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PELCO easiGlow™ Glow Discharge Cleaning System

Optimized for cleaning TEM grids

NEW PELCO easiGlow Cryo-EM Glow Discharge Set
easiGlow™ Glow Discharge Cleaning System

For one of the most common glow discharge applications, making TEM support films or grids hydrophilic using air, the PELCO easiGlow™ uses an automated and quick cycle with fully selectable parameters. The microprocessor controlled system also offers full manual control for all parameters and an advanced protocol programming feature for custom designed glow discharge applications. Parameters and protocols can be stored to facilitate consistent glow discharge results. Open the shipping box, plug it in, and you can start.

Full List of Specifications

The PELCO easiGlow™ Glow Discharge Cleaning System is a compact, quick and easy to use standalone system. It is primarily designed for cleaning TEM grids and hydrophilization of TEM carbon support films, which have the tendency to be hydrophobic. A glow discharge treatment with air will make a carbon film surface negatively charged (hydrophilic) which allows aqueous solutions to spread easily. Manufactured in USA.

With increasingly demanding imaging applications, using clean and consistent quality TEM grids and supports has become more important than ever. Glow discharge treatment of TEM grids removes adsorbed hydro carbons, cleaning them while making them hydrophilic. The PELCO easiGlow™ is an affordable and easy to operate glow discharge system for any TEM lab.

The practical chamber size with its newly designed glow discharge head and adjustable stage with glass slide holder allows for easy loading and fast turnaround times. The system is fully microprocessor controlled and includes an intelligent touch screen device for control and display of operation parameters. The PELCO easiGlow™ supports both hydrophilic and hydrophobic treatment with either negative or positive charge and includes two separately controlled gas inlets. The required vacuum level is set by an electronically controlled precision proportional valve. This novel approach eliminates manual setting with a needle valve. A gentle venting procedure ensures that the TEM grids are not disturbed when the system is vented.

Glow Discharge Methods supported:

Surface Condition Charge Atmosphere
Hydrophilic Negative Air
Hydrophilic Positive Air (with subsequent magnesium Acetate treatment)
Hydrophobic Positive Amylamine (Pentylamine)
Hydrophobic Negative Methanol

Reference for glow discharge and use of amylamine to obtain Hydrophobic; positively charged surfaces: Hayat, M.A.2000. Principles and Techniques of Electron Microscopy Biological Applications, page 237-241. Cambridge University Press. ISBN 052163287

Reference for glow discharge and use of subsequent Magnesium Acetate treatment to obtain Hydrophylic; charged surfaces: Portmann, R., and Koller, Th. 1976. The divalent cation method for protein-free spreading of nucleic acid molecules. In Proceedings of the 6th European Congress on Electron Microscopy, Vol.2, p546.
Optimum Pressure Graph
Efficient glow discharge performance for the PELCO easiGlow™ with optimum pressure at given distance from sample to glow discharge head.

Advantages of the PELCO easiGlow™ System

Specifications of the PELCO easiGlow™ Glow Discharge System

Plasma Current 0-30 mA
HV Power supply 30W Continuous Rating
Glow Discharge Head Polarity User Selectable positive or negative
Process Platform Ø75mm with recess for 25 x 75mm slide
Glow Discharge Platform Height Control 1-25 mm
Preprocess Hold Timer 0-14411 sec.
Process Timer 0-900 sec.
Chamber Size Ø120 x 100mm H with sealed Delrin® top plate (inside Ø106 x 90mm)
Process/Vent gas inlets 2 each Ø6 mm barbed, individually user selectable for process, purge or vent
High Voltage Vacuum Interlocks Dual hardware and software interlocked
Vacuum Control Pirani Gauge, ranging from atmosphere to 0.01 mbar
Working Vacuum Range 1.1 - 0.20 mbar for optimal glow
System Control / Display 3" Touch Panel with LED backlight and 5 function keys
Operation Modes Auto Programmed (4 user programmed protocols) Manual/Diagnostics
Data Logging Capabilities Step #, Vacuum (mbar), Current (mA), Voltage (V), and polarity, once per second
Instrument Size 305 L x 292 D x 230mm H (12" x 11-1/2" x 9 ")
Weight 6.26 kg (13.8 lbs.)
GD4 Pump (optional) 337 L x 138 D x 244mm H (13.3" x 5.4" x 9.6 ")
Weight 11 kg (24 lbs.)
Laboratory Footprint with optional pump 381 W x 292 D x 330mm H (15" x 11-1/2" x 13 ")
Power Requirements 120V 60Hz, 15A or 230V 50Hz, 10A Service
Instrument Power Rating 100-240VAC 60/50Hz 60W Plus Pump, IEC Inlet
Optional Pump Power Rating 120/230V 60/50Hz 370 W (1/2 hp)
Vacuum Pump Minimum requirements 2.5 m3/hr, 41 l/m, 1.65 CFM
0.03 mbar Ultimate Vacuum
Vacuum Pump Power Outlet on Instrument 8A max IEC Outlet
Vacuum Inlet KF 16 flange
Data Logging Capabilities Step #, Vacuum (mbar), Current (mA), Voltage (V), and polarity, once per second
Communication Port USB Outlet

