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Embedding Kits

Embedding Chemicals for Electron Microscopy and Light Microscopy
Araldite 502
JB-4®
Low Viscosity "Spurr"
Glycol Methacrylate
  Poly/Bed® 812
Eponate 12™ Kits
LR White
Lowicryl® HM-20
 
Test Kits
Poly/Bed® Resin Test Kit
Low Viscosity "Spurr" Test Kit




Araldite 502 Embedding Kits

The Araldite 502 kit with BDMA accelerator is equally efficient as the same kit using DMP-30. BDMA has a lower viscosity and diffuses more rapidly into tissues, porous specimens and rough surfaces. The Araldite 502 resins have better beam stability than the Eponate resins. BDMA is used in 3% concentrations versus 1.5 to 2% for DMP-30. Araldite kit may be used alone or mixed with other epoxies. Curing times are 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave®.
PELCO® Technical Notes, Araldite 502 Embedding Kits (263kb pdf )

1 Glauert AM, Glauert RH: Araldite as an embedding medium for electron microscopy. J Biophys Biochem Cytology, 4:191-194, 1958.
2 Glauert AM: Fixation, dehydration, and embedding of biological specimens. Practical Methods in Elec Micr, 3(1). Glauert AM, ed., North-Holland, Amsterdam, 5th reprint 1975.

Prod # Description Unit Price Order / Quote
18052 Araldite 502 Kit, 950ml (w/BDMA) each $59.95
Qty:
Cg, I Araldite 502, 450ml (18060) MSDS (96KB PDF)
I DDSA, 450g (18022) MSDS (98KB PDF)
C, T, I, F BDMA, 25g (18241) MSDS (110KB PDF)

Prod # Description Unit Price Order / Quote
18050 Araldite 502 Kit, 950ml (w/DMP-30) each $54.95
Qty:
Cg, I Araldite 502, 450ml (18060) MSDS (96KB PDF)
I DDSA, 450g (18022) MSDS (98KB PDF)
C, I DMP-30, 25g (18042) MSDS (104KB PDF)




eponate 12 kit

Eponate 12™ Kit, with BDMA

Aliphatic epoxy resin based on di- and tri-glydicyl ethers of glycerol with a significantly lower viscosity compared to Araldite 502. BDMA (<3%, see notes preceding) is used as the accelerator since it has lower viscosity and diffuses into tissues more rapidly. (See references below).
The viscosity of Eponate™ 12 at 25ºC is 15-21cP, WPE is 140–145 and Specific Gravity is 1.15–1.22g/ml. Other kit viscosities at 25ºC include DDSA (290cP) and NMA (225-315cP). Eponate™ 12 is hygroscopic.
Curing time is 16 to 24 hours at a temperature of 60°C or 2 to 3 hours using the PELCO BioWave®.
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First use of Epon 812 in U.S.A.:
Finck H, 1960. Epoxy resins in electron microscopy. J Biophys Biochem Cytol, 7, p. 27.
Luft JH, 1961. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol, 9, p. 409.

Prod # Description Unit Price Order / Quote
18012 Eponate 12™ Embedding Kit, with BDMA each $130.65
Qty:

PELCO® Technical Notes, PELCO® Eponate 12™ Kit with DMP-30 and BDMA (PDF File)
Cg, I Eponate 12™ Resin, 450g (18005) MSDS (93KB PDF)
I DDSA, 450g (18022) MSDS (98KB PDF)
C, I
NMA, 450g (18032) MSDS (99KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
C, T, I, F BDMA, 25g (18241) MSDS (113KB PDF)




Eponate 12™ Embedding Kit, with DMP-30

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Prod # Description Unit Price Order / Quote
18010 Eponate 12 Kit (DMP-30 catalyst) each $112.50
Qty:

PELCO® Technical Notes, PELCO® Eponate 12™ Kit with DMP-30 and BDMA (PDF File)
Cg, I Eponate 12™ Resin, 450g (18005) MSDS (93KB PDF)
I DDSA, 450g (18022) MSDS (98KB PDF)
C, I
NMA, 450g (18032) MSDS (99KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
T DMP-30, 25g (18042) MSDS (104KB PDF)
NMA Hardener in increased amounts, in the embedding mixture, make a harder block.




Eponate 12™ –Araldite 502 Kit with BDMA

Prod # Description Unit Price Order / Quote
18028 Eponate 12™ –Araldite 502 Kit with BDMA each $115.65
Qty:
Cg, I Eponate 12™ Resin, 450g (18005) MSDS (93KB PDF)
T, I Araldite 502 Resin, 450g (18060) MSDS (96KB PDF)
I DDSA, 450g (18022) MSDS (98KB PDF)
C, T, I, F BDMA, 25g (18241) MSDS (113KB PDF)
PELCO® Technical Notes, PELCO® Araldite 502 Kit with DMP-30 and BDMA (PDF File)




Eponate 12™ –Araldite 502 Kit with DMP-30

Prod # Description Unit Price Order / Quote
18030 Eponate 12™ –Araldite 502 Kit with DMP-30 each $112.50
Qty:
Cg, I Eponate 12™ Resin, 450g (18005) MSDS (93KB PDF)
T, I Araldite 502 Resin, 450g (18060) MSDS (96KB PDF)
I DDSA, 450g (18022) MSDS (98KB PDF)
T DMP-30, 25g (18042) MSDS (104KB PDF)
PELCO® Technical Notes, PELCO® Araldite 502 Kit with DMP-30 and BDMA (PDF File)




Glycol Methacrylate (GMA) Kit

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Prod # Description Unit Price Order / Quote
18350 Glycol methacrylate (GMA) Kit each $59.75
Qty:
F, I, T Glycol Methacrylate (GMA), 450ml (18091) MSDS (108KB PDF)
F, I, T n-Butyl Methacrylate, 225ml (18035) MSDS (139KB PDF)
O Benzoyl Peroxide Paste (50%), 15g (18097) MSDS (111KB PDF)




JB-4® Embedding Kit

Based on Glycol Methacrylate (GMA) plastic embedding
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Prod # Description Unit Price Order / Quote
18020 JB4® Embedding Kit each $436.35
Qty:
F, I, T Solution A, 800ml (18023) MSDS (89KB PDF)
F, I, T Solution B, 30ml (18024) MSDS (110KB PDF)
O
Catalyst, 12g (18025) MSDS (94KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees




JB-4 Plus Embedding Kit

JB-4® Plus Embedding Kit

JB-4® PlusEmbedding Kit for light microscopy is a water soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. Its cooler acting accelerator system offers greater heat protection of tissue components. It yields semi-thin sections (0.5 - 2µm) with excellent morphological preservation. Crystal clear non-yellowing casts are obtained at room temperature in 90 minutes or less.
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Prod # Description Unit Price Order / Quote
18040 JB4® Plus Embedding Kit each $379.70
Qty:
F, I, T Monomer Solution A, 500ml (18043) MSDS (95KB PDF)
F, I, T Accelerator Solution B, 15ml (18044) MSDS (88KB PDF)
O
Catalyst, 8g (18045) MSDS (96KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees




"Spurr" Low Viscosity Kits

ERL 4221 has replaced ERL 4206. ERL 4221 has a higher viscosity than the previously used ERL 4206.

Spurr AR, 1969. A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruc Res, 26, p. 31.

Ellis, E Ann, Solutions to the Problem of Substitution of ERL 4221 for Vinyl Cyclohexene Dioxide in Spurr Low Viscosity Embedding Formulations, Microscopy Today, V 14, No 4, July 2006

The Spurr Kit gives excellent penetration for tough materials particularly in the studies of botanical, mineral and other hard biological specimens. When the mixture is kept at room temperature for 24 hours its viscosity will increase to 140cP, as is the case with other resins. When fully formulated, the Spurr’s resin will store in a deep-freeze for two months and at room temperature for two days. Curing is done at 70ºC for about 8 hours (see our Microwave system for significant time saving during epoxy processing).

For making high resolution replication molds for casting "Spurr" resin go to President Replication Kit

PELCO® Technical Notes for Low Viscosity Kit (PDF 1.54MB)

Prod # Description Unit Price Order / Quote
18300-4221 Low Viscosity "Spurr" Kit each $84.50
Qty:
Cg ERL 4221 (replaces ERL 4206), 225ml (18306-4221) MSDS (118KB PDF)
I DER® 736, 250g (18310) MSDS (99KB PDF)
I NSA, 450g (18301) MSDS (92KB PDF)
C DMAE, 25g (18315) MSDS (93KB PDF)

Prod # Description Unit Price Order / Quote
18108 Low Viscosity "Spurr" Mini Kit each $110.50
Qty:
Note: See Full Details for Prod. No. 18108




Lowicryl® Kit

MonoStep® Lowicryl® HM-20, Non-Polar

MSDS (91KB PDF) There are Hazard Shipping Fees associated with this product. Hazard Shipping Fees

Prod # Description Unit Price Order / Quote
18174 MonoStep® Lowicryl® HM-20, Non-Polar, 225g each $324.50
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Poly/Bed® 812 Embedding Kit, with DMP-30

Poly/Bed® 812 for electron microscopy and histology for studies requiring beam stability, low shrinkage and good contrast. Epoxy formulations are lower in viscosity than Araldite formulations, and hence provide better penetration into tissue samples.
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Prod # Description Unit Price Order / Quote
18109 Poly/Bed® 812 Kit (w/DMP-30) each $231.00
Qty:
Cg, I Poly/Bed® 812, 500g
I DDSA, 450g (18022) MSDS (98KB PDF)
C, I
NMA, 450g (18032) MSDS (99KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
T DMP-30, 100g
Instructions included.





Test Kits

Poly/Bed<sup>®</sup> 812 Resin Test Kit

Poly/Bed® 812 Resin Test Kit

contains BDMA as accelerator

Contains BDMA in place of DMP-30 for faster infiltration.

Prod # Description Unit Price Order / Quote
18107 Poly-Bed® Test Kit each $103.50
Qty:
I Poly/Bed® 812, 50ml MSDS (93KB PDF)
I DDSA, 40ml MSDS (96KB PDF)
T NMA, 25ml MSDS (100KB PDF)
C, T, I, F BDMA, 3.5ml MSDS (98KB PDF)
Instructions, Supplies, Syringes provided for leftovers. Mixture bottle provided.



low viscosity spurr test kit

Low Viscosity "Spurr" Mini Kit

Includes 92ml total consisting of ERL, DER® 736, NSA, DMAE and supplies for embedding.

Prod # Description Unit Price Order / Quote
18108 Low Viscosity "Spurr" Test Kit each $110.50
Qty:
Cg ERL 4221 (replaces ERL 4206), 20ml MSDS (118KB PDF)
I DER® 736, 20ml DER - MSDS (99KB PDF)
I NSA, 55ml NSA - MSDS 92KB PDF)
C DMAE, 5ml DMAE - MSDS (93KB PDF)

Low Viscosity "Spur" Mini Kit Technical Notes (pdf)







lr white

LR White Resin

Prod # Description Unit Price Order / Quote
18181 LR White Resin, Medium, 500ml each $154.00
Qty:
I - MSDS (107KB PDF) - MSDS (111KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees

Resin with initiator (BPO) packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR® as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin (see PTFE Molds).

Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen."17 A. Yamamoto and Y. Tashiro, using AFM, found a strong relief surface on LR White sections, 3-4nm on average. Depressions ranged from 6 to 30nm. Protrusions could be seen on the complimentary surface. Epon sections show relatively smooth surfaces.18 Micrographs appear similar to freeze-etched images but scaled to nm instead of µm. Viscosity ~= 8cP

18 Yamamoto A, Tashiro Y: Visualization by an atomic force microscope of the surface of ultra-thin sections of rat kidney and liver cells embedded in LR White. J Histochem Cytochem, 42, 1994, pp 1463-1470

Landmark Paper, Freeze-Fracture
Steere RL: Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol, 3, pp 45-60, 1957

Newman GR: Use and abuse of LR White. J. Histochem, 19, 118, 1987.
Newman GR: LR White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods, and Applications, V. 2, (Hayat MA, ed)

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod # Description Unit Price Order / Quote
18182 LR White Resin, Hard, 500ml each $154.00
Qty:
I - MSDS (107KB PDF) - MSDS (111KB PDF) - There are Hazard Shipping Fees associated with this product. - Hazard Shipping Fees
Resin with catalyst packaged separately. Accelerator 18185 can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly close BEEM® Capsules and PTFE Molds with ACLAR® as cover (Cat. No. 10506 or 10508) work well at 65°C. Widely used because of excellent immunolabeling results.

PELCO® Technical Notes, LR White Embedding Medium, Prod. Nos. 18181 & 18182

Prod # Description Unit Price Order / Quote
18185 Accelerator for LR white, 10ml each $19.50
Qty:
T- MSDS (89KB PDF)


Hazard Warning Guides Used in the Above Listings

Cg = Suspected Carcinogen
C = Corrosive
F = Flammable
I = Irritant
L = Lachrymator
O = Oxidizing
R = Radioactive
S = Sensitizer
T = Toxic
U = Unlisted as hazard; unknown hazard if any

An extra shipping charge is required by the Carriers when hazardous goods are transported.