Glass Bottom Petri Dishes


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see also: Petri Dishes

PELCO® Glass Bottom Dishes,
Clear Wall and Black Wall

by WillCo Wells

Highest Quality, Sterile Glass Bottom Dishes

Clear Wall Glass Bottom Dishes - Black Wall Glass Bottom Dishes - Technical Information

Glass Bottom Dishes are used in the fields of Life Science and Environmental Research, Molecular and Cell Biology, Cell Culture and Cell Physiology, Biotechnology, Neurology, Pathology, Pharmacology and more.

These sterile tissue culture grade polystyrene glass bottom WillCo Wells Petri dishes with lid are available in two sizes, with clear or black plastic sidewalls. Through their advanced design features, they provide exceptional image quality needed for use on inverted microscopes for applications such as high resolution image analysis, microinjection and electro-physiology recording of fluorescent-tagged cells.

The glass bottom WillcCo Wells Petri dish uses a special formulated adhesive with a low toxicity that is durable and optically clear. Glass thickness stays within 0.17 ±0.01mm and permits use of immersion objectives with water, glycerin or oil. The glass bottom is flush with the microscope stage or heating stage to ensure high stability and optimum heat exchange. The optical quality of the glass bottom provides a higher effective numerical aperture producing brighter high resolution images in all types of microscopy applications.

The glass bottom WillCo Wells Petri dish features a low rim side wall. The low side wall allows easier access for micromanipulation/microinjection probes. The Glass bottom Willco Well Petri dish is available in two sizes: 35mm diameter and 50mm diameter. Both sizes are available with clear or black side walls.

The 35mm dish has outside dimensions similar to that of a Corning 35mm dish and will be compatible with most 35mm heaters and perfusion adapters.

The 50mm dish has a well of 35mm providing a large growth area. It has a low access angle and grips for easy handling.

Clear Wall Glass Bottom Dishes


glass-bottom petri culture dish
35 x 10mm Clear Wall Glass Bottom Dishes
 glass bottom petri dish
glass-bottom petri dish
50 x 7mm Clear Wall Glass Bottom Dishes
Applications:
  • Live Cell Microscopy
  • Multi-Photon Laser Scanning Microscopy (MPLSM)
  • High Level Laser Fluorescence
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Fluorescence Resonance Energy Transfer Microscopy (FRETM)
  • Fluorescence Redistribution After Photobleaching Microscopy (FRAPM)
  • Epifluorescence Microscopy
  • Fluorescence Imaging
  • Immunofluorescence Microscopy
  • Confocal Microscopy
  • Confocal Laser Scanning Microscopy (CLSM)
  • Phase Contrast Microscopy
  • Polarized Light Microscopy
  • Differential Interference Contrast
  • High Resolution Image Analysis
  • Time Lapsed Photography
  • Video-Enhanced Microscopy
  • Infrared imaging
  • Calcium imaging
  • Colocalization studies
  • Green Fluorescent Protein (GFP)
  • Micromanipulation
  • Microinjection
Advantages:
  1. Much larger effective range, using an objective lens with a short working distance

  2. No birefringence effects because of thin (0.17mm) glass bottom

  3. Low rim/side wall of the dish and a larger effective working distance make micromanipulation and microinjection possible

  4. Higher effective N.A. make brighter images (in e.g.: fluorescence)

  5. Higher effective N.A. make higher resolutions in image analysis

  6. Microscope light passes through the bottom glass in a perpendicular way, glass rests on stage

  7. Direct heating from the microscope-stage, because the glass is in direct contact (flush) with stage

  8. In IVF, no more oocyte-/embryo transfer is needed from one plastic dish to another, unless different media are being used, saving a lot of time

  9. Easy "perfusion" in the dish for continuous in and outflow of solutions, using "perfusion chamber insert"

  10. The glass is used in microscopy for medical, biological and research work, eg. fluorescence microscopy
Fields of Application:
Fertility & Sterility ICSI, spermatid identification, assisted hatching, embryo biopsy, genetics, etc.
(Cell-) Physiology Patch clamping, ion-imaging, etc.
Pathology Studies e.g. on the morphology of particles and cells, etc.
Neurology Axon growth, in and efflux studies of electrolytes, etc.
Cell Biology Cell-cell communication studies, tissue culture, etc.
Molecular Biology Studies on genetics (DNA), enzymatic activity, etc.
Pharmacology Cell research, in and outside the cell on all levels.
Plant Physiology Root hair growth, micro-parasites on roots, etc.

PELCO® Clear Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod #DescriptionUnitPriceOrder / Quote
Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
14020-20
35 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Blister Packs
pkg/20
$111.50
Qty:
14020-12035 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Blister Packscs/120709.50
Qty:
14021-2035 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Sleeve/201 Sleeve106.00
Qty:
14021-20035 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Sleeve/20cs/10
Sleeves		959.25
Qty:
Dish: 35mm OD x 10mm High, Glass Area: ø22mm, Access Angle: 37°
14022-2035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Blister Packspkg/20119.25
Qty:
14022-12035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Blister Packscs/120635.00
Qty:
14023-2035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Sleeve/201 Sleeve106.00
Qty:
14023-20035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Sleeve/20cs/10
Sleeves		862.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø30mm, Access Angle: 20°
14024-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Blister Packspkg/20119.25
Qty:
14024-12050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Blister Packscs/120635.00
Qty:
14025-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Sleeve/201 Sleeve106.00
Qty:
14025-20050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Sleeve/20cs/10
Sleeves		862.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø40mm, Access Angle: 20°
14026-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Blister Packspkg/20119.25
Qty:
14026-12050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Blister Packscs/120636.00
Qty:
14027-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Sleeve/201 Sleeve106.00
Qty:
14027-20050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Sleeve/20cs/10
Sleeves		862.00
Qty:

Black Wall Glass Bottom Dishes

glass-bottom petri dish
35 x 10mm Black Wall Glass Bottom Dishes
50 x 7mm Black Wall Glass Bottom Dishes
Applications specific to Black Wall Glass Bottom Dishes use (applications listed above for Clear Wall may also be applicable):
  • Live Cell Microscopy
  • For low background fluorescent applications
  • Confocal Microscopy
  • Inverted Microscopy
  • High Resolution Image Analysis
  • Micromanipulation
  • Differential Interference Contrast (HMC)
  • Infrared Imaging

Advantages: Same as above for Clear Wall Glass Bottom Dishes

 Cross section view of Black Wall and Clear Wall Glass Bottom Dishes:

glass-bottom petri dish

PELCO® Black Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod #DescriptionUnitPriceOrder / Quote
Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
14028-20
35 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Blister Packs
pkg/20
$111.50
Qty:
14028-12035 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Blister Packscs/120636.00
Qty:
14029-2035 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Sleeve/201 Sleeve106.00
Qty:
14029-20035 x 10mm, PELCO® Glass Bottom Dishes (Glass 12mm), Sleeve/20cs/10
Sleeves		862.00
Qty:
Dish: 35mm OD x 10mm High, Glass Area: ø22mm, Access Angle: 37°
14030-2035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Blister Packspkg/20119.25
Qty:
14030-12035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Blister Packscs/120635.00
Qty:
14031-2035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Sleeve/201 Sleeve106.00
Qty:
14031-20035 x 10mm, PELCO® Glass Bottom Dishes (Glass 22mm), Sleeve/20cs/10
Sleeves		862.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø30mm, Access Angle: 20°
140324-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Blister Packspkg/20Call
Qty:
14032-12050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Blister Packscs/120635.00
Qty:
14033-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Sleeve/201 Sleeve106.00
Qty:
14033-20050 x 7mm, PELCO® Glass Bottom Dishes (Glass 30mm), Sleeve/20cs/10
Sleeves		862.00
Qty:
Dish: 50mm OD x 7mm High, Glass Area: ø40mm, Access Angle: 20°
14035-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Blister Packspkg/20119.25
Qty:
14035-12050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Blister Packscs/120635.00
Qty:
14036-2050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Sleeve/201 Sleeve106.00
Qty:
14036-20050 x 7mm, PELCO® Glass Bottom Dishes (Glass 40mm), Sleeve/20cs/10
Sleeves		862.00
Qty:

Technical Information for Clear Wall and Black Wall PELCO® Glass Bottom Dishes
Positioning Embryos / Life Cells

Our Glass Bottom Dishes are non-cytotoxic and non-pyrogenic. However, there is always a risk of positioning embryos / life cells on or even close to the edge, where the adhesive ring is in direct contact with the media inside the dish. Therefore, it is our advice to use a position for cells, close to the center of the bottom glass (e.g. as in Figure 1).

Note: Our Glass Bottomed Dishes have passed many tests, including cytotoxicity tests. They are “non-cytotoxic” and “non-pyrogenic”.
They have passed tests with “hatched” blastocyst (mouse) embryos, showing that they are safe, when embryo’s cells are positioned in the center of the glass bottom.

Warning: Embryos, specifically “hatched” blastocyst (mouse) embryos are extremely sensitive. Therefore, we adivise all users of our Glass Bottom Dishes to be aware of the fact that they consist of:
  1. A polystyrene ring and lid (non-cytotoxic);
  2. Adhesive! (Considered cytotoxic if in close contact for more than 30 minutes);
  3. Cover glass bottom (non-cytotoxic).

Although manufacturers of “adhesives” have shown certificates confirming “non-cytotoxicity” and our Glass Bottom Dishes passed biological tests for “non-cytoxicity” — tested in a “Laboratory for Medical Device Evaluation” — it is our advice to position life cells in the center of the glass.
Position all embryos/eggs in the center of the glass bottom, as indicated in Figure 1, within a small circle or in a small square. Do not use paraffin-oil to cover the droplets!

Embryos/eggs ( / :3) should be positioned on the glass bottom as
indicated in Figure 1.

No producer/manufacturer (of e.g. Adhesives or polystyrene) guarantees the life of (hatched) embryos/cells when positioned directly on, or close to the adhesive seal (2).

Since the glass proved to be non-cytotoxic for “hatched blastocyst” mouse embryos, over a period of six (6) days, it is our advice to use the positioning, for the maximum of eight embryos, as in Figure 1.

 Explanation of Figure 1:
  1. Outer diameter of the dish: Ø50mm.
  2. Outer diameter of the glass surface, inside the dish: Ø39mm.
  3. Black bullets (8) and smallest circles (4) of the same size: embryo(s)/eggs.
  4. Center of the glass bottom, with embryos:
    a.: Within small circle Ø15mm (4 black bullets and 4 small circles).
    b.: Within square of 15mm x 15mm (black bullets)
    the center (4) is also a good location for the sperm cells
  5. Distance from adhesive (2), on the outer edge of the inner glass surface, to the embryo/egg:
= 10mm minimum.

Explanation of Figure 2:

In Figure 2, we show an example of several positions of embryos ( / :3)
or eggs that are being used for examination, of which it is our advice, never to use these positions because these cells are or may be sensitive to all existing “Medical Class VI” adhesives.

Note: It is our advice, NOT to position an embryo or an egg, inside the dish as show in Figure 2, close to ring 2.

The clear area, between 1 and 2, is the polystyrene bottom ring, to which the glass is bonded, using a “non-cytotoxic” adhesive.
  1. Outer diameter of the dish: Ø50mm.
  2. Outer diameter of the glass surface inside the dish: Ø39mm
  3. Black bullets (8): Embryo(s)/eggs.
  4. Center of the glass bottom, where the embryos should be.
  5. Distance from adhesive (2), on the outer edge of the inner glass surface, to the embryos/eggs:
= 10mm minimum.


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Page Last Updated: October 7, 2009