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see also: Petri Dishes

PELCO® Glass Bottom Dishes,
Clear Wall and Black Wall

by WillCo Wells B.V., the Netherlands

Highest Quality, Sterile Glass Bottom Dishes,
for General Laboratory Use

clear wall glass-bottom petri culture dish
PELCO® Clear Wall
Glass Bottom Dishes

NEW PELCO® Clear Wall
Foil Bottom Dishes

black wall glass-bottom petri dish
PELCO® Black Wall
Glass Bottom Dishes

NEW PELCO® Black Wall
Foil Bottom Dishes


Glass Bottom Dish Assembly Kits
Build your own dishes
and save 55% or more.
Technical Information
Technical Notes
for PELCO® Glass Bottom Dishes

Positioning of Life Cells, including Ova, Sperm Cells & Embryos


Glass Bottom Dishes are used in the fields of Life Science and Environmental Research, Molecular and Cell Biology, Cell Culture and Cell Physiology, Biotechnology, Neurology, Pathology, Pharmacology and more.

These sterile tissue culture grade polystyrene glass bottom WillCo Wells Petri dishes with lid are available in two sizes, 35 x 10mm and 50 x 7mm, with clear or black plastic sidewalls. Through their advanced design features, they provide exceptional image quality needed for use on inverted microscopes and for applications such as high – and super resolution image analysis, microinjection and electro-physiology recording of fluorescent-tagged cells.

The WillCo Wells glass bottom Petri dishes use a special formulated adhesive with a low toxicity that is durable and optically clear. Glass thickness stays within 0.17mm and ra ±0.005mm and permits use of immersion objectives with water, glycerine or oil. The glass bottom is flush with the microscope stage or heating stage to ensure absolute flatness and optimum heat exchange. The optical quality of the glass bottom provides a higher effective numerical aperture (NA) producing brighter high resolution images in all types of microscopy applications.

The 50mm WillCo Wells glass bottom Petri dish features a low rim side wall. The low side wall allows easier access for micromanipulation/microinjection probes. The WillCo Wells Glass Bottom Petri dish is available in two sizes: 35mm diameter and 50mm diameter. Both sizes are available with clear or black side walls.

The 35mm dish will be compatible with most 35mm heaters and perfusion adapters.

The 50mm dishes, ‘Series 5030’ and ‘Series 5040’, have a well of respectively 30mm and 40mm, both providing a large growth area. They have a low access angle and an "easy grip" handling.

PELCO® Clear Wall Glass Bottom Dishes


glass-bottom petri culture dish
35 x 10mm Clear Wall Glass Bottom Dishes
glass bottom petri dish
Available in Blister Packs or Sleeves of 20
glass-bottom petri dish
50 x 7mm Clear Wall Glass Bottom Dishes

Applications:

  • Live Cell Microscopy
  • Multi-Photon Laser Scanning Microscopy (MPLSM)
  • High Level Laser Fluorescence
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Fluorescence Resonance Energy Transfer Microscopy (FRETM)
  • Fluorescence Redistribution After Photobleaching Microscopy (FRAPM)
  • Epifluorescence Microscopy
  • Fluorescence Imaging
  • Immunofluorescence Microscopy
  • Confocal Microscopy
  • Confocal Laser Scanning Microscopy (CLSM)
  • Phase Contrast Microscopy
  • Polarized Light Microscopy
  • Differential Interference Contrast
  • High Resolution Image Analysis
  • Time Lapsed Photography
  • Video-Enhanced Microscopy
  • Infrared imaging
  • Calcium imaging
  • Colocalization studies
  • Green Fluorescent Protein (GFP)
  • Micromanipulation
  • Microinjection
  • Super resolution imaging
  • Nanoscopy imaging

Advantages:

  • Much larger effective range, using an objective lens with a short working distance

  • No birefringence effects because of thin (Class #1.5H / 0.17mm) glass bottom

  • Low rim/side wall of the dish and a larger effective working distance make micromanipulation and microinjection possible

  • Higher effective N.A. make brighter images (in e.g.: fluorescence)

  • Higher effective N.A. make higher resolutions in image analysis
    Microscope light passes through the bottom glass in a perpendicular way, glass bottom rests directly on stage
  • Direct heating from the microscope-stage, because the glass is in direct contact (flush) with stage

  • In IVF, no more oocyte/embryo transfer is needed from one plastic dish to another, unless different media are being used, saving a lot of time (Do not put in incubator!)

  • Easy "perfusion" in the dish for continuous in and outflow of solutions, using "perfusion chamber insert", for life cells.

  • The German glass, borosilicate D263M, is used in microscopy for medical, biological and research work, e.g. in fluorescence microscopy
Fields of Application
Fertility & Infertility ICSI, spermatid identification, assisted hatching, embryo biopsy, genetics, etc.
(Cell-) Physiology Patch clamping, ion-imaging, etc.
Pathology Studies e.g. on the morphology of particles and cells, etc.
Neurology Axon growth, in and efflux studies of electrolytes, etc.
Cell Biology Cell-cell communication studies, tissue culture, etc.
Molecular Biology Studies on genetics (DNA), enzymatic activity, etc.
Pharmacology Cell research, in and outside the cell on all levels.
Plant Physiology Root hair growth, micro-parasites on roots, etc.

Product
Number
Dish
OD
Aperture
ID
Optical Glass OD
Dish
ID
Outside Height
Access Angle
14020
14028
35mm
12mm
34mm
27mm
10mm
36°
14022
14030
35mm
22mm
34mm
27mm
10mm
36°
14024
14032
50mm
30mm
49mm
44mm
7mm
17°
14026
14035
50mm
40mm
49mm
44mm
7mm
17°

 


PELCO® Clear Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod # Description Unit Price Order / Quote

Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
14020-20 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Blister Packs pkg/20 $118.95
Qty:
14020-120 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Blister Packs cs/120 647.95
Qty:
14021-20 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20 1 Sleeve 99.50
Qty:
14021-200 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20 cs/10
Sleeves
875.95
Qty:
14021-500 Glass Bottom Dish Assembly Kit, Clear, 35/12mm cs/500 1078.00
Qty:

Dish: 35mm OD x 10mm High, Glass Area: ø22mm, Access Angle: 37°
14022-20 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Blister Packs pkg/20 118.95
Qty:
14022-120 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Blister Packs cs/120 647.95
Qty:
14023-20 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20 1 Sleeve 99.50
Qty:
14023-200 35 x 10mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20 cs/10
Sleeves
875.95
Qty:
14023-500 Glass Bottom Dish Assembly Kit, Clear, 35/22mm cs/500 1078.00
Qty:

Dish: 50mm OD x 7mm High, Glass Area: ø30mm, Access Angle: 20°
14024-20 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Blister Packs pkg/20 118.95
Qty:
14024-120 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Blister Packs cs/120 647.95
Qty:
14025-20 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20 1 Sleeve 99.50
Qty:
14025-200 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20 cs/10
Sleeves
875.95
Qty:
14025-500 Glass Bottom Dish Assembly Kit, Clear, 50/30mm cs/500 1078.00
Qty:

Dish: 50mm OD x 7mm High, Glass Area: ø40mm, Access Angle: 20°
14026-20 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Blister Packs pkg/20 118.95
Qty:
14026-120 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Blister Packs cs/120 647.95
Qty:
14027-20 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20 1 Sleeve 99.50
Qty:
14027-200 50 x 7mm, PELCO® Clear Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20 cs/10
Sleeves
875.95
Qty:
14027-500 Glass Bottom Dish Assembly Kit, Clear, 50/40mm cs/500 1078.00
Qty:


PELCO® Black Wall Glass Bottom Dishes

35 x 10mm Black Wall Glass Bottom Dishes35 x 10mm Black Wall Glass Bottom Dishes 50 x 7mm Black Wall Glass Bottom Dishes50 x 7mm Black Wall Glass Bottom Dishes Applications specific to Black Wall Glass Bottom Dishes use (applications listed above for Clear Wall may also be applicable):
  • Live Cell Microscopy
  • For low background fluorescent applications
  • Confocal Microscopy
  • Inverted Microscopy
  • High Resolution Image Analysis
  • Micromanipulation
  • Differential Interference Contrast (HMC)
  • Infrared Imaging

Advantages: Are the same as above for Clear Wall Glass Bottom Dishes, PLUS

  1. The black dish, absorbs more scattering light (Considered debris!).
  2. The black dish, working in a dark room, makes one see the meniscus of the media much better.
  3. The black dish, when lid (black) is closed, protects the cells inside completely, from light.

PELCO® Black Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod # Description Unit Price Order / Quote

Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
14028-20 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Blister Packs pkg/20 $118.95
Qty:
14028-120 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Blister Packs cs/120 647.95
Qty:
14029-20 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20 1 Sleeve 99.50
Qty:
14029-200 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20 cs/10
Sleeves
875.95
Qty:
14029-500 Glass Bottom Dish Assembly Kit, Black, 35/12mm cs/500 1078.00
Qty:

Dish: 35mm OD x 10mm High, Glass Area: ø22mm, Access Angle: 37°
14030-20 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Blister Packs pkg/20 118.95
Qty:
14030-120 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Blister Packs cs/120 647.95
Qty:
14031-20 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20 1 Sleeve 99.50
Qty:
14031-200 35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20 cs/10
Sleeves
875.95
Qty:
14031-500 Glass Bottom Dish Assembly Kit, Black, 35/22mm cs/500 1078.00
Qty:

Dish: 50mm OD x 7mm High, Glass Area: ø30mm, Access Angle: 20°
14032-20 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Blister Packs pkg/20 118.95
Qty:
14032-120 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Blister Packs cs/120 670.00
Qty:
14033-20 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20 1 Sleeve 106.00
Qty:
14033-200 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20 cs/10
Sleeves
874.00
Qty:
14033-500 Glass Bottom Dish Assembly Kit, Black, 50/30mm cs/500 1078.00
Qty:

Dish: 50mm OD x 7mm High, Glass Area: ø40mm, Access Angle: 20°
14035-20 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Blister Packs pkg/20 129.50
Qty:
14035-120 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Blister Packs cs/120 647.95
Qty:
14036-20 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20 1 Sleeve 99.50
Qty:
14036-200 50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20 cs/10
Sleeves
875.95
Qty:
14036-500 Glass Bottom Dish Assembly Kit, Black, 50/40mm cs/500 1078.00
Qty:




NEW PELCO® Clear & Black Wall Foil Bottom Dishes

PEN Foil for Microdissection

50 x 70mm Clear Wall Foil Bottom Dishes50 x 7mm Clear Wall
FOIL Bottom Dishes
50 x 7mm Black Wall Foil Bottom Dishes
50 x 7mm Black Wall
FOIL Bottom Dishes
  • Application: Laser Microdissection
  • Size: 50 x 7mm
  • Bottom: PEN Foil, 2 micron
  • Aperture: 30mm
  • Vol/μL: 1100μL
  • Vents: No
  • Sterile R: 5 Years

