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Histology, page 1
Biological Microscope Overview
Biological Microscope Comparison

Motic AE30 and AE31

Research Grade Inverted Microscopes

With high quality optics / superior mechanical design
Motic DMBA200 Biological Light Microscope

The Motic AE30 and Motic AE31 inverted microscopes provide excellent optical quality and unmatched operational convenience. The Color Corrected Infinity Optical System (CCIS) allows new accessories and functions to be incorporated into the AE30/31 inverted microscope at any time. The CCIS infinity design has succeeded in achieving longer working distances with higher Numerical Apertures (NA) representing a significant improvement in optical performance and versatility.

A complete range of eyepieces and objectives are available to configure the AE30/31 for your application: all options are shown in the AE30/31 Inverted Microscope Systems Diagram.

Microscope Stand

The design of the AE 30/32 series inverted microscopes has been optimized to integrate all functions enabling effective ergonomics and maximum expandability. The wide base provides strength and rigidity; furthermore the inverted "Y" support at the back on the base provides extra lateral stability. The size of the microscope is compact to minimize the footprint and conserve the limited desk space available in modern laboratories.

The ergonomic design of the AE30/31 enables easy and stress free operation. The coaxial coarse/fine focusing knobs, controls for the stage and light intensity, are placed conveniently at your finger tips to minimize fatigue.


Light Source

The 6V/30W halogen Koehler illumination system provides bright, even illumination at any magnification. The "only one in its class" centerable lamp is housed externally and has an externally operated control mechanism for all facets of illumination. A segmented illumination intensity indicator is ideally located for easy viewing.


Observation Tubes

In order to maintain absolute parfocality, the Siedentopf eyepiece tubes will not change their length when interpupillary adjustments are made. The inclination of 45° for the binocular and trinocular tubes is chosen for comfort and posture management. Two trinocular versions are also available featuring professional light distribution sliders either for 100% binocular /100% photo tube or 100% binocular and 20/80% for binocular/photo. The trinocular heads are ideal for digital documentation and video projection.


Focusable Eyepieces

The standard Wide field 10x eyepieces for the AE30/31 with an enlarged field of view of 22mm provides enlarged viewing fields for faster scanning and easier viewing. Parfocality of focus is provided by independent diopter adjustment on each eyepiece. Users wearing eyeglasses will find the eyepieces' high eyepoint design very comfortable to use. Various graticules for measuring and counting are available for these eyepieces.


Quintuple Revolving Nosepiece

The revolving side facing nosepiece accepts five objectives. It runs on precision ball bearings and has internal positive click stops so that the image remains centered after each change in magnification. The internal components and the optics are sealed against fluid spills to ensure reliable and trouble-free usage. This allows the operator to concentrate fully on imaging the specimen and not to worry about accidentally damaging the microscope.


Objectives

The CCIS objectives for inverted microscopes have long free working distances in comparison to normal objectives of the same magnification. The objectives are optically corrected to compensate for 1.1mm thick glass slides and provide easy routine operation.

Phase Contrast Microscopy

Phase contrast is the most popular optical contrast method for viewing the detailed structure of unstained or living specimens. The Phase Plan Achromat objectives, coupled with the easy to operate phase slider, provide outstanding contrast for the most demanding applications. For a quick and easy change of magnification, two annular rings are provide on the non-centered phase slider. The center position on the slider is designated for bright field microscopy.

Condenser Mount

The centerable condenser mount is height adjustable with rack and pinion and is dovetail mounted on the illuminating pillar with a solid clamp screw. The ELWD condenser with a numerical aperture of 0.30 and a working distance of 72mm is suitable for objectives of magnification from 4x - 40x with an aperture diaphragm in the bright field Koehler illumination and for phase contrast.

The phase annular ring Ph1 for 10x and 20x and Ph3 for 40x are centered by Allen Hex keys on the centerable slider. The center position on each slider is designated for bright field usage.


Mechanical Stage and accessories

The standard stage is a fixed stage plate: the stage can be widened on both sides with auxiliary stage plates. A hard coating protects the stage surface from abrasion and wear. The tempered glass stage insert allows for checking the objective being used without removing the specimen from the stage.


An optional attachable mechanical stage with Well plate holder with low positioned coaxial controls is available. The mechanical stage also accepts interchangeable specimen holders for Petri dishes and glass slides.

Motic AE30 and AE31 Technical Specifications at a glance
Optical System Parfocal distance 45mm CCIS Optical System (Color Corrected Infinity System)
Observation tube

Siedentopf 45° Binocular (AE30) or Trinocular heads (AE31)

Eyepiece  Widefield WF 10x (FN22) with diopter adjustment
Nosepiece Quintuple reversed with splash protection
Coarse/Fine focusing  Coaxially positioned control knobs, coarse 42mm/rotation, fine 0.2mm/rotation. Min. increment 2µm. Coarse motion torque adjustable.
Stage Plain stage 200 x 260mm, hard coated surface.
Substage Illumination 6V/20W Quartz Halogen lamp in centerable housing and integrated diffuser
Condenser ELWD NA 0.30/72mm or LWD NA 050/28mm both focusable

Prod #DescriptionUnitPriceOrder / Quote
Complete Systems for the Motic AE30 and AE31
22434-10
AE30 Binocular Inverted Microscope, WF10x/22 Eyepieces, Plan Achromat PL4x Plan Achromat Phase 10x and 20x objectives, ELWD condenser, Phase slider, PH1, PH3 and phase centering telescope, plain stage, 6V/30W Quartz illumination
each
$2,885.00
Qty:
22434-12
AE31 Trinocular (80/20) Inverted Microscope, WF10x/22 Eyepieces, Plan Achromat PL4x Plan Achromat Phase 10x and 20x objectives, ELWD condenser, Phase slider, PH1, PH3 and phase centering telescope, plain stage, 6V/30W Quartz illumination
each
P.O.R.
Qty:
Main Body
22434-15
AE30 Main Body with quintuple nosepiece, plain stage, 45° binocular, Koehler illumination 6V/30W
each
1,638.00
Qty:
22434-16
AE31 (80/20) Main Body with quintuple nosepiece, plain stage, 45° trinocular, Koehler illumination 6V/30W
each
2,011.00
Qty:
all AE30/31 microscopes include 45mm blue, neutral density and green filter, power cord, allen hex key, vinyl dust cover. Universal power supply 100-240


