Araldite 502 Embedding Kits

The Araldite 502 kit with BDMA accelerator is equally efficient as the same kit using DMP-30. BDMA has a lower viscosity and diffuses more rapidly into tissues, porous specimens and rough surfaces. The Araldite 502 resins have better beam stability than the Eponate resins. BDMA is used in 3% concentrations versus 1.5 to 2% for DMP-30. Araldite kit may be used alone or mixed with other epoxies. Curing times are 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave^®.
PELCO® Technical Notes, Araldite 502 Embedding Kits (263kb pdf )

1 Glauert AM, Glauert RH: Araldite as an embedding medium for electron microscopy. J Biophys Biochem Cytology, 4:191-194, 1958.
2 Glauert AM: Fixation, dehydration, and embedding of biological specimens. Practical Methods in Elec Micr, 3(1). Glauert AM, ed., North-Holland, Amsterdam, 5th reprint 1975.

Prod #	Description	Unit	Price	Order / Quote
18052	Araldite 502 Kit, 950ml (w/BDMA)	each	$39.06	Qty:

Prod #	Description	Unit	Price	Order / Quote
18050	Araldite 502 Kit, 950ml (w/DMP-30)	each	$42.00	Qty:

Eponate 12™ Kit, with BDMA

Aliphatic epoxy resin based on di- and tri-glydicyl ethers of glycerol with a significantly lower viscosity compared to Araldite 502. BDMA (<3%, see notes preceding) is used as the accelerator since it has lower viscosity and diffuses into tissues more rapidly. (See references below).
The viscosity of Eponate™ 12 at 25ºC is 15-21cP, WPE is 140–145 and Specific Gravity is 1.15–1.22g/ml. Other kit viscosities at 25ºC include DDSA (290cP) and NMA (225-275cP). Eponate™ 12 is hygroscopic.
Curing time is 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave^®.

First use of Epon 812 in U.S.A.:
Finck H, 1960. Epoxy resins in electron microscopy. J Biophys Biochem Cytol, 7, p. 27.
Luft JH, 1961. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol, 9, p. 409.

Prod #	Description	Unit	Price	Order / Quote
18012	Eponate 12 Embedding Kit, with BDMA	each	$71.51	Qty:

Prod #	Description	Unit	Price	Order / Quote
18010	Eponate 12 Kit (DMP-30 catalyst)	each	$73.04	Qty:

PELCO^® Technical Notes, PELCO^® Eponate 12™ Kit with DMP-30 and BDMA (PDF File)

Cg, l	Eponate 12 Resin, 450g (18005) MSDS
l	DDSA, 450g (18022) MSDS
C, I	NMA, 450g (18032) MSDS
T	DMP-30, 25g (18042) MSDS

NMA Hardener in increased amounts, in the embedding mixture, make a harder block.

Prod #	Description	Unit	Price	Order / Quote
18028	Eponate 12™ –Araldite 502 Kit	each	$67.82	Qty:

Cg, I	Eponate 12™ Resin, 450g (18005) MSDS
T, l	Araldite 502 Resin, 450g (18060) MSDS
I	DDSA, 450g (18022) MSDS
C, T, I. F	BDMA, 25g (18241) MSDS

PELCO^® Technical Notes, PELCO^® Araldite 502 Kit with DMP-30 and BDMA (PDF File)

Glycol Methacrylate (GMA) Kit

Prod #	Description	Unit	Price	Order / Quote
18350	Glycol methacrylate (GMA) Kit	each	$57.50	Qty:

F, l, T	Glycol Methacrylate (GMA), 450ml (18091) MSDS
F, l, T	n-Butyl Methacrylate, 225ml (18035) MSDS
O	Benzoyl Peroxide Paste (50%), 15g (18097) MSDS

JB-4^® Plus Embedding Kit

Prod #	Description	Unit	Price	Order / Quote
18040	JB4® Plus Embedding Kit	each	$273.35	Qty:

For making high resolution replication molds for casting "Spurr" resin go to President Replication Kit

Prod #	Description	Unit	Price	Order / Quote
18300-4221	Low Viscosity "Spurr" Kit	each	$62.40	Qty:

Cg	ERL 4221 (replaces ERL 4206), 225ml (18306-4221) MSDS
I	DER® 736, 250g (18310) MSDS
I	NSA, 450g (18301) MSDS
C	DMAE, 25g (18315) MSDS

Prod #	Description	Unit	Price	Order / Quote
18108	Low Viscosity "Spurr" Mini Kit	each	$81.20	Qty:

Note: See Full Details for Prod. No. 18108

PELCO^® Acrylic Embedding Resin Kit

A unique acrylic resin modeled after the UNICRYL^® acrylic resin. The advantage of this resin is that the catalyst is supplied separate from the resin components. Shelf life is improved and heat induced changes during shipping are eliminated.
This resin is ideally suited for LM and EM applications (immunocytochemistry, ultrastructural preservation, in situ hybridization with good cutting properties for either thick or thin sectioning). Unique cutting properties have been attributed to this resin formulation. It is felt that proteins, nucleic acids and macromolecules are revealed at the surface during sectioning. It is a hydrophilic resin which exhibits unique staining properties at the light level for a wide range of stains (i.e. Hemotoxylin, Silver Methenamine, Light Green, Eosin, Safranin, Toluidine Blue).
The resin contains Styrene Monomer (hazard, see MSDS), Monomeric (Meth)-acrylate Esters with Benzoyl Peroxide supplied as the initiator. Mixing of the initiator is required prior to use. The resin is best stored at -20°C after the addition of the initiator but the resin will remain liquid down to -50°C. It is a pale yellow liquid with a pungent odor that can be cured with either heat or UV radiation. Best results are attained when the resin is microwave cured. The resin should be capped during polymerization due to evaporation. Use proper gloves, fume hood and eye protection. PELCO® Acrylic Embedding Resin Kit, Technical Notes (1007 kb pdf)

Prod #	Description	Unit	Price	Order / Quote
18190	PELCO¨ Acrylic Embedding Resin Kit, 500ml	each	$148.50	Qty:

F, I, T	PELCO® Acrylic Embedding Resin Kit, 500ml MSDS
O, I	Benzoyl Peroxide Paste, 6.5g (18190-B) MSDS

Poly/Bed^® 812 Resin Test Kit

contains BDMA as accelerator

Contains BDMA in place of DMP-30 for faster infiltration.

Prod #	Description	Unit	Price	Order / Quote
18107	Poly-Bed® Test Kit	each	$68.00	Qty:

Low Viscosity "Spurr" Mini Kit

Prod #	Description	Unit	Price	Order / Quote
18108	Low Viscosity "Spurr" Test Kit	each	$81.20	Qty:

LR White Resin

Prod #	Description	Unit	Price	Order / Quote
18181	LR White Resin, Medium, 500ml	each	$134.60	Qty:

Resin with initiator (BPO) packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR™ as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds).

Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions could be seen on the complimentary surface. Epon sections show relatively smooth surfaces.18 Micrographs appear similar to freeze-etched images but scaled to nm instead of µm. Viscosity ~= 8cP

17 Carmichael SW: Down the resolution road: freeze-fracture revisited? Microscopy Today, 95-1, Jan/Feb 1995, p 3

18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470

Landmark Paper, Freeze-Fracture
Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957

Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987.
Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, V. 2, (Hayat MA, ed)

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182