Chemicals for Electron and Light Microscopy

in alphabetical order

Prod #	Description	Unit	Price	Order / Quote
18612	Acetonitrile, 450ml	each	$56.00	Qty:

F, T - MSDS

CH3CN; [75-05-08]; FW 41.05

Suggested substitute for Propylene Oxide.It is reported as noncarcinogenic, less toxic and not as flammable. High purity; miscible with water, alcohols, acetone and epoxy resins15. 15) Tarnowski BI, Schonbaum GR: Acetonitrile: substitute for propylene oxide in tissue processing for transmission electron microscopy. Proc 42nd Ann Meeting Elec Mic Soc Amer, p 38, 1984 Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR: Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: Comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Micro Res & Tech, 21, pp 39­50, 1992 Also used as a dehydrating agent for cells prepared for SEM: Holshek JG, Akins RE: Acetonitrile is better than ethanol as a dehydrating agent for cells prepared for SEM. Proc Mic Soc of Amer, 52nd Annual Mtg 1994, San Francisco Press, ed GW Bailey and AJ Garrett-Reed, pp 324-325, 1994

PELCO® Acrylic Embedding Resin Kit

Prod #	Description	Unit	Price	Order / Quote
18190	PELCO™ Acrylic Embedding Resin Kit, 500ml	each	$148.50	Qty:

F, I, T - PELCO® Acrylic Embedding Resin, 500ml MSDS O, I - Benzoyl Peroxide (~90.0%), 6.5g, (18190-B) MSDS Replaces Unicryl

A unique acrylic resin modeled after the old UNICRYL™ acrylic resin. The unique advantage to this resin is that the catalyst is supplied separate from the resin components. Shelf life is improved and heat induced changes during shipping are eliminated. This resin is ideally suited for LM and EM applications (immunocytochemistry, ultrastructural preservation, in situ hybridization with good cutting properties for either thick or thin sectioning). Unique cutting properties have been attributed to this resin formulation. Proteins, nucleic acids and macromolecules are revealed at the surface during sectioning. It is a hydrophilic resin which exhibits unique staining properties a the light level for a wide range of stains (i.e. hemotoxylin, silver methenamine, light green, eosin, safranin, Toluidine blue). The resin contains styrene monomer, monomeric (meth)- acrylate esters with benzoyl peroxide supplied as the initiator. Mixing of the initiator is required prior to use. The resin is best stored at -20°C after the addition of the initiator but the resin will remain liquid down to -50°C. It is a pale yellow liquid with a pungent odor that can be cured with either heat or UV radiation. Best results are attained when the resin is microwave cured. The resin should be capped during polymerization due to evaporation. Use proper gloves, fume hood and eye protection.

Araldite 502

Prod #	Description	Unit	Price	Order / Quote
18060	Araldite 502, 450g	each	$17.50	Qty:

I, T - MSDS

Detailed description under Araldite 502 Kit.

Background Buster

Prod #	Description	Unit	Price	Order / Quote
18722-50	Background Buster, 50ml	each	$225.00	Qty:

Detailed description under Background Buster.

Basic Acid Fuchsin

Prod #	Description	Unit	Price	Order / Quote
18703	Basic Acid Fuchsin, Certified, 25g	each	$74.90	Qty:

I - MSDS

C20H19N3Cl; [632-99-5]; FW 337.86

(Rosaniline Hypochloride; Magenta; Basic Violet 14) Berkowitz et al., (1968). Selective staining of nervous tissue for light microscopy following preparation for electron microscopy. J. Histochem. Cytochem. 16:808. Sato, T., and Shamoto, M., (1973). A simple rapid polychrome stain for epoxy embedded tissue. Stain Technol. 48:222.

Benzoin Methyl Ether

Prod #	Description	Unit	Price	Order / Quote
18186	Benzoin Methyl Ether, 10g	each	$40.65	Qty:

T, I - MSDS

[3524-62-7]; MW 226.3

UV Catalyst for Prod. No. 18183 LR Gold; use at 0.5% w/v, ~365nm.

Benzyldimethylamine

Prod #	Description	Unit	Price	Order / Quote
18241	Benzyldimethylamine, 25g	each	$5.75	Qty:

C, T, I, F - MSDS

C6H5CH2N(CH3)2; [103-83-3]; FW 135.21

(BDMA) N,N-Dimethylbenzylamine Catalyst for epoxy resins. Has lower viscosity compared to DMP-30. (Also stores longer.)

Cacodylic Acid, Sodium Salt, trihydrate

Prod #	Description	Unit	Price	Order / Quote
18851	Cacodylic Acid, Sodium Salt, trihydrate, 100g	each	$107.60	Qty:

T (highly) - MSDS

(CH3)2As(O)ONa-3H2O; [124-65-2]; FW 214.02;UN1688

Arsenic compound. Highly toxic. Hygroscopic. Sabatini DD, Miller F, Barrnett RJ: Cytochemistry and electron microscopy. The preservation of cellular structure and enzymatic activity by aldehyde fixation. J Cell Biol, 17, 19­58, 1963

Citifluor Mountant Media
Complete discription and micrograph examples

Prod #	Description	Unit	Price	Order / Quote
Citifluor Antifadent (AF) Solutions
19470	AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 25ml	each	$85.75	Qty:
19470-1	AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 100ml	each	198.00	Qty:
I - MSDS (pdf 109K)
19472	AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 25ml	each	92.90	Qty:
19472-1	AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 100ml	each	198.00	Qty:
I - MSDS (pdf 109K) Glycerol solution, additives.
19474	AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 25ml	each	86.35	Qty:
19474-1	AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 100ml	each	198.00	Qty:
I - MSDS(pdf 108K)
19476-A	Citifluor non-fluorescent Immersion Oil, AFB7, 10ml	each	74.50	Qty:
I - MSDS(pdf 107K)
19476	AF-4, Glycerol solution containing n-propyl gallate for use with labelled tissue sections and dead cells, 10ml	each	86.35	Qty:
19490	AF-100, Phosphate-buffered saline solution containing antifadent specially prepared for use with UVM23 solution where fluorochrome fading is a problem, 5ml	each	8.45	Qty:
I - MSDS (pdf 109K)
19491	AF-200, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used with other fluorochromes such as fluorescein, Alexa dyes and Hoescht dye, 10ml	each	65.80	Qty:
I - MSDS (pdf 108K)
19492	AF-300, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used where fluorescein is the staining material. The solution can also be used with other fluorochromes such as rhodamines, etc., 10ml	each	65.80	Qty:
I - MSDS (pdf 109K)
19493	CFM1, Glycerol-PBS ref. index similar to glass, 10ml	each	45.50	Qty:
I - MSDS (pdf 109K)
19494	CFM1, Glycerol-PBS w/AF, ref. index similar to glass, 10ml	each	52.70	Qty:
I - MSDS (pdf 135K)
19495	CFM2, Glycerol-TRIS buffer, ref. index similar to glass, 10ml	each	45.50	Qty:
I - MSDS (pdf 110K)
19496	CFM3, Glycerol-Phosphate & AF, ref. index similar to glass, 10ml	each	52.70	Qty:
I - MSDS (pdf 109K)
UV Mount Media (UVM)
19497	UVM1, Methacrylate based solution, UV curable, gives clear film, 10ml	each	45.50	Qty:
I - MSDS (pdf 69K)
19498	UVM2, Methacrylate based solution, contains anti-bleaching agent, 10ml	each	45.50	Qty:
I - MSDS (pdf 90K)
19499	UVM3, High refractive index methacrylate-based solution designed to be used with AF-100 where fluorochrome fading is a problem, 10ml	each	57.75	Qty:
I - MSDS (pdf 68K)
Solid Mountants
19469	Citifluor Solid Mountant Kit is comprised of PVA solution, 25ml and Citifluor antifadent in buffer, 5ml. The components can be mixed as required, applied to the specimen and allowed to dry to give a clear film, 30ml	each	95.00	Qty:
C - MSDS for Citifluor AF100 Antifadent Solution, 25ml (pdf 183K)
U - MSDS for Citifluor CFPVOH Solution, 5ml (pdf 176K)
Contains anti-fading solution. Suitable for preventing fading of dyes such as DAPI which have the tendency to disperse in glycerol based mounting solutions. Colloidal Adhesives: See "Adhesives"

Cresyl (Fast) Violet, Certified

Prod #	Description	Unit	Price	Order / Quote
18709	Cresyl (Fast) Violet, Certified, C.I. #51180, 5g	each	$88.60	Qty:

U - MSDS

C18H15N3O3 [10510-54-0] FW 312.34

(Cresyl Violet Acetate) Solubility: 0.13% water

Nguyen KB and Pender MP, 1989. Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining. Stain Technology 64:4, pp. 163-167.

DAPI, fluorescent Dye

Prod #	Description	Unit	Price	Order / Quote
18706	DAPI, fluorescent Dye (powder), 10mg	each	$141.20	Qty:

I - MSDS

C16H15N5•2HCl; [28718-90-3]; FW 350.3

Cell permeable fluorescent minor grove-binding probe for DNA (powder). 4',6-Diamidino-2-phenylindole Dihydrochloride:Hydrate

Deionized Water

Prod #	Description	Unit	Price	Order / Quote
18708	Deionized Water, 1 gallon	each	$10.70	Qty:

For general laboratory use

Diglycidyl Ether of Poly (propylene glycol) (DER 736)

Prod #	Description	Unit	Price	Order / Quote
18310	Diglycidyl Ether of Poly (propylene glycol) (DER 736), 250g	each	$18.70	Qty:

I - MSDS

[41638-13-5]; Specific gravity 1.14.; Epoxy flexibilizer; Viscosity 33.6; Cp @ 25°C.

2-Dimethylaminoethanol (DMAE)

Prod #	Description	Unit	Price	Order / Quote
18315	2-Dimethylaminoethanol (DMAE), 25g	each	$8.20	Qty:

C, L - MSDS

C4H11NO; [108-01-0]; FW 89.14

2,4,6-Tri[Dimethylaminomethyl]phenol) (DMP-30)

Prod #	Description	Unit	Price	Order / Quote
18042	(2,4,6-Tri[Dimethylaminomethyl]phenol) (DMP-30), 25g	each	$5.90	Qty:

I - MSDS

[90-70-2]; FW 265.0 Tertiary amine accelerator. Inactivated by water. Store in dark in vacuum desiccator. DMP dehydration for EM: Rapid Chemical Dehydration of Samples for Electron Microscopic Examinations. L.L. Muller and T.J. Jacks. The Journal of Histochemistry and Cytochemistry. Vol. 23, No. 2, pp.107-110, 1975. 2,2-Dimethoxypropane, a rapid dehydrating agent for scanning electron microscopy. W.S.Johnson, G.R. Hooper, B.F. Holdaway, and H.P. Rasumssen. Micron, 1976, Vol. 7:305-306. Rapid Chemical Dehydration of Biologic Samples for Scanning Electron Microscopy using 2,2-Dimethoxypropane. Morton D. Maser and John J. Trimble, III. The Journal of Histochemistry and Cytochemistry. Vol. 25, No. 4, pp. 247-251. 1977. Optimization and Investigation of the Use of 2,2-Dimethoxypropane as a Dehydration Agent for Plant Tissues in Transmission Electron Microscopy. J.R. Thorpe and Diana M.R. Harvey. Journal of Ultrastruture Research, 68, 186-194 (1979).

Dodecenyl Succinic Anhydride (DDSA)

Prod #	Description	Unit	Price	Order / Quote
18022	Dodecenyl Succinic Anhydride (DDSA), 450g	each	$21.90	Qty:

I - MSDS

[26544-38-7]; FW 266.38 Double-distilled. Moisture sensitive. Hardener for epoxy resins. Viscosity 290-295cP, density 1.005g/ml.

Eponate 12® Resin

Prod #	Description	Unit	Price	Order / Quote
18005	Eponate 12™ Resin, 450g	each	$44.25	Qty:

I, Cg - MSDS

[25038-04-4] Substitute for "Epon®" recipes and our Medcast (tm). Excellent results with traditional EM specimen block preparations. WPE 140-150. Viscosity @ 25°'C 130-170cP. Specific Gravity 1.24. Moisture sensitive.

1,2-epoxypropane (see Propylene Oxide) MSDS

ERL 4221 Epoxide Resin

Prod #	Description	Unit	Price	Order / Quote
18306-4221	ERL 4221 Epoxide Resin, 225ml	each	$23.70	Qty:

Cg - MSDS

C14H20O4; [2386-87-0; 2611-00-9]; FW 1.173

A Cycloaliphatic Epoxide Resin specially formulated to take the place of ERL 4206 which is no longer available.

Ethyl Alcohol, Anhydrous

Prod #	Description	Unit	Price	Order / Quote
19206	Ethyl Alcohol, Anhydrous, 1 Liter, A.C.S., glass distilled	each	$76.70	Qty:

F, T - MSDS

C2H5OH; [64-17-5]; FW 46.07

Ethyl Alcohol

Prod #	Description	Unit	Price	Order / Quote
19207	Ethyl Alcohol, 200ml, A.C.S., glass distilled	each	$8.90	Qty:

MSDS

Formaldehyde, 16%

Prod #	Description	Unit	Price	Order / Quote
18505	Formaldehyde, 16%, 10 x 10ml	each	$26.40	Qty:

Cg, T (highly) - MSDS PELCO® Technical Notes, Formaldehyde, Formalin (pdf)

HCHO; [50-00-0]; FW 30.03 EM grade. Paraformaldehyde solution (methanol free). Pre-scored amber ampules sealed under inert gas.

Prod #	Description	Unit	Price	Order / Quote
18505-100	Formaldehyde, 16%, 100ml	each	$24.60	Qty:

Cg, T (highly) - MSDS PELCO® Technical Notes, Formaldehyde, Formalin (pdf)

HCHO; [50-00-0]; FW 30.03 EM grade. Paraformaldehyde solution (methanol free). 100ml.

Formaldehyde, 37%

Prod #	Description	Unit	Price	Order / Quote
18508	Formaldehyde, 37%, microfiltered (Formalin), 10 x 10ml	each	$32.90	Qty:

Cg, T (highly) - MSDS PELCO® Technical Notes, Formaldehyde, Formalin (pdf) Histological Fixative (35 - 38% Formaldehyde, 10 - 15% Methanol)

Formalin, 10% Neutral Buffered in Pre-Fill Containers

This traditional fixative is buffered to a neutral pH. It is used in histological applications for fixing and storing tissue specimens. This line of all plastic containers is designed specifically for the collection and transportation of various sizes of tissue samples. Each container is half filled with 10% neutral buffered formalin. The sizes offered are 20ml and 120ml. They are packaged in the proprietary Sure-Lok system. These conatiners are 95 kPa compliant.

Prod #	Description	Unit	Price	Order / Quote
18511	NB Pre-Fill Formalin 20ml Container	case/128	$49.20	Qty:
18512	NB Pre-Fill Formalin 20ml Container	case/780	277.60	Qty:
18513	NB Pre-Fill Formalin 40ml Container	case/50	20.55	Qty:
18514	NB Pre-Fill Formalin 60ml Container	case/50	21.10	Qty:
18515	NB Pre-Fill Formalin 90ml Container	case/50	28.10	Qty:

Cg, T (highly) - MSDS PELCO® Technical Notes, Formaldehyde, Formalin (pdf)

Histological Fixative. Phosphate Buffer. pH 6.9 - 7.1 at 25°C. Note: ACS Reagent Grade Formalin (35 - 38% Formaldehyde, 10 - 15% Methanol) larger quantities available on request.

Formalin, 10% Neutral Buffered

Prod #	Description	Unit	Price	Order / Quote
18510	Formalin, 10% Neutral Buffered, 450ml	each	$14.25	Qty:

Cg, T (highly) - MSDS PELCO® Technical Notes, Formaldehyde, Formalin (pdf)

Histological Fixative. Phosphate Buffer. pH 6.9 - 7.1 at 25°C. Note: ACS Reagent Grade Formalin (35 - 38% Formaldehyde, 10 - 15% Methanol) larger quantities available on request.

Formvar® see Vinylec® (Formvar® substitute)

Gelatin, Purified

Prod #	Description	Unit	Price	Order / Quote
19225	Gelatin, Purified, 100g	each	$49.40	Qty:

Prod #	Description	Unit	Price	Order / Quote
155-9	GridStick™" Adhesive, 5ml	each	$12.50	Qty:

F, I, T - MSDS

Used to adhere SynapTek™ grids to the GridStick™.

Hexamethyldisilazane (HMDS)

Prod #	Description	Unit	Price	Order / Quote
18605	Hexamethyldisilazane (HMDS), 250ml	each	$78.00	Qty:

C, F, I, T - MSDS

(CH3)3SiNHSi(CH3)3; [999-97-3]; FW 161.40 Drying tissues for SEM. Stain Tech, 58(6), 347, 1983. Possible substitute for Peldri ll®

Hematoxylin, Certified

Prod #	Description	Unit	Price	Order / Quote
18721	Hematoxylin, Certified, C.I. #75290, 10g	each	$31.50	Qty:

I - MSDS

C16H14O6; [517-28-2]; FW 302.29 (Natural Black l, Hydroxybrasilin) Solubility: 10.0% Water; 10.0% Alcohol; 9.5% Cellosolve; 10% Glycol; 0.0% Xylene. A certified biological stain used in histology and cytology and plastic embedded tissues. Chen, Chang, S., J Arch. of Path., 93, 334 (1972).

Prod #	Description	Unit	Price	Order / Quote
18020	JB-4® Embedding Kit	each	$304.60	Qty:

F, I, T

Prod #	Description	Unit	Price	Order / Quote
18040	JB-4® Plus Embedding Kit	each	$273.35	Qty:

F, I, T

Prod #	Description	Unit	Price	Order / Quote
18027	Liquid Release Agent, 100ml	each	$4.90	Qty:

U

A hydrosoluble mold-release agent for use in flat embedding of micro-organisms, and as a replacement for the PTFE coating of glass slides to produce a smoother, even surface. The reference below describes the selection and precisely oriented sectioning of single cells by light microscopy, prior to examination. Reymond OL, Picket-Heaps JD, 1983. J Microscopy, 130, p. 179.

LR Gold Resin

Prod #	Description	Unit	Price	Order / Quote
18183	LR Gold Resin, 500ml	each	$162.50	Qty:

I - MSDS

Resin (acrylic) only, no catalyst included. Requires Catalyst Prod. No. 18186: UV, benzoin methyl ether MSDS (~365nm) (0.5% w/v); "blue light", benzil MSDS (~460nm) (0.1% w/v); benzoyl peroxide MSDS(1% w/v), cold-cure. Accurate measurements of catalyst are very important. LR Gold can be used at low temperatures (-25°C) and has proven very useful for immunogold procedures. Very low viscosity: 8cP. Applied to both LM (enzyme histochemistry) and EM (immunochemistry) techniques. Technical Notes (PDF 1,816KB)

Benzoin Methyl Ether

Prod #	Description	Unit	Price	Order / Quote
18186	Benzoin Methyl Ether, 10g	each	$40.65	Qty:

T, I - MSDS

[3524-62-7]; MW 226.3

UV Catalyst for Prod. No. 18183 LR Gold; use at 0.5% w/v, ~365nm.

Prod #	Description	Unit	Price	Order / Quote
18185	Accelerator for LR white, 10ml	each	$12.90	Qty:

T - MSDS

Lead Citrate, Trihydrate

Prod #	Description	Unit	Price	Order / Quote
19312	Lead Citrate, Trihydrate, 10g	each	$17.90	Qty:

I, T - MSDS

Pb3(C6H5O7); 3H2O; [512-26-5]; FW 1053.82

Prod #	Description	Unit	Price	Order / Quote
19314	Lead Citrate, Trihydrate, 50g	each	$48.30	Qty:

I, T - MSDS

Lowicryl HM-20 MonoStep (non polar)

Prod #	Description	Unit	Price	Order / Quote
18174	Lowicryl HM-20 MonoStep (non polar), 225g Pre-mixed, ready to use embedding media	each	$222.45	Qty:

I, O, T - MSDS

Methylamine Tungstate

Prod #	Description	Unit	Price	Order / Quote
18353	Methylamine Tungstate, 1g	each	$75.85	Qty:

U - MSDS

An excellent negative stain. Unlike phosphotungstic Acid, it does not damage virus particles and it is consequently valuable for staining delicate viruses. Faberge AC, and Oliver RM, 1974. Methylamine Tungstate, a New Negative Stain. J. Microscopy 20, pp. 241-246.

NMA

Prod #	Description	Unit	Price	Order / Quote
18032	NMA, 450g	each	$21.90	Qty:

C, I - MSDS

C10H10O3; [25134-21-8]; FW 178.19 Methyl-5-norbornene-2,3-dicarboxylic anhydride. Moisture sensitive. Viscosity 225-275cp, density 1.24g/ml. Hardener for epoxy resins.

Nonenyl Succinic Anhydride, (NSA)

Prod #	Description	Unit	Price	Order / Quote
18301	Nonenyl Succinic Anhydride, (NSA), 450g	each	$29.80	Qty:

I - MSDS

[28928-97-4] Double-distilled. Epoxy resin hardener.

