Home Page Abbreviated Contents Customer Login Quick Order / View Order
Contact Us Search Indexes Finish Order


Chemicals Index

Chemicals for Electron and Light Microscopy

in alphabetical order


Hazard Warning Guides Used in the Following Listings
C = Corrosive
Cg = Suspected Carcinogen
F = Flammable
I = Irritant
L = Lachrymator
O = Oxidizing
R = Radioactive
S = Sensitizer
T = Toxic
U = Unlisted as hazard; unknown hazard if any
An extra shipping charge is required by the Carriers when hazardous goods are transported.




Acetonitrile

Prod #DescriptionUnitPriceOrder / Quote
18612
Acetonitrile, 450ml
each
$56.00
Qty:
F, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
CH3CN; [75-05-08]; FW 41.05

Suggested substitute for Propylene Oxide. It is reported as noncarcinogenic, less toxic and not as flammable. High purity; miscible with water, alcohols, acetone and epoxy resins15.

15) Tarnowski BI, Schonbaum GR: Acetonitrile: substitute for propylene oxide in tissue processing for transmission electron microscopy. Proc 42nd Ann Meeting Elec Mic Soc Amer, p 38, 1984

Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR: Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: Comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Micro Res & Tech, 21, pp 39­50, 1992

Also used as a dehydrating agent for cells prepared for SEM: Holshek JG, Akins RE: Acetonitrile is better than ethanol as a dehydrating agent for cells prepared for SEM. Proc Mic Soc of Amer, 52nd Annual Mtg 1994, San Francisco Press, ed GW Bailey and AJ Garrett-Reed, pp 324-325, 1994






Araldite 502

Prod #DescriptionUnitPriceOrder / Quote
18060
Araldite 502, 450g
each
$19.85
Qty:
I, T - MSDS
Detailed description under Araldite 502 Kit.





Background Buster

Prod #DescriptionUnitPriceOrder / Quote
18722-50
Background Buster, 50ml
each
$289.50
Qty:
Detailed description under Background Buster.




Benzoin Methyl Ether

Prod #DescriptionUnitPriceOrder / Quote
18186
Benzoin Methyl Ether, 10g
each
$50.75
Qty:
T, I - MSDS
[3524-62-7]; MW 226.3
UV Catalyst for Prod. No. 18183 LR Gold; use at 0.5% w/v, ~365nm.





Benzyldimethylamine

Prod #DescriptionUnitPriceOrder / Quote
18241
Benzyldimethylamine, 25g
each
$8.00
Qty:
C, T, I, F - MSDS
C6H5CH2N(CH3)2; [103-83-3]; FW 135.21
(BDMA) N,N-Dimethylbenzylamine Catalyst for epoxy resins. Has lower viscosity compared to DMP-30. (Also stores longer.)




Cacodylic Acid, Sodium Salt, trihydrate

Prod #DescriptionUnitPriceOrder / Quote
18851
Cacodylic Acid, Sodium Salt, trihydrate, 100g
each
$107.60
Qty:
T (highly) - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
(CH3)2As(O)ONa-3H2O; [124-65-2]; FW 214.02;UN1688
Arsenic compound. Highly toxic. Hygroscopic.
Sabatini DD, Miller F, Barrnett RJ: Cytochemistry and electron microscopy. The preservation of cellular structure and enzymatic activity by aldehyde fixation. J Cell Biol, 17, 19­58, 1963




Citifluor Mountant Media

Complete description and micrograph examples

Prod #DescriptionUnitPriceOrder / Quote
Citifluor Antifadent (AF) Solutions
19470
AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 25ml
each
$76.50
Qty:
19470-1
AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 100ml
each
198.00
Qty:
I - MSDS (pdf 109K)
19472
AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 25ml
each
76.50
Qty:
19472-1
AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 100ml
each
198.00
Qty:
I - MSDS (pdf 109K)
Glycerol solution, additives.
19474
AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 25ml
each
76.50
Qty:
19474-1
AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 100ml
each
198.00
Qty:
I - MSDS(pdf 108K)
19476-A
Citifluor non-fluorescent Immersion Oil, AFB7, 10ml
each
68.50
Qty:
I - MSDS(pdf 107K)
19476
AF-4, Glycerol solution containing n-propyl gallate for use with labelled tissue sections and dead cells, 10ml
each
76.50
Qty:
19490
AF-100, Phosphate-buffered saline solution containing antifadent specially prepared for use with UVM23 solution where fluorochrome fading is a problem, 5ml
each
15.20
Qty:
I - MSDS (pdf 109K)
19491
AF-200, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used with other fluorochromes such as fluorescein, Alexa dyes and Hoescht dye, 10ml
each
68.50
Qty:
I - MSDS (pdf 108K)
19492
AF-300, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used where fluorescein is the staining material. The solution can also be used with other fluorochromes such as rhodamines, etc., 10ml
each
68.50
Qty:
I - MSDS (pdf 109K)
19493
CFM1, Glycerol-PBS ref. index similar to glass, 10ml
each
45.50
Qty:
I - MSDS (pdf 109K)
19494
CFM1, Glycerol-PBS w/AF, ref. index similar to glass, 10ml
each
52.70
Qty:
I - MSDS (pdf 135K)
19495
CFM2, Glycerol-TRIS buffer, ref. index similar to glass, 10ml
each
45.50
Qty:
I - MSDS (pdf 110K)
19496
CFM3, Glycerol-Phosphate & AF, ref. index similar to glass, 10ml
each
52.70
Qty:
I - MSDS (pdf 109K)
UV Mount Media (UVM)
19497
UVM1, Methacrylate based solution, UV curable, gives clear film, 10ml
each
45.50
Qty:
I - MSDS (pdf 69K)
19498
UVM2, Methacrylate based solution, contains anti-bleaching agent, 10ml
each
45.50
Qty:
I - MSDS (pdf 90K)
19499
UVM3, High refractive index methacrylate-based solution designed to be used with AF-100 where fluorochrome fading is a problem, 10ml
each
62.35
Qty:
I - MSDS (pdf 68K)
Solid Mountants
19469
Citifluor Solid Mountant Kit is comprised of PVA solution, 25ml and Citifluor antifadent in buffer, 5ml. The components can be mixed as required, applied to the specimen and allowed to dry to give a clear film, 30ml
each
95.00
Qty:
C - MSDS for Citifluor AF100 Antifadent Solution, 25ml (pdf 183K)
U - MSDS for Citifluor CFPVOH Solution, 5ml (pdf 176K)
Contains anti-fading solution. Suitable for preventing fading of dyes such as DAPI which have the tendency to disperse in glycerol based mounting solutions.
Colloidal Adhesives: See "Adhesives"





Cresyl (Fast) Violet, Certified

Prod #DescriptionUnitPriceOrder / Quote
18709
Cresyl (Fast) Violet, Certified, C.I. #51180, 5g
each
$88.60
Qty:
U - MSDS
C18H15N3O3 [10510-54-0] FW 312.34
(Cresyl Violet Acetate) Solubility: 0.13% water
Nguyen KB and Pender MP, 1989. Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining. Stain Technology 64:4, pp. 163-167.





DAPI, fluorescent Dye

Prod #DescriptionUnitPriceOrder / Quote
18706
DAPI, fluorescent Dye (powder), 10mg
each
$188.85
Qty:
I - MSDS
C16H15N5•2HCl; [28718-90-3]; FW 350.3
Cell permeable fluorescent minor grove-binding probe for DNA (powder).
4',6-Diamidino-2-phenylindole Dihydrochloride:Hydrate





Deionized Water

Prod #DescriptionUnitPriceOrder / Quote
18708
Deionized Water, 1 gallon
each
$10.70
Qty:
For general laboratory use





Diglycidyl Ether of Poly (propylene glycol) (DER 736)

Prod #DescriptionUnitPriceOrder / Quote
18310
Diglycidyl Ether of Poly (propylene glycol) (DER 736), 250g
each
$20.00
Qty:
I - MSDS
[41638-13-5]; Specific gravity 1.14.; Epoxy flexibilizer; Viscosity 33.6; Cp @ 25°C.





2-Dimethylaminoethanol (DMAE)

Prod #DescriptionUnitPriceOrder / Quote
18315
2-Dimethylaminoethanol (DMAE), 25g
each
$8.20
Qty:
C, L - MSDS
C4H11NO; [108-01-0]; FW 89.14





2,4,6-Tri[Dimethylaminomethyl]phenol) (DMP-30)

Prod #DescriptionUnitPriceOrder / Quote
18042
(2,4,6-Tri[Dimethylaminomethyl]phenol) (DMP-30), 25g
each
$5.90
Qty:
I - MSDS
[90-70-2]; FW 265.0
Tertiary amine accelerator.
Inactivated by water. Store in dark in vacuum desiccator.
DMP dehydration for EM:
Rapid Chemical Dehydration of Samples for Electron Microscopic Examinations. L.L. Muller and T.J. Jacks. The Journal of Histochemistry and Cytochemistry. Vol. 23, No. 2, pp.107-110, 1975.
2,2-Dimethoxypropane, a rapid dehydrating agent for scanning electron microscopy. W.S.Johnson, G.R. Hooper, B.F. Holdaway, and H.P. Rasumssen.
Micron, 1976, Vol. 7:305-306.
Rapid Chemical Dehydration of Biologic Samples for Scanning Electron Microscopy using 2,2-Dimethoxypropane. Morton D. Maser and John J. Trimble, III. The Journal of Histochemistry and Cytochemistry. Vol. 25, No. 4, pp. 247-251.
1977.
Optimization and Investigation of the Use of 2,2-Dimethoxypropane as a Dehydration Agent for Plant Tissues in Transmission Electron Microscopy. J.R. Thorpe and Diana M.R. Harvey.
Journal of Ultrastruture Research, 68, 186-194 (1979).





Dodecenyl Succinic Anhydride (DDSA)

Prod #DescriptionUnitPriceOrder / Quote
18022
Dodecenyl Succinic Anhydride (DDSA), 450g
each
$32.20
Qty:
I - MSDS
[26544-38-7]; FW 266.38
Double-distilled. Moisture sensitive. Hardener for epoxy resins. Viscosity 290-295cP, density 1.005g/ml.




Eponate 12® Resin

Prod #DescriptionUnitPriceOrder / Quote
18005
Eponate 12™ Resin, 450g
each
$46.50
Qty:
I, Cg - MSDS
[25038-04-4]
Substitute for "Epon®" recipes and our Medcast (tm). Excellent results with traditional EM specimen block preparations. WPE 140-150. Viscosity @ 25°'C 130-170cP. Specific Gravity 1.24. Moisture sensitive.

1,2-epoxypropane (see Propylene Oxide) MSDS




ERL 4221 Epoxide Resin

Prod #DescriptionUnitPriceOrder / Quote
18306-4221
ERL 4221 Epoxide Resin, 225ml
each
$25.00
Qty:
Cg - MSDS
C14H20O4; [2386-87-0; 2611-00-9]; FW 1.173
A Cycloaliphatic Epoxide Resin specially formulated to take the place of ERL 4206 which is no longer available.




Ethyl Alcohol, Anhydrous

Prod #DescriptionUnitPriceOrder / Quote
19206
Ethyl Alcohol, Anhydrous, 1 Liter, A.C.S., glass distilled
each
$102.55
Qty:
F, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
C2H5OH; [64-17-5]; FW 46.07

Prod #DescriptionUnitPriceOrder / Quote
19207
Ethyl Alcohol, Anhydrous, 200ml, A.C.S., glass distilled
each
$19.95
Qty:
F, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees





Formaldehyde, 16%

Prod #DescriptionUnitPriceOrder / Quote
18505
Formaldehyde, 16%, 10 x 10ml
each
$26.40
Qty:
Cg, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
PELCO® Technical Notes, Formaldehyde, Formalin (pdf)
HCHO; [50-00-0]; FW 30.03
EM grade. Paraformaldehyde solution (methanol free). Pre-scored amber ampules sealed under inert gas.

Prod #DescriptionUnitPriceOrder / Quote
18505-100
Formaldehyde, 16%, 100ml
each
$24.60
Qty:
Cg, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
PELCO® Technical Notes, Formaldehyde, Formalin (pdf)
HCHO; [50-00-0]; FW 30.03
EM grade. Paraformaldehyde solution (methanol free). 100ml.

Formaldehyde, 37%

Prod #DescriptionUnitPriceOrder / Quote
18508
Formaldehyde, 37%, microfiltered (Formalin), 10 x 10ml
each
$32.90
Qty:
Cg, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
PELCO® Technical Notes, Formaldehyde, Formalin (pdf)
Histological Fixative
(35 - 38% Formaldehyde, 10 - 15% Methanol)




Formalin, 10% Neutral Buffered in Pre-Fill Containers

Formalin 10% Neutral Buffered in Pre-Fill Containers This traditional fixative is buffered to a neutral pH. It is used in histological applications for fixing and storing tissue specimens. This line of all plastic containers is designed specifically for the collection and transportation of various sizes of tissue samples. Each container is half filled with 10% neutral buffered formalin. The sizes offered are 20ml and 120ml. They are packaged in the proprietary Sure-Lok system. These containers are 95 kPa compliant.

Prod #DescriptionUnitPriceOrder / Quote
18511
NB Pre-Fill Formalin 20ml Container
case/128
$50.75
Qty:
18512
NB Pre-Fill Formalin 20ml Container
case/780
277.60
Qty:
18513
NB Pre-Fill Formalin 40ml Container
case/50
21.15
Qty:
18514
NB Pre-Fill Formalin 60ml Container
case/50
21.75
Qty:
18515
NB Pre-Fill Formalin 90ml Container
case/50
28.10
Qty:
Cg, T (highly) - MSDS
PELCO® Technical Notes, Formaldehyde, Formalin (pdf)

Histological Fixative. Phosphate Buffer. pH 6.9 - 7.1 at 25°C.

Note: ACS Reagent Grade Formalin (35 - 38% Formaldehyde, 10 - 15% Methanol) larger quantities available on request.





Formalin, 10% Neutral Buffered

Prod #DescriptionUnitPriceOrder / Quote
18510
Formalin, 10% Neutral Buffered, 450ml
each
$15.98
Qty:
Cg, T (highly) - MSDS
PELCO® Technical Notes, Formaldehyde, Formalin (pdf)

Histological Fixative. Phosphate Buffer. pH 6.9 - 7.1 at 25°C.

Note: ACS Reagent Grade Formalin (35 - 38% Formaldehyde, 10 - 15% Methanol) larger quantities available on request.




Formvar®

see
Vinylec® (Formvar® substitute)

Gelatin, Purified

Prod #DescriptionUnitPriceOrder / Quote
19225
Gelatin, Purified, 100g
each
$64.00
Qty:

U - MSDS
[9000-70-8]
Coating glass slides - adhesive. Cryosectioning16.
16) Hartmann R: A new embedding medium for cryosectioning eggs of high yolk and lipid content. Eur J Cell Biol, 34, 206, 1984




Glutaraldehyde

glutaraldehyde EM grade purified glass distilled Glutaraldehyde. Available in convenient ampoules of 2 and 10ml and economy bottles of 100ml. Ampoules are sealed with inert gas.     open glutaraldehyde with ampoule breakers Click here to see
Ampoule Breakers

Prod #DescriptionUnitPriceOrder / Quote
18420
Glutaraldehyde, 8% EM grade, 10 x 2ml
each
$20.90
Qty:
C, T, L - MSDS
18421
Glutaraldehyde, 8% EM grade, 10 x 10ml
each
27.40
Qty:
C, T, L - MSDS
18422
Glutaraldehyde, 8% EM grade, 100ml
each
21.40
Qty:
C, T, L - MSDS
18426
Glutaraldehyde, 25% EM grade, 10 x 10ml
each
31.40
Qty:
C, T, L - MSDS
18427
Glutaraldehyde, 25% EM grade, 100ml
each
26.70
Qty:
C, T, L - MSDS
18431
Glutaraldehyde, 50% EM grade, 10 x 10ml
each
49.65
Qty:
C, T, L - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
18432
Glutaraldehyde, 50% EM grade, 100ml
each
49.20
Qty:
C, T, L - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
18436
Glutaraldehyde, 70% EM grade, 10 x 2ml
each
38.80
Qty:
C, T, L - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees






GridStick™ Adhesive

Prod #DescriptionUnitPriceOrder / Quote
155-9
GridStick™ Adhesive, 5ml
each
$12.50
Qty:
F, I, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
Used to adhere SynapTek™ grids to the GridStick™.




Hexamethyldisilazane (HMDS)

Prod #DescriptionUnitPriceOrder / Quote
18605
Hexamethyldisilazane (HMDS), 250ml
each
$78.00
Qty:
C, F, I, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
(CH3)3SiNHSi(CH3)3; [999-97-3]; FW 161.40
Drying tissues for SEM. Stain Tech, 58(6), 347, 1983. Possible substitute for Peldri ll®





Hematoxylin, Certified

Prod #DescriptionUnitPriceOrder / Quote
18721
Hematoxylin, Certified, C.I. #75290, 10g
each
$31.50
Qty:
I - MSDS
C16H14O6; [517-28-2]; FW 302.29
(Natural Black l, Hydroxybrasilin) Solubility: 10.0% Water; 10.0% Alcohol; 9.5% Cellosolve; 10% Glycol; 0.0% Xylene.
A certified biological stain used in histology and cytology and plastic embedded tissues. Chen, Chang, S., J Arch. of Path., 93, 334 (1972).





Immersion Oil

immersion oil

Prod #DescriptionUnitPriceOrder / Quote
19552
Cargille Immersion Oil, Type A, low visc, 120ml (4 oz.)
each
$12.90
Qty:
U MSDS Low viscosity (150cs), low fluorescence, PCB-free.
19556
Cargille Immersion Oil, Type B, high visc, 120ml (4 oz.)
each
14.65
Qty:
U MSDS High viscosity (1250cs), low fluorescence, PCB-free.
19562
Cargille Immersion Oil, Type NVH, very high viscosity, 120ml (4 oz.)
each
16.95
Qty:
U MSDS Very high viscosity (21000cs), low fluorescence, PCB-free.
19564
Cargille Immersion Oil, Type LDF, 30ml (1 oz.)
each
12.50
Qty:
U MSDS Very high viscosity (21000cs), low fluorescence, PCB-free.

immersion oil 5 pack

Prod #DescriptionUnitPriceOrder / Quote
19568
Cargille Immersion Oil, 5-Pack, 5 x 7.4cc (1/4 oz.), 1 ea. Type 300, 1 ea. Type NVH, 2 ea. Type A, 1 ea. Type B
each
$13.90
Qty:

U
Keeps all types handy (A, B, NVH and Type 300).

More about Cargille Microscope Immersion Oils




JB-4® Embedding Kits

Prod #DescriptionUnitPriceOrder / Quote
18020
JB-4® Embedding Kit
each
$399.50
Qty:
F, I, T - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees

Prod #DescriptionUnitPriceOrder / Quote
18040
JB-4® Plus Embedding Kit
each
$342.20
Qty:
F, I, T - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees





LR Gold Resin

Prod #DescriptionUnitPriceOrder / Quote
18183
LR Gold Resin, 500ml
each
$164.50
Qty:
I - MSDS
Resin (acrylic) only, no catalyst included. Requires Catalyst Prod. No. 18186: UV, benzoin methyl ether MSDS (~365nm) (0.5% w/v); "blue light", benzyl MSDS (~460nm) (0.1% w/v); benzoyl peroxide MSDS(1% w/v), cold-cure. Accurate measurements of catalyst are very important. LR Gold can be used at low temperatures (-25°C) and has proven very useful for immunogold procedures. Very low viscosity: 8cP. Applied to both LM (enzyme histochemistry) and EM (immunochemistry) techniques.
Technical Notes (PDF 1,816KB)




Benzoin Methyl Ether

Prod #DescriptionUnitPriceOrder / Quote
18186
Benzoin Methyl Ether, 10g
each
$50.75
Qty:
T, I - MSDS
[3524-62-7]; MW 226.3
UV Catalyst for Prod. No. 18183 LR Gold; use at 0.5% w/v, ~365nm.




LR White Resin

lr white

Prod #DescriptionUnitPriceOrder / Quote
18181
LR White Resin, Medium, 500ml
each
$143.44
Qty:
I - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
Resin with initiator (BPO) packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR™ as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds).

Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions could be seen on the complimentary surface. Epon sections show relatively smooth surfaces.18 Micrographs appear similar to freeze-etched images but scaled to nm instead of µm. Viscosity ~= 8cP

18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470

Landmark Paper, Freeze-Fracture
Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957

Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987.
Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, V. 2, (Hayat MA, ed)

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod #DescriptionUnitPriceOrder / Quote
18182
LR White Resin, Hard, 500ml
each
$131.35
Qty:
I - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
Resin with catalyst packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly close BEEM® Capsules and PTFE Molds with ACLAR® as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results.

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod #DescriptionUnitPriceOrder / Quote
18185
Accelerator for LR white, 10ml
each
$13.50
Qty:
T - MSDS





Lead Citrate, Trihydrate

Prod #DescriptionUnitPriceOrder / Quote
19312
Lead Citrate, Trihydrate, 10g
each
$19.50
Qty:
I, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
Pb3(C6H5O7); 3H2O; [512-26-5]; FW 1053.82

Prod #DescriptionUnitPriceOrder / Quote
19314
Lead Citrate, Trihydrate, 50g
each
$58.35
Qty:
 I, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees




Lowicryl HM-20 MonoStep (non polar)

Prod #DescriptionUnitPriceOrder / Quote
18174
Lowicryl HM-20 MonoStep (non polar), 225g Pre-mixed, ready to use embedding media
each
$292.25
Qty:
I, O, T - MSDS




Methylamine Tungstate

Prod #DescriptionUnitPriceOrder / Quote
18353
Methylamine Tungstate, 1g
each
$83.90
Qty:
U - MSDS
An excellent negative stain. Unlike phosphotungstic Acid, it does not damage virus particles and it is consequently valuable for staining delicate viruses.

Faberge AC, and Oliver RM, 1974. Methylamine Tungstate, a New Negative Stain. J. Microscopy 20, pp. 241-246.




NMA

Prod #DescriptionUnitPriceOrder / Quote
18032
NMA, 450g
each
$21.90
Qty:
C, I - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
C10H10O3; [25134-21-8]; FW 178.19
Methyl-5-norbornene-2,3-dicarboxylic anhydride. Moisture sensitive. Viscosity 225-275cp, density 1.24g/ml. Hardener for epoxy resins.




Nonenyl Succinic Anhydride, (NSA)

Prod #DescriptionUnitPriceOrder / Quote
18301
Nonenyl Succinic Anhydride, (NSA), 450g
each
$33.35
Qty:
I - MSDS
[28928-97-4]
Double-distilled. Epoxy resin hardener.




Osmium Tetroxide

osmum tetroxide     open osmium tetroxide with ampoule breakers
Click here to see
Ampoule Breakers
O, T (highly) - MSDS (4% aqueous)
O, T (highly) - MSDS (crystal)
OsO4; [20816-12-0]; FW 254.20
Solid vaporizes by sublimation. Should be used only under the hood. Affects eyes. Use eye protection.
EM Grade, glass ampoules in black paper. No sticky labels. Osmium purity >99.95%. Light sensitive.

Special note on handling Osmium Tetroxide (reference 5, appendices A:2.5, "Books" , Procedures in Electron Microscopy)

"Great care must be taken with osmium tetroxide. The crystals melt at 40°C and are extremely slow to dissolve in water. Both the crystals and diluted aqueous solutions give off fumes of osmium tetroxide, even at 0°C. It has an extremely low TLV (0.0002ppm). Osmium fixatives must therefore always be handled in a fume hood (cupboard), and because they act so rapidly, skin contact must be avoided at all times."

TLV= Eight hour average Threshold Limit Value measured in milligrams per cubic meter of air.

Prod #DescriptionUnitPriceOrder / Quote
18459
Osmium Tetroxide, 4% aqueous, 10 x 2ml
each
$63.60
Qty:
O, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
18466
Osmium Tetroxide, 4% aqueous, 10 x 5ml
each
118.00
Qty:
O, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product.Hazard Shipping Fees
18463
Osmium Tetroxide, 4% aqueous, 10 x 5ml
each
100.00
Qty:
O, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product.Hazard Shipping Fees
18465
Osmium Tetroxide, 4% aqueous, 10 x 10ml
each
190.00
Qty:
O, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product.Hazard Shipping Fees
18450
Osmium Tetroxide, 1/4 gram crystal, pkg/10
each
166.00
Qty:
O, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product.Hazard Shipping Fees
18451
Osmium Tetroxide, 1/2 gram crystal
each
21.00
Qty:
O, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product.Hazard Shipping Fees
18456
Osmium Tetroxide, 1 gram crystal
each
35.00
Qty:
O, T (highly) - MSDS - There are Hazard Shipping Fees associated with this product.Hazard Shipping Fees





Paraformaldehyde, EM Grade

Prod #DescriptionUnitPriceOrder / Quote
18501
Paraformaldehyde, EM Grade, Prill Purified, 1kg
each
$23.95
Qty:
T, F, I, Cg - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees

(HO(CH2O)nH; [30525-89-4]; FW~600
Fixative. Polyoxymethylene. White free-flowing solid prill with strong, pungent formaldehyde odor. Avoid strong oxidizing agents, temperatures above 38°C.

Karnovsky MJ: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol, 27, 137A, 1965

Note: Karnovsky's 1965 fixative contains 5% glutaraldehyde and 4% formaldehyde in a sodium cacodylate buffer and is very hypotonic (p. 5:1.17, alternative solution strength and formulation suggested)5. (Reference 5, p. 64)

Robertson JD: The occurrence of a subunit pattern in the unit membranes of club endings in Maunther cell synapses in gold fish brain. J Cell Bio, 19, 201, 1963




PELCO Pro CA44 Tissue Adhesive

PELCO® Pro CA44 Tissue Adhesive

PELCO® Pro CA44 is an instant tissue adhesive based on cyanoacrylate. Developed to glue tissue on specimen holders used in vibrating microtomes, such Microslicer and Vibratome. Although the tissue adhesive is instable in boiling water, it is recommended to use #19920 PELCO® Pro Cyanoacrylate Debonder (below) to remove or clean cured cyanoacrylate. Supplied in 20ml bottle with dispenser tip, closed with ptfe coated steel pin for optimum seal.
Compatible to Loctite 404 tissue adhesive.


Prod #DescriptionUnitPriceOrder / Quote
10033
PELCO® Pro CA44 Tissue Adhesive, 20ml
each
$9.60
Qty:
I - MSDS




PELCO Cyanoacrylate Debonder

PELCO® Pro Cyanoacrylate Debonder

The PELCO® Pro Cyanoacrylate Debonder destabilizes and debonds cured cyanoacrylates. Useful for releasing specimens from holders, cleaning cyanoacrylates from surfaces and removing cured cyanoacylates from fingers. Works on all brands of cyanoacrylate glue (Loctite, Henkel, Permabond, 3M™, PELCO®, Aron Alpha, etc.). The debonder is also a solvent, please check before using on plastic surfaces / samples and avoid repeated skin contact. Supplied in 20ml bottle with dispenser tip.


Prod #DescriptionUnitPriceOrder / Quote
19920
PELCO® Pro Cyanoacrylate Debonder, 20ml
each
$5.69
Qty:
F, Cg, I, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees




Phosphotungstic Acid, Reagent (PTA)

Prod #DescriptionUnitPriceOrder / Quote
19402
Phosphotungstic Acid, Reagent (PTA), 25g
each
$40.85
Qty:
T, C - MSDS
12WO3H3PO4; xH2O; [12067-99-1]; FW 2880




Pioloform Powder

Prod #DescriptionUnitPriceOrder / Quote
19244
Pioloform Powder, 10g
each
$6.90
Qty:
I - MSDS
Substrate component. Water-insoluble. Good thermal and mechanical stability. Because of small mass thickness, electron scattering within the film is low. 0.3-1.5% solution in chloroform, filtered through filter paper.





Poly-L-Lysine

poly-l-lysine

Prod #DescriptionUnitPriceOrder / Quote
18021
Poly-L-Lysine solution, 500µl
each
$2.99
Qty:
18026
Poly-L-Lysine solution, 10ml
each
12.95
Qty:
U MSDS
0.1% w/v aqueous solution; preservative. Adhesive for tissue sections on glass slides. Immunohistochemical techniques, gold labeling, microwave procedures.





Propylene Oxide, EM Grade

Prod #DescriptionUnitPriceOrder / Quote
18601
Propylene Oxide, EM Grade, 450ml
each
$25.90
Qty:
Cg, F, I Special Caution - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees

CH3CHCH2O [75-56-9] FW 58.08
1,2-epoxypropane. Must use under hood, and with all caution. Extremely low vapor pressure. "Since epoxy resins are soluble in ethanol and acetone, dehydration is commonly carried out with one of these solvents. Because epoxy resins are more readily soluble in propylene oxide. This solvent is frequently used immediately prior to infiltration with the resin. Most resins are not readily miscible with ethanol". Propylene Oxide does not separate from the epoxy resin during curing, thus small amounts left in the resin do no harm.

Luft introduced Propylene Oxide into epoxy mixtures with his landmark paper and helped bring about improved embedding cures, better ultrastructural research results with blocks that would more reliably section and give better performance under the EM beam by reducing viscosity and increasing penetration.




Ruthenium Red

Prod #DescriptionUnitPriceOrder / Quote
19421
Ruthenium Red, 1g
each
$67.45
Qty:
I - MSDS
[(NH3)5 RuoRu(NH3) 4 ORu(NH3)5]Cl6 · 4H2O
[11103-72-3]; FW 786.36




Sodium Hydroxide, Pellets, Reagent, ACS

Prod #DescriptionUnitPriceOrder / Quote
19539
Sodium Hydroxide, Pellets, Reagent, ACS, 500g
each
$25.50
Qty:
C, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
NaOH, [1310-73-2]; FW 40




Sodium Hydroxide, 2N Solution, carbonate-free

Prod #DescriptionUnitPriceOrder / Quote
19540
Sodium Hydroxide, 2N Solution, carbonate-free, 225ml
each
$15.60
Qty:
C, T - MSDS - There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees
NaOH; [1310-73-2]; FW 40
Normality 1.98­2.02, pH 8.6. Recommended: Bils RF: Electron Microscopy Laboratory Manual and Handbook, 2nd ed., 1992, p 154.




Sodium Phosphate, monobasic, anhydrous, Reagent, ACS

Prod #DescriptionUnitPriceOrder / Quote
19542
Sodium Phosphate, monobasic, anhydrous, Reagent, ACS, 1000g
each
$38.80
Qty:
I - MSDS
NaH2PO4 ; [7558-80-7]; FW 119.98
Hygroscopic.




Sodium Phosphate, dibasic, heptahydrate, Reagent, ACS

Prod #DescriptionUnitPriceOrder / Quote
19545
Sodium Phosphate, dibasic, heptahydrate, Reagent, ACS, 450g
each
$32.50
Qty:
I - MSDS
Na2HPO4 · 7H2O; [7782-85-6]; FW 268.07




Tissue-Tack Adhesive

Prod #DescriptionUnitPriceOrder / Quote
19250
Tissue-Tack Adhesive, 100ml
each
$85.50
Qty:
I - MSDS
Permanent attachment of sections to glass slides during caustic or prolonged washes. For routine, immunofluorescent, immunoperoxidase staining.




Toluidine Blue O

Prod #DescriptionUnitPriceOrder / Quote
19451
Toluidine Blue O, certified, 25g
each
$29.80
Qty:
I, T - MSDS
C15H16CLN3 S; [92-31-9]
For epoxy sections:

0.5% toluidine blue in 0.1% sodium carbonate solution at pH 11.1

For methacrylate/acrylic sections:
0.05% toluidine blue in benzoate buffer pH 4.4 (benzoic acid 0.25g, sodium benzoate 0.29g, water 200ml). p 5:3.11, "Procedures in Electron Microscopy".




Uranyl Acetate

Prod #DescriptionUnitPriceOrder / Quote
19481
Uranyl Acetate, 25g
each
$154.90
Qty:
T, R (depleted) - MSDS
PELCO® Technical Notes, Uranium Salts for Staining, Uranyl Acetate (pdf)
UO2(CH3COO)2 - 2H2O; [6159-44-0]; FW 424.14
Positive or negative stain, EM. May cause adverse mutagenic or teratogenic effects.




Vinylec®

(Formvar® substitute)

Prod #DescriptionUnitPriceOrder / Quote
19223
Vinylec® - Formvar® substitute, 100g
each
$12.00
Qty:
I - MSDS soon to come
Vinylec® is a drop in substitute for Formvar® (polyvinyl formal). The original manufacturer (Monsanto) has sold parts of the business and the same product is now manufactured under the name Vinylec®.
Vinylec® (Formvar® substitute) is an excellent and widely used material to make support films on TEM grids.