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Catalase CrystalsCatalase crystals, negatively stained, are mounted on a grid. They display very clear lattice plane spacings in the TEM and STEM of 8.75nm and 6.85nm.Wrigley, J, (1968). Ultrastructure Res, 24, 454. Valuable for high magnification calibration. Technical Notes, Catalase Crystals (PDF 78KB) |
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FerritinResolution of the tetrad of this molecule indicates an instrument resolution better than 1.25nm. The ferritin is dispersed on a formvar/carbon substrate supported by a G400, 400 square mesh copper Gilder grid.PELCO® Technical Notes, Resolution Test Ferritin Molecules (PDF 399kb) |
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Gold on "Holey" Carbon FilmThe spaces formed between "islands" of evaporated gold are ideal for checking TEM/STEM (3mm grid) resolution. The holes allow for simultaneous astigmatism correction. Made on a G400, 400 square mesh copper Gilder grid. |
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Evaporated Platinum / IridiumEvaporated Pt/Ir on perforated holey carbon film. Support film provides holes for ease of focus and astigmatism correction. The grains of evaporated metal provide dense particles for TEM resolution checks by the particle separation test.PELCO® Technical Notes, Pt/Ir on Holey Carbon Film, Point Separation Resolution Test (PDF 395kb) |
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"Holey" (Perforated) Carbon Filmbest general TEM astigmatism checkThin films of carbon which have been treated to obtain a large number of small holes.
Supported on a G400, 400 square mesh copper Gilder grid. Examination of the Fresnel fringe around the hole when the objective lens is slightly defocused
enables astigmatism to be corrected. Observation of the fringe also gives an indication of TEM
stability and available resolution. |
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