Histology Overview
Biological Microscope Overview
Biological Microscope Comparison
Light Microscope Calibration Standards

Motic® AE31 Elite
Research Grade Inverted Microscope

With High Quality Optics and Superior Mechanical Design

motic dmba200 biological light microscope
AE31 Elite Binocular
AE31 Elite Trinocular

The Motic® AE31 Elite inverted microscopes provide excellent optical quality and unmatched operational convenience. The Color Corrected Infinity Optical System (CCIS®) allows new accessories and functions to be incorporated into the AE30/31 inverted microscopes at any time. The CCIS® infinity design has succeeded in achieving longer working distances with higher Numerical Apertures (NA) representing a significant improvement in optical performance and versatility.

A complete range of eyepieces and objectives are available to configure the AE31 Elite for your application.

Microscope Stand

The design of the AE31 Elite series inverted microscopes has been optimized to integrate all functions enabling effective ergonomics and maximum expandability. The wide base provides strength and rigidity; furthermore the inverted "Y" support at the back on the base provides extra lateral stability. The size of the microscope is compact to minimize the footprint and conserve the limited desk space available in modern laboratories.

The ergonomic design of the AE31 Elite enables easy and stress-free operation. The coaxial coarse/fine focusing knobs, controls for the stage and light intensity, are placed conveniently at your finger tips to minimize fatigue.

Light Source

The 6V/30W halogen Koehler illumination system provides bright, even illumination at any magnification. The "only-one-in-its-class" centerable lamp is housed externally and has an externally operated control mechanism for all facets of illumination. A segmented illumination intensity indicator is ideally located for easy viewing.

An energy saving Auto-Off feature turns the instrument off if not used for 15 minutes. New Light Memory function, based on encoded five position nosepiece, will recall the last light intensity setup when changing ot a respective objective. Once set, there is no need to adjust the illumination when changing magnification.

Observation Tubes

In order to maintain absolute parfocality, the Siedentopf eyepiece tubes will not change their length when interpupillary adjustments are made. The inclination of 45° for the binocular and trinocular tubes is chosen for comfort and posture management.

Trinocular version features professional light distribution slider for 100% binocular and 20/80% for binocular/photo. The trinocular heads are ideal for digital documentation and video projection.

Focusable Eyepieces

The standard Wide field 10x eyepieces for the AE31 Elite with an enlarged field of view of 22mm provides enlarged viewing fields for faster scanning and easier viewing. Parfocal focus is provided by independent diopter adjustment on each eyepiece. Users wearing eyeglasses will find the eyepieces' high eye point design very comfortable to use. Various graticules for measuring and counting are available for these eyepieces.

Quintuple Revolving Nosepiece

The revolving left side facing nosepiece accepts five objectives. It runs on precision ball bearings and has internal positive click stops so that the image remains centered after each change in magnification. The internal components and the optics are sealed against fluid spills to ensure reliable and trouble-free usage. This allows the operator to concentrate fully on imaging the specimen and not to worry about accidentally damaging the microscope.


The CCIS® objectives for inverted microscopes have long free working distances in comparison to normal objectives of the same magnification. The objectives are optically corrected to compensate for 1.1mm thick glass slides and provide easy routine operation. RMS thread.

Phase Contrast Microscopy

Phase contrast is the most popular optical contrast method for viewing the detailed structure of unstained or living specimens. The Phase Plan Achromat objectives, coupled with the easy-to-operate phase slider, provide outstanding contrast for the most demanding applications. For a quick and easy change of magnification, Ph1 annular ring is provided on the centerable phase slider.

Condenser Mount

The centerable condenser mount is height adjustable with rack and pinion and is dovetail mounted on the illuminating pillar with a solid clamp screw. The ELWD condenser with a numerical aperture of 0.30 and a working distance of 72mm is suitable for objectives of magnification from 4x - 40x with an aperture diaphragm in the bright field Koehler illumination and for phase contrast.

The phase annular ring Ph1 for 10x, 20x and 40x and Ph0 for 4x are centered by Allen Hex keys on the centerable slider.

Mechanical Stage and accessories

The standard stage is a fixed stage plate: the stage can be widened on both sides with auxiliary stage plates. A hard coating protects the stage surface from abrasion and wear. The tempered glass stage insert allows for checking the objective being used without removing the specimen from the stage.

An optional attachable mechanical stage with Well plate holder with low positioned coaxial controls is available. The mechanical stage also accepts interchangeable specimen holders for Petri dishes and glass slides.

Digital Documentation

The integration of the AE31 Elite Trinocular microscope with the Moticam®series of digital cameras delivers crisp video images. All Moticam® cameras come with software to transform the AE31 Elite into an analysis and documentation station. With USB 2.0 output to the computer, the system provides high resolution imaging in both real-time and capture modes. Should you choose a Motic® or third-party camera, Motic®provides a range of C-mount adapters which are necessary for trinocular port attachment.

Standard Photomicrography

Use of a traditional SLR style camera requires a trinocular version of the AE31 Elite. A mechanical T-mount adapter combined with a photo eyepiece (2.5x or 4x) must be fitted to the microscope.

A T/T2 adapter (supplied by the camera manufacturer) may also be necessary for this application.

Motic® AE31 Elite Technical Specifications
Optical System Parfocal distance 45mm CCIS® Optical System (Color Corrected Infinity System)
Observation tube
Siedentopf 45° Binocular or Trinocular heads
Eyepiece  Widefield WF 10x (FN22) with diopter adjustment
Nosepiece Quintuple reversed with splash protection
Coarse/Fine focusing  Coaxially positioned control knobs, coarse 42mm/rotation, fine 0.2mm/rotation. Min. increment 2µm. Coarse motion torque adjustable.
Stage Plain stage 200 x 260mm, hard coated surface.
Substage Illumination 6V/30W Quartz Halogen lamp in centerable housing and integrated diffuser
Condenser ELWD NA 0.30/72mm or LWD NA 0.50/28mm both focusable
Power Requirements Universal; 110-240V; 50/60Hz

Prod # Description Unit Price Order / Quote
Complete Systems for the Motic® AE31 Elite
22439-10 AE31 Elite Binocular Inverted Microscope, WF10x/22 Eyepieces, Plan Achromat PL4x Plan Achromat Phase 10x and 20x objectives, ELWD condenser, Phase slider, PH1 phase centering telescope, plain stage, 6V/30W Quartz illumination each P.O.R.
22439-12 AE31 Elite Trinocular (80/20) Inverted Microscope, WF10x/22 Eyepieces, Plan Achromat PL4x Plan Achromat Phase 10x and 20x objectives, ELWD condenser, Phase slider, PH1, and phase centering telescope, plain stage, 6V/30W Quartz illumination each P.O.R.
Main Body
22439-15 AE31 Elite Main Body with quintuple nosepiece, plain stage, 45° binocular, Koehler illumination 6V/30W each 1921.00
22439-16 AE31 Elite (80/20) Main Body with quintuple nosepiece, plain stage, 45° trinocular, Koehler illumination 6V/30W each 2349.00
All AE31 Elite microscopes include 45mm blue, neutral density and green filter, power cord, allen hex key, vinyl dust cover. Universal power supply 110-240V, 50/60Hz

