Glass Bottom Dishes are used in the fields of Life Science and Environmental Research, Molecular and Cell Biology,
Cell Culture and Cell Physiology, Biotechnology, Neurology, Pathology, Pharmacology and more.
These sterile tissue culture grade polystyrene glass bottom WillCo Wells Petri dishes with lid are available in two
sizes, with clear or black plastic sidewalls. Through their advanced design features, they provide exceptional image
quality needed for use on inverted microscopes for applications such as high resolution image analysis, microinjection
and electro-physiology recording of fluorescent-tagged cells.
The glass bottom WillcCo Wells Petri dish uses a special formulated adhesive with a low toxicity that is durable and optically
clear. Glass thickness stays within 0.17 ±0.01mm and permits use of immersion objectives with water, glycerin or oil.
The glass bottom is flush with the microscope stage or heating stage to ensure high stability and optimum heat exchange. The
optical quality of the glass bottom provides a higher effective numerical aperture producing brighter high resolution images
in all types of microscopy applications.
The glass bottom WillCo Wells Petri dish features a low rim side wall. The low side wall allows easier access for
micromanipulation/microinjection probes. The Glass bottom Willco Well Petri dish is available in two sizes: 35mm diameter and
50mm diameter. Both sizes are available with clear or black side walls.
The 35mm dish has outside dimensions similar to that of a Corning 35mm dish and will be compatible with most 35mm heaters and
perfusion adapters.
The 50mm dish has a well of 35mm providing a large growth area. It has a low access angle and grips
for easy handling.
Clear Wall Glass Bottom Dishes
35 x 10mm Clear Wall Glass Bottom Dishes
50 x 7mm Clear Wall Glass Bottom Dishes
Applications:
Live Cell Microscopy
Multi-Photon Laser Scanning Microscopy (MPLSM)
High Level Laser Fluorescence
Fluorescence Lifetime Imaging Microscopy (FLIM)
Fluorescence Resonance Energy Transfer Microscopy (FRETM)
Fluorescence Redistribution After Photobleaching Microscopy (FRAPM)
Epifluorescence Microscopy
Fluorescence Imaging
Immunofluorescence Microscopy
Confocal Microscopy
Confocal Laser Scanning Microscopy (CLSM)
Phase Contrast Microscopy
Polarized Light Microscopy
Differential Interference Contrast
High Resolution Image Analysis
Time Lapsed Photography
Video-Enhanced Microscopy
Infrared imaging
Calcium imaging
Colocalization studies
Green Fluorescent Protein (GFP)
Micromanipulation
Microinjection
Advantages:
Much larger effective range, using an objective lens with a short working distance
No birefringence effects because of thin (0.17mm) glass bottom
Low rim/side wall of the dish and a larger effective working distance make micromanipulation and microinjection possible
Higher effective N.A. make brighter images (in e.g.: fluorescence)
Higher effective N.A. make higher resolutions in image analysis
Microscope light passes through the bottom glass in a perpendicular way, glass rests on stage
Direct heating from the microscope-stage, because the glass is in direct contact (flush) with stage
In IVF, no more oocyte-/embryo transfer is needed from one plastic dish to another, unless different
media are being used, saving a lot of time
Easy "perfusion" in the dish for continuous in and outflow of solutions, using "perfusion chamber insert"
The glass is used in microscopy for medical, biological and research work, eg. fluorescence microscopy
Fields of Application:
Fertility & Sterility
ICSI, spermatid identification, assisted hatching, embryo biopsy, genetics, etc.
Cross section view of Black Wall and Clear Wall Glass Bottom Dishes:
(Cell-) Physiology
Patch clamping, ion-imaging, etc.
Pathology
Studies e.g. on the morphology of particles and cells, etc.
Neurology
Axon growth, in and efflux studies of electrolytes, etc.
Cell Biology
Cell-cell communication studies, tissue culture, etc.
Molecular Biology
Studies on genetics (DNA), enzymatic activity, etc.
Pharmacology
Cell research, in and outside the cell on all levels.
Plant Physiology
Root hair growth, micro-parasites on roots, etc.
PELCO® Clear Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod #
Description
Unit
Price
Order / Quote
Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
Applications specific to Black Wall Glass Bottom Dishes use (applications listed above for Clear Wall may also be applicable):
Live Cell Microscopy
For low background fluorescent applications
Confocal Microscopy
Inverted Microscopy
High Resolution Image Analysis
Micromanipulation
Differential Interference Contrast (HMC)
Infrared Imaging
Advantages: Same as above for Clear Wall Glass Bottom Dishes
PELCO® Black Wall Glass Bottom Dishes in individual Sterile Blister Packs or Bulk Sterile Sleeves of 20
Prod #
Description
Unit
Price
Order / Quote
Dish: 35mm OD x 10mm High, Glass Area: ø12mm, Access Angle: 37°
14028-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Blister Packs
pkg/20
$129.50
14028-120
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Blister Packs
cs/120
670.00
14029-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20
1 Sleeve
106.00
14029-200
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 12mm), Sleeve/20
cs/10 Sleeves
874.00
Dish: 35mm OD x 10mm High, Glass Area: ø22mm, Access Angle: 37°
14030-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Blister Packs
pkg/20
129.50
14030-120
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Blister Packs
cs/120
670.00
14031-20
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20
1 Sleeve
106.00
14031-200
35 x 10mm, PELCO® Black Wall Glass Bottom Dishes (Glass 22mm), Sleeve/20
cs/10 Sleeves
874.00
Dish: 50mm OD x 7mm High, Glass Area: ø30mm, Access Angle: 20°
14032-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Blister Packs
pkg/20
129.50
14032-120
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Blister Packs
cs/120
670.00
14033-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20
1 Sleeve
106.00
14033-200
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 30mm), Sleeve/20
cs/10 Sleeves
874.00
Dish: 50mm OD x 7mm High, Glass Area: ø40mm, Access Angle: 20°
14035-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Blister Packs
pkg/20
129.50
14035-120
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Blister Packs
cs/120
670.00
14036-20
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20
1 Sleeve
106.00
14036-200
50 x 7mm, PELCO® Black Wall Glass Bottom Dishes (Glass 40mm), Sleeve/20
cs/10 Sleeves
874.00
Technical Information for Clear Wall and Black Wall PELCO®
Glass Bottom Dishes
Positioning Embryos / Life Cells
Our Glass Bottom Dishes are non-cytotoxic and non-pyrogenic. However, there is always
a risk of positioning embryos / life cells on or even close to the edge, where the
adhesive ring is in direct contact with the media inside the dish. Therefore, it
is our advice to use a position for cells, close to the center of the bottom glass
(e.g. as in Figure 1).
Note: Our Glass Bottomed Dishes have passed many tests, including
cytotoxicity tests. They are “non-cytotoxic” and “non-pyrogenic”.
They have passed tests with “hatched” blastocyst (mouse) embryos, showing
that they are safe, when embryo’s cells are positioned in the center of the
glass bottom.
Warning: Embryos, specifically “hatched” blastocyst
(mouse) embryos are extremely sensitive. Therefore, we adivise all users of our
Glass Bottom Dishes to be aware of the fact that they consist of:
A polystyrene ring and lid (non-cytotoxic);
Adhesive! (Considered cytotoxic if in close contact for more than 30 minutes);
Cover glass bottom (non-cytotoxic).
Although manufacturers of “adhesives” have shown certificates confirming
“non-cytotoxicity” and our Glass Bottom Dishes passed biological tests
for “non-cytoxicity” — tested in a “Laboratory for Medical
Device Evaluation” — it is our advice to position life cells in the center
of the glass.
Position all embryos/eggs in the center of the glass bottom, as indicated in Figure
1, within a small circle or in a small square. Do not use paraffin-oil to cover
the droplets!
Explanation of Figure 1:
Embryos/eggs (
/
:3) should be positioned on the glass bottom as indicated in Figure 1.
No producer/manufacturer (of e.g. Adhesives or polystyrene) guarantees the life
of (hatched) embryos/cells when positioned directly on, or close to the adhesive
seal (2).
Since the glass proved to be non-cytotoxic for “hatched blastocyst”
mouse embryos, over a period of six (6) days, it is our advice to use the positioning,
for the maximum of eight embryos, as in Figure 1.
Outer diameter of the dish: Ø50mm.
Outer diameter of the glass surface, inside the dish: Ø39mm.
Black bullets (8) and smallest circles (4) of the same size: embryo(s)/eggs.
Center of the glass bottom, with embryos:
Within small circle Ø15mm (4 black bullets and 4 small circles).
Within square of 15mm x 15mm (black bullets)
the center (4) is also a good location for the sperm cells
Distance from adhesive (2), on the outer edge of the inner glass surface, to the embryo/egg: = 10mm minimum.
Explanation of Figure 2:
In Figure 2, we show an example of several positions of embryos (
/
:3) or eggs that are
being used for examination, of which it is our advice, never to use these positions
because these cells are or may be sensitive to all existing “Medical Class
VI” adhesives.
Note: It is our advice, NOT to position an embryo or an egg, inside
the dish as show in Figure 2, close to ring 2.
The clear area, between 1 and 2, is the polystyrene bottom ring, to which the glass
is bonded, using a “non-cytotoxic” adhesive.
Outer diameter of the dish: Ø50mm.
Outer diameter of the glass surface inside the dish: Ø39mm
Black bullets (8): Embryo(s)/eggs.
Center of the glass bottom, where the embryos should be.
Distance from adhesive (2), on the outer edge of the inner glass surface, to the embryos/eggs: = 10mm minimum.
35 x 10mm Black Wall Glass Bottom Dishes
50 x 7mm Black Wall Glass Bottom Dishes
/
:3) should be positioned on the glass bottom as indicated in Figure 1.
= 10mm minimum.
