm Window, for CLEM pkg/10 168.00
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21501CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.75 x 0.75mm Window, for CLEM pkg/10 168.00
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21504CL-10 PELCO® 50nm Silicon Nitride Support Film, with 1.5 x 0.5mm Window, for CLEM pkg/10 168.00
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21505CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.25 x 0.25mm Window, for CLEM pkg/10 165.00
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21508CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.1 x 1.5mm (2) Windows, for CLEM pkg/10 168.00
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21509CL-10 PELCO® 50nm Silicon Nitride Support Film, with 0.1 x 0.1mm (9) Windows, for CLEM pkg/10 168.00
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21506CL-10 PELCO® 100nm Silicon Nitride Support Film, with 0.5 x 0.5 mm Window, for CLEM pkg/10 117.00
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21516CL-10 PELCO® 100nm Silicon Nitride Support Film, with 0.75 x 0.75 mm Window, for CLEM pkg/10 133.00
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21514CL-10 PELCO® 100nm Silicon Nitride Support Film, with 1.0 x 1.0mm Window, for CLEM pkg/10 133.00
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21511CL-10 PELCO® 100nm Silicon Nitride Support Film, with 1.5 x 0.5mm Window, for CLEM pkg/10 133.00
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21507CL-10 PELCO® 100nm Silicon Nitride Support Film, with 0.25 x 0.25mm Window, for CLEM pkg/10 117.00
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21512CL-10 PELCO® 100nm Silicon Nitride Support Film, with 0.1 x 1.5mm (2) Windows, for CLEM pkg/10 117.00
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21513CL-10 PELCO® 100nm Silicon Nitride Support Film, with 0.1 x 0.1mm (9) Windows, for CLEM pkg/10 117.00
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21520CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.5 x 0.5mm Window, for CLEM pkg/10 125.00
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21521CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.75 x 0.75mm Window, for CLEM pkg/10 125.00
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21529CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.1 x 0.1mm (9) Windows, for CLEM pkg/10 125.00
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21524CL-10 PELCO® 200nm Silicon Nitride Support Film, with 1.5 x 0.5mm Window, for CLEM pkg/10 135.00
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21522CL-10 PELCO® 200nm Silicon Nitride Support Film, with 1.0 x 1.0mm Window, for CLEM pkg/10 130.00
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21525CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.25 x 0.25mm Window, for CLEM pkg/10 125.00
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21528CL-10 PELCO® 200nm Silicon Nitride Support Film, with 0.1 x 1.5mm (2) Windows, for CLEM pkg/10 125.00
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PELCO® CLEM Silicon Support Grids with Apertures

The edges of these silicon frames are specially truncated with two parallel flats, which act as an aid for specimen alignment in Correlative Light & Electron Microscopy (CLEM) experiments. These CLEM grids without a silicon nitride membrane are available in a variety of different size apertures and patterns. The CLEM grids are 200 µm thick and 3 mm in diameter with two 1 mm-long parallel flat edges on both sides. The grids have a 50 nm silicon nitride layer on the back side with bare silicon on the front side. Use of the parallel flats on both sides of the CLEM grid allows the orientation and alignment of the sample to be maintained when using different imaging techniques.

Prod # Description Unit Price Order / Quote
21540CL-10 PELCO® CLEM Silicon Support Grids with Apertures 0.5 x 0.5mm (no support film) pkg/10 $125.00
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21541CL-10 PELCO® CLEM Silicon Support Grids with Apertures 1.0 x 1.0mm (no support film) pkg/10 125.00
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21542CL-10 PELCO® CLEM Silicon Support Grids with Apertures 0.5 x 1.5mm (no support film) pkg/10 125.00
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21543CL-10 PELCO® CLEM Silicon Support Grids with Apertures 0.75 x 0.75mm (no support film) pkg/10 125.00
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21545CL-10 PELCO® CLEM Silicon Support Grids with Apertures 0.25 x 0.25mm (no support film) pkg/10 125.00
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21548CL-10 PELCO® CLEM Silicon Support Grids with Apertures 0.1 x 1.5 mm (2) (no support film) pkg/10 125.00
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21549CL-10 PELCO®CLEM Silicon Support Grids with Apertures 0.1 x 0.1mm (9) (no support film) pkg/10 125.00
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