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Histology Adhesive

Biobond™ Tissue Section Adhesive

slide coating with advantages, especially for immunolabeling

Coating slide surfaces has been done with a variety of adhesives such as Poly-L-Lysine, chrome alum and other materials but they are not suitable for immunogold labeling because of increased background caused by attraction of gold particles to the slide’s adhesive.

The surface of glass slides is uneven and is activated by the silicon tetrahedral structure. Active sites are then provided for adsorption of proteins or reactions with chemicals and reagents. A coating material of low reactivity towards reagents minimizes this possibility. Biobond™ produces a very strong adhesion between the glass and the tissue section for subsequent incubations.

Biobond™ coats a glass slide with a protective layer to minimize interaction of charged glass surfaces with reagents. This is important for reproducibility of results because of variations that occur between glass slides obtained from different sources and in different countries. It is very effective when used under severe incubating conditions such as those encountered for in situ hybridization.

Biobond™ is suitable for all kinds of tissue specimens including paraffin wax or resin sections, cell smears, cytospins or cryostat sections. It is supplied in 20ml volumes sufficient to coat at least 1,000 slides.
Detailed protocols provided.

I - MSDS - Hazard Shipping Fees
PELCO® Technical Notes, BIOBOND™ Tissue Section Adhesive (20ml) Prod. No. 15715 (PDF 91K)

Prod # Description Unit Price Order / Quote

15715
Biobond™ Tissue Adhesive, 20ml
each
$62.00

Qty:

Citifluor Antifadent Solutions

citifluor fluorescent antifade mounting medium

The use of fluorescent labels and markers is now widespread and immuno-fluorescence, which relies on the use of antibodies tagged with labels such as fluorescein, is a well-established technique.

A common problem associated with the microscopical examination of these materials is that the illumination used for stimulating the fluorescence also causes degradation of the label and this causes fading of fluorescence. This also is a problem when trying to visualize materials having a low level of labeling. The photofading of labeled materials can be retarded by the use of the Citifluor mountants.

Antifadent (AF) Solutions
The antifadent solutions AF-1, AF-2 and AF-3 were specifically developed for use with FITC polyclonal antibodies but their use is now far more widespread. eservation with many other fluorochromes including rhodamines and DAPI. AF4 contains the antifadent propyl gallate, which is particularly suitable for use with DAPI and Alexa dye-stained materials, as well as FITC-labelled materials.

AF-1 - Glycerol-phosphate buffered solution containing antifadent for use with labeled tissue and dead cells. Available in two sizes.

Prod # Description Unit Price Order / Quote

Citifluor Antifadent (AF) Solutions

19470
AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 25ml
each
$76.50

Qty:

19470-1
AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 100ml
each
198.00

Qty:

I - MSDS (pdf 109K)

19472
AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 25ml
each
76.50

Qty:

19472-1
AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 100ml
each
198.00

Qty:

I - MSDS (pdf 109K)
Glycerol solution, additives.

19474
AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 25ml
each
76.50

Qty:

19474-1
AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 100ml
each
198.00

Qty:

I - MSDS(pdf 108K)

19476-A
Citifluor non-fluorescent Immersion Oil, AF87, 10ml
each
68.50

Qty:

I - MSDS(pdf 107K)

19476
AF-4, Glycerol solution containing n-propyl gallate for use with labelled tissue sections and dead cells, 25ml
each
76.50

Qty:

19490
AF-100, Phosphate-buffered saline solution containing antifadent specially prepared for use with UVM23 solution where fluorochrome fading is a problem, 5ml
each
15.20

Qty:

I - MSDS (pdf 109K)

19491
AF-200, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used with other fluorochromes such as fluorescein, Alexa dyes and Hoescht dye, 10ml
each
68.50

Qty:

I - MSDS (pdf 108K)

19492
AF-300, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used where fluorescein is the staining material. The solution can also be used with other fluorochromes such as rhodamines, etc., 10ml
each
68.50

Qty:

I - MSDS (pdf 109K)

19493
CFM1, Glycerol-PBS ref. index similar to glass, 10ml
each
45.50

Qty:

I - MSDS (pdf 109K)

19494
CFM1, Glycerol-PBS w/AF, ref. index similar to glass, 10ml
each
52.70

Qty:

I - MSDS (pdf 135K)

19495
CFM2, Glycerol-TRIS buffer, ref. index similar to glass, 10ml
each
45.50

Qty:

I - MSDS (pdf 110K)

19496
CFM3, Glycerol-Phosphate & AF, ref. index similar to glass, 10ml
each
52.70

Qty:

I - MSDS (pdf 109K)

UV Mount Media (UVM)

19497
UVM1, Methacrylate based solution, UV curable, gives clear film, 10ml
each
45.50

Qty:

I - MSDS (pdf 69K)

19498
UVM2, Methacrylate based solution, contains anti-bleaching agent, 10ml
each
45.50

Qty:

I - MSDS (pdf 90K)

19499
UVM3, High refractive index methacrylate-based solution designed to be used with AF-100 where fluorochrome fading is a problem, 10ml
each
62.35

Qty:

I - MSDS (pdf 68K)

Solid Mountants

19469
Citifluor Solid Mountant Kit is comprised of PVA solution, 25ml and Citifluor antifadent in buffer, 5ml. The components can be mixed as required, applied to the specimen and allowed to dry to give a clear film, 30ml
each
95.00

Qty:

C - MSDS for Citifluor AF100 Antifadent Solution, 25ml (pdf 183K)

U - MSDS for Citifluor CFPVOH Solution, 5ml (pdf 176K)

Contains anti-fading solution. Suitable for preventing fading of dyes such as DAPI which have the tendency to disperse in glycerol based mounting solutions.
Colloidal Adhesives: See "Adhesives"

Background Buster

Peptide blocker for removal of background staining

For use in: Immunohistochemistry, Immunofluorescence, In-situ probe staining

Background staining or non specific staining is a common problem in immunohistochemistry, immunofluorescence and in-situ probe staining reducing the signal to noise ratio. Background Buster is a peptide blocker that eradicates all general background staining caused by primary antibodies, staining reagents, chromogens, fixatives, and endogenous biotin. Background Buster is used in place of normal sera and other blocking agents for removing background in both animal and human tissue staining. Eradicating background, thereby increasing the signal to noise ratio is important for obtaining background free specific staining for the ease of qualitative and quantitative evaluation of staining procedures.

Note: Nonspecific staining due to red blood cells is caused by endogenous peroxide enzyme in the red blood cells. This type of background requires a pre-treatment step with 3% hydrogen peroxide in water. This blocking step should precede the blocking step with Background Buster.

Prod # Description Unit Price Order / Quote

18722-50
Background Buster, 50ml
each
$289.50

Qty:

18722-125
Background Buster, 125ml
each
417.90

Qty: