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Histology, page 1
Biological Microscope Overview
Biological Microscope Comparison

BA400 Biological Light Microscope

Clinical and research grade microscope


Motic BA200 Biological Light Microscope
 

The BA400 with CCIS optics allow new accessories and functions to be incorporated in the BA400 series microscope. The CCIS (Color Corrected Infinity System) optics delivers uncompromised optical clarity and unrivalled versatility. The CCIS infinity design has succeeded in achieving longer working distance objectives with higher numerical aperture giving increased optical performance and versatility. The BA400 microscope stand, with its innovative mechanical design, represents an excellent platform for high quality optical imaging and ease of use for longer periods of time. The microscope incorporates many features found on more expensive microscopes.

A complete range of eyepieces and objectives is available to configure the BA400 for your application: all options are shown in the BA400 Biological Microscope System Diagram. The fluorescence imaging attachment transforms the BA400 into a high quality fluorescence microscope with an outstanding price / performance ratio.

Motic BA300 microscope stand

Microscope Stand

The BA400 has been designed for optimum performance coupled to ergonomic use. The microscope stand integrates all functions providing effective ergonomics and maximum expandability. The wide arm provides strength and rigidity, the inverted "Y" design gives enhanced lateral stability. The BA400 is designed to meet the requirements of a personal microscope and has a minimized footprint to conserve the limited desk space in today's modern laboratories.

Light Source

The 6V/30W halogen Koehler illumination system provides bright, even illumination at any magnification. The centerable lamp is housed externally and has an externally operated control mechanism for all facets of illumination. A diffuser is located in the base of the microscope and can be easily operated through a selector switch.

Observation Tubes

In order to maintain absolute parfocality, the Siedentopf eyepiece tubes will not change their length when interpupillary adjustments are made. The inclination of 30° for the binocular and trinocular tubes is chosen for comfort and posture management. By using the optionally available eye level risers, the user can raise the eye level up to a maximum of 60mm in 20mm steps. Two trinocular versions are also available featuring professional light distribution sliders either for 100% binocular /100% photo tube or 100% binocular and 20/80% for binocular/photo. The trinocular heads are ideal for digital documentation and video projection.

Focusable Eyepieces

The standard Widefield 10x eyepieces of the BA400 with an enlarged field of view of 22mm provides enlarged viewing fields for faster scanning and easier viewing. Parfocality of focus is provided by independent diopter adjustment on each eyepiece. Users wearing eyeglasses will find the eyepieces' high eye point design very comfortable to use. Various reticles for measuring and counting are available for these eyepieces.

Quintuple Revolving Nosepiece

The reversed design of the nosepiece means that when using the BA400, the user has more space to change specimen slides offering easy access and limiting damages to slides or objectives. The nosepiece features long-life a ball bearing mechanism with positive click-stop to ensure that each change of objective is accurate and centered.

Condenser

The newly developed swing-out Achromat condenser with a numerical aperture of 0.9 provides homogeneous illumination for observation and microphotography at all magnification from 2x to 100x. The substage condenser is height adjustable via a precise rack and pinion mechanism and has a dovetail mount with clamp screw.

The phase contrast turret condenser with a numerical aperture of 1.25 has four phase positions and a BF position with iris diaphragm. This type of condenser is particularly useful for studies where rapid change between illumination techniques is required.


Motic BA300 microscope stand

Rectangular Mechanical Stage

The BA400 comprises a large (174mm x145mm) hard coated mechanical stage with a travel range of 76mm x 50mm. Controlling the movement of the stage is made easy by low positioned coaxial control knobs. The stage surface is protected with a hard coating against abrasion and wear. The user can set the upper stage drive stop to prevent damage to objectives or to accommodate slide of different thickness. The stage can be rotated for composition in photomicrography.

Polarized light

Two filter slots in the upper part of the stand accommodate slides with an analyzer and a first order red compensator (535nm) for screening possible cases of gout. The slide mounted analyzer is removable for quick changes between polarizing and standard bright field imaging. The polarizer is installed over the field lens.

BA400 Technical Specifications at a glance
Optical System Parfocal distance 45mm CCIS Optical System (Color Corrected Infinity System)
Observation tube

Siedentopf 30° Binocular. Optional Trinocular heads.

Eyepiece  Widefield high eyepoint WF 10x (FN22) with diopter adjustment
Nosepiece Quintuple reversed
Coarse/Fine focusing  Coaxially positioned control knobs, coarse 42mm/rotation, fine 0.2mm/rotation. Min. increment 2µm. Coarse motion torque adjustable.
Stage Rectangular 174 x 145mm surface area, 76 x 50mm cross travel. Hard coated surface, vertical movement range is 27mm.
Substage Illumination 6V/30w Quartz Halogen lamp in centerable housing and integrated diffuser
Filter Blue

 

Prod #DescriptionUnitPriceOrder / Quote
Complete Systems for the BA400
22432-10
BA400 Binocular Microscope, WF10x/22 Eyepieces, Plan Achromat Objectives 4x, 10x, 40x S, 100x S-Oil, Achromat swing-out condenser NA 0.9, 6V/30W Quartz illumination
each
$2,626.00
Qty:
22432-12
BA400 Trinocular Microscope, WF10x/22 Eyepieces, Plan Achromat Objectives 4x, 10x, 40x S, 100x S-Oil, Achromat swing-out condenser NA 0.9, 6V/30W Quartz illumination
each
2,783.00
Qty:
Main Body
22432-15
BA400 Main Body with reversed quintuple nosepiece, mechanical stage, Koehler illumination 6V/30W
each
1,105.00
Qty:
all BA400 microscopes include 45mm blue filter, 5ml immersion oil, power cord, Allen hex key, vinyl dust cover. Universal power supply 100-240V

phase centering, phase contrast condenser

Prod #DescriptionUnitPriceOrder / Quote
Accessories and Options for the BA400
Observation Tube
22432-151
Siedentopf Binocular Head, 30° inclined
each
$158.00
Qty:
22432-152
Siedentopf Trinocular Head, 30° inclined (100% bin, 20/80% bin/video)
each
315.00
Qty:
22432-153
Siedentopf Trinocular Head, 30° inclined (100% bin, 0/100% bin/video)
each
315.00
Qty:
Eyepieces (each)
22432-312
WF PL 10x (FN20), focusable with diopter adjustment
each
79.00
Qty:
22432-313
WF PL 10x (FN20), with cross line, focusable with diopter adjustment
each
115.00
Qty:
22431-314
WF PL 12.5x (FN16), focusable with diopter adjustment
each
145.00
Qty:
22431-315
WF PL 15x (FN14.5), focusable with diopter adjustment
each
95.00
Qty:
Reticles (each)
22431-301
Cross Line Reticle, ø23mm
each
41.00
Qty:
22431-302
Cross Line Reticle with micrometer (100 div in 10mm), ø23mm
each
41.00
Qty:
22431-303
Micrometer Reticle (100 div in 10mm), ø23mm
each
41.00
Qty:
Plan Achromat Objectives - PL
22431-350
CCIS Plan Achromat Objective 4x/0.1 (WD 7mm)
each
84.00
Qty:
22431-351
CCIS Plan Achromat Objective 10x/0.25 (WD 4.3mm)
each
126.00
Qty:
22431-352
CCIS Plan Achromat Objective 20x/0.4 (WD 1.3mm)
each
158.00
Qty:
22431-353
CCIS Plan Achromat Objective 40x/0.65/S (WD 0.4mm)
each
252.00
Qty:
22431-354
CCIS Plan Achromat Objective 100x/1.25/S-Oil (WD 0.13mm)
each
368.00
Qty:
Plan Fluorite Objectives - PL FLR
22432-341
CCIS Plan Fluar, 4x
each
326.00
Qty:
22432-342
CCIS Plan Fluar, 10x
each
552.00
Qty:
22432-343
CCIS Plan Fluar, 20x
each
620.00
Qty:
22432-344
CCIS Plan Fluar, 40x
each
725.00
Qty:
22432-340
CCIS Plan Fluar, 50x (oil)
each
835.00
Qty:
22432-345
CCIS Plan Fluar, 100x (oil)
each
1,570.00
Qty:
Plan Phase Objectives - PL PH
22431-345
CCIS Plan Phase Objective 10x/0.25
each
137.00
Qty:
22431-346
CCIS Plan Phase Objective 20x/0.4
each
169.00
Qty:
22431-347
CCIS Plan Phase Objective 40x/0.65S
each
263.00
Qty:
22431-348
CCIS Plan Phase Objective 100x/1.25/S-oil
each
379.00
Qty:
Phase Contrast Equipment / Condensers
22432-372
Achromat Swing-out Condenser, N.A. 090/013
each
210.00
Qty:
22432-374
LWD Achromat Condenser, N.A. 0.65/W.D. 10.8
each
315.00
Qty:
22431-365
Phase contrast 5 position turret condenser for 10x, 20x, 40x, 100x, BF (use with Plan Phase objectives)
each
315.00
Qty:
22431-366
Phase contrast 5 position turret condenser for BF, DF, 10x, 40x, 100x (use with Plan Phase objectives)
each
315.00
Qty:
22431-370
Phase Centering Telescope
each
121.00
Qty:
Polarizing Equipment
22432-412
Analyzer Slider with Rotating Mount (360° Rotary)
each
105.00
Qty:
22432-413
Polarizer (simple plug in type)
each
48.00
Qty:
22432-414
Polarizer (simple add on type)
each
32.00
Qty:
22432-415
First Order Red Compensator (535nm)
each
310.00
Qty:
Stages
22432-440
Mechanical Stage with left-hand control (2 slides)
each
165.00
Qty:
22432-442
Mechanical Stage with right-hand control (2 slides)
each
165.00
Qty:
Photo and C-mount Video
22430-400
2.5x SLR projection lens, requires photo eyepiece
each
105.00
Qty:
22430-402
Photo Eyepiece 2.5x SLR Projection Lens, requires photo eyepiece
each
179.00
Qty:
22430-404
Photo Eyepiece 4x for photo adapter
each
142.00
Qty:
22430-405
1.0x C-mount Camera Adapter (no lens)
each
132.00
Qty:
22430-406
0.65x C-mount Adapter for 2/3" Chip Sensors
each
132.00
Qty:
22430-407
0.5x C-mount Camera Adapter for 1/2" Chip Sensors
each
132.00
Qty:
Optional Accessories and Replacements
22432-320
Eye Level Risers (20mm)
each
58.00
Qty:
22430-320
Yellow Filter (45mm)
each
25.00
Qty:
22430-421
Green Filter (45mm)
each
16.00
Qty:
22431-422
Blue Filter (45mm)
each
10.00
Qty:
22431-423
Didymium Filter (45mm)
each
60.00
Qty:
22432-424
Neutral Density Filter (45mm)
each
16.00
Qty:
22432-425
Green Interference Filter (45mm)
each
29.00
Qty:
22432-235
Bulb Centering Filter (45mm)
each
11.00
Qty:
22431-230
Quartz Halogen Lamp, 6V/30W
each
20.00
Qty:
22430-240
Immersion Oil, 5ml
each
8.00
Qty:
EF EPI Fluorescence Attachement
22432-500
Complete EPI-Fluorescence Attachement Kit, consists of the following individually listed parts
each
P.O.R.
Qty:
22432-510
EP-Fluorescence Attachement with Filter Cassette and Mercury Lamp
each
1,759.00
Qty:
22434-511
Lamphouse HBO 100W
each
294.00
Qty:
22434-512
Collector Lens
each
156.00
Qty:
22434-513
Mercury Lamp Socket for HBO 100W
each
575.00
Qty:
22432-515
Lamp Centering Tool
each
98.00
Qty:
22434-516
Mercury Lamp HG-100W
each
510.00
Qty:
22434-517
UL/CSA Power Cord
each
12.00
Qty:
Standard Fluorescence Filter Sets
22434-520
DAPI and Hoechst set:
Exciter D350/50x
Dichroic 400DCLP
Emitter D460/50m
each
868.00
Qty:
22434-521
FITC/RSGFP/Fluo 3/Dio Acradine Orange (DNA) set:
Exciter D480/30x
Dichroic 505DCLP
Emitter D535/40m
each
868.00
Qty:
22434-522
TRITC (Rhodamine)/DiI/Cy3 set:
Exciter D540/25x
Dichroic 565DCLP
Emitter D605/55m
each
868.00
Qty:
22434-523
Texas Red/Cy3.5 set:
Exciter D560/40x
Dichroic 595DCLP
Emitter D630/60m
each
868.00
Qty:
22434-524
Cy5, Alexa Fluor 633, Alexa Fluor 647 set:
Exciter HQ620/60x
Dichroic Q660LP
Emitter HQ700/75m
each
865.00
Qty:
22434-525
Cyan GFP set:
Exciter D436/20x
Dichroic 455DCLP
Emitter D480/40m
each
868.00
Qty:
22434-526
Endow GFP Bandpass Emission set:
Exciter HQ470/40x
Dichroic Q495LP
Emitter HQ525/50m
each
1,035.00
Qty:
22434-527
Yellow GFP BP (10C/Topaz) set:
Exciter HQ500/20x
Dichroic Q515LP
Emitter HQ535/30m
each
885.00
Qty:
22434-550
Empty Filter Set
each
158.00
Qty:

Digital Cameras for BA400 Trinocular Microscope, Prod. No. 22432-12

Requires CCD Adapter, Prod. No. 22430-407 (see above).

Prod #DescriptionUnitPriceOrder / Quote
Moticam Digital Cameras
2290-81
Moticam 1000, 1.3 Megapixel CCD Camera
each
$399.00
Qty:
2290-100
Moticam 2000, 2.0 Megapixel Digital Color Camera
each
699.00
Qty:
2290-120
Moticam 2300, 3.0 Megapixel Digital Color Camera
each
899.00
Qty:
2290-125
Moticam 2500, 5.0 Megapixel Digital Color Camera
each
1,295.00
Qty:


BA400 Dimensions (unit: mm)

go to: Moticam Digital Cameras
go to: Motic MLC-150C Fiber Optic Illumination System