PELCO® Clear & Black Wall Foil Bottom Dishes in individual Sterile Blister Packs or Cases of 6 Boxes of 20
Prod # Description Unit Price Order / Quote
Dish: 50mm OD x 7mm High, Foil Area: ø30mm, Access Angle: 20°
14037-20 50 x 7mm, PELCO® Clear Wall Foil Bottom Dishes (Foil 30mm), Blister Packs pkg/20 $196.00
Qty:
14037-120 50 x 7mm, PELCO® Clear Wall Foil Bottom Dishes (Foil 30mm), Blister Packs cs/120 1168.00
Qty:
14038-20 50 x 7mm, PELCO® Black Wall Foil Bottom Dishes (Foil 30mm), Blister Packs pkg/20 196.00
Qty:
14038-120 50 x 7mm, PELCO® Black Wall Foil Bottom Dishes (Foil 30mm), Blister Packs cs/120 1168.00
Qty:






Glass Bottom Dish Assembly Kit

Build your own Glass Bottom Dishes and save 55% or more compared to the pre-assembled product.

This easy do-it-yourself kit is available in two dish sizes and four bottom aperture sizes, with clear - or black walls and lids.

Each kit includes:

Special two-part Assembly Device (available separately) made of high quality anodized aluminum serves to safely, precisely, easily, and quickly position the adhesive ring and the glass coverslip bottom and evenly distribute the gluing pressure.
glass bottom dish assembly device

The assembly routine is very simple and clean, it does not involve messy glues or the need for precise aim and motor skill. A ring of matching diameter carrying the specially formulated adhesive is peeled off its backing and mounted onto the blue part of the Assembly Device. The dish bottom, guided precisely by the top protrusion of the Device, is then pressed firmly on top of the ring. This achieves transfer of the Double Sided Adhesive (DSA) ring onto the dish bottom. Now take the second silicon liner of from the ‘DSA’ ring and the now sticky dish is pressed against the corresponding glass coverslip, which has been secured within the special slot in the red part of the assembly device. After four hours at room temperature to allow the bonding to complete, the newly made glass bottom dish is ready to use. Detailed assembly instructions are included with each WillCo Wells kit.

Each kit is supplied with #1.5H thickness and ra 0.005mm coverslip glass bottoms, unless specified as #1 when placing the order (custom).

Prod # Description Unit Price Order / Quote
Clear Wall Glass Bottom Dish Assembly Kit
14021-500 Glass Bottom Dish Assembly Kit, Clear, 35/12mm cs/500 $1078.00
Qty:
14023-500 Glass Bottom Dish Assembly Kit, Clear, 35/22mm cs/500 1078.00
Qty:
14025-500 Glass Bottom Dish Assembly Kit, Clear, 50/30mm cs/500 1078.00
Qty:
14027-500 Glass Bottom Dish Assembly Kit, Clear, 50/40mm cs/500 1078.00
Qty:
Black Wall Glass Bottom Dish Assembly Kit
14029-500 Glass Bottom Dish Assembly Kit, Black, 35/12mm cs/500 1078.00
Qty:
14031-500 Glass Bottom Dish Assembly Kit, Black, 35/22mm cs/500 1078.00
Qty:
14033-500 Glass Bottom Dish Assembly Kit, Black, 50/30mm cs/500 1078.00
Qty:
14036-500 Glass Bottom Dish Assembly Kit, Black, 50/40mm cs/500 1078.00
Qty:


glass bottom dish assembly device

Assembly Device for Glass Bottom Dish Kit

The Assembly Device is a set of two high quality tools precision-machined from anodized aluminum. It serves to precisely position the adhesive ring and the glass coverslip bottom and evenly distribute the gluing pressure. A separate Assembly Device is offered for each bottom aperture size: 12mm and 22mm Devices for the 35mm dish size, larger-sized 30mm and 40mm devices for the 50mm dish size.

Prod # Description Unit Price Order / Quote
14021-0 Assembly Device for Glass Bottom Dish Kit, 12mm each $227.00
Qty:
14023-0 Assembly Device for Glass Bottom Dish Kit, 22mm each 227.00
Qty:
14025-0 Assembly Device for Glass Bottom Dish Kit, 30mm each 248.00
Qty:
14027-0 Assembly Device for Glass Bottom Dish Kit, 40mm each 248.00
Qty: