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Microwave-Assisted Decalcification

The work of Madden and Hensen, 1997 (Hearing Research, 111:76-84) was done using 0.1m EDTA in the microwave and was the first paper detailing the use of microwave-assisted decalcification of temporal bones with preservation of ultra structure.

A. Microwave-assisted decalcification saves from 10x to 100x of the time required by routine methods. It requires precise temperature control over long time periods of microwave irradiation (typically >30 minutes). Variable wattage, water recirculation and the temperature probe are required to achieve this type of environment.

B. Multiple microwave cycles are usually required to achieve complete decalcification of the sample. The use of dilute acids (i.e. nitric or formic) in place of EDTA will accelerate the process. The solution should be changed after each cycle.

C. The sample should be suspended in the decalcification solution, but do not cover the sample with >1cm of solution. Maintain the temperature restriction between 42-45°C for best results.

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