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Cargille Microscope Immersion Oils
for light microscopy, fluorescence microscopy, inclined instruments and projection equipment
Cargille Immersion Oils are in full accord with the FDA requirements.
To date, no other immersion oils meet the FDA criterias.
Cargille Immersion Oils are PCB (polychlorinated biphenyls) free.
Ted Pella, Inc. offers the four standard types of immersion oils:
Type A, Type B, Type NVH and Type 300 (in 5- pack).
Which Type - Which Application?
For Normal Light Microscopy
Type A & B, with viscosities of 150 and 1250 cSt (centistokes),
respectively, meet these requirements. The greater the gap between
cover glass and objective, or condenser and slide, the more desirable
high viscosity becomes. Type A, with 150 cSt, reduces any tendency to trap air bubbles, thus avoiding image degradation.
Type B, with 1250 cSt, is thick enough for viewing multiple slides with one application, thus saving time during batch
processing. Cargille Immersion Oils A, B, NVH and 300 can be blended for intermediate viscosities.
For Inverted, Inclined, Projection and Long Focus Instruments.
Higher viscosities are generally required. The new Type NVH meets
this requirement, having a viscosity of 21,000 cSt.
Automated Hematology Systems
Type 300 has been specifically designed to meet results for optimum optical imaging and mechanical processing.
For Fluorescence Microscopy
Extremely low fluorescence is achieved by type LDF, the preferred immersion oil for this application. For
non-critical fluorescence microscopy applications, types A and B are sufficiently low fluorescing.
| Immersion Oil |
Type
A |
Type
B |
Type
NVH |
Type
300 |
Type LDF |
Refractive Index
F Line (486.1nm)
e Line (546.1nm)
D Line (589.3nm)
C Line (656.3nm) |
@23°C
1.5239
1.5180
1.5150
1.5115 |
@23°C
1.5236
1.5180
1.5150
1.5116 |
@23°C
1.5230
1.5178
1.5118
1.5118 |
@23°C
1.5238
1.5180
1.5150
1.5115 |
@23°C
1.5232
1.5179
1.5150
1.5116 |
Dispersion: nF-nc:
Abbe VD:
Abbe Ve:
|
0.0124
41.6
41.2 |
0.0120
42.9
42.6 |
0.0112
46.0
45.7 |
0.0123
41.8
41.5 |
0.0117
44.2
44 |
Temperature
Coefficient:
(15-35°C)-dnD/dt |
-0.00033 |
-0.00031 |
-0.00034 |
-0.00033 |
-0.00039 |
Stability:
(change in nd25°C
after 24 hrs.@60°C)
(@100°C) |
0
0 |
0
0 |
0
0 |
0
0 |
0
+0.0002 |
Fluorescence: (1)
Short wave:
(Ultraviolet)
Long Wave: |
low
low
|
low
low
|
low
low
|
low
low
|
very, very low
very, very low |
| Color (Gardner): |
1 |
1 |
1 |
1 |
<1 |
Viscosity:
cSt±1-%
@23°C:
(centistokes): |
150
low |
1250
high |
21,000
very high |
300
medium |
330
medium |
Density:
@23°C gm/cc:
US lb./gal: |
.923
7.70 |
.923
7.70 |
.919
7.67 |
.923
7.73 |
1.225
10.22 |
| Cloud Point: |
-13°C |
-13°C |
-13°C |
<-13°C |
-6°C |
Flash Point:
(Cleveland Open Cup) |
325°F
163°C |
325°F
163°C |
325°F
163°C |
325°F
163°C |
390°F
200°C |
Neutralization No:
(mgKOH/gm) |
0.01 max. |
0.01 max. |
0.01 max. |
0.01 max. |
0.15 max. |
Why do Cargille Immersion Oils Meet the DIN 58 884?
(Deutsches Institut Für Normung e.V.)
Refractive index, dispersion and temperature standardizations
are the key specifications for optical clarity and high resolution.
(Viscosity and other physical properties are directed toward
specific applications and convenience of the user.)
Immersion oil specifications must meet the design requirements
of old instruments and become the design criteria for new instruments.
Without these specifications, it would be much harder for the
microscopist to find the proper oil for his or her particular
instruments' requirements and optical imagery would suffer.
The specifications adopted by DIN resulted from their study of
instrument requirements of many manufacturers. The proposed specifications
were submitted for review, with Cargille Laboratories serving
as liaison for the American manufacturers.
The present trend toward tighter specification for optical properties
is under a program of the International Standards Organization
(ISO).
Unique Applications - Special Properties
Because of widespread availability, consistent quality, and close
specifications, Cargille Oils are used as:
- A temporary mounting media
- Fluid mounts (permitting specimen rotation)
- Calibration liquids
- Optical coupling fluids
- Combination protective coating and examination fluid
- Transparency medium for translucent materials
- Filter clearing agent (as recommended by Millipore Corp.)
Important Comments
Cargille Immersion Oils are supplied as a microscopy accessory
by American Optical and Bausch & Lomb, and accepted for use
by all foreign microscope manufacturers. Cargille Immersion Oils
are synthetic hydrocarbons, natural petroleum derivatives that
contain no solvents and no PCB's. These oils are stable, non-drying,
odorless, safe for lens and handling, and easily removable. MSDS
sheets are available.
Ordering Information
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Cargille Immersion Oil, Type A, low visc, 120ml (4 oz.) | | |
|
| U MSDS Low viscosity (150cs), low fluorescence, PCB-free. |
| Cargille Immersion Oil, Type B, high visc, 120ml (4 oz.) | | |
|
| U MSDS High viscosity (1250cs), low fluorescence, PCB-free. |
| Cargille Immersion Oil, Type NVH, very high viscosity, 120ml (4 oz.) | | |
|
| U MSDS Very high viscosity (21000cs), low fluorescence, PCB-free. |
| Cargille Immersion Oil, Type LDF, 30ml (1 oz.) | | |
|
| U MSDS Very high viscosity (21000cs), low fluorescence, PCB-free. |
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Cargille Immersion Oil, 5-Pack, 5 x 7.4cc (1/4 oz.), 1ea. Type 300, 1ea. Type NVH, 2ea. Type A, 1ea. Type B | | |
|
Cargille Meltmount™ for Microscopy and Optical Coupling
The Cargille Meltmount™ series of mounting media
are specially formulated optical-quality thermoplastics for use in
microscope slide mounting and in other optical coupling applications
- Cargille Meltmounts™ Are:
- Instant - they are thermal mountants and require "no
oven time"
- 100% usable - contain no solvents
- Less Expensive per slide
- Reversible - thermally, for particle retrieval or
re-orientation
- Soluble - in toluene if needed for special techniques or
clean-up
- Fluid - at 65°C - a temperature chosen because it makes
a permanent mount and protects the majority of specimens
from thermal changes.
- PCB-FREE
- Direct replacements for "older style" media
|
Old Media |
Meltmounts™ Equivalent |
| Canada Balsam |
Meltmount™
1.539 |
|
Aroclor 5442 |
Meltmount™
1.662 |
| Naphrax |
Meltmount™
1.704 |
Notes on use:
- Meltmount is a Thermal Plastic Material. This means its
viscosity is dependent on temperature (inversely dependent).
As the temperature increases the viscosity decreases. There
is no sharp melting point. Being thermal plastic, it is
capable of "cold flow". This means the Meltmount, the
specimen, the slide, and the cover slip can all move
independently of each other given a mix of time,
temperature, and lateral pressure or gravity.
- Storage of prepared slide: treat them as the valuable
items they are. Store:
- Flat, cover slip on top
- In the dark
- Away from dust and fumes
- At 60° to 85°F. Meltmount is meant to be thermally
reversible, don't allow this to happen inadvertently by
storing or transporting prepared slide above 85°F.
QUICK-STICK™
Cargille Meltmount™
is now available in a convenient stick form called Quick-Stick™.
It can be used to make permanent microscope slide mounts
quickly.
Quick-Stick™ can be
applied to a slide on a hotplate. As soon as the specimen and
cover glass are positioned and the slide is cooled, you have a
permanent prepared slide that can be reversed by reheating, if
you should wish to retrieve a particular particle.
Meltmount™
Price List and Description
Meltmount™ 1.539
Meltmount™ 1.539 has a refractive index (nD @ 25°C) of 1.539 and
an Abbe V dispersion of 45 making it optically similar to Canada
Balsam and, therefore, ideal for mounting many biological
specimens but without the long drying time of Canada Balsam.
MSDS
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Meltmount™ 1.539 | | |
|
| Meltmount™ 1.539 Quick-Stick™ | | |
|
Meltmount™ for Chrysotile Asbestos
Meltmount™ This product has dispersion characteristics making it appropriate for
mounting chrysotile asbestos.
MSDS
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Meltmount™ for Chrysotile | | |
|
Meltmount™ 1.582
Meltmount™ 1.582 has a refractive index (ND @ 25°C) of 1.582 and
an Abbe V dispersion of 33. Its optical clarity makes it the
preferred choice for minimum visible absorption.
MSDS
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Meltmount™ 1.582 | | |
|
| Meltmount™ 1.582 Quick-Stick™ | | |
|
Meltmount™ 1.605
Meltmount™ 1.605 has a refractive index (ND @ 25°C) of 1.605 and
an Abbe V dispersion of 30, making it appropriate for mounting
asbestiform Tremolite, Anthophyllite, and Actinolite.
MSDS
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Meltmount™ 1.605 | | |
|
| Meltmount™ 1.605 Quick-Stick™ | | |
|
Meltmount™ 1.662
Meltmount™ 1.662 has a refractive index (ND @ 25°C) of 1.662 and
an Abbe V dispersion of 26, optically similar to Aroclor 5442,
but is PCB-free.
MSDS
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Meltmount™ 1.662 | | |
|
| Meltmount™ 1.662 Quick-Stick™ | | |
|
Meltmount™ 1.680
Meltmount™ 1.680 has a refractive index (ND @ 25°C) of 1.680 and an Abbe V dispersion of 25,
making it appropriate for mounting Amosite and Crocidolite asbestos.
MSDS
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Meltmount™ 1.680 | | |
|
| Meltmount™ 1.680 Quick-Stick™ | | |
|
Meltmount™ 1.704
Meltmount™ 1.704 has a refractive index (ND @ 25°C) of 1.704,
similar to naphrax, and an Abbe V dispersion of 24.
MSDS
| Prod # | Description | Unit | Price | Order / Quote |
|---|
| Meltmount™ 1.704 | | |
|
| Meltmount™ 1.704 Quick-Stick™ | | |
|
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Page Last Updated: July 11, 2011