Dual Chamber PELCO easiGlow™ System

For those labs which need both hydrophilic and hydrophobic surface treatments on a regular base, a dual glow discharge system is available to avoid cross contamination of the glow discharge chambers. Hydrophobic surfaces are preferable when organic solvents are used.
Glow Discharge Cleaning System, dual

NEW PELCO easiGlow™ Cryo-EM Glow Discharge Set

For easier ordering, we have created a bundle including all items needed for a typical glow discharge setup in a cryo-EM lab. This convenient set includes, in addition to a PELCO easiGlow™ unit, a specially matched vacuum pump with all accessories, a rubber gasket to protect the glass chamber edge, as well as one PELCO® Grid Holder Block, a handy alternative to putting your grids on glass slides. The Grid Holder Block is included in the bundle free of charge, a savings of $60.

Glow Discharge Cleaning System, dual

Ordering Information for PELCO easiGlow™

Prod # Description Unit Price Order / Quote
91000S NEW PELCO easiGlow™ Cryo-EM Glow Discharge Set each P.O.R.
91000 PELCO easiGlow™ Glow Discharge Unit each P.O.R.
91002 Dual Chamber PELCO easiGlow™ Glow Discharge System each P.O.R.
91050 Replacement Glass Chamber with sealed top plate each 305.50
91000-2 Replacement Touch Screen Stylus each 7.60
91051 O-ring for Glow Discharge Chamber pkg/2 8.50
91053 Protective L-gasket for 91050 each 76.80

Since the optimized vacuum level for glow discharge procedures is in the range of 0.45 to 1.1 mbar, we offer the affordable GD4 dual stage rotary vacuum pumping system, designed specifically for this pressure range.

GD4 Rotary Vacuum Pump for PELCO easiGlow™

Pumping speed 4.3m3/hr, 72 l/min, 2.5 CFM @ 60Hz
3.6m3/hr, 60 l/min, 2.1 CFM @ 50Hz
Ultimate vacuum 1 x 10-2 MBAR
Vacuum connection KF/NW 16
Exhaust connection KF/NW 16
Oil capacity 300cc (included)
Operating temperature 5 - 40°C (41 - 104°F)
Dimensions 337 x 138 x 244mm
13.3" x 5.4" x 9.6" (L x W x H)
Weight 11kg / 24 lbs.
Power rating 110 - 115V 60 Hz, 3.2A, 0.37kW
220 - 250V, 50/60 Hz, 1.6A , 0.37kW

GD8 Vacuum Pump

Prod # Description Unit Price Order / Quote
91040 GD4 Vacuum Pumping System, 4.3m³/hr incl. Connection Kit, 115V, 60Hz each $990.00
91040-230 GD4 Vacuum Pumping System, 3.6m³/hr incl. Connection Kit, 230V, 50/60Hz each 990.00
91042 GD4 Vacuum System, 4.3m³/hr incl. Connection Kits For Dual Chamber Glow Discharge System, 115V, 60Hz each 1960.00
91042-230 GD4 Vacuum System, 3.6m³/hr incl. Connection Kits For Dual Chamber Glow Discharge System, 230V, 50/60Hz each 1960.00
9609 Exhaust filter for GD4 or GD7 Rotary Vacuum Pump each 69.70
891-31 PELCO® Ultragrade 19 Vacuum Pump Oil for GD4, GD7 and GD8 Pumps, 1 ltr each 13.25
91010-16 PELCO easiGlow™ Vacuum Connection Kit to KF16 Pump inlet each 147.80
91010-25 PELCO easiGlow™ Vacuum Connection Kit to KF25 Pump inlet each 198.65
12938-3 Polypropylene Funnels, set of three with 2, 4 and 8 oz each 8.25
808-16 Cotton Shop Cleaning Towels, Blue, 14" x 14" pkg/10 6.30

891-31 SDS (93KB PDF)

PELCO TEM Grid Holder Blocks

PELCO® TEM Grid Holder Blocks

Two sizes available: 25 TEM grids in 5 x 5 matrix and 81 TEM grids in a 9 x 9 matrix.

More information on PELCO® TEM Grid Holder Blocks

Prod # Description Unit Price Order / Quote
16820-25 PELCO® TEM Grid Holder Block for 25 TEM grids each $60.00
16820-81 PELCO® TEM Grid Holder Block for 81 TEM grids each 80.00
Clamping Grid Holder Block

PELCO® Clamping TEM Grid Holder Block

Holds 45 grids securely clamped between two plates.

More information on PELCO® Clamping TEM Grid Holder Block

Prod # Description Unit Price Order / Quote
16830-45 PELCO® Clamping TEM Grid Holder Block for 45 TEM grids each $245.00