Chemicals for Electron and Light Microscopy

Glutaraldehyde

EM grade purified glass distilled Glutaraldehyde sealed with inert gas in amber small-volume ampules, or in bottles for larger volumes.

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Prod #	Description	Unit	Price	Order / Quote
18420	Glutaraldehyde, 8% EM grade, 10 x 2ml	each	$19.70	Qty:
C, T, L - MSDS
18421	Glutaraldehyde, 8% EM grade, 10 x 10ml	each	20.90	Qty:
C, T, L - MSDS
18422	Glutaraldehyde, 8% EM grade, 100ml	each	21.40	Qty:
C, T, L - MSDS
18426	Glutaraldehyde, 25% EM grade, 10 x 10ml	each	31.40	Qty:
C, T, L - MSDS
18427	Glutaraldehyde, 25% EM grade, 100ml	each	24.80	Qty:
C, T, L - MSDS
18431	Glutaraldehyde, 50% EM grade, 10 x 10ml	each	49.65	Qty:
C, T, L - MSDS
18432	Glutaraldehyde, 50% EM grade, 100ml	each	49.20	Qty:
C, T, L - MSDS
18436	Glutaraldehyde, 70% EM grade, 10 x 2ml	each	37.30	Qty:
C, T, L - MSDS

Prod #	Description	Unit	Price	Order / Quote
18181	LR White Resin, Medium, 500ml	each	$119.90	Qty:

I - MSDS

Resin with catalyst packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR™ as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds). Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions could be seen on the complimentary surface. Epon sections show relatively smooth surfaces.18 Micrographs appear similar to freeze-etched images but scaled to nm instead of µm. 17 Carmichael SW: Down the resolution road: freeze-fracture revisited? Microscopy Today, 95-1, Jan/Feb 1995, p 3 18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470 Landmark Paper, Freeze-Fracture Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957 Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987. Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, V. 2, (Hayat MA, ed) PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod #	Description	Unit	Price	Order / Quote
18182	LR White Resin, Hard, 500ml	each	$128.95	Qty:

I - MSDS

Resin with catalyst packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly close BEEM® Capsules and PTFE Molds with ACLAR® as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results. PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Osmium Tetroxide

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Prod #	Description	Unit	Price	Order / Quote
18459	Osmium Tetroxide, 4% aqueous, 10 x 2ml	each	$63.60