Prod #
| Description
| Unit
| Price
| Order / Quote
|
Citifluor Antifadent (AF) Solutions
|
19470
| AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 25ml
| each
| $150.00
|
|
19470-1
| AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 100ml
| each
| 171.00
|
|
I - SDS (46KB PDF)
|
19472
| AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 25ml
| each
| 90.00
|
|
19472-1
| AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 100ml
| each
| 204.00
|
|
I - SDS (110KB PDF) Glycerol solution, additives.
|
19474
| AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 25ml
| each
| 101.00
|
|
19474-1
| AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 100ml
| each
| 228.00
|
|
I - SDS (106KB PDF)
|
19476-A
| Citifluor non-fluorescent Immersion Oil, AF87, 10ml
| each
| 80.00
|
|
I - SDS (47KB PDF)
|
19476
| AF-4, Glycerol solution containing n-propyl gallate for use with labelled tissue sections and dead cells, 10ml
| each
| 98.00
|
|
19490
| AF-100, Phosphate-buffered saline solution containing antifadent specially prepared for use with UVM23 solution where fluorochrome fading is a problem, 5ml
| each
| 20.00
|
|
I - SDS (47KB PDF)
|
19491
| AF-200, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used with other fluorochromes such as fluorescein, Alexa dyes and Hoescht dye, 10ml
| each
| 112.00
|
|
I - SDS (315KB PDF)
|
19492
| AF-300, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used where fluorescein is the staining material. The solution can also be used with other fluorochromes such as rhodamines, etc., 10ml
| each
| 85.00
|
|
I - SDS (111KB PDF)
|
19493
| CFM1, Glycerol-PBS ref. index similar to glass, 10ml
| each
| 57.00
|
|
I - SDS (109KB PDF)
|
19494
| CFM1, Glycerol-PBS w/AF, ref. index similar to glass, 10ml
| each
| 98.00
|
|
I - SDS (pdf 48KB)
|
19495
| CFM2, Glycerol-TRIS buffer, ref. index similar to glass, 10ml
| each
| 60.00
|
|
I - SDS (pdf 109KB)
|
19496
| CFM3, Glycerol-Phosphate & AF, ref. index similar to glass, 10ml
| each
| 86.00
|
|
I - SDS (111KB PDF)
|
UV Mount Media (UVM)
|
19497
| UVM1, Methacrylate based solution, UV curable, gives clear film, 10ml
| each
| 80.00
|
|
I - MSDS (69KB PDF)
|
19498
| UVM2, Methacrylate based solution, contains anti-bleaching agent, 10ml
| each
| 77.90
|
|
I - MSDS 90KB PDF)
|
Solid Mountants
|
19469
| Citifluor Solid Mountant Kit is comprised of PVA solution, 25ml and Citifluor antifadent in buffer, 5ml. The components can be mixed as required, applied to the specimen and allowed to dry to give a clear film, 30ml
| each
| 142.00
|
|
C - SDS for Citifluor AF100 Antifadent Solution, 25ml (46KB PDF)
|
U - SDS for Citifluor CFPVOH Solution, 5ml (44KB PDF)
|
Contains anti-fading solution. Suitable for preventing fading of dyes such as DAPI which have the tendency to disperse in glycerol based mounting solutions.
|
|
Resin with initiator (BPO) packaged separately. Accelerator #18185 can be used for cold-cure (room temperature).
Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below
60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR® as cover (#10506 or #10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer
polyhydroxylated aromatic acrylic resin (see PTFE Molds).
Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that
Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in
a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found
a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions
could be seen on the complimentary surface. Epon sections show relatively smooth surfaces.18 Micrographs appear
similar to freeze-etched images but scaled to nm instead of µm. Viscosity ~= 8cP
18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470
Landmark Paper, Freeze-Fracture
Steere RL: Electron microscopy of structural detail in frozen
biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957
Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987.
Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods,
and Applications, V. 2, (Hayat MA, ed)
PELCO® Technical Notes, LR White Embedding Medium,
Prod. Nos. 18181 & 18182 |
 |
PELCO® Pro Cyanoacrylate Debonder
The PELCO® Pro Cyanoacrylate Debonder destabilizes and debonds cured cyanoacrylates.
Useful for releasing specimens from holders, cleaning cyanoacrylates from surfaces and removing
cured cyanoacylates from fingers. Works on all brands of cyanoacrylate glue (Loctite, Henkel,
Permabond, 3M, PELCO®, Aron Alpha, etc.). The debonder is also a solvent, please check
before using on plastic surfaces / samples and avoid repeated skin contact. Supplied in 20ml bottle
with dispenser tip.
|
Prod #
| Description
| Unit
| Price
| Order / Quote
|
19920
| PELCO® Pro Cyanoacrylate Debonder, 20ml
| each
| $7.00
|
|
|
The shelf life of cyanoacrylate glues (and epoxies) can be extended by storing them
at lower temperatures; -20°C. At lower temperatures, the adhesive becomes very
viscous and the speed of chemical reactions is practically reduced to zero. It will
keep the adhesive stable for years. Before use, let the adhesive thaw with the container
sealed to avoid condensation into the adhesive.
Cyanoacrylate Brand Cross Reference and Technical Data
|