All of the solutions below are produced exclusively by BBI, British BioCell International.
Gold Conjugates for Electron Microscopy,
and Lateral Flow Applications
British BioCell International (BBI) gold conjugates consist of the finest infinity
purified antibodies conjugated to gold nanoparticles manufactured to demanding size
and shape specifications.
Conjugates are available for the following immunolabeling applications:
- Electron microscopy - cryoultramicrotomy, freeze fracture or plastic sections, paraffin, cryostat or vibratome
- Lateral Flow Applications - Diagnostic test kits
The gold conjugates are made to the highest standards and specifications, yielding excellent
results when correctly used. All gold conjugates are supplied in one of the two buffers
listed below. Buffer constituents
dictate the shelf life and storage conditions for the individual conjugates.
Gold conjugates for electron microscopy (EM) are supplied in the following buffer:
20mM Tris (tris-hydroxymethyl-aminomethane); 20mM sodium azide; 154mM NaCl; 1% BSA; 20% glycerol; pH 8.2.
Recipe to make 100ml:
0.242g (20mM) Tris + 0.9g (154mM) NaCl + ultrapure water to make 100ml. Adjust pH
from 7.2 to 8.2 with 1N HCl or 1N NaOH.
Storage: Stable for 1 year at 4°C; stability for 2+ years at 20°C.
The conjugates demonstrate remarkable
stability at ambient temperatures for up to 7 days. Repeat freezing and thawing is not recommended.
Gold conjugates for lateral flow applications are supplied in the following buffer:
2mM sodium tetraborate at pH 8.2
containing 0.095% sodium azide.
Storage: Stable for 1-2 years at 4-8°C. DO NOT FREEZE. The conjugates demonstrate
remarkable stability at ambient
temperatures for up to 7 days.
Each gold conjugate has a technical data sheet which indicates the following information:
1) Number of particles counted;
2) Mean particle diameter; 3) Coefficient of variation given as a percent; 4) Percent
of single particles; 5) Percent of
particles larger than triplets; and 6) Minimum detectable protein. The coefficient
of variation is an important parameter
in describing the relative distribution of gold particle sizes around the mean for
a given batch. The coefficient of variation
equals the standard deviation divided by the mean.
Normal Gaussian distributions work as follows: ±1 standard deviation describes
68% of the area under the curve; ±
2 standard deviations describe 95% of the area under the curve; ± 3 standard
deviations describe 99.73% of the area under
the curve. As an example, you have purchased a gold conjugate - Goat anti-Rabbit
IgG (H+L), 10nm - having a mean particle diameter
of 9.8nm with a coefficient of variation of 4.1%. First, the standard deviation needs
to be determined. In this case it is 0.402nm
(4.1% x mean particle diameter). Statistically, 68% of the particles will be from
9.40 to 10.20nm, 95% from 9.00 to 10.60nm and 99.73%
from 8.60 to 11.00nm. A reliable size characterization has been determined for the batch.
Link to mean recommended working dilutions and technical information.
All shipments of Gold Conjugate products must be for next day delivery due to temperature
No Friday (weekend) shipments.
Explanations of Abbreviations
binds with heavy and light chain of primary antibody
binds with heavy chain only of primary antibody
conjugate absorbed against human serum proteins
conjugate absorbed against rat serum proteins
conjugate absorbed against mouse serum proteins
conjugate contains both Fab subunits (no Fc subunit) of the antibody