Prod # Description Unit Price Order / Quote
Accessories and Options for the Motic® AE31 Elite
Eyepieces (each)
22439-312 WF PL 10x (FN22), focusable with diopter adjustment each $81.00
22439-314 WF PL 12.5x (FN16), focusable with diopter adjustment each 155.00
22439-315 WF PL 15x (FN14.5), focusable with diopter adjustment each 107.00
Reticles (each)
22455-301 Cross Line Reticle, ø23mm each 44.00
22439-302 Cross Line Reticle with Micrometer Scale(100 div in 10mm), ø23mm each 44.00
22455-303 Micrometer Scale Reticle (100 div in 10mm), ø23mm each 44.00
Plan Achromat Objectives - PL INV
22439-350 CCIS® Plan Achromatic Objective 4x, NA 0.1 / WD 12.6 each 97.00
22439-351 CCIS® Plan Achromatic Objective 10x, NA 0.25 / WD 4.1 each 140.00
Plan Achromat Long Working Distance Objectives - PL LWD INV
22439-352 CCIS® Plan Achromatic Objective 20x, NA 0.3 / WD 4.7 / each 214.00
22439-353 CCIS® Plan Achromatic Objective 40x, NA 0.5 / WD 3.0 each 290.00
Plan Achromat Phase Objectives - PL PH LWD INV
22439-345 CCIS® Plan Achromat Phase Objective 4x, NA 0.10 / WD 12.6 each 140.00
22439-346 CCIS® Plan Achromat Phase Objective 10x, NA 0.25 / WD 4.1 each 183.00
Plan Achromat Phase Long Working Distance Objectives
22439-347 CCIS® Plan Achromat Phase Objective 20x, NA 0.3 / WD 4.7 each 257.00
22439-348 CCIS® Plan Achromat Phase Objective 40x, NA 0.5 / WD 3.0 each 332.00
22439-372 ELWD NA 0.30, WD 72mm Condenser each 326.00
22439-373 LWD NA 0.50, WD 28mm Condenser each 568.00
Phase Contrast Equipment
22439-365 Phase Slider, centerable BF each 143.00
22439-366 Phase Stop, 0.3 PH0 (for ELWD) each 37.00
22439-367 Phase Stop, 0.3 PH1 (for ELWD) each 37.00
22439-368 Phase Stop, 0.5 PH0 (for LWD) each 37.00
22439-369 Phase Stop, 0.5 PH1 (for LWD) each 37.00
22431-370 Phase Centering Telescope each 90.00
Stage Accessories
22439-440 Glass Insert each 18.00
22439-441 Metal Insert each 37.00
22439-442 Auxiliary Stages (paired) each 32.00
22443-443 Universal attachable mechanical stage with well plate holder (128 x 86mm) each 665.00
22434-444 Well Plate Holder (128 x 88mm) each 84.00
22434-445 35mm Petri Dish Holder each 59.00
22434-446 54mm Petri Dish Holder each 59.00
22434-447 65mm Petri Dish Holder each 59.00
22434-448 Hemacytometer Holder each 59.00
Photo and C-mount Video Adapters - Necessary for Camera Mounting on Trinocular Port
22439-400 2.5x SLR Projection Lens, requires photo eyepiece each 112.00
22434-402 Photo Eyepiece 2.5x for photo adapter each 199.00
22434-404 Photo Eyepiece 4x for photo adapter each 153.00
22439-405 1.0x C-mount Camera Adapter for 2/3" chip sensors each 142.00
22439-406 0.65x C-mount Adapter for 2/3" chip sensors each 142.00
22439-407 0.5x C-mount Camera Adapter for 1/2" chip sensors each 142.00
22439-408 0.35x C-mount Camera Adapter for 1/3" chip sensors each 195.00
Optional Accessories and Replacements
22431-422 Blue Filter (45mm) each 12.00
22431-423 Didymium Filter (45mm) each 69.00
22434-424 Neutral Density Filter ND-2 (45mm) each 18.00
22432-425 Green Interference Filter (45mm) each 33.00
22431-230 Quartz Halogen Lamp, 6V/30W each 17.00
LED Illumination Options
22439-500 4500K LED Bulb Module each 86.00
22439-501 6500K LED Bulb Module each 86.00

Motic Microscope Cameras

Moticam® Digital Color Cameras
for AE31 Elite Inverted Microscope

go to:
Background information on CCD and CMOS technology
go to: Motic® MLC-150C Fiber Optic Illumination System
go to: Standard Series Stage Micrometers for Transmitted and Reflected Light
go to: Pro Series Stage Micrometers for Transmitted and Reflected Light