Prod #	Description	Unit	Price	Order / Quote
19483	OTE EM Stain, 10g	each	$125.00	Qty:

I -

Substitue for Uranyl Acetate in staining of ultrathin sections. Ref: Sato, S., Sasaki, Y., Adachi, A., Dai, W., Liu, S-L. (2003) Use of oolong tea extract (OTE) for clastin staining and enhancement in ultrathin sections. Med. Electron Microsc. 36, 179-182. Sato, S., Sasaki, Y., Adachi, A., Dai, W., Liu, S-L. (2007) Oolong tea extract (OTE) as a substitute for uranyl acetate in staining of ultrathin sections. Journal of Microscopy, Vol 229, Pt.1 2008, pp17-20

Paraformaldehyde, EM Grade

Prod #	Description	Unit	Price	Order / Quote
18501	Paraformaldehyde, EM Grade, Prill Purified, 1kg	each	$22.90	Qty:

T, F, I, Cg - MSDS

(HO(CH2O)nH; [30525-89-4]; FW~600 Fixative. Polyoxymethylene. White free-flowing solid prill with strong, pungent formaldehyde odor. Avoid strong oxidizing agents, temperatures above 38°C. Karnovsky MJ: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol, 27, 137A, 1965 Note: Karnovsky's 1965 fixative contains 5% glutaraldehyde and 4% formaldehyde in a sodium cacodylate buffer and is very hypotonic (p. 5:1.17, alternative solution strength and formulation suggested)5. (Reference 5, p. 64) Robertson JD: The occurence of a subunit pattern in the unit membranes of club endings in Maunther cell synapses in gold fish brain. J Cell Bio, 19, 201, 1963

PELCO® Pro CA44 Tissue Adhesive PELCO® Pro CA44 is an instant tissue adhesive based on cyanoacrylate. Developed to glue tissue on specimen holders used in vibrating microtomes, such Microslicer and Vibratome. Although the tissue adhesive is instable in boiling water, it is recommended to use #19920 PELCO® Pro Cyanoacrylate Debonder (below) to remove or clean cured cyanoacrylate. Supplied in 20ml bottle with dispenser tip, closed with ptfe coated steel pin for optimum seal. Compatible to Loctite 404 tissue adhesive.

Prod #	Description	Unit	Price	Order / Quote
19402	Phosphotungstic Acid, Reagent (PTA), 25g	each	$37.90	Qty:

I - MSDS

PELCO® Pro Cyanoacrylate Debonder The PELCO® Pro Cyanoacrylate Debonder destabilizes and debonds cured cyanoacrylates. Useful for releasing specimens from holders, cleaning cyanoacrylates from surfaces and removing cured cyanoacylates from fingers. Works on all brands of cyanoacrylate glue (Loctite, Henkel, Permabond, 3M™, PELCO®, Aron Alpha, etc.). The debonder is also a solvent, please check before using on plastic surfaces / samples and avoid repeated skin contact. Supplied in 20ml bottle with dispenser tip.

Prod #	Description	Unit	Price	Order / Quote
19920	PELCO® Pro Cyanoacrylate Debonder, 20ml	each	$5.69	Qty:

F, Cg, I, T - MSDS

Phosphotungstic Acid, Reagent (PTA)

Prod #	Description	Unit	Price	Order / Quote
19402	Phosphotungstic Acid, Reagent (PTA), 25g	each	$37.90	Qty:

Prod #	Description	Unit	Price	Order / Quote
19244	Pioloform Powder, 10g	each	$6.90	Qty:

I - MSDS

Substrate component. Water-insoluble. Good thermal and mechanical stability. Because of small mass thickness, electron scattering within the film is low. 0.3-1.5% solution in chloroform, filtered through filter paper.

Prod #	Description	Unit	Price	Order / Quote
18601	Propylene Oxide, EM Grade, 450ml	each	$24.20	Qty:

Cg, F, I Special Caution - MSDS

CH3CHCH2O [75-56-9] FW 58.08 1,2-epoxypropane. Must use under hood, and with all caution. Extremely low vapor pressure. "Since epoxy resins are soluble in ethanol and acetone, dehydration is commonly carried out with one of these solvents. Because epoxy resins are more readily soluble in propylene oxide...this solvent is frequently used immediately prior to infiltration with the resin. ...most resins are not readily miscible with ethanol". Propylene Oxide does not separate from the epoxy resin during curing, thus small amounts left in the resin do no harm. Luft introduced Propylene Oxide into epoxy mixtures with his landmark paper and helped bring about improved embedding cures, better ultrastructural research results with blocks that would more reliably section and give better performance under the EM beam by reducing viscosity and increasing penetration.

