in 



Chemicals Index

Chemicals for Electron and Light Microscopy

in alphabetical order


Hazard Warning Guides Used in the Above Listings

Cg = Suspected Carcinogen
C = Corrosive
F = Flammable
I = Irritant
L = Lachrymator
O = Oxidizing
R = Radioactive
S = Sensitizer
T = Toxic
U = Unlisted as hazard; unknown hazard if any

An extra shipping charge is required by the Carriers when hazardous goods are transported.




Acetonitrile

Prod # Description Unit Price Order / Quote
18612 Acetonitrile, 450ml each $56.00
Qty:
F, T - MSDS (106KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
CH3CN; [75-05-08]; FW 41.05

Suggested substitute for Propylene Oxide. It is reported as noncarcinogenic, less toxic and not as flammable. High purity; miscible with water, alcohols, acetone and epoxy resins15.

15) Tarnowski BI, Schonbaum GR: Acetonitrile: substitute for propylene oxide in tissue processing for transmission electron microscopy. Proc 42nd Ann Meeting Elec Mic Soc Amer, p 38, 1984

Edwards HH, Yeh YY, Tarnowski BI, Schonbaum GR: Acetonitrile as a substitute for ethanol/propylene oxide in tissue processing for transmission electron microscopy: Comparison of fine structure and lipid solubility in mouse liver, kidney, and intestine. Micro Res & Tech, 21, pp 39­50, 1992

Also used as a dehydrating agent for cells prepared for SEM: Holshek JG, Akins RE: Acetonitrile is better than ethanol as a dehydrating agent for cells prepared for SEM. Proc Mic Soc of Amer, 52nd Annual Mtg 1994, San Francisco Press, ed GW Bailey and AJ Garrett-Reed, pp 324-325, 1994






Araldite 502

Prod # Description Unit Price Order / Quote
18060 Araldite 502, 450g each $20.35
Qty:
I, T - MSDS (91KB PDF)
Detailed description under Araldite 502 Kit.





Background Buster

Prod # Description Unit Price Order / Quote
18722-50 Background Buster, 50ml each $327.85
Qty:
Detailed description under Background Buster.




Benzoin Methyl Ether

Prod # Description Unit Price Order / Quote
18186 Benzoin Methyl Ether, 10g each $62.50
Qty:
T, I - MSDS (106KB PDF)
[3524-62-7]; MW 226.3
UV Catalyst for Prod. No. 18183 LR Gold; use at 0.5% w/v, ~365nm.





Benzyldimethylamine

Prod # Description Unit Price Order / Quote
18241 Benzyldimethylamine, 25g each $9.35
Qty:
C, T, I, F - MSDS (113KB PDF)
C6H5CH2N(CH3)2; [103-83-3]; FW 135.21
(BDMA) N,N-Dimethylbenzylamine Catalyst for epoxy resins. Has lower viscosity compared to DMP-30. (Also stores longer.)




Cacodylic Acid, Sodium Salt, trihydrate

Prod # Description Unit Price Order / Quote
18851 Cacodylic Acid, Sodium Salt, trihydrate, 100g each $126.70
Qty:
T (highly) - MSDS (106KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
(CH3)2As(O)ONa-3H2O; [124-65-2]; FW 214.02;UN1688
Arsenic compound. Highly toxic. Hygroscopic.
Sabatini DD, Miller F, Barrnett RJ: Cytochemistry and electron microscopy. The preservation of cellular structure and enzymatic activity by aldehyde fixation. J Cell Biol, 17, 19­58, 1963




Citifluor Mountant Media

Complete description and micrograph examples

Prod # Description Unit Price Order / Quote
Citifluor Antifadent (AF) Solutions
19470 AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 25ml each $76.50
Qty:
19470-1 AF-1, Glycerol-phosphate buffered solution, containing antifadent for use with labelled tissue sections and dead cells, 100ml each 216.75
Qty:
I - MSDS (117KB PDF)
19472 AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 25ml each 76.50
Qty:
19472-1 AF-2, Glycerol solution containing antifadent for use with labelled tissue sections and dead cells, 100ml each 216.75
Qty:
I - MSDS (112KB PDF)
Glycerol solution, additives.
19474 AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 25ml each 76.50
Qty:
19474-1 AF-3, Phosphate-buffered saline solution containing antifadent for examination of whole live cells, 100ml each 216.75
Qty:
I - MSDS (112KB PDF)
19476-A Citifluor non-fluorescent Immersion Oil, AFB7, 10ml each 69.50
Qty:
I - MSDS (110KB PDF)
19476 AF-4, Glycerol solution containing n-propyl gallate for use with labelled tissue sections and dead cells, 10ml each 76.50
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19490 AF-100, Phosphate-buffered saline solution containing antifadent specially prepared for use with UVM23 solution where fluorochrome fading is a problem, 5ml each 14.20
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I - MSDS (111KB PDF)
19491 AF-200, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used with other fluorochromes such as fluorescein, Alexa dyes and Hoescht dye, 10ml each 74.50
Qty:
I - MSDS (111KB PDF)
19492 AF-300, Glycerol solution containing antifadent, specially prepared for use with UVM2 solution where DAPI is the staining material. The solutions can also be used where fluorescein is the staining material. The solution can also be used with other fluorochromes such as rhodamines, etc., 10ml each 74.50
Qty:
I - MSDS (111KB PDF)
19493 CFM1, Glycerol-PBS ref. index similar to glass, 10ml each 51.75
Qty:
I - MSDS (112KB PDF)
19494 CFM1, Glycerol-PBS w/AF, ref. index similar to glass, 10ml each 61.20
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I - MSDS (pdf 135K)
19495 CFM2, Glycerol-TRIS buffer, ref. index similar to glass, 10ml each 51.75
Qty:
I - MSDS (pdf 110K)
19496 CFM3, Glycerol-Phosphate & AF, ref. index similar to glass, 10ml each 59.50
Qty:
I - MSDS (112KB PDF)
UV Mount Media (UVM)
19497 UVM1, Methacrylate based solution, UV curable, gives clear film, 10ml each 51.75
Qty:
I - MSDS (69KB PDF)
19498 UVM2, Methacrylate based solution, contains anti-bleaching agent, 10ml each 51.75
Qty:
I - MSDS 90KB PDF)
19499 UVM3, High refractive index methacrylate-based solution designed to be used with AF-100 where fluorochrome fading is a problem, 10ml each 71.20
Qty:
I - MSDS (68KB PDF)
Solid Mountants
19469 Citifluor Solid Mountant Kit is comprised of PVA solution, 25ml and Citifluor antifadent in buffer, 5ml. The components can be mixed as required, applied to the specimen and allowed to dry to give a clear film, 30ml each 95.00
Qty:
C - MSDS for Citifluor AF100 Antifadent Solution, 25ml (116KB PDF)
U - MSDS for Citifluor CFPVOH Solution, 5ml (113KB PDF)
Contains anti-fading solution. Suitable for preventing fading of dyes such as DAPI which have the tendency to disperse in glycerol based mounting solutions.





Cresyl (Fast) Violet, Certified

Prod # Description Unit Price Order / Quote
18709 Cresyl (Fast) Violet, Certified, C.I. #51180, 5g each $93.55
Qty:
U - MSDS (98KB PDF)
C18H15N3O3 [10510-54-0] FW 312.34
(Cresyl Violet Acetate) Solubility: 0.13% water
Nguyen KB and Pender MP, 1989. Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining. Stain Technology 64:4, pp. 163-167.





DAPI, fluorescent Dye

Prod # Description Unit Price Order / Quote
18706 DAPI, fluorescent Dye (powder), 10mg each $202.50
Qty:
I - MSDS (100KB PDF)
C16H15N5•2HCl; [28718-90-3]; FW 350.3
Cell permeable fluorescent minor grove-binding probe for DNA (powder).
4',6-Diamidino-2-phenylindole Dihydrochloride:Hydrate





Deionized Water

Prod # Description Unit Price Order / Quote
18708 Deionized Water, 1 gallon each $10.70
Qty:
For general laboratory use





Diglycidyl Ether of Poly (propylene glycol) (DER 736)

Prod # Description Unit Price Order / Quote
18310 Diglycidyl Ether of Poly (propylene glycol) (DER 736), 250g each $20.75
Qty:
I - MSDS (99KB PDF)
[41638-13-5]; Specific gravity 1.14.; Epoxy flexibilizer; Viscosity 33.6; Cp @ 25°C.





2-Dimethylaminoethanol (DMAE)

Prod # Description Unit Price Order / Quote
18315 2-Dimethylaminoethanol (DMAE), 25g each $8.20
Qty:
C, L - MSDS (93KB PDF)
C4H11NO; [108-01-0]; FW 89.14





2,4,6-Tri[Dimethylaminomethyl]phenol) (DMP-30)

Prod # Description Unit Price Order / Quote
18042 (2,4,6-Tri[Dimethylaminomethyl]phenol) (DMP-30), 25g each $5.90
Qty:
I - MSDS (100KB PDF)
[90-70-2]; FW 265.0
Tertiary amine accelerator.
Inactivated by water. Store in dark in vacuum desiccator.
DMP dehydration for EM:
Rapid Chemical Dehydration of Samples for Electron Microscopic Examinations. L.L. Muller and T.J. Jacks. The Journal of Histochemistry and Cytochemistry. Vol. 23, No. 2, pp.107-110, 1975.
2,2-Dimethoxypropane, a rapid dehydrating agent for scanning electron microscopy. W.S.Johnson, G.R. Hooper, B.F. Holdaway, and H.P. Rasumssen.
Micron, 1976, Vol. 7:305-306.
Rapid Chemical Dehydration of Biologic Samples for Scanning Electron Microscopy using 2,2-Dimethoxypropane. Morton D. Maser and John J. Trimble, III. The Journal of Histochemistry and Cytochemistry. Vol. 25, No. 4, pp. 247-251.
1977.
Optimization and Investigation of the Use of 2,2-Dimethoxypropane as a Dehydration Agent for Plant Tissues in Transmission Electron Microscopy. J.R. Thorpe and Diana M.R. Harvey.
Journal of Ultrastruture Research, 68, 186-194 (1979).





Dodecenyl Succinic Anhydride (DDSA)

Prod # Description Unit Price Order / Quote
18022 Dodecenyl Succinic Anhydride (DDSA), 450g each $32.20
Qty:
I - MSDS (98KB PDF)
[26544-38-7]; FW 266.38
Double-distilled. Moisture sensitive. Hardener for epoxy resins. Viscosity 290-295cP, density 1.005g/ml.




Eponate 12™ Resin

Prod # Description Unit Price Order / Quote
18005 Eponate 12™ Resin, 450g each $68.20
Qty:
I, Cg - MSDS (88KB PDF)
[25038-04-4]
Substitute for "Epon®" recipes and our Medcast. Excellent results with traditional EM specimen block preparations. WPE 140-150. Viscosity @ 25°'C 130-170cP. Specific Gravity 1.24. Moisture sensitive.

1,2-epoxypropane (see Propylene Oxide) MSDS (116KB PDF)




ERL 4221 Epoxide Resin

Prod # Description Unit Price Order / Quote
18306-4221 ERL 4221 Epoxide Resin, 225ml each $28.00
Qty:
Cg - MSDS (118KB PDF)
C14H20O4; [2386-87-0; 2611-00-9]; FW 1.173
A Cycloaliphatic Epoxide Resin specially formulated to take the place of ERL 4206 which is no longer available.




Ethyl Alcohol, Anhydrous

Prod # Description Unit Price Order / Quote
19206 Ethyl Alcohol, Anhydrous, 1 Liter, A.C.S., glass distilled each $108.75
Qty:
F, T - MSDS (48KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
C2H5OH; [64-17-5]; FW 46.07

Prod # Description Unit Price Order / Quote
19207 Ethyl Alcohol, Anhydrous, 200ml, A.C.S., glass distilled each $19.95
Qty:
F, T - MSDS (48KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees





Formaldehyde, 16%

Prod # Description Unit Price Order / Quote
18505 Formaldehyde, 16%, 10 x 10ml each $28.00
Qty:
Cg, T (highly) - MSDS (94KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
PELCO® Technical Notes, Formaldehyde, Formalin (PDF)
HCHO; [50-00-0]; FW 30.03
EM grade. Paraformaldehyde solution (methanol free). Pre-scored amber ampules sealed under inert gas.

Prod # Description Unit Price Order / Quote
18505-100 Formaldehyde, 16%, 100ml each $32.00
Qty:
Cg, T (highly) - MSDS (94KB PDF)- There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
PELCO® Technical Notes, Formaldehyde, Formalin (PDF)
HCHO; [50-00-0]; FW 30.03
EM grade. Paraformaldehyde solution (methanol free). 100ml.

Formaldehyde, 37%

Prod # Description Unit Price Order / Quote
18508 Formaldehyde, 37%, microfiltered (Formalin), 10 x 10ml each $42.00
Qty:
Cg, T (highly) - MSDS (101KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
PELCO® Technical Notes, Formaldehyde, Formalin (PDF)
Histological Fixative
(35 - 38% Formaldehyde, 10 - 15% Methanol)





Formalin, 10% Neutral Buffered

Prod # Description Unit Price Order / Quote
18510 Formalin, 10% Neutral Buffered, 450ml each $15.98
Qty:
Cg, T (highly) - MSDS (120KB PDF)
PELCO® Technical Notes, Formaldehyde, Formalin (PDF)

Histological Fixative. Phosphate Buffer. pH 6.9 - 7.1 at 25°C.

Note: ACS Reagent Grade Formalin (35 - 38% Formaldehyde, 10 - 15% Methanol) larger quantities available on request.




Formvar®

see
Vinylec® (Formvar® substitute)

Gelatin, Purified

Prod # Description Unit Price Order / Quote
19225 Gelatin, Purified, 100g each $69.35
Qty:

U - MSDS (94KB PDF)
[9000-70-8]
Coating glass slides - adhesive. Cryosectioning16.
16) Hartmann R: A new embedding medium for cryosectioning eggs of high yolk and lipid content. Eur J Cell Biol, 34, 206, 1984




Glutaraldehyde

glutaraldehyde EM grade purified glass distilled Glutaraldehyde. Available in convenient ampoules of 2 and 10ml and economy bottles of 100ml. Ampoules are sealed with inert gas.     open glutaraldehyde with ampoule breakers Click here to see
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Prod # Description Unit Price Order / Quote
18420 Glutaraldehyde, 8% EM grade, 10 x 2ml each $25.35
Qty:
There are Hazard Shipping Fees associated with this product. -
C, T, L - MSDS (96KB PDF)
18421 Glutaraldehyde, 8% EM grade, 10 x 10ml each 32.90
Qty:
There are Hazard Shipping Fees associated with this product. -
C, T, L - MSDS (96KB PDF)
18422 Glutaraldehyde, 8% EM grade, 100ml each 26.70
Qty:
There are Hazard Shipping Fees associated with this product. -
C, T, L - MSDS (96KB PDF)
18426 Glutaraldehyde, 25% EM grade, 10 x 10ml each 32.65
Qty:
There are Hazard Shipping Fees associated with this product. -
C, T, L - MSDS (116KB PDF)
18427 Glutaraldehyde, 25% EM grade, 100ml each 28.00
Qty:
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C, T, L - MSDS (116KB PDF)
18431 Glutaraldehyde, 50% EM grade, 10 x 10ml each 53.35
Qty:
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C, T, L - MSDS (132KB PDF)
18432 Glutaraldehyde, 50% EM grade, 100ml each 52.00
Qty:
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C, T, L - MSDS (132KB PDF)
18436 Glutaraldehyde, 70% EM grade, 10 x 2ml each 41.50
Qty:
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C, T, L - MSDS (120KB PDF)





GridStick™ Adhesive

Prod # Description Unit Price Order / Quote
155-9 GridStick™ Adhesive, 5ml each $12.50
Qty:
F, I, T - MSDS (120KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
Used to adhere SynapTek™ grids to the GridStick™.




Hexamethyldisilazane (HMDS)

Prod # Description Unit Price Order / Quote
18605 Hexamethyldisilazane (HMDS), 250ml each $78.00
Qty:
C, F, I, T - MSDS (94KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
(CH3)3SiNHSi(CH3)3; [999-97-3]; FW 161.40
Drying tissues for SEM. Stain Tech, 58(6), 347, 1983. Possible substitute for Peldri II®




Immersion Oil

immersion oil

Prod # Description Unit Price Order / Quote
19552 Cargille Immersion Oil, Type A, low visc, 120ml (4 oz.) each $15.75
Qty:
U MSDS (57KB PDF) Low viscosity (150cs), low fluorescence, PCB-free.
19556 Cargille Immersion Oil, Type B, high visc, 120ml (4 oz.) each 15.75
Qty:
U MSDS (56KB PDF) High viscosity (1250cs), low fluorescence, PCB-free.
19562 Cargille Immersion Oil, Type NVH, very high viscosity, 120ml (4 oz.) each 20.95
Qty:
U MSDS (57KB PDF) Very high viscosity (21000cs), low fluorescence, PCB-free.
19563 Cargille Immersion Oil, Type 300, medium viscosity, 120ml (4 oz.) each 19.25
Qty:
U MSDS (57KB PDF) medium viscosity (300cs), low fluorescence, PCB-free.
19564-L Cargille Immersion Oil, Type LDF, 30ml (1 oz.) each 12.50
Qty:
U MSDS (57KB PDF) Very high viscosity (21000cs), low fluorescence, PCB-free.

immersion oil 5 pack

Prod # Description Unit Price Order / Quote
19568 Cargille Immersion Oil, 5-Pack, 5 x 7.4cc (1/4 oz.), 1 ea. Type 300, 1 ea. Type NVH, 2 ea. Type A, 1 ea. Type B each $18.70
Qty:
U
Keeps all types handy (A, B, NVH and Type 300).

More about Cargille Microscope Immersion Oils



JB-4® Embedding Kits

Prod # Description Unit Price Order / Quote
18020 JB-4® Embedding Kit each $436.35
Qty:
F, I, T - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees

Prod # Description Unit Price Order / Quote
18040 JB-4® Plus Embedding Kit each $379.70
Qty:
F, I, T - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees





LR Gold Resin

Prod # Description Unit Price Order / Quote
18183 LR Gold Resin, 500ml each $166.50
Qty:
I - MSDS (108KB PDF)
Resin (acrylic) only, no catalyst included. Requires Catalyst Prod. No. 18186: UV, benzoin methyl ether MSDS (106KB PDF) (~365nm) (0.5% w/v); "blue light", benzyl MSDS (~460nm) (0.1% w/v); benzoyl peroxide MSDS (108KB PDF) (1% w/v), cold-cure. Accurate measurements of catalyst are very important. LR Gold can be used at low temperatures (-25°C) and has proven very useful for immunogold procedures. Very low viscosity: 8cP. Applied to both LM (enzyme histochemistry) and EM (immunochemistry) techniques.
Technical Notes (PDF 1,816KB)




Benzoin Methyl Ether

Prod # Description Unit Price Order / Quote
18186 Benzoin Methyl Ether, 10g each $62.50
Qty:
T, I - MSDS (106KB PDF)
[3524-62-7]; MW 226.3
UV Catalyst for Prod. No. 18183 LR Gold; use at 0.5% w/v, ~365nm.




LR White Resin

lr white

Prod # Description Unit Price Order / Quote
18181 LR White Resin, Medium, 500ml each $154.00
Qty:
I - MSDS (107KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
Resin with initiator (BPO) packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR® as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds).

Sections from LR White appear "bumpy" in contrast to Epon® resin sections. It is possible that Epon® links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions could be seen on the complimentary surface. Epon® sections show relatively smooth surfaces.18 Micrographs appear similar to freeze-etched images but scaled to nm instead of µm. Viscosity ~= 8cP

18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470

Landmark Paper, Freeze-Fracture
Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957

Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987.
Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, V. 2, (Hayat MA, ed)

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod # Description Unit Price Order / Quote
18182 LR White Resin, Hard, 500ml each $154.00
Qty:
I - MSDS (107KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
Resin with catalyst packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly close BEEM® Capsules and PTFE Molds with ACLAR® as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results.

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod # Description Unit Price Order / Quote
18185 Accelerator for LR white, 10ml each $19.50
Qty:
T - MSDS (89KB PDF)




Lead Citrate, Trihydrate

Prod # Description Unit Price Order / Quote
19312 Lead Citrate, Trihydrate, 10g each $19.50
Qty:
I, T - MSDS (124KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
Pb3(C6H5O7); 3H2O; [512-26-5]; FW 1053.82

Prod # Description Unit Price Order / Quote
19314 Lead Citrate, Trihydrate, 50g each $58.35
Qty:
I, T - MSDS (124KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees




Lowicryl® HM-20 MonoStep® (non polar)

Prod # Description Unit Price Order / Quote
18174 Lowicryl® HM-20 MonoStep® (non polar), 225g Pre-mixed, ready to use embedding media each $324.50
Qty:
I, O, T - MSDS (91KB PDF)




Methylamine Tungstate

Prod # Description Unit Price Order / Quote
18353 Methylamine Tungstate, 1g each $92.50
Qty:
U - MSDS (85KB PDF)
An excellent negative stain. Unlike phosphotungstic Acid, it does not damage virus particles and it is consequently valuable for staining delicate viruses.

Faberge AC, and Oliver RM, 1974. Methylamine Tungstate, a New Negative Stain. J. Microscopy 20, pp. 241-246.




NMA

Prod # Description Unit Price Order / Quote
18032 NMA, 450g each $22.90
Qty:
C, I - MSDS (99KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
C10H10O3; [25134-21-8]; FW 178.19
Methyl-5-norbornene-2,3-dicarboxylic anhydride. Moisture sensitive. Viscosity 225-275cp, density 1.24g/ml. Hardener for epoxy resins.




Nonenyl Succinic Anhydride, (NSA)

Prod # Description Unit Price Order / Quote
18301 Nonenyl Succinic Anhydride, (NSA), 450g each $34.65
Qty:
I - MSDS (92KB PDF)
[28928-97-4]
Double-distilled. Epoxy resin hardener.




Osmium Tetroxide

osmum tetroxide     open osmium tetroxide with ampoule breakers
Click here to see
Ampoule Breakers
OsO4; [20816-12-0]; FW 254.20
Solid vaporizes by sublimation. Should be used only under the hood. Affects eyes. Use eye protection.
EM Grade, glass ampoules in black paper. No sticky labels. Osmium purity >99.95%. Light sensitive.

Special note on handling Osmium Tetroxide (reference 5, appendices A:2.5, "Books" , Procedures in Electron Microscopy)

"Great care must be taken with osmium tetroxide. The crystals melt at 40°C and are extremely slow to dissolve in water. Both the crystals and diluted aqueous solutions give off fumes of osmium tetroxide, even at 0°C. It has an extremely low TLV (0.0002ppm). Osmium fixatives must therefore always be handled in a fume hood (cupboard), and because they act so rapidly, skin contact must be avoided at all times."

TLV= Eight hour average Threshold Limit Value measured in milligrams per cubic meter of air.

Prod # Description Unit Price Order / Quote
18459 Osmium Tetroxide, 4% aqueous, 10 x 2ml each $63.60
Qty:
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O, T (highly) - MSDS (121KB PDF) (4% aqueous)
18466 Osmium Tetroxide, 2% aqueous, 10 x 5ml each 118.00
Qty:
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O, T (highly) - MSDS (121KB PDF) (2% aqueous)
18463 Osmium Tetroxide, 4% aqueous, 10 x 5ml each 100.00
Qty:
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O, T (highly) - MSDS (121KB PDF) (4% aqueous)
18465 Osmium Tetroxide, 4% aqueous, 10 x 10ml each 190.00
Qty:
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O, T (highly) - MSDS (121KB PDF) (4% aqueous)
18450 Osmium Tetroxide, 1/4 gram crystal, pkg/10 each 166.00
Qty:
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O, T (highly) - MSDS (293KB PDF) (crystal)
18451 Osmium Tetroxide, 1/2 gram crystal each 21.00
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O, T (highly) - MSDS (293KB PDF) (crystal)
18456 Osmium Tetroxide, 1 gram crystal each 35.00
Qty:
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O, T (highly) - MSDS (293KB PDF) (crystal)




Paraformaldehyde, EM Grade

Prod # Description Unit Price Order / Quote
18501 Paraformaldehyde, EM Grade, Prill Purified, 1kg each $27.95
Qty:
T, F, I, Cg - MSDS (116KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees

(HO(CH2O)nH; [30525-89-4]; FW~600
Fixative. Polyoxymethylene. White free-flowing solid prill with strong, pungent formaldehyde odor. Avoid strong oxidizing agents, temperatures above 38°C.

Karnovsky MJ: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol, 27, 137A, 1965

Note: Karnovsky's 1965 fixative contains 5% glutaraldehyde and 4% formaldehyde in a sodium cacodylate buffer and is very hypotonic (p. 5:1.17, alternative solution strength and formulation suggested)5. (Reference 5, p. 64)

Robertson JD: The occurrence of a subunit pattern in the unit membranes of club endings in Maunther cell synapses in gold fish brain. J Cell Bio, 19, 201, 1963




PELCO Pro CA44 Tissue Adhesive

PELCO® Pro CA44 Tissue Adhesive

PELCO® Pro CA44 is an instant tissue adhesive based on cyanoacrylate. Developed to glue tissue on specimen holders used in vibrating microtomes such as Microslicer™ and Vibratome®. Although the tissue adhesive is unstable in boiling water, it is recommended to use #19920 PELCO® Pro Cyanoacrylate Debonder (below) to remove or clean cured cyanoacrylate. Supplied in 20ml bottle with dispenser tip, closed with ptfe coated steel pin for optimum seal.
Compatible to Loctite 404 tissue adhesive.

Cyanoacrylate Brand Cross Reference and Technical Data
I - 10033 MSDS (88KB PDF)

Prod # Description Unit Price Order / Quote
10033 PELCO® Pro CA44 Instant Tissue Adhesive, 20ml each $9.60
Qty:

The shelf life of cyanoacrylate glues (and epoxies) can be extended by storing them at lower temperatures; -20°C. At lower temperatures, the adhesive becomes very viscous and the speed of chemical reactions is practically reduced to zero. It will keep the adhesive stable for years. Before use, let the adhesive thaw with the container sealed to avoid condensation into the adhesive.





PELCO Cyanoacrylate Debonder

PELCO® Pro Cyanoacrylate Debonder

The PELCO® Pro Cyanoacrylate Debonder destabilizes and debonds cured cyanoacrylates. Useful for releasing specimens from holders, cleaning cyanoacrylates from surfaces and removing cured cyanoacylates from fingers. Works on all brands of cyanoacrylate glue (Loctite®, Henkel, Permabond, 3M™, PELCO®, Aron Alpha, etc.). The debonder is also a solvent, please check before using on plastic surfaces / samples and avoid repeated skin contact. Supplied in 20ml bottle with dispenser tip.


Prod # Description Unit Price Order / Quote
19920 PELCO® Pro Cyanoacrylate Debonder, 20ml each $5.69
Qty:
F, Cg, I, T - MSDS (96KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees




Phosphotungstic Acid, Reagent (PTA)

Prod # Description Unit Price Order / Quote
19402 Phosphotungstic Acid, Reagent (PTA), 25g each $70.00
Qty:
T, C - MSDS (93KB PDF)
12WO3H3PO4; xH2O; [12067-99-1]; FW 2880




Pioloform® Powder

Prod # Description Unit Price Order / Quote
19244 Pioloform® Powder, 10g each $6.90
Qty:
I - MSDS (102KB PDF)
Substrate component. Water-insoluble. Good thermal and mechanical stability. Because of small mass thickness, electron scattering within the film is low. 0.3-1.5% solution in chloroform, filtered through filter paper.





Poly-L-Lysine

poly-l-lysine

Prod # Description Unit Price Order / Quote
18021 Poly-L-Lysine solution, 500µl each $2.99
Qty:
18026 Poly-L-Lysine solution, 10ml each 13.95
Qty:
U MSDS (93KB PDF)
0.1% w/v aqueous solution; preservative. Adhesive for tissue sections on glass slides. Immunohistochemical techniques, gold labeling, microwave procedures.




Propylene Oxide, EM Grade

Prod # Description Unit Price Order / Quote
18601 Propylene Oxide, EM Grade, 450ml each $32.00
Qty:
Cg, F, I Special Caution - MSDS (116KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees

CH3CHCH2O [75-56-9] FW 58.08
1,2-epoxypropane. Must use under hood, and with all caution. Extremely low vapor pressure. "Since epoxy resins are soluble in ethanol and acetone, dehydration is commonly carried out with one of these solvents. Because epoxy resins are more readily soluble in propylene oxide. This solvent is frequently used immediately prior to infiltration with the resin. Most resins are not readily miscible with ethanol". Propylene Oxide does not separate from the epoxy resin during curing, thus small amounts left in the resin do no harm.

Luft introduced Propylene Oxide into epoxy mixtures with his landmark paper and helped bring about improved embedding cures, better ultrastructural research results with blocks that would more reliably section and give better performance under the EM beam by reducing viscosity and increasing penetration.




Ruthenium Red

Prod # Description Unit Price Order / Quote
19421 Ruthenium Red, 1g each $67.45
Qty:
I - MSDS (215KB PDF)
[(NH3)5 RuoRu(NH3) 4 ORu(NH3)5]Cl6 · 4H2O
[11103-72-3]; FW 786.36




Sodium Hydroxide, Pellets, Reagent, ACS

Prod # Description Unit Price Order / Quote
19539 Sodium Hydroxide, Pellets, Reagent, ACS, 500g each $26.80
Qty:
C, T - MSDS (90KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
NaOH, [1310-73-2]; FW 40




Sodium Hydroxide, 2N Solution, carbonate-free

Prod # Description Unit Price Order / Quote
19540 Sodium Hydroxide, 2N Solution, carbonate-free, 225ml each $15.60
Qty:
C, T - MSDS (118KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
NaOH; [1310-73-2]; FW 40
Normality 1.98­2.02, pH 8.6. Recommended: Bils RF: Electron Microscopy Laboratory Manual and Handbook, 2nd ed., 1992, p 154.




Sodium Phosphate, monobasic, anhydrous, Reagent, ACS

Prod # Description Unit Price Order / Quote
19542 Sodium Phosphate, monobasic, anhydrous, Reagent, ACS, 1000g each $48.55
Qty:
I - MSDS (92KB PDF)
NaH2PO4 ; [7558-80-7]; FW 119.98
Hygroscopic.




Sodium Phosphate, dibasic, heptahydrate, Reagent, ACS

Prod # Description Unit Price Order / Quote
19545 Sodium Phosphate, dibasic, heptahydrate, Reagent, ACS, 450g each $32.50
Qty:
I - MSDS (119KB PDF)
Na2HPO4 · 7H2O; [7782-85-6]; FW 268.07




Tissue-Tack™ Adhesive

Prod # Description Unit Price Order / Quote
19250 Tissue-Tack™ Adhesive, 100ml each $89.85
Qty:
I - MSDS (108KB PDF)
Permanent attachment of sections to glass slides during caustic or prolonged washes. For routine, immunofluorescent, immunoperoxidase staining.




Toluidine Blue O

Prod # Description Unit Price Order / Quote
19451 Toluidine Blue O, certified, 25g each $41.75
Qty:
I, T - MSDS (120KB PDF)
C15H16CLN3 S; [92-31-9]
For epoxy sections:

0.5% toluidine blue in 0.1% sodium carbonate solution at pH 11.1

For methacrylate/acrylic sections:
0.05% toluidine blue in benzoate buffer pH 4.4 (benzoic acid 0.25g, sodium benzoate 0.29g, water 200ml). p 5:3.11, "Procedures in Electron Microscopy".




Uranyl Acetate

Prod # Description Unit Price Order / Quote
19481 Uranyl Acetate, 25g each $189.00
Qty:
T, R (depleted) - MSDS (120KB PDF)
PELCO® Technical Notes, Uranium Salts for Staining, Uranyl Acetate (pdf)
UO2(CH3COO)2 - 2H2O; [6159-44-0]; FW 424.14
Positive or negative stain, EM. May cause adverse mutagenic or teratogenic effects.




Uranyl Acetate Alternative

Prod # Description Unit Price Order / Quote
19485 Uranyl Acetate Alternative, 25ml each $66.00
Qty:
MSDS (85KB PDF)
A Gadolinium Triacetate based stain as an alternative for Uranyl Acetate stain. Delivers comparable results without the authorization, shipping and disposal issue involved with Uranyl Acetate. Uranyl Acetate alternative is a non-radioactive stain; supplied as a concentrate. Works well for staining of plastic embedded animal and plant materials and a negative stain for macromolecules. Not recommended as a fixative.

Reference article: Masamichi Nakakoshi, Hedeo Nishioka and Eisaku Katayama. New versatile staining reagents for biological transmission electron microscopy that substitute for uranyl acetate. Journal of Electron Microscopy 60(6): 401-407. 2011.




Vinylec® E

(Formvar® substitute)

Prod # Description Unit Price Order / Quote
19223 Vinylec® E - Formvar® substitute, 100g each $12.00
Qty:
I - MSDS (93KB PDF)
Vinylec® E is a drop in substitute for Formvar® (polyvinyl formal). The original manufacturer (Monsanto) has sold parts of the business and the similar replacement product is now manufactured under the name Vinylec® E.
Vinylec® E (Formvar® substitute) is an excellent and one of the most widely used material to make support films on TEM grids.