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Calibration Overview
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TEM/STEM Test Specimens

Combined Test Specimen    Diffraction Standards    Image Rotation
Diffraction Grating Replicas    Gold Shadowed Latex


tem test specimen

Combined TEM Test Specimen - Gold / Graphitized Carbon

A holey carbon film is shadowed with gold and graphitized carbon particles are deposited. These particles viewed over the holes may be used for assessment of factors limiting the microscope performance. The evaporated gold forms small polycrystalline islands and within these islands lattice fringes can be resolved.

This specimen is also used for contamination measurement rates in the electron microscope by noting the deposition rate of carbon within the holes found in the gold film.

Prod # Description Unit Price Order / Quote
638 Combined TEM Test Specimen on 3mm grid each $86.25
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diffraction standard
aluminum

Diffraction Standard - Camera Length

Evaporated Aluminum

The nominal value of the effective camera length of an EM operating in the selected area mode is not sufficiently accurate for calculations of lattice spacing. The actual value of camera length must be calibrated at the same accelerating voltage and objective lens setting by reference to a known substance with well defined diffraction spacings. A normal specimen is evaporated film of aluminum with a thickness of around 31nm. Very small crystallite size yields ring patterns suitable for calibration. The specimen is supplied with a list of the principal lattice spacings. Made on a G400, 400 square mesh copper Gilder grid.

PELCO® Technical Notes, Diffraction Standard Evaporated Aluminum, Product No. 619 (PDF 417KB)

Prod # Description Unit Price Order / Quote
619 Evaporated Aluminum on 3mm grid each $24.50
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image rotation standard

Image Rotation

Molybdenum Trioxide

When changing from a selected area image of a specimen to a diffraction pattern, the strength of the intermediate lens is changed, producing an image rotation between the image and the diffraction pattern. This rotation is seen by photographing a crystal whose shape gives a clear indication of orientation. A molybdenum trioxide crystal is suitable for this purpose. Supported on a G400, 400 square mesh copper Gilder grid.
PELCO® Technical Notes, Image Rotation Molybdenum Trioxide Crystals, Product No. 625 (PDF 469KB)

Prod # Description Unit Price Order / Quote
625 Molybdenum Trioxide, on 3mm grid each $18.90
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image rotation standard

500nm Magnification Calibration Diffraction Grating Replica

Carbon replica with Au/Pd shadowing on a cross line grating with well-defined trench type grooves. Pitch or "d" spacing for parallel line grating is 500nm. The trench type groove makes it easy to measure the pitch. Typically, cross line grating is used up to 100,000x with 2,000 lines/mm in both directions. Instructions included. Made on a G400, 400 square mesh copper grid.
PELCO® Technical Notes, 2000 l/mm Cross Line Grating Replica, Product No. 677 (PDF 109KB)

Magnification Calibration Calculator (253)

Manufactured in USA
Prod # Description Unit Price Order / Quote
677 500nm Grating Replica, Cross line grating, 2000 l/mm, on 3mm grid each $45.50
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500nm Diffraction Grating Replica with Latex Spheres

image rotation standard image rotation standard

magnification calibration specimen

Two-in-one; this standard for calibration provides a double-check of the accuracy of magnification calibration. It is particularly useful at higher magnification; up to 150,000x. The latex sphere size is 261nm and the carbon cross line grating replica with Au/Pd shadowing has a pitch or "d" spacing of 500nm or 2000 lines/mm. Replica has well-defined trench type grooves, which makes it easy to measure the 500nm pitch. Supplied on a G400, 400 square mesh copper grid.
PELCO® Technical Notes, 500nm Diffraction Grating Replica with Latex Spheres, Product No. 673 (PDF 75KB)

Magnification Calibration Calculator (253)

Manufactured in USA
Prod # Description Unit Price Order / Quote
673 500nm Diffraction Grating Replica with Latex Spheres, 2000 l/mm, on 3mm grid each $45.90
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grating replica 606
606

waffle grating replica 607
607

PELCO® Technical Notes, Magnification Calibration Diffraction Grating Replica, Product No. 607 (pdf)

Magnification Calibration Calculator (252)

Manufactured in USA
Prod # Description Unit Price Order / Quote
606 Grating Replica, Parallel, 2160 l/mm, on 3mm grid each $43.90
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607 Grating Replica, Waffle, 2160 l/mm, on 3mm grid each 49.50
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diffraction grating replica

Diffraction
enlarged section

Diffraction Grating Replica with Latex Spheres

magnification calibration specimen

Two-in-one; this standard for calibration provides a double-check of the accuracy of magnification calibration. It is particularly useful at higher magnification. The latex sphere size is 0.261 µm and the carbon grating replica with Au/Pd shadowing is 2160 lines/mm. Supplied on a G400, 400 square mesh copper Gilder grid.

PELCO® Technical Notes, Magnification Calibration Latex Spheres on Diffraction Grating Replica (pdf)

Magnification Calibration Calculator (252)

Manufactured in USA
Prod # Description Unit Price Order / Quote
603 Diffraction Grating Replica and Latex Calibration Standard each $49.90
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gold shadowed latex

Gold Shadowed Latex

These are latex particles of 0.204µm diameter shadowed with a fairly heavy coating of gold. The gold forms islands of strongly scattering material and produces a suitable test object for STEM.
PELCO® Technical Notes, Gold Shadowed Latex Point Separation Resolution Test, Product No. 628-B (pdf)

Prod # Description Unit Price Order / Quote
628-B Gold Shadowed Latex, 3.05mm grid each $59.30
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