Ruthenium Red

Prod #	Description	Unit	Price	Order / Quote
19421	Ruthenium Red, 1g	each	$49.80	Qty:

I - MSDS

[(NH3)5 RuoRu(NH3) 4 ORu(NH3)5]Cl6 · 4H2O [11103-72-3]; FW 786.36

Sodium Hydroxide, Pellets, Reagent, ACS

Prod #	Description	Unit	Price	Order / Quote
19539	Sodium Hydroxide, Pellets, Reagent, ACS, 500g	each	$36.20	Qty:

C, T - MSDS

NaOH, [1310-73-2]; FW 40

Sodium Hydroxide, 2N Solution, carbonate-free

Prod #	Description	Unit	Price	Order / Quote
19540	Sodium Hydroxide, 2N Solution, carbonate-free, 225ml	each	$15.60	Qty:

C, T - MSDS

NaOH; [1310-73-2]; FW 40 Normality 1.98­2.02, pH 8.6. Recommended: Bils RF: Electron Microscopy Laboratory Manual and Handbook, 2nd ed., 1992, p 154.

Sodium Phosphate, monobasic, anhydrous, Reagent, ACS

Prod #	Description	Unit	Price	Order / Quote
19542	Sodium Phosphate, monobasic, anhydrous, Reagent, ACS, 1000g	each	$38.80	Qty:

I - MSDS

NaH2PO4 ; [7558-80-7]; FW 119.98 Hygroscopic.

Sodium Phosphate, dibasic, heptahydrate, Reagent, ACS

Prod #	Description	Unit	Price	Order / Quote
19545	Sodium Phosphate, dibasic, heptahydrate, Reagent, ACS, 450g	each	$35.85	Qty:

I - MSDS

Na2HPO4 · 7H2O; [7782-85-6]; FW 268.07

Sodium Sulfite, Reagent, ACS

Prod #	Description	Unit	Price	Order / Quote
19437	Sodium Sulfite, Reagent, ACS, 500g	each	$38.00	Qty:

I - MSDS

Na2SO3; [7757-83-7]; FW 126.04 Hygroscopic. Photographic developer.

"Spurr" Kit

Prod #	Description	Unit	Price	Order / Quote
18300	"Spurr" Kit	each	$91.50	Qty:

I

See "Kits" for complete description and hazards

Tissue-Tack Adhesive

Prod #	Description	Unit	Price	Order / Quote
19250	Tissue-Tack Adhesive, 100ml	each	$60.25	Qty:

I - MSDS

Permanent attachment of sections to glass slides during caustic or prolonged washes. For routine, immunofluorescent, immunoperoxidase staining.

Toluidine Blue O

Prod #	Description	Unit	Price	Order / Quote
19451	Toluidine Blue O, certified, 25g	each	$29.80	Qty:

I, T - MSDS

C15H16CLN3 S; [92-31-9] For epoxy sections: 0.5% toluidine blue in 0.1% sodium carbonate solution at pH 11.1 For methacrylate/acrylic sections: 0.05% toluidine blue in benzoate buffer pH 4.4 (benzoic acid 0.25g, sodium benzoate 0.29g, water 200ml). p 5:3.11, "Procedures in Electron Microscopy".

Uranyl Acetate

Prod #	Description	Unit	Price	Order / Quote
19481	Uranyl Acetate, 25g	each	$153.40	Qty:

T, R (depleted) - MSDS PELCO® Technical Notes, Uranium Salts for Staining, Uranyl Acetate (pdf)

UO2(CH3COO)2 - 2H2O; [6159-44-0]; FW 424.14 Positive or negative stain, EM. May cause adverse mutagenic or teratogenic effects.

Vinylec® (Formvar® substitute)

Prod #	Description	Unit	Price	Order / Quote
19223	Vinylec® - Formvar® substitute, 100g	each	$12.00	Qty:

I - MSDS soon to come

Vinylec® is a drop in substitute for Formvar® (polyvinyl formal). The original manufacturer (Monsanto) has sold parts of the business and the same product is now manufactured under the name Vinylec®. Vinylec® (Formvar® substitute) is and excellent and widely used material to make support films on TEM grids.