Prod #DescriptionUnitPriceOrder / Quote
Accessories and Options for the Motic AE30 and AE31
Eyepieces (each)
22432-312
WF PL 10x (FN22), focusable with diopter adjustment
each
$79.00
Qty:
22432-313
WF PL 10x (FN22), with cross line, focusable with diopter adjustment
each
115.00
Qty:
22431-314
WF PL 12.5x (FN16), focusable with diopter adjustment
each
145.00
Qty:
22431-315
WF PL 15x (FN14.5), focusable with diopter adjustment
each
95.00
Qty:
Reticles (each)
22431-301
Cross Line Reticle, ø23mm
each
41.00
Qty:
22431-302
Cross Line Reticle with Micrometer Scale(100 div in 10mm), ø23mm
each
41.00
Qty:
22431-303
Micrometer Scale Reticle (100 div in 10mm), ø23mm
each
41.00
Qty:
Plan Achromat Objectives - PL INV
22434-350
CCIS Plan Achromatic Objective 4x/0.1
each
90.00
Qty:
22434-351
CCIS Plan Achromatic Objective 10x/0.25
each
111.00
Qty:
Plan Achromat Long Working Distance Objectives - PL LWD INV
22434-352
CCIS Plan Achromatic Objective 20x/0.4
each
221.00
Qty:
22434-353
CCIS Plan Achromatic Objective 40x/0.6
each
468.00
Qty:
22434-354
CCIS Plan Achromatic Objective 60x/0.7
each
1,008.00
Qty:
Plan Achromat Phase Long Working Distance Objectives - PL PH LWD INV
22434-345
CCIS Plan Achromat Phase Objective 10x/0.25
each
126.00
Qty:
22434-346
CCIS Plan Achromat Phase Objective 20x/0.40
each
289.00
Qty:
22434-347
CCIS Plan Achromat Phase Objective 40x/0.60
each
578.00
Qty:
Condensers
22434-372
ELWD NA0.30, WD=72mm Condenser
each
289.00
Qty:
22434-373
ELWD NA0.50, WD=28mm Condenser
each
499.00
Qty:
Phase Contrast Equipment
22434-365
Phase Slider, centerable PH1, BF, PH3 (for ELWD condenser)
each
126.00
Qty:
22434-366
Phase Slider, centerable PH1, BF, PH3 (for LWD condenser)
each
126.00
Qty:
22431-370
Phase Centering Telescope
each
121.00
Qty:
Stage Accessories
22434-440
Glass Insert
each
16.00
Qty:
22434-441
Metal Insert
each
32.00
Qty:
22434-442
Auxiliary Stages (paired)
each
54.00
Qty:
22434-443
Universal attachable mechanical stage with well plate holder (128 x 86mm)
each
583.00
Qty:
22434-444
Well Plate Holder (128 x 88mm)
each
73.00
Qty:
22434-445
35mm Petri Dish Holder
each
52.00
Qty:
22434-446
54mm Petri Dish Holder
each
52.00
Qty:
22434-447
65mm Petri Dish Holder
each
52.00
Qty:
22434-448
Hemacytometer Holder
each
52.00
Qty:
Photo and C-mount Video Adapters
22430-400
2.5x SLR projection lens, requires photo eyepiece
each
105.00
Qty:
22430-402
Photo Eyepiece 2.5x for photo adapter #22430-300
each
179.00
Qty:
22430-404
Photo Eyepiece 4x for photo adapter #22430-300
each
142.00
Qty:
22430-405
1.0x C-mount Camera Adapter (no lens)
each
132.00
Qty:
22430-406
0.65x C-mount Adapter for 2/3" chip sensors
each
132.00
Qty:
22430-407
0.5x C-mount camera adapter for 1/2" chip sensors
each
132.00
Qty:
Optional Accessories and Replacements
22431-422
Blue Filter (45mm)
each
10.00
Qty:
22431-423
Didymium Filter (45mm)
each
60.00
Qty:
22434-424
Neutral Density Filter ND-2 (45mm)
each
16.00
Qty:
22432-425
Green Interference Filter (45mm)
each
29.00
Qty:
22431-230
Quartz Halogen Lamp, 6V/30W
each
20.00
Qty:
EF-INV EPI Fluorescence Attachement
22434-500
Complete EPI-Fluorescence Attachement Kit, consists of the following individually listed parts
each
P.O.R.
Qty:
22434-510
EP-Fluorescence Attachement with Filter Cassette and Mercury Lamp
each
1,440.00
Qty:
22434-511
Lamphouse HBO 100W
each
294.00
Qty:
22434-512
Collector Lens
each
156.00
Qty:
22434-513
Mercury Lamp Socket for HBO 100W
each
575.00
Qty:
22434-514
Starter Unit HBO 100W
each
1,089.00
Qty:
22434-515
Lamp Centering Tool
each
103.00
Qty:
22434-516
Mercury Lamp HG-100W
each
510.00
Qty:
22434-517
UL/CSA Power Cord
each
12.00
Qty:
Standard Fluorescence Filter Sets
22434-520
DAPI and Hoechst set:
Exciter D350/50x
Dichroic 400DCLP
Emitter D460/50m
each
868.00
Qty:
22434-521
FITC/RSGFP/Fluo 3/Dio Acradine Orange (DNA) set:
Exciter D480/30x
Dichroic 505DCLP
Emitter D535/40m
each
868.00
Qty:
22434-522
TRITC (Rhodamine)/DiI/Cy3 set:
Exciter D540/25x
Dichroic 565DCLP
Emitter D605/55m
each
868.00
Qty:
22434-523
Texas Red/Cy3.5 set:
Exciter D560/40x
Dichroic 595DCLP
Emitter D630/60m
each
868.00
Qty:
22434-524
Cy5, Alexa Fluor 633, Alexa Fluor 647 set:
Exciter HQ620/60x
Dichroic Q660LP
Emitter HQ700/75m
each
865.00
Qty:
22434-525
Cyan GFP set:
Exciter D436/20x
Dichroic 455DCLP
Emitter D480/40m
each
868.00
Qty:
22434-526
Endow GFP Bandpass Emission set:
Exciter HQ470/40x
Dichroic Q495LP
Emitter HQ525/50m
each
1,035.00
Qty:
22434-527
Yellow GFP BP (10C/Topaz) set:
Exciter HQ500/20x
Dichroic Q515LP
Emitter HQ535/30m
each
885.00
Qty:
22434-550
Empty Filter Set
each
158.00
Qty:

Digital Cameras for AE31 Inverted Microscope #22434-12 and #22434-16

Requires CCD Adapter, Prod. No. 22430-407 (see above).

Prod #DescriptionUnitPriceOrder / Quote
Moticam Digital Cameras
2290-81
Moticam 1000, 1.3 Megapixel CCD Camera
each
$399.00
Qty:
2290-100
Moticam 2000, 2.0 Megapixel Digital Color Camera
each
699.00
Qty:
2290-120
Moticam 2300, 3.0 Megapixel Digital Color Camera
each
899.00
Qty:
2290-125
Moticam 2500, 5.0 Megapixel Digital Color Camera
each
1,295.00
Qty:

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