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Technical Information on Gold Conjugates
back to Immunogold Stains, page 1

Nanometer-sized gold and silver particles of uniform shape and size are invaluable tools in nanotechnology (assembled arrays), light scattering (elastic - plasmon resonance; inelastic - Raman effect) and for single molecule detection.  Gold nanoparticles can be attached to proteins, alkanethiols and DNA by various methods.  Silver nanoparticles are evolving similar functional characteristics to their gold counterparts.
Ted Pella, Inc. is the leader in offering a wide range of both silver and gold nanoparticles for these applications.  Talk to our applications engineer to find out about the new developments/applications.  We are constantly developing new gold and silver  colloids for new nanotechnology applications.

The nanometer sized gold colloids can be characterized as follows:

• Monodispersed gold nanoparticles supplied in water, having trace amounts of citrate, tannic acid and potassium carbonate
• Citrate stabilized with a net negative surface charge (Langmuir 2005, 21:9303-9307)
• Gold colloid pH's range from above 5 for 5nm gold to less than 9 for the larger sized particles (pH increases with size).
• Available in a size range from 2 to 250nm
• The particles are invaluable tools for light scattering, either Raman or plasmon resonance (Analytical Chemistry 1999, 71:4903-4908; Analytical BioChemistry 1998, 262:137-156)

The silver colloids are a newer offering and have not been characterized as well as the gold colloids. They are supplied in water, have a negative surface charge and are available in 4 sizes from 20 to 80nm.

These products are known to be stable for a minimum of one month after opening. To avoid contamination after opening, it is best to handle the product under clean room conditions to avoid contamination by dust or other airborne contaminants. Temperatures for storage, after opening, can vary from 4-8°C (39-46°F) to room temperature depending on whether the application is diagnostic or research oriented. The colloid is stable on boiling and destroyed by freezing. When storing the product below 8°C (46°F) it is the customer's responsibility to ensure that their refrigerator can maintain a constant temperature in the range noted above.

NOTE: To best insure product quality, all shipments of unconjugated gold and silver colloids are sent for next day delivery. No Friday (weekend) shipments.

Gold Colloids (Sols)

MSDS Material Safety Data Sheet for Unconjugated Gold Sols

Size (nm)	Particles/ml		20 ml	Price $62.00/ea		100 ml	Price $250.00/ea		500 ml	Price $825.00/ea
2nm	1.5 x 1014		15701-20	Qty:		15701-1	Qty:		15701-5	Qty:
5nm	5.0 x 1013		15702-20	Qty:		15702-1	Qty:		15702-5	Qty:
10nm	5.7 x 1012		15703-20	Qty:		15703-1	Qty:		15703-5	Qty:
15nm	1.4 x 1012		15704-20	Qty:		15704-1	Qty:		15704-5	Qty:
20nm	7.0 x 1011		15705-20	Qty:		15705-1	Qty:		15705-5	Qty:
30nm	2.0 x 1011		15706-20	Qty:		15706-1	Qty:		15706-5	Qty:
40nm	9.0 x 1010		15707-20	Qty:		15707-1	Qty:		15707-5	Qty:
50nm	4.5 x 1010		15708-20	Qty:		15708-5	Qty:		15708-55	Qty:
60nm	2.6 x 1010		15709-20	Qty:		15708-6	Qty:		15708-65	Qty:
80nm	1.1 x 1010		15710-20	Qty:		15708-8	Qty:		15708-85	Qty:
100nm	5.6 x 109		15711-20	Qty:		15708-9	Qty:		15708-95	Qty:
150nm	1.7 x 109		15712-20	Qty:		15709-10	Qty:		15709-105	Qty:
200nm	7.0 x 108		15713-20	Qty:		15709-11	Qty:		15709-115	Qty:
250nm	3.6 x 108		15714-20	Qty:		15709-12	Qty:		15709-125	Qty:

Silver Colloids (Sols)

MSDS Material Safety Data Sheet for Silver Colloids

Size (nm)	Particles/ml		20 ml	Price $62.00/ea		100 ml	Price 250.00/ea		500 ml	Price 825.00/ea
20nm	7.0 x 1011		15705-20SC	Qty:		15705-1SC	Qty:		15705-5SC	Qty:
40nm	9.0 x 1010		15707-20SC	Qty:		15707-1SC	Qty:		15707-5SC	Qty:
60nm	2.6 x 1010		15709-20SC	Qty:		15708-6SC	Qty:		15708-65SC	Qty:
80nm	1.1 x 1010		15710-20SC	Qty:		15708-8SC	Qty:		15708-85SC	Qty: