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Coating slide surfaces has been done with a variety of adhesives such as Poly-L-Lysine,
chrome alum and other materials but they are not suitable for immunogold labeling because of increased background
caused by attraction of gold particles to the slide’s adhesive.
The surface of glass slides is uneven and is activated by the silicon tetrahedral structure. Active sites are
then provided for adsorption of proteins or reactions with chemicals and reagents. A coating material of low
reactivity towards reagents minimizes this possibility. Biobond produces a very strong adhesion between
the glass and the tissue section for subsequent incubations.
Biobond coats a glass slide with a protective layer to minimize interaction of charged glass surfaces
with reagents. This is important for reproducibility of results because of variations that occur between glass
slides obtained from different sources and in different countries. It is very effective when used under severe
incubating conditions such as those encountered for in situ hybridization.
Biobond is suitable for all kinds of tissue specimens including paraffin wax or resin sections,
cell smears, cytospins or cryostat sections. It is supplied in 20ml volumes sufficient to coat at least 1,000
Detailed protocols provided.
General Use: Histology
PELCO®Technical Notes, Para-Tissuer, Adhesive Pen for Paraffin Sections (PDF 479KB)
MSDS (PDF 68KB)
General Use: Histology
one pen for two applications
General Use: TEM
Surfaces: TEM Grids - Copper, Molybdenum, Gold, Silver, Titanium, Nickel, Aluminum, Beryllium, Carbon
PELCO® Pro CA44 is an instant tissue adhesive based on cyanoacrylate. Developed
to glue tissue on specimen holders used in vibrating microtomes such as Microslicer™ and Vibratome®.
Although the tissue adhesive is unstable in boiling water, it is
recommended to use #19920 PELCO® Pro Cyanoacrylate Debonder (below) to remove
or clean cured cyanoacrylate. Supplied in 20ml bottle with dispenser tip, closed
with ptfe coated steel pin for optimum seal.
Compatible to Loctite 404 tissue adhesive.
Cyanoacrylate Brand Cross Reference and Technical Data
I - 10033 SDS (213KB PDF)
The shelf life of cyanoacrylate glues (and epoxies) can be extended by storing them at lower temperatures;
-20°C. At lower temperatures, the adhesive becomes very viscous and the speed of chemical reactions is
practically reduced to zero. It will keep the adhesive stable for years. Before use, let the adhesive thaw
with the container sealed to avoid condensation into the adhesive.