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PAP Pens for Immunohistochemical Applications
and Section Adhesive Pens

PAP Pens
Paraffin Pen
Pen for Frozen Sections: Fro-Tissuer
Tissue Capture Pen
Grid Coating Pen: Coat-Quick "G"
ImmEdge™ Pen




hydrophobic barrier pen

Super PAP Pens: Liquid Blocker

General Use: Immunohistochemistry
Surfaces: Glass

ready-to-use hydrophobic barrier pens

PAP Pens are used for immunohistochemical applications. The hydrophobic properties allow the user to draw barriers on the slide, in any pattern, to confine the flow of reagents to a defined area. A minimum of valuable antiserum will be used. Visible marking is light green/blue and can be removed with Xylene.

For immunohistochemistry, the PAP Pen should be applied after deparaffinization.

Directions:
  1. Deparaffinize tissue sections and hydrate to water.
  2. Wipe away excess liquid around the section on the glass slide with tissue paper.
  3. Encircle the tissue section or draw lines on both sides of the section and let dry (10-15 seconds). Soak slides in PBS.
SDS (92KB PDF)
PELCO®Technical Notes, Super PAP Pen, Mini PAP Pen Liquid Blocker (PDF 87KB)

Prod # Description Unit Price Order / Quote
22309 Super PAP Pen Liquid Blocker, regular size, approximately 16.5ml each $60.50
Qty:
22311 Super PAP Pen Liquid Blocker, mini size, approximately 7.5ml each 45.45
Qty:




Adhesive Pen for Paraffin Sections

Paraffin Pen: Para-Tissuer

General Use: Histology
Surfaces: Glass

Adhesive pen for paraffin sections. A coating of the glass slide will prevent paraffin sections from falling off or wrinkling. Will flatten sections in room temperature water. Stable to 120° C.

PELCO®Technical Notes, Para-Tissuer, Adhesive Pen for Paraffin Sections (PDF 479KB)

MSDS (PDF 68KB)

Prod # Description Unit Price Order / Quote
22301 Adhesive Pen for Paraffin Sections each $91.75
Qty:




Adhesive Pen for Frozen Sections

Pen for Frozen Sections: Fro-Tissuer

General Use: Cryo Sectioning
Surfaces: Glass

Adhesive pen for frozen sections. Will prevent sections from falling off or wrinkling. Effective with PAP/PAAP, ABC, LAB-SA and immunofluorescent methods. Stable up to 110°C.

PELCO® Technical Notes, Fro-Tissuer, Adhesive Pen for Frozen Sections (PDF 479KB)

MSDS (PDF 68KB)

Prod # Description Unit Price Order / Quote
22302 Adhesive Pen for Frozen Sections each $91.75
Qty:




Adhesive Pen for Tissue Capture

Tissue Capture Pen

General Use: Histology
Surfaces: Glass

one pen for two applications

The new Tissue Capture Pen for use with frozen or paraffin sections. A coating of the glass slide will prevent the sections from falling off or wrinkling. Stable up to 110°C. Pen contains material for 3000-5000 applications.

PELCO® Technical Notes, Tissue Capture Pen (PDF 398kb)

Prod # Description Unit Price Order / Quote
22310 Adhesive Pen for Tissue Capture each $96.75
Qty:




grid coating pen

Grid Coating Pen: Coat-Quick "G"

General Use: TEM
Surfaces: TEM Grids - Copper, Molybdenum, Gold, Silver, Titanium, Nickel, Aluminum, Beryllium, Carbon

A light touch with this pen will provide a thin layer of adhesive on the grid. Layer will dry in 1-2 minutes at room temperature and is then ready to receive the sections. Pen may also be used as pretreatment before coating grids with formvar or other thin films.
MSDS (111KB PDF)

Prod # Description Unit Price Order / Quote
1556 Grid Coating Pen each $25.00
Qty:




ImmEdge Barrier Pen

ImmEdge™ Barrier Pen

General Use: Immunohistochemistry, In situ Hybridization
Surfaces: Glass

a hydrophobic barrier pen

The ImmEdge™ Pen, a hydrophobic barrier pen for immunohistochemistry and in situ hybridization detection methods, is designed for use with frozen or paraffin-embedded tissue sections mounted on glass slides. The ImmEdge™ Pen provides a water repellent barrier that keeps staining reagents localized on the tissue sections, prevents mixing of reagents when differentially staining two sections on the same slide, and allows the use of less reagents per section.

The boundary deposited by the ImmEdge™ Pen is lightly colored and easily visible. It is insoluble in alcohol and acetone, but is removed by most clearing agents. The ImmEdge™ Pen is compatible with all commonly used enzyme or fluorescent-based detection systems.

SDS (PDF 749KB)

Prod # Description Unit Price Order / Quote
22312 ImmEdge™ Barrier Pen each $108.50
Qty: