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Calibration Overview
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Lattice Plane Resolution Checks
for Transmission Electron Microscopy

Crystal lattice plane spacings are a good test of electron microscope stability, and provide an internal reference standard for magnification calibration.

tem resolution checks

Asbestos crocidolite
Plane spacing 0.9nm and 0.45nm

The 0.9nm spacing (020) will be found along the axis of the asbestos fibers. The 0.45nm spacing (021) appears at an angle of about 60° in suitable crystal orientations. To help prevent contamination of the EM or work area with asbestos fibers, these specimens are made using a substrate sandwich technique; the asbestos is layered between a carbon and Formvar film. Mounted on a standard 3mm TEM grid.

PELCO® Technical Notes, Lattice Plane Resolution Tests (PDF 741kb)

Prod # Description Unit Price Order / Quote
624 Asbestos (crocidolite) crystals, 3mm grid each $35.70
Qty:




graphitized carbon black

Graphitized Carbon Black
Plane Spacing 0.34nm

Graphitized Carbon Black is a stable and highly reproducible standard and is considered to be a standard resolution test for transmission electron microscopes. Mounted on a standard 3mm TEM grid.

PELCO® Technical Notes, Lattice Plane Resolution Tests (PDF 741kb)

Prod # Description Unit Price Order / Quote
645 Graphitized Carbon Black on 3mm grid each $41.45
Qty:




plane spacing

Oriented Single Crystal Gold Foil
Plane Spacing 0.204nm, 0.144nm and 0.102nm

Resolution, image quality, magnification and instrument stability in high performance TEMs can be checked by imaging the 0.204nm, 0.144nm and 0.104nm planar spacings in these specially prepared gold crystals. The tests are particularly recommended if height adjustments are made on the TEM specimen stage. The gold foil is mounted on a standard 3mm gold TEM grid.

Recommended storage: TEM Calibration Grid Holder

PELCO® Technical Notes, Lattice Plane Resolution Tests (PDF 741kb)

Prod # Description Unit Price Order / Quote
646 Oriented Gold Crystal on 3mm grid each $183.45
Qty: