
Polystyrene Latex Spheres
Used as a magnification test specimen in TEM and SEM, but is also employed in particle counting devices
as a size check and focus aid. Covers µm to nm range. Offered in a 10ml size, at 0.1% weight by volume.
Specific gravity is 1.05 g/ml. MSDS (89KB PDF)
Sphere Size, Size Deviation, Sphere Density Table 
Prod. No. 
Nom Size (µm) 
Size Uniformity (C.V.) 
Approx. Spheres/cc 
61003 
0.03 
≤30% 
6.74E+13 
61008 
0.08 
≤18% 
3.55E+12 
61010 
0.09 
≤15% 
2.50E+12 
61014 
0.17 
≤5% 
3.70E+11 
61017 
0.26 
≤3% 
1.04E+11 
61020 
0.30 
≤3% 
6.74E+10 
61030 
0.49 
≤3% 
1.55E+10 
61038 
1.00 
≤3% 
1.82E+09 
Note: actual size will appear on product label

Prod #
 Description
 Unit
 Price
 Order / Quote

61003
 Latex Spheres, 0.03µm, nom
 10ml
 $15.85


61008
 Latex Spheres, 0.08µm, nom
 10ml
 15.85


61010
 Latex Spheres, 0.09µm, nom
 10ml
 15.85


61014
 Latex Spheres, 0.17µm, nom
 10ml
 15.85


61017
 Latex Spheres, 0.26µm, nom
 10ml
 15.85


61020
 Latex Spheres, 0.30µm, nom
 10ml
 15.85


61030
 Latex Spheres, 0.49µm, nom
 10ml
 15.85


61038
 Latex Spheres, 1.00µm, nom
 10ml
 15.85


Set of latex spheres, 0.09, 0.30, and 0.49 µm nominal diameters.

610SET
 Latex Spheres, set of 3
 3x10ml
 42.50



Certified Particle Size Polystyrene  Nanosphere Size Standards
These Nanosphere Size Standards are highly uniform polystyrene spheres calibrated in billionths
of a meter (nanometers) with NIST traceable methodology. One nanometer is 0.001 micrometer
(µm) or 10 Angstrom units.
Nanosphere Size Standards are packaged as aqueous suspensions in 15 milliliter (ml) droppertipped
bottles. The concentrations are optimized for ease of dispersion and colloidal stability. The spheres
have a density of 1.05 g/cm^{3} and an index of refraction of 1.59 @ 589nm (23°C).
Methods used to calibrate the diameter of the Nanospheres include adaptions of photon correlation
spectroscopy (PCS) and transmission electron microscopy (TEM). PCS uses scattered laser light to
correlate the diameter of suspended particles with their Brownian motion. PCS is also referred to
as quasielastic light scattering or dynamic light scattering.
Nanopshere Size Standards are ideal for the calibration of electron and atomic force microscopes.
They are also used in laser lightscattering studies and colloidal systems research. The 20 to 900nm
range of diameters is convenient for checking the sizes of bacteria, viruses, ribosomes and
subcellular components. MSDS
(88KB PDF)


Sphere Size and Size Deviation Table 
Prod. No. 
Nom Diameter 
Certified Mean Diameter 
Size Distribution Std Dev. & C.V. 
Solids Content 
61050 
20nm 
21nm ±1.5nm 
not determined 
1% 
61053 
50nm 
46nm ±2.0nm 
7.2nm (15.7%) 
1% 
61056 
80nm 
81nm ±2.7nm 
5.8nm (7.2%) 
1% 
61058 
100nm 
97nm ±3nm 
4.5nm (4.6%) 
1% 
61060 
150nm 
151nm ±4nm 
3.2nm (2.1%) 
1% 
61061 
200nm 
200nm ±6nm 
3.4nm (1.7%) 
1% 
61063 
240nm 
240nm ±6nm 
3.7nm (1.5%) 
1% 
61066 
350nm 
350nm ±7nm 
4.7nm (1.3%) 
1% 
61069 
500nm 
491nm ±4nm 
6.3nm (1.3%) 
1% 
61073 
600nm 
596nm ±6nm 
7.7nm (1.3%) 
1% 
61076 
900nm 
903nm ±9nm 
9.3nm (1.0%) 
1% 

Prod #
 Description
 Unit
 Price
 Order / Quote

61050
 Certified Latex Nanospheres, 20nm
 15ml
 $468.50


61053
 Certified Latex Nanospheres, 50nm
 15ml
 481.80


61056
 Certified Latex Nanospheres, 80nm
 15ml
 481.80


61058
 Certified Latex Nanospheres, 100nm
 15ml
 481.80


61060
 Certified Latex Nanospheres, 150nm
 15ml
 481.80


61061
 Certified Latex Nanospheres, 200nm
 15ml
 481.80


61063
 Certified Latex Nanospheres, 240nm
 15ml
 481.80


61066
 Certified Latex Nanospheres, 350nm
 15ml
 481.80


61069
 Certified Latex Nanospheres, 500nm
 15ml
 481.80


61073
 Certified Latex Nanospheres, 600nm
 15ml
 481.80


61076
 Certified Latex Nanospheres, 900nm
 15ml
 481.80


