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Polystyrene Latex Spheres
Used as a magnification test specimen in TEM and SEM, but is also employed in particle counting devices
as a size check and focus aid. Covers µm to nm range. Offered in a 10ml size, at 0.1% weight by volume.
Specific gravity is 1.05 g/ml. MSDS
| Sphere Size, Size Deviation, Sphere Density Table |
| Prod. No. |
Nom Size (µm) |
Size Uniformity (C.V.) |
Approx. Spheres/cc |
| 610-03 |
0.03 |
≤18% |
6.74E+13 |
| 610-08 |
0.08 |
≤15% |
3.55E+12 |
| 610-10 |
0.09 |
≤5% |
2.50E+12 |
| 610-14 |
0.17 |
≤3% |
3.70E+11 |
| 610-17 |
0.26 |
≤3% |
1.04E+11 |
| 610-20 |
0.30 |
≤3% |
6.74E+10 |
| 610-30 |
0.49 |
≤3% |
1.55E+10 |
| 610-38 |
1.00 |
≤3% |
1.82E+09 |
Actual size will appear on Product Label
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| Prod # | Description | Unit | Price | Order / Quote |
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| Latex Spheres, 0.03µm, nom | | |
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| Latex Spheres, 0.08µm, nom | | |
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| Latex Spheres, 0.09µm, nom | | |
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| Latex Spheres, 0.17µm, nom | | |
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| Latex Spheres, 0.26µm, nom | | |
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| Latex Spheres, 0.30µm, nom | | |
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| Latex Spheres, 0.49µm, nom | | |
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| Latex Spheres, 1.00µm, nom | | |
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| Set of latex spheres, 0.09, 0.30, and 0.48 µm mean diameters. |
| Latex Spheres, set of 3 | | |
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Certified Particle Size Polystyrene - Nanosphere Size Standards
These Nanosphere Size Standards are highly uniform polystyrene spheres calibrated in billionths
of a meter (nanometers) with NIST traceable methodology. One nanometer is 0.001 micrometer
(µm) or 10 Angstrom units.
Nanosphere Size Standards are packaged as aqueous suspensions in 15 milliliter (mL) dropper-tipped
bottles. The concentrations are optimized for ease of dispersion and colloidal stability. The spheres
have a density of 1.05 g/cm3 and an index of refraction of 1.59 @ 589nm (23°C).
Methods used to calibrate the diameter of the Nanospheres include adaptions of photon correlation
spectroscopy (PCS) and transmission electron microscopy (TEM). PCS uses scattered laser light to
correlate the diameter of suspended particles with their Brownian motion. PCS is also referred to
as quasi-elastic light scattering or dynamic light scattering.
Nanopshere Size Standards are ideal for the calibration of electron and atomic force microscopes.
They are also used in laser light-scattering studies and colloidal systems research. The 20 to 900nm
range of diameters is convenient for checking the sizes of bacteria, viruses, ribosomes and
sub-cellular components. MSDS
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| Sphere Size and Size Deviation Table |
| Prod. No. |
Nom
Diameter |
Certified
Mean Diameter |
Size Distribution
Std Dev. & C.V. |
Solids
Content |
| 610-50 |
20nm |
21nm ±1.5nm |
not determined |
1% |
| 610-53 |
50nm |
46nm ±2.0nm |
7.2nm (15.7%) |
1% |
| 610-56 |
80nm |
81nm ±2.7nm |
5.8nm (7.2%) |
1% |
| 610-58 |
100nm |
97nm ±3nm |
4.5nm (4.6%) |
1% |
| 610-60 |
150nm |
151nm ±4nm |
3.2nm (2.1%) |
1% |
| 610-61 |
200nm |
200nm ±6nm |
3.4nm (1.7%) |
1% |
| 610-63 |
240nm |
240nm ±6nm |
3.7nm (1.5%) |
1% |
| 610-66 |
350nm |
350nm ±7nm |
4.7nm (1.3%) |
1% |
| 610-69 |
500nm |
491nm ±4nm |
6.3nm (1.3%) |
1% |
| 610-73 |
600nm |
596nm ±6nm |
7.7nm (1.3%) |
1% |
| 610-76 |
900nm |
903nm ±9nm |
9.3nm (1.0%) |
1% |
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| Prod # | Description | Unit | Price | Order / Quote |
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| Certified Latex Nanospheres, 20nm | | |
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| Certified Latex Nanospheres, 50nm | | |
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| Certified Latex Nanospheres, 80nm | | |
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| Certified Latex Nanospheres, 100nm | | |
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| Certified Latex Nanospheres, 150nm | | |
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| Certified Latex Nanospheres, 200nm | | |
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| Certified Latex Nanospheres, 240nm | | |
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| Certified Latex Nanospheres, 350nm | | |
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| Certified Latex Nanospheres, 500nm | | |
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| Certified Latex Nanospheres, 600nm | | |
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| Certified Latex Nanospheres, 900nm | | |
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Catalase Crystals
Catalase crystals, negatively stained, are mounted on a grid.
They display very clear lattice plane spacings in the TEM and STEM of 8.75nm and 6.85nm.
Wrigley, J, (1968). Ultrastructure Res, 24, 454.
Valuable for high magnification calibration.
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| Prod # | Description | Unit | Price | Order / Quote |
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| Catalase Crystals, on 3mm grid | | |
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Resolution Test Specimens
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Ferritin
Resolution of the tetrad of this molecule indicates an instrument resolution better than 1.25nm.
The ferritin is dispersed on a formvar/carbon substrate supported by a copper mesh grid.
PELCO® Technical Notes, Resolution Test Ferritin Molecules (PDF 399kb)
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| Prod # | Description | Unit | Price | Order / Quote |
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| Ferritin on 3mm grid | | |
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Gold on "Holey" Carbon Film
The spaces formed between "islands" of evaporated gold are ideal for checking
TEM/STEM (3mm grid) resolution. The holes allow for simultaneous astigmatism correction.
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| Prod # | Description | Unit | Price | Order / Quote |
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| Gold on Holey Carbon Film | | |
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Evaporated Platinum / Iridium
Evaporated Pt/Ir on perforated holey carbon film. Support film provides holes for ease of focus and astigmatism correction.
The grains of evaporated metal provide dense particles for TEM resolution checks by the particle separation test.
PELCO® Technical Notes, Pt/Ir on Holey Carbon Film, Point Separation Resolution Test (PDF 395kb)
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| Prod # | Description | Unit | Price | Order / Quote |
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| Evaporated Platinum / Iridium; 3mm grid | | |
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