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PELCO easiSlicer™
McIlwain Tissue Chopper

Improved MicroSlicers™
for Sectioning Fresh or Fixed Tissue


NEW MicroSlicer™ DTK-1000N

NEW MicroSlicer™ Zero 1N

MicroSlicer™ DTK-3000W

The MicroSlicers™ allow to cut sections of soft tissues like brain, liver or similar difficult-to-section material. The MicroSlicers™ are well known for their quality and superior performance, enjoying an excellent reputation in the neuroscience research community.

The MicroSlicers™ have a unique design for the vibratory motion of the cutting blade. To generate the shuttling blade motion in the MicroSlicer™, a smooth motorized system was adopted that is less prone to vertical vibration, compared to the traditional electromagnetic vibrating system. Traditional electromagnetic system is limited to a set constant vibration frequency of the blade. In contrast, the MicroSlicer™ allows adjustment to the shuttling frequency of the blade depending on the properties of the specimen. Thus, the MicroSlicer™ can cut thinner sections more easily and efficiently.

Objective of the MicroSlicer™

The MicroSlicer™ is an instrument designed to cut sections of animal or plant tissues, fresh or fixed, without freezing them.

Various fields of neuroscience, histochemistry and cytochemistry require incubation of tissues in different incubation media. Large tissue blocks and thick sections often end up with non-specific deposits of reaction products and areas of no reaction in the center of the specimen. This is due to poor penetration of the reaction media into the tissue. For this reason, thinner sections are preferred to perform histochemical and cytochemical reactions.

The simplest method to cut tissue sections is to cut frozen in a cryostat. However, it is well known that both cellular fine structure and enzyme activity suffer greatly from freezing and thawing. Nonfrozen sections are therefore preferred for histochemistry and cytochemistry. In recent years, rat or cat fresh brain sections (200 to 500 micrometers in thickness) have been used in neurophysiological and pharmacological research. In addition, tissue sections are used in a variety of studies in the field of botany.

Traditionally, non-frozen tissue sections have been cut by hand with a razor blade or by using microtomes or tissue choppers. However, these methods have various drawbacks, such as the irregular thickness of tissue sections, difficulty of obtaining thin sections, or extremely slow sectioning speed. The MicroSlicers™ are instruments for preparing non-frozen tissue sections easily and efficiently.

There is a choice of three MicroSlicer™ models:

  • MicroSlicer DTK-1000N - the basic model for manual operation

  • MicroSlicer  Zero 1N - reduced Z-axis movement of the head for superior cutting quality, manual operation but includes motorized specimen movement and features the useful retraction mode

  • MicroSlicer DTK-3000W – fully automated system for serial sectioning

The MicroSlicer™ vibratory tissue slicer systems feature a fixed cutting amplitude and an adjustable frequency. Even the entry level model, DTK-1000N, comes equipped with an auto-reverse mechanism and detachable bath. In addition to that, the Zero 1N and DTK-3000W have a stepping motor for vertical movement of the specimen, as well as a retraction function. The Zero 1N model features the MicroSlicer™ Zero-Z technology with a redesigned cutting head for dramatically reduced Z-axis vibration. The layout of the switches on both sides of the tray allows for easy operation and reduced spillage on the controls.

Applications: Neuroscience Research, Physiology, Immunocytochemistry, Pharmacology, Pathology, Fluorescent Antibody Study, Histology, Cytology

Journal References to Research Work Done Using a MicroSlicer™

MicroSlicer™ Accessories and Consumables


microslicer dtk-1000

NEW MicroSlicer™ DTK-1000N

A robust, manual, entry-level microtome ideal for a busy laboratory with many users.

Functions Include:

  1. Frequency dial
  2. Cutting speed dial
  3. Function buttons; reverse; stop; forward
  4. Power button
  5. Specimen up/down dial
  6. Blade holder
  7. Specimen tray
  8. Ice bath

Ordering Information
Prod # Description Unit Price Order / Quote
10000N MicroSlicer™ DTK-1000N, 120V, 60Hz each P.O.R.
Qty:
10000N-220 MicroSlicer™ DTK-1000N, 220V, 50/60Hz each P.O.R.
Qty:




Microslicer™ DTK-Zero1

NEW MicroSlicer™ Zero 1N

the first vibratory slicer with Zero Z technology

This model MicroSlicer™ with Zero Z technology dramatically reduces the Z-axis vibrations to approximately 2.5 microns. It is also equipped with a specimen retraction system. The specimen advance is manual, but the specimen can be moved up or down by a motorized button. A function switch can stop, advance or retract the cutting action, saving valuable time.

  Functions Include:
 

1. Frequency dial
2. Cutting speed dial
3. Function buttons; reverse; stop; forward
4. Power button
5. Retraction button
6. Specimen up/down dial

 

7. Raise specimen button
8. Lower specimen button
9. Blade holder
10. Specimen tray
11. Ice bath

Ordering Information
Prod # Description Unit Price Order / Quote
10111N MicroSlicer™ Zero 1N, 115V, 50/60Hz each P.O.R.
Qty:
10111N-220 MicroSlicer™ Zero 1N, 220V, 50/60Hz each P.O.R.
Qty:




Microslicer™ DTK-3000W

MicroSlicer™ DTK-3000W

70 x 70mm maximum specimen size

This is the most sophisticated model. It is fully automated and can acommodate a specimen up to 70 x 70mm in size.

Functions Include:
Fully automatic single slice or continuous mode. Selection of section thickness is possible at one micron units with a high performance stepping motor for specimen advance. Equipped with a specimen retraction system. Forward and reverse blade movements are selectable at 1mm intervals.

  Functions Include:
 

1. Frequency dial
2. Cutting speed dial
3. Operation panel
4. Display (thickness)
5. Display (sectioning range)

 

6. Motor box
7. Blade holder
8. Buffer tray
9. Magnifier assembly with fluorescent lamp

Ordering Information
Prod # Description Unit Price Order / Quote
10150 MicroSlicer™ DTK-3000W, 115V, 50/60Hz each P.O.R.
Qty:








MicroSlicer™ Specifications Overview

Model Prod. No.
10000N and 10000N-220
MicroSlicer™
DTK-1000N
Prod. No.
10111N and 10111N-220
MicroSlicer™
Zero 1N
Prod. No.
10150
MicroSlicer™
DTK-3000W
Electrical 115V or 230V, 50/60Hz 115V or 230V, 50/60Hz 115V, 50/60Hz
Blade Oscillating Frequency 0~50Hz 0~50Hz 0~55Hz
Blade Oscillating Width 2mm fixed 2mm fixed 2mm fixed
Cutting Speed  0-68/mm/min 0-68/mm/min 0-90/mm/min
Reverse Speed 104mm/min 104mm/min 104mm/min
Maximum Specimen Height 20mm 20mm 20mm
Specimen Retraction Function no yes yes
Maximum Specimen Size 25 x 30mm 25 x 30mm 70 x 70mm
Specimen Advance Manual Motorized Motorized/Automatic
Blade Holder Size For 30mm blade For 30mm blade For 30 or 70mm blade
Automatic Operation no no yes

retraction mechanism

The retraction mechanism is a function included in the Zero 1N and DTK-3000W models that prevents the retracting blade from touching the face of the specimen. The specimen tray is lowered by 120 micrometers after the cutting of each section and is raised by exactly the same distance when a new cutting cycle starts.


MicroSlicer™ Accessories and Consumables

Microslicer™ Blade Holder

MicroSlicer™ Blade Holder

Black nylon and stainless steel

Fits MicroSlicers™ above. Does not fit MicroSlicer™ 1500E.

Prod # Description Unit Price Order / Quote
10110-20B MicroSlicer™ Blade Holder each $363.50
Qty:



tool for microslicer blade exchange

Tool for MicroSlicer™ Blade Exchange

Prod # Description Unit Price Order / Quote
10112 Tool for MicroSlicer™ Blade Exchange each $22.35
Qty:



microslicer bath for 10000N

NEW MicroSlicer™ Bath

Fits the new models DTK-1000N and Zero 1N. Does not fit DTK-3000W and older models. The removable bath can be chilled in a freezer for additional cooling power. Thus it is convenient to have more than one bath for the MicroSlicer™ DTK-1000N or Zero 1N .

Prod # Description Unit Price Order / Quote
10116N MicroSlicer™ Bath each P.O.R.
Qty:



microslicer tray for 10000N

NEW Specimen Tray for MicroSlicer™ DTK-1000N and Zero 1N

Fits the new models DTK-1000N and Zero 1N. Does not fit DTK-3000W and older models. The redesigned tray is deeper (15mm) and is attached to the instrument by magnetic force, allowing for easy and flexible orientation of the specimen for sectioning.

Prod # Description Unit Price Order / Quote
10113N Specimen Tray for MicroSlicer™ DTK-1000N and Zero 1N each P.O.R.
Qty:



Microslicer Specimen Tray

Specimen Tray for MicroSlicer™ DTK-3000W

Fits MicroSlicer™ DTK-3000W and older models.

Inside dimensions:
70 x 70 x 9.5mm
(2.75 x 2.75 x 0.375")

Prod # Description Unit Price Order / Quote
10113 Specimen Tray for MicroSlicer™ DTK-3000W each $295.00
Qty:




Feather Razor Blade for Vibratome use

Feather® Blades

Carbon steel; sharp edges. Break in half lengthwise for use (protect fingers and use eye protection). This is the preferred cutting blade for use with the PELCO easiSlicer™. Ideally suited for use with Microslicer™, Vibratome® and other vibratory slicers. Excellent when used as is (unbroken) in McIlwain Tissue Chopper.
• Length: 42.8mm (1.685")
• Cutting Edge Length: 36mm (1.42")
• Width: 22mm (0.865")
• Thickness: 0.127mm (0.0045")

Prod # Description Unit Price Order / Quote
121-9 Feather® Double Edge Carbon Steel Blades pkg/10 $8.25
Qty:




Double Edge, PTFE Coated Razor Blades for Vibratome

Double Edge, Breakable Style Razor Blades

PTFE-Coated stainless steel, individually wrapped and bulk packed. Break in half lengthwise for use (protect fingers and use eye protection).Suitable blade for PELCO easiSlicer™, Microslicer™, Vibratome® and other brands of vibratory microtomes. Suitable alternative to Feather® blades when used as is (unbroken) in McIlwain Tissue Chopper.
• Length: 42.9mm (1.69")
• Cutting Edge Length: 37.1mm (1.46")
• Width: 21.7mm (0.855")
• Thickness: 0.11mm (0.004")

Prod # Description Unit Price Order / Quote
121-6 Double Edge Stainless Steel Cutting Blades box/250 $39.50
Qty:




Replacement bulb for Microslicers

Replacement Twin Bulb

for Microslicer™ Models 1000 and 1500E

Prod # Description Unit Price Order / Quote
10174 Replacement, Twin Bulb for Microslicer™ models 1000, 1500E, Zero-1 and 3000W each $96.70
Qty:




PTFE coated, stainless steel, injector razor blades

Injector Style Stainless Steel, PTFE-Coated Blades

Acceptable for use with Microslicer™, Vibratome® and other vibratory microtomes.Single edge, 20 per dispenser.
• Length: 38.1mm (1.5")
• Cutting Edge Length: 36.3mm (1.43")
• Width: 8mm (0.314")
• Thickness: 0.25mm (0.010")

Prod # Description Unit Price Order / Quote
121-4 Injector Blades, 20 blades in dispenser each $4.90
Qty:




PELCO Pro CA44 Tissue Adhesive

PELCO® Pro CA44 Tissue Adhesive

PELCO® Pro CA44 is an instant tissue adhesive based on cyanoacrylate. Developed to glue tissue on specimen holders used in vibrating microtomes such as Microslicer™ and Vibratome®. Although the tissue adhesive is unstable in boiling water, it is recommended to use #19920 PELCO® Pro Cyanoacrylate Debonder (below) to remove or clean cured cyanoacrylate. Supplied in 20ml bottle with dispenser tip, closed with ptfe coated steel pin for optimum seal.
Compatible to Loctite 404 tissue adhesive.

Cyanoacrylate Brand Cross Reference and Technical Data
I - 10033 SDS (213KB PDF)

Prod # Description Unit Price Order / Quote
10033 PELCO® Pro CA44 Instant Tissue Adhesive, 20ml each $10.10
Qty:

The shelf life of cyanoacrylate glues (and epoxies) can be extended by storing them at lower temperatures; -20°C. At lower temperatures, the adhesive becomes very viscous and the speed of chemical reactions is practically reduced to zero. It will keep the adhesive stable for years. Before use, let the adhesive thaw with the container sealed to avoid condensation into the adhesive.





PELCO® Pro Cyanoacrylate Debonder

PELCO® Pro Cyanoacrylate Debonder

The PELCO® Pro Cyanoacrylate Debonder destabilizes and debonds cured cyanoacrylates. Useful for releasing specimens from holders, cleaning cyanoacrylates from surfaces and removing cured cyanoacylates from fingers. Works on all brands of cyanoacrylate glue (Loctite, Henkel, Permabond, 3M™, PELCO®, Aron Alpha, etc.). The debonder is also a solvent, please check before using on plastic surfaces / samples and avoid repeated skin contact. Supplied in 20ml bottle with dispenser tip.

F, Cg, I, T - SDS (96KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees

Prod # Description Unit Price Order / Quote
19920 PELCO® Pro Cyanoacrylate Debonder, 20ml each $5.95
Qty:
The shelf life of cyanoacrylate glues (and epoxies) can be extended by storing them at lower temperatures; -20°C. At lower temperatures, the adhesive becomes very viscous and the speed of chemical reactions is practically reduced to zero. It will keep the adhesive stable for years. Before use, let the adhesive thaw with the container sealed to avoid condensation into the adhesive.
Cyanoacrylate Brand Cross Reference and Technical Data






Loctite 404 Tissue Adhesive

Loctite® 404 Quick-Set

PELCO easiSlicer™ and other vibratory tissue slicing applications. Also for other quick-set tissue-slide uses.
0.33 oz. / 9.3g / 10ml
I - SDS (207KB PDF)

Prod # Description Unit Price Order / Quote
10035 Loctite® 404 Tisssue Adhesive, 9.3g, 10ml each $42.00
Qty:

(also see PELCO® Tissue Adhesive)





Specimen Retrievers

PELCO® Pro Specimen Retrievers

This is a perfect tool for retrieving free floating sections from the bath. Made of glass.

Prod # Description Unit Price Order / Quote
101-33 PELCO® Pro Specimen Retrievers pkg/5 $33.35
Qty: