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PELCO® Support Films of Formvar, Formvar/Carbon, Carbon, and Silicon Monoxide are available on the following 3.05mm O.D. grids: 0.4 x 2mm single slot Cu, 75 mesh Cu, 200 mesh Cu or Ni, 300 mesh Cu or Au and 400 mesh Cu. |
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Support films on the finer mesh grids can withstand considerable handling during
specimen preparation. Those on slot and 75 mesh grids require more gentle handling
and are ideal for those applications requiring large viewing areas without grid bar
interference. Support Film Product Note, How Material is Layered on the Grid. Consult "Applications Guide" for suggestions on support film choice.
Thickness of Support Films |
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1. Formvar: A film of pure Formvar, with no stabilizing coating. These films are useful for your customized coating of stabilizing material or for the support of thin sections. |
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 200 Mesh  0.4 x 2mm Slot |
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2. Formvar, Stabilized with Carbon: A Formvar film covered with a "light" layer of carbon. The heat and electrical conducting properties of carbon help to stabilize the Formvar films when exposed to the electron beam. This is a resilient, all-purpose specimen support film, ideal for mounting thin sections and for applications using lower ranges of magnification. The 200M-TH grids (Prod. No. 01803 and 01803-F) are thicker and more rigid than normal and useful for many applications - particularly where a lot of grid handling is involved. |
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 75 Mesh 200 Mesh  300 Mesh  400 Mesh 0.4 x 2mm Slot |
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3. Carbon Type-B: A Formvar film coated with a “heavier” layer of carbon. This is the strongest and most versatile support film we produce. It is stable in the EM under all operating conditions including high magnification with high beam intensity. The films can withstand vigorous specimen preparation techniques. If the carbon surface is hydrophobic, specimen suspensions can be applied to the Formvar surface. |
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200 Mesh 300 Mesh 400 Mesh |
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4. Carbon Type-A: Carbon support films with a removable Formvar on the opposite side of the grid. When the Formvar is removed, by dipping in solvent, a pure carbon film remains. (Note: The Ultrathin Carbon Film on a Holey Carbon Support Film, Prod. No. 01824, has no Formvar backing.) These films are stable under all EM operating conditions and are for use where the presence of a Formvar layer cannot be tolerated. Pure carbon films tend to be more delicate than those with a Formvar backing and require more delicate handling during specimen preparation than most other support films. (a) Carbon Type-A: Carbon support film of 15 to 25nm thickness. |
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300 Mesh |
PELCO® Technical Notes, Removal of Formvar Film From Carbon Type-A and Silicon Monoxide Type-A Support Films, Product Numbers 01820, 21, 22, 22F, 29, 29F, 1890, 90F (PDF 81KB) |
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(b) Ultrathin Carbon Type-A: Carbon support film of approximately 3nm thickness. |
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400 Mesh |
PELCO® Technical Notes, Removal of Formvar Film From Carbon Type-A and Silicon Monoxide Type-A Support Films, Product Numbers 01820, 21, 22, 22F, 29, 29F, 1890, 90F (PDF 81KB) |
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(c) Ultrathin Carbon Film on a Holey Carbon Support Film: Pure carbon support films with no Formvar backing. This new product has an even thinner carbon film which is mounted on a carbon holey film. The carbon support layer lying over the holes is less than 3nm in thickness and is the thinnest support film available. It is particularly useful for high resolution microscopy of low contrast particles and is also ideal for use with the Energy Filtering TEM. |
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400Mesh |
PELCO® Technical Note, Ultrathin Carbon Film Supported by a Lacey Carbon Film on a 400 Mesh Copper Grid (PDF File) |
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5. Silicon Monoxide: Silicon monoxide produces a highly resilient support film which can withstand vigorous specimen preparation techniques. It has low background contrast, is stable under the electron beam and is less hydrophobic than carbon. We offer two types of silicon monoxide support films: (a) Formvar Stabilized with Silicon Monoxide: A Formvar film coated with a “light” layer of silicon monoxide. |
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200 Mesh |
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(b) Silicon Monoxide Type-A: Silicon monoxide with a removable Formvar backing on the opposite side of the grid. When the Formvar is removed, by dipping in solvent, a pure Silicon Monoxide film remains. These films are stable under all EM operating conditions and for use where the presence of Formvar can not be tolerated. Pure Silicon Monoxide is more delicate than those with Formvar backing and require more careful handling during specimen preparation. |
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300 Mesh |
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6. Lacey Support Films - NetMesh™ Grids: |
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 Lacey Support Film |
A lacey network support film. The holes in the lacey support film vary in size from less than a
quarter micron to more than 10 microns making them ideal for any type of specimen. Lacey support films
are extremely strong and withstand vigorous specimen preparation treatment. The specimen material is
supported by the film network but lies across or protrudes into the holes of the mesh. This allows high
definition imaging without the effects of underlying support material. Lacey films can be used for specimens
ranging from large crystals and other particulate material to virus particles. Smaller particles, such as
viruses or bacteria, tend to adhere around the inner edges of the holes, an ideal situation for high resolution
microscopy. Lacey films are also ideal for selected area electron diffraction imaging. We offer three types of lacey film: |
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 Molybdenum Trioxide Crystal on a Lacey Support Film, example of application |
 0.26µm dia. Latex on a Lacey Support Film, example of application.go to Support Film / Substrate Application Guide |
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| (a) Lacey Formvar Stabilized with Carbon: A Lacey Formvar film coated with a “heavy” layer of carbon. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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200 Mesh 300 Mesh |
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| (b) Lacey Carbon Type-A: A Lacey carbon film with a removable Formvar backing on the opposite side of the grid. When the Formvar is removed, by dipping in solvent, the Lacey carbon film remains. These films are stable under all EM operating conditions and for use where the presence of Formvar can not be tolerated. Pure Lacey Carbon is more delicate than those with Formvar backing and require more careful handling during specimen preparation. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
300 Mesh |
PELCO® Technical Notes, Removal of Formvar Film From Carbon Type-A and Silicon Monoxide Type-A Support Films, Product Numbers 01820, 21, 22, 22F, 29, 29F, 1890, 90F (PDF 81KB) |
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| (c) Lacey Silicon Monoxide on Formvar: A lacey Formvar film stabilized with Silicon Monoxide. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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300 Mesh |
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Substrate Application |
Formvar Only |
Formvar Stabilized with Carbon |
Silicon Monoxide on Formvar |
Silicon Monoxide on Type-A |
Carbon Type-A |
Carbon Type-B |
Is Lacey Film suitable for this application? |
| Applications requiring pure Formvar | B | - | - | - | - | - | No |
| Bacterial Suspensions | - | G | B | B | B | B | Yes |
| Cell fragment suspensions | - | B | B | B | B | B | Yes |
| Diffraction studies | - | - | G | G | B | B | Yes |
| EDS (energy dispersive spectrometry) | - | G | - | - | B | B | Yes |
| High resolution microscopy | - | - | G | B | B | B | Yes |
| High temperature techniques/ heating stage | - | - | - | G | B | - | Yes (Type A) |
| Low magnification microscopy | G | B | B | G | G | B | No |
| Particulate suspension, biological | - | G | B | B | B | B | Yes |
| Particulate suspension, non-biological | - | G | B | B | B | B | Yes |
| Powders, dry | - | G | B | G | G | B | No |
| Replicas & low temperature techniques | G | B | - | - | G | B | Yes (Type-A) |
| Thin sections | G | B | G | B | G | B | Yes |
| Viral suspensions | - | - | G | B | B | B | Yes |