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Histology Overview
Biological Microscopes
Calibration Overview

Stage Micrometers for Transmitted and Reflected Light

Stage Micrometers for Transmitted Light

These accurate stage micrometers with optional certificates are intended for routine calibration of light microscopes, particularly when alternating objectives of different microscopes. Ideal for student, lab settings and instructional purposes. Robust construction. Scale is centered on a glass disc, mounted in a black anodized aluminum slide, 75 x 24 x 2mm thick.
Table of Accuracy Optional calibration certificates

Prod # Description Unit Price Order / Quote

2280-10
Micrometer Scale 10mm in 0.1mm divisions
each
$168.40

Qty:

2280-11
Micrometer Scale 5mm in 0.05mm divisions
each
198.50

Qty:

2280-12
Micrometer Scale 0.1 inch in 0.001 inch divisions
each
198.50

Qty:

2280-13
Micrometer Scale 1mm in 0.01mm divisions
each
168.40

Qty:

2280-14
Micrometer Scale 0.005 inch in 0.0001 inch divisions
each
292.50

Qty:

2280-15
Micrometer Scale 0.1mm in 0.002mm divisions
each
292.50

Qty:

2280-16
Crossed Micrometer Scales, 1mm in 0.01mm divisions
each
244.50

Qty:

2280-17
Micrometer Scale Vertical, 2mm in 0.01mm divisions
each
198.50

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2280-18
Micrometer Scale 1mm in 0.01mm divisions, no coverglass
each
198.50

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Line Width and Accuracy of Stage Micrometers

Product #	Line Width	Accuracy (overall)
2280-10	0.005 mm	within 0.002 mm
2280-11	0.005 mm	within 0.0015 mm
2280-12	0.002 mm	within 0.0001 inch
2280-13	0.002 mm	within 0.001 mm
2280-14	0.001 mm	within 0.00005 inch
2280-15	0.001 mm	within 0.001 mm
2280-16	0.0015 mm	within 0.001 mm
2280-17	0.0025 mm	within 0.0015 mm
2280-18	0.0027 mm	within 0.001 mm
2280-24	0.002 mm	within 0.001 mm
2269-10	0.003 mm	within 0.001 mm

Combined Metric/Inch Stage Micrometer

2mm in 0.01mm divisions and 0.1 inch in 0.005 inch divisions.
Table of Accuracy Optional calibration certificates

Prod # Description Unit Price Order / Quote

2280-24
Stage Micrometer, metric/inch, 2mm/0.01mm and 0.1"/0.005"
each
$278.25

Qty:

Grouped Graduation Pattern Stage Micrometer

For speedy determination of a range of feature sizes from 0.01 up to 5mm within a given specimen. Optional certificate available.

Patterns are 0.2mm in 0.01mm divisions, 2mm in 0.2mm divisions, and 5mm in 0.5mm divisions.
Table of Accuracy Optional calibration certificates

Prod # Description Unit Price Order / Quote

2280-25
Stage Micrometer Grouped Pattern
each
$198.50

Qty:

Stage Micrometers for Reflected Light

This accurate scale is etched through a highly reflective vacuum coated metal. When viewed with reflected light illumination, as with a metallurgical microscope, the scale appears black against a bright background.
Table of Accuracy Optional calibration certificates

Prod # Description Unit Price Order / Quote

2280-27
Micrometer Scale for Reflected Light, 10mm in 0.1mm divisions
each
$198.50

Qty:

2269-10
Micrometer Scale for Reflected Light, 1mm in 0.01mm divisions
each
198.50

Qty:

Calibration Certificates

For the above stage micrometers, both for transmitted and reflected light, certificates are

ONLY AVAILABLE AT TIME OF PURCHASE.

Available Certificate Types:

GRATS Certificate (Graticules Measurement) (#2280-60)
This is a manufacturer calibration. For applications which do not require the accuracy provided by calibrations carried out by NPL or UKAS, Graticules/Pyser-SGI provides a Certificate of Comparison for the scale which is compared with an NPL calibrated in house standards and a statement is provided on the accuracy of the scale with respect to these standards.

UKAS Cerificate (#2280-61)
A UKAS accredited laboratory carries out measurements at selected points on the scales and issues a calibration certificate which is internationally recognized.

NPL Calibration (#2280-62)
This is carried out by the National Physical Laboratory. NPL carries out measurement at selected points on the scales and grids and issues a certificate of calibration which is internationally recognised.

Prod # Description Unit Price Order / Quote

2280-60
GRATS Certificate done by the manufacturer
each
$450.20

Qty:

2280-61
UKAS Certificate Traceable to UKAS Standard
each
510.50

Qty:

2280-62
NPL Certificate Traceable to NPL (National Physics Laboratory, counterpart of NIST in the UK.)
each
1,075.00

Qty:

Image Analysis Standard (Reference Stage Graticule)

Provides 4, high precision test areas designed for calibrating image analysis systems and identifying deviations and distortions in optical imaging systems.

The standard, which can also be used as a high precision stage micrometer, is supplied with recommendations for its use and an individual certificate of calibration.

It is produced on a 75mm x 25mm slide and has a square grid accuracy of ±0.1µm and a dot accuracy of ±0.3µm (except for the smallest and largest two dots on the root-2 array where accuracy is ±0.5µm).

The four test areas are:

A 400µm x 400µm square grid which is subdivided into 200, 100, 50 and 25µm squares provides a means so detecting gross image distortions and can be used as an accurate two dimensional stage micrometer.
A 20 x 17 array of nominally 15µm diameter dots can be used to identify lens distortions. i.e. to set the field of view to eliminate edge distortion.
A root-2 array of spots from 3µm to 48µm diameter is used for determining the threshold level of cameras and microscope systems.
A log-nominal distribution array of 100 spots ranging from 4.5µm to 27µm diameter enables the mean and standard deviation to be determined and compared with the certified values. This is an idealized distribution of maximum dynamic range for a full screen.

Supplied with a Certificate of Calibration from NPL (National Physics Laboratory, counterpart of NIST in the UK.).

Note: NPL is part of the International Metrology Group so that all of its measurements are directly traceable to the International Metre Standard held in Paris, France. This means that the certificate is internationally traceable.

Prod # Description Unit Price Order / Quote

2280-26
Image Analysis Standard Slide
each
P.O.R.

Qty:

Counting Slides

Prod # Description Unit Price Order / Quote

2280-30
Counting Slide, 1mm Square / 0.1x0.1mm
each
$212.40

Qty:

2280-31
Counting Slide, .5mm Square / 0.05x0.05mm
each
212.40

Qty:

Counting Grids

Prod # Description Unit Price Order / Quote

2280-32
Counting Grid, 0.01mm grid / 0.2x0.2mm overall
each
$220.50

Qty:

2280-33
Counting Grid, 0.01mm grid / 1.5x1.5mm overall
each
220.50

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England Finder - S7 Slide

Locator and Calibration Slide

England Finder Slide
Progressively enlarged view of slide surface.

X (A through Z, excluding I) Y (1 through 75), note K34 to right.

The England Finder is a glass slide marked over the top surface in a way that a referenced position can be directly read relative to the locating edges.
All England Finders produced by Graticules for over 40 years are identical. The prupose of the finder is to give the microscopist an easy method of recording the position of a particular field of interest, so that the same position can be re-located at a later date, or by another person in another laboratory, or when using any other England Finder on any other microscope.
The location of the arrows is identical for all England Finder slides. Mark a label on the left hand side of the specimen slide, indicating the orientation to be repeated. By replacing the specimen slide with the England Finder, taking care not to disturb the position, the feature of interest can be noted. The feature an also be re-located at another place or time by reversing the procedure. Over 1,800 positions on a 76 x 26mm slide can be accurately located. Slide thickness is 1.35mm ±0.05mm. Nominal line width is 0.025mm.

Prod # Description Unit Price Order / Quote

2280-50
England Finder S7 Slide
each
$225.50

Qty: