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Stains are classified as acidic, basic or neutral, depending on the nature of the stain's colored radicals. Acidic stains tend to react with cytoplasm, whereas basic stains are taken up by nucleic acids. In electron microscopy, the term "staining" may be applied to procedures wherein electron-dense materials such as salts or osmium, uranium and lead bind to and increase the electron density of various cell constituents.
For example, Aniline Blue is an acidic, water-soluble dye used to stain connective tissue. This dye is valuable for visualizing connective tissue fibers beneath epithelia within glands and muscles. Aniline Blue colors collagen fiber dark blue, mucous and cartilage matrix light blue, and nerve tissue lilac. It can be used as a counterstain with red nuclear dye. Concentrations as low as +/-1% can be used to evaluate thick sections of resin-embedded material for electron microscopy.
Dishes, covers and racks are manufactured from soda-lime glass and resist stains from Eosin or Hemotoxylin. Staining dishes are available in many configurations to accommodate up to 50 slides, with various glass and stainless steel racks.
Coplin jars can be used for pre-staining with and alcohol buffer. Slides are held vertically for easy manipulation. A Coplin jar can also serve as a preparation chamber for micro-thin-layer chromatography.
Wheaton staining dishes have been used for a variety of procedures to clean slides and coat them with a variety of materials (for example, Bain, J.R.; Hoffman, A.S. Glycophase glass revisited: Protein adsorption and cell growth on glass surfaces bearing immobilized monosaccharides, Biomaterials, in press 2002). Wheaton staining dishes have also been used for poly lysine and silane slide coatings.
Note: Wheaton staining dishes are not autoclavable or microwaveable, and are not intended for use at temperatures below 15°C or above 80°C.
Wheaton Staining Dish
These mix-and-match components offer greater flexibility in meeting your requirements. This staining dish accommodates 16, 30 and 40 slide racks, listed below. Manufactured from Wheaton 810 soda lime glass.
30-Slide Rack, Stainless SteelSlotted rack is designed to hold 30 microscope slides 3 x 1" (75 x 25mm), 3 x 1-1/2" (75 x 38mm), and 3 x 2" (75 x 51mm). The rack is made of non-tarnishable stainless steel and is resistant, permanently attached, but hinged to permit closure of dish and easy insertion and removal of the microscope slides.
16-Slide Rack, Glass
The Glass Rack holds 16 single slides, 31 slides arranged alternately straight across and diagonally, or 32 slides back-to-back.
40-Slide Rack, GlassHolds 20 single slides or 40 back to back of regular 3 x 1" (75 x 25mm) size, 3 x 1-1/2" (75 x 38mm) and 3 x 2" (75 x 51mm) sizes.
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Vertical Staining Jar with CoverThe wide top makes this jar especially suitable for staining slides inscribed on one end. Holds 8 single 3 x 1” (75 x 25mm) slides vertically or 16 slides back-to-back. Made of “400” borosilicate glass. Approximate inside dimensions: 90 x 50 x 30mm (3.5 x 2 x 1.18") |
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Hellendahl Type Staining DishFor staining or use as a TLC developing chamber. Holds 8 individual 3 x 1" (75 x 25mm) slides. If necessary, 16 slides can be accommodated back-to-back. Made of soda lime glass. Approximate inside dimensions: 75 x 80 x 30mm (3 x 3.14 x 1.18") |
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Coplin Staining Jar with Glass CoverA popular staining jar with heavy glass walls and broad base for increased stability. Holds 5 single 3 x 1" (75 x 25mm) slides verticallyor 10 slides back to back. Requires small reagent volume. Made of “800” soda lime glass. Approximate inside dimensions: 75 x 30 x 30mm (3 x 1.18 x 1.18"). |
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Short Coplin Staining Jar with Plastic Screw TopHolds 5 single 3 x 1" (75 x 25mm) slides or 10 back to back. Slides extend above the opening, permitting them to be manipulated without forceps. Screw cap is linerless, white polypropylene. The white linerless polypropylene Screw cap closure retains organic solvents and reduces evaporation. Rectangular base. Made of “800” soda lime glass. Approximate inside dimensions: 65 x 30 x 30mm (2.6 x 1.18 x 1.18") |
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Tall Coplin Staining Jar with Plastic Screw TopFor staining slides or use as a developing chamber in thin layer chromatography. Unit holds 5 single 3 x 1" (75 x 25mm) slides vertically or 10 slides back-to-back. Screw cap is white linerless polypropylene which reduces solvent evaporation. Rectangular base. Made of “800” soda lime glass. Approximate inside dimensions: 70 x 30 x 30mm (2.75 x 1.18 x 1.18") |
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Plastic Slide GripHolds five each 3 x 1" (25 x 75mm) slides and fits Coplin Jars and round-mouthed staining jars. Molded plastic withstands chemicals used in staining including special stains and metals. Can be used in temperatures up to 80° C. |
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Coplin Staining Jar with Tall Glass CoverChamber with extra tall lid accepts five single 3 x 1" (75 x 25mm) and 3.94 x 1" (100 x 25mm) slides. Made of “400” borosilicate glass. Meets USP Type 1 standards. |
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Polypropylene Staining JarTapered, square with flat shallow threaded screw cap. Holds 10 standard 3 x 1" (75 x 25mm) slides back-to-back. Loosen lid before autoclaving. |
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Coplin Staining JarPolypropylene staining jar holds either 5 single or 10 back to back standard 3" x 1" (75 x 25mm) slides. The interior is grooved to hold the slides in and upright position. Domed and shallow thread cap provides protection to the slides. Loosen lid before autoclaving. Sold in package of 4. |
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Polypropylene Staining Jar
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Polypropylene Staining Jar
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Mini-Stainermade of polypropylene, is microwave compatible, saves staining reagents |
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Coverglass Staining JarGlass jar with ground glass cover, accommodates 8 each back-to-back size 18 x 18mm coverslips. 45mm high. |
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Columbia Staining Jar for CoverslipsThis staining jar can hold up to 4 cover slips measuring 17-23mm wide, and up to 30mm long. Longer coverslips can be accommodated by removing the cap. The jar comes with a white polypropylene screw cap with a PTFE coated polyethylene liner. It is manufactured from Wheaton "800" glass, which meets USP type III standards, and is resistant to most chemicals. |
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Microwave-assisted Immunostaining on Glass Slidesusing the Sequenza Slide Rack and Coverplate system
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Immunostain Moisture ChamberThis is a low-cost, high precision moisture chamber for ten slides. Every moisture chamber is divided into ten individual compartments with approximately 12.7mm (1/2") empty space between the compartments. When the chamber lid is closed, eight barrier dividers are placed into the empty spaces between the microscope slide compartments completely isolating all compartments. This is a very desirable feature when doing immunostaining. |
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![]() ![]() Color coded Capinserts™ ![]() Bottom view |
LockMailer™ - Microscope Mailer and Staining JarThe LockMailer™ is a tamperproof multi purpose compact container for transporting, storing, mailing or staining microscope slides. It is constructed of extra-strong and clear polypropylene and will hold up to 4 standard 1x3" or 25x75mm slides vertically. The inside slotted channels will keep the slide safely separated. It incorporates a unique tamper evident leakproof screw cap made of HD polyethylene. The tamper evident screw cap avoids manipulation of slides without your knowledge. The LockMailer™ can also be used without the tamperproof feature. The caps on the Lockmailer™ can be color coded with the optional Capinserts™, available in different colors: blue, gray, green, lilac and orange. The Capinsert™ is inserted in the top of the cap and has a write frosted area for sample identification.
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Slide Staining and Storage Systemstain, file and storeUniversal slide rack and container for autoradiography gel staining and staining techniques. Molded of black polyoxymethylene-poly-acetal plastic which has great advantages over glass in all cytological and histological laboratories. |
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| The rack and dish are easy to clean and resistant to chemicals used in staining procedures. Slide Rack The slide rack has an open bottom for rapid draining. It holds 25 x 15mm (1" x 3") slides in slots numbered 1 to 25. The handle folds flat on either side. Four of these racks fit into the Slide Storage Box (see below). Maximum use temperature 90°C (194°F).
Staining Dish
![]() Storage Box A black polystyrene storage box (not for staining) holds four Slide Racks (Prod. No. 21078 above), not included. Provides convenient, indexed storage for the slide racks. There is a paper index for each of the four racks and also a paper index inside the cover that marks each section numerically 1 to 25. The box measures 19 x 17 x 5cm high (7-1/2" x 6-5/8" x 1-1